Morphological and immunohistochemical identification of neurons and their targets in the guinea-pig duodenum
Identifieur interne : 00DC20 ( Main/Merge ); précédent : 00DC19; suivant : 00DC21Morphological and immunohistochemical identification of neurons and their targets in the guinea-pig duodenum
Auteurs : N. Clerc [France] ; J. B. Furness [Australie] ; Z. S. Li [Australie] ; J. C. Bornstein [Australie] ; W. A. A. Kunze [Australie]Source :
- Neuroscience [ 0306-4522 ] ; 1998.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Nerve circuits within the proximal duodenum were investigated using a combination of immunohistochemistry for individual neuron markers and lesion of intrinsic nerve pathways to determine axon projections. Cell shapes and axonal projections were also studied in cells that had been injected with a marker substance. Several major neuron populations were identified. Calbindin immunoreacti;ity occurred in a population of myenteric nerve cells with Dogiel type II morphology. These had axons that projected to other myenteric ganglia, to the circular muscle and to the mucosa. All were immunoreactive for the synthesizing enzyme for acetylcholine, choline acetyltransferase, and some were also immunoreactive for calretinin. Myenteric neurons with nitric oxide synthase immunoreactivity ity projected anally to the circular muscle. These were also immunoreactive for vasoactive intestinal peptide, and proportions of them had enkephalin and/or neuropeptide Y immunoreactivity. It is suggested that they are inhibitory motor neurons to the circular muscle. A very few (about 2%) of nitric oxide synthase-immunoreactive neurons had choline acetyltransferase immunoreactivity. Tachykinin (substance P)-immunoreacti,c nerve cells were numerous in the myenteric plexus. Some of these projected orally to the circular muscle and are concluded to be excitatory motor neurons. Others projected to the tertiary plexus which innervates the longitudinal muscle and others provided terminals in the myenteric plexus. Two groups of descending interneurons were identified, one with somatostatin immunoreactivity and one with vasoactive intestinal peptide immunoreactivity. The two most common nerve cells in submucous ganglia were neuropeptide Y-and vasoactive intestinal peptide-immunoreactive nerve cells. Both provided innervation of the mucosa. There as also a population of calretinin-immunoreactive submucous neurons that innevated the mucosal glands, but not the villi. Comparison with the ileum reveals similarities in the chemistries and projections of neurons. Differences include the almost complete absence of nitric oxide synthase immunoreactivity from vasoactive intestinal peptide-immunoreactive interneurons in the duodenum. the projection of calbindin-immunoreactive Dogiel type II neurons to the circular muscle and the absence of tachykinin-immnunoreactivity from these neurons.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 006645
- to stream PascalFrancis, to step Curation: 006A17
- to stream PascalFrancis, to step Checkpoint: 006333
Links to Exploration step
Pascal:98-0361579Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Morphological and immunohistochemical identification of neurons and their targets in the guinea-pig duodenum</title><author><name sortKey="Clerc, N" sort="Clerc, N" uniqKey="Clerc N" first="N." last="Clerc">N. Clerc</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratoire de Neurobiologie, CNRS, 31 Ch J. Aiguier</s1><s2>13402 Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Furness, J B" sort="Furness, J B" uniqKey="Furness J" first="J. B." last="Furness">J. B. Furness</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Anatomy and Cell Biology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Li, Z S" sort="Li, Z S" uniqKey="Li Z" first="Z. S." last="Li">Z. S. Li</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Anatomy and Cell Biology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Bornstein, J C" sort="Bornstein, J C" uniqKey="Bornstein J" first="J. C." last="Bornstein">J. C. Bornstein</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Physiology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Kunze, W A A" sort="Kunze, W A A" uniqKey="Kunze W" first="W. A. A." last="Kunze">W. A. A. Kunze</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Anatomy and Cell Biology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">98-0361579</idno><date when="1998">1998</date><idno type="stanalyst">PASCAL 98-0361579 INIST</idno><idno type="RBID">Pascal:98-0361579</idno><idno type="wicri:Area/PascalFrancis/Corpus">006645</idno><idno type="wicri:Area/PascalFrancis/Curation">006A17</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">006333</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">006333</idno><idno type="wicri:doubleKey">0306-4522:1998:Clerc N:morphological:and:immunohistochemical</idno><idno type="wicri:Area/Main/Merge">00DC20</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Morphological and immunohistochemical identification of neurons and their targets in the guinea-pig duodenum</title><author><name sortKey="Clerc, N" sort="Clerc, N" uniqKey="Clerc N" first="N." last="Clerc">N. Clerc</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratoire de Neurobiologie, CNRS, 31 Ch J. Aiguier</s1><s2>13402 Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Furness, J B" sort="Furness, J B" uniqKey="Furness J" first="J. B." last="Furness">J. B. Furness</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Anatomy and Cell Biology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Li, Z S" sort="Li, Z S" uniqKey="Li Z" first="Z. S." last="Li">Z. S. Li</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Anatomy and Cell Biology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Bornstein, J C" sort="Bornstein, J C" uniqKey="Bornstein J" first="J. C." last="Bornstein">J. C. Bornstein</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Physiology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Kunze, W A A" sort="Kunze, W A A" uniqKey="Kunze W" first="W. A. A." last="Kunze">W. A. A. Kunze</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Anatomy and Cell Biology, University of Melbourne</s1><s2>Parkville, Victoria 5042</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author></analytic><series><title level="j" type="main">Neuroscience</title><title level="j" type="abbreviated">Neuroscience</title><idno type="ISSN">0306-4522</idno><imprint><date when="1998">1998</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Neuroscience</title><title level="j" type="abbreviated">Neuroscience</title><idno type="ISSN">0306-4522</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Duodenum</term><term>Enteric nervous system</term><term>Guinea pig</term><term>Immunohistochemistry</term><term>Innervation</term><term>Morphology</term><term>Neuron</term><term>Nitric-oxide synthase</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Immunohistochimie</term><term>Duodénum</term><term>Neurone</term><term>Système nerveux entérique</term><term>Innervation</term><term>Nitric-oxide synthase</term><term>Morphologie</term><term>Cobaye</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nerve circuits within the proximal duodenum were investigated using a combination of immunohistochemistry for individual neuron markers and lesion of intrinsic nerve pathways to determine axon projections. Cell shapes and axonal projections were also studied in cells that had been injected with a marker substance. Several major neuron populations were identified. Calbindin immunoreacti;ity occurred in a population of myenteric nerve cells with Dogiel type II morphology. These had axons that projected to other myenteric ganglia, to the circular muscle and to the mucosa. All were immunoreactive for the synthesizing enzyme for acetylcholine, choline acetyltransferase, and some were also immunoreactive for calretinin. Myenteric neurons with nitric oxide synthase immunoreactivity ity projected anally to the circular muscle. These were also immunoreactive for vasoactive intestinal peptide, and proportions of them had enkephalin and/or neuropeptide Y immunoreactivity. It is suggested that they are inhibitory motor neurons to the circular muscle. A very few (about 2%) of nitric oxide synthase-immunoreactive neurons had choline acetyltransferase immunoreactivity. Tachykinin (substance P)-immunoreacti,c nerve cells were numerous in the myenteric plexus. Some of these projected orally to the circular muscle and are concluded to be excitatory motor neurons. Others projected to the tertiary plexus which innervates the longitudinal muscle and others provided terminals in the myenteric plexus. Two groups of descending interneurons were identified, one with somatostatin immunoreactivity and one with vasoactive intestinal peptide immunoreactivity. The two most common nerve cells in submucous ganglia were neuropeptide Y-and vasoactive intestinal peptide-immunoreactive nerve cells. Both provided innervation of the mucosa. There as also a population of calretinin-immunoreactive submucous neurons that innevated the mucosal glands, but not the villi. Comparison with the ileum reveals similarities in the chemistries and projections of neurons. Differences include the almost complete absence of nitric oxide synthase immunoreactivity from vasoactive intestinal peptide-immunoreactive interneurons in the duodenum. the projection of calbindin-immunoreactive Dogiel type II neurons to the circular muscle and the absence of tachykinin-immnunoreactivity from these neurons.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li><li>Victoria (État)</li></region><settlement><li>Marseille</li><li>Melbourne</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Clerc, N" sort="Clerc, N" uniqKey="Clerc N" first="N." last="Clerc">N. Clerc</name></region></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Furness, J B" sort="Furness, J B" uniqKey="Furness J" first="J. B." last="Furness">J. B. Furness</name></region><name sortKey="Bornstein, J C" sort="Bornstein, J C" uniqKey="Bornstein J" first="J. C." last="Bornstein">J. C. Bornstein</name><name sortKey="Kunze, W A A" sort="Kunze, W A A" uniqKey="Kunze W" first="W. A. A." last="Kunze">W. A. A. Kunze</name><name sortKey="Li, Z S" sort="Li, Z S" uniqKey="Li Z" first="Z. S." last="Li">Z. S. Li</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00DC20 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 00DC20 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Merge
|type= RBID
|clé= Pascal:98-0361579
|texte= Morphological and immunohistochemical identification of neurons and their targets in the guinea-pig duodenum
}}
| This area was generated with Dilib version V0.6.33. |  |