Modification of articular cartilage and subchondral bone pathology in an ovine meniscectomy model of osteoarthritis by avocado and soya unsaponifiables (ASU)
Identifieur interne : 00CE82 ( Main/Merge ); précédent : 00CE81; suivant : 00CE83Modification of articular cartilage and subchondral bone pathology in an ovine meniscectomy model of osteoarthritis by avocado and soya unsaponifiables (ASU)
Auteurs : M. A Cake [Australie] ; R. A Read [Australie] ; B. Guillou [France] ; P. Ghosh [Australie]Source :
- Osteoarthritis and Cartilage [ 1063-4584 ] ; 2000.
Descripteurs français
- Wicri :
- topic : Analyse quantitative.
English descriptors
- KwdEn :
- ASU, Osteoarthritis, Cartilage, Image analysis, Animal models, Arthritis, Arthritis rheum, Articular, Articular cartilage, Articular cartilage proteoglycan content, Birkhauser verlag, Body weights, Cartilage, Cartilage area, Cartilage degeneration, Cartilage erosion, Cartilage integrity, Cartilage pathology table, Cartilage surface, Cartilage thickness, Chondrocytes, Chondrocytes articulaires, Clin orthop, Clinical trials, Collagen network, Computerized image analysis, Condyle, Control animals, Cultured articular chondrocytes, Deleterious effects, Exhibit activity, Experimental osteoarthrosis, Femoral, Femoral condyle, Fibrillation erosion, Full depth, Ghosh, Growth factor, Histological, Histomorphometric, Histomorphometric assessment, Histomorphometric methods, Histopathological, Histopathological scores, Image analysis, Inner zone, Joint changes, Joint destruction, Joint regions, Joint research, Laboratoires pharmascience, Lateral, Lateral compartment, Lateral femoral condyle, Lateral femoral condyles, Lateral meniscectomy, Lateral tibial plateau, Matrix, Matrix production, Medial, Medial compartment, Meniscectomized, Meniscectomized animals, Meniscectomy, Menx, Menx animals, Middle zone, Murdoch university, Ndings support, Osteoarthritic, Osteoarthritic joints, Osteoarthritis, Osteoarthritis cart, Osteoarthritis research society, Osteophyte, Osteophyte development, Outer zone, Placebo, Plateau, Present study, Proteoglycan, Quantitative analysis, Radial zone depth, Relevant menx value, Rheum, Shore hospital, Slight decrease, Subchondral, Subchondral bone, Subchondral bone sclerosis, Subchondral plate thickness, Subchondral sclerosis, Surface irregularities, Symptomatic efficacy, Tibial, Tibial plateaux, Toluidine, Toluidine section, Transitional zone depth, Treatment groups, Trochlear groove, Weight gain, Zone.
- Teeft :
- Animal models, Arthritis, Arthritis rheum, Articular, Articular cartilage, Articular cartilage proteoglycan content, Birkhauser verlag, Body weights, Cartilage, Cartilage area, Cartilage degeneration, Cartilage erosion, Cartilage integrity, Cartilage pathology table, Cartilage surface, Cartilage thickness, Chondrocytes, Chondrocytes articulaires, Clin orthop, Clinical trials, Collagen network, Computerized image analysis, Condyle, Control animals, Cultured articular chondrocytes, Deleterious effects, Exhibit activity, Experimental osteoarthrosis, Femoral, Femoral condyle, Fibrillation erosion, Full depth, Ghosh, Growth factor, Histological, Histomorphometric, Histomorphometric assessment, Histomorphometric methods, Histopathological, Histopathological scores, Image analysis, Inner zone, Joint changes, Joint destruction, Joint regions, Joint research, Laboratoires pharmascience, Lateral, Lateral compartment, Lateral femoral condyle, Lateral femoral condyles, Lateral meniscectomy, Lateral tibial plateau, Matrix, Matrix production, Medial, Medial compartment, Meniscectomized, Meniscectomized animals, Meniscectomy, Menx, Menx animals, Middle zone, Murdoch university, Ndings support, Osteoarthritic, Osteoarthritic joints, Osteoarthritis, Osteoarthritis cart, Osteoarthritis research society, Osteophyte, Osteophyte development, Outer zone, Placebo, Plateau, Present study, Proteoglycan, Quantitative analysis, Radial zone depth, Relevant menx value, Rheum, Shore hospital, Slight decrease, Subchondral, Subchondral bone, Subchondral bone sclerosis, Subchondral plate thickness, Subchondral sclerosis, Surface irregularities, Symptomatic efficacy, Tibial, Tibial plateaux, Toluidine, Toluidine section, Transitional zone depth, Treatment groups, Trochlear groove, Weight gain, Zone.
Abstract
Abstract: Objective To examine the effect of an oral preparation of avocado and soya unsaponifiables (ASU) on the development of joint pathology in an ovine model of osteoarthritis (OA), using computer-assisted histomorphometric methods. Design OA was induced in ovine knee joints by bilateral lateral meniscectomy (N=32). ASU (900mg/weekday) was given orally to half the group (MenX+ASU), the remainder receiving placebo (MenX). Sixteen animals were used as non-operated controls (NOC). At 3 and 6 months post-meniscectomy, histological sections from the medial and lateral femoral condyles (MFC, LFC), tibial plateaux (MTP, LTP) and trochlear groove (TG) were prepared from all joints. Sections were scored using traditional histopathological scales, and computerized image analysis, measuring total cartilage area, uncalcified cartilage (UCC) and subchondral bone plate (SCP) thickness, and intensity of articular cartilage toluidine blue staining (mean greyscale intensity, black=255) as an index of proteoglycan (PG) content. Results Computerized image analysis showed significant histological differences not detectable by traditional scoring methods. ASU-treated animals at 6 months showed reduced loss of toluidine blue stain in the MTP (P=0.015) and LTP (P=0.001), and significantly greater staining in the TG than either placebo or NOC groups (P=0.011). UCC thickness increased after meniscectomy, but tended to be highest in ASU-treated animals, significantly so in the middle zone of the LFC (MenX+ASU: 1.03±0.21mm vs MenX: 0.79±0.14mm,P =0.018; NOC: 0.77±0.17mm). Lateral compartment SCP thickness increased post-meniscectomy but was increased significantly less in the inner zone of the LTP in ASU-treated sheep (MenX+ASU: 1.37±0.23mm vs MenX: 1.68±0.28mm, P=0.033; NOC=1.22±0.33mm). Conclusions In this model ASU treatment following meniscectomy appeared to confer a subtle but statistically significant protective effect on articular cartilage. Although the drug failed to prevent focal cartilage lesions, characteristic of this model, histomorphometric analysis demonstrated greater PG content and UCC thickness in adjacent joint regions of ASU-treated animals. In addition, a statistically significant reduction of subchondral bone sclerosis was noted in the LTP region of the drug-treated group. An anabolic effect on chondrocytes, resulting in the stimulation of matrix production in regions distant to the insult, was also suggested by the data. These findings support other studies which have proposed that ASU may exhibit disease-modifying anti-OA activity.
Url:
DOI: 10.1053/joca.1999.0315
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ISTEX:2A9DBA33A223BC15036DB9DF13D4384D1C72F299Le document en format XML
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<term>Arthritis</term>
<term>Arthritis rheum</term>
<term>Articular</term>
<term>Articular cartilage</term>
<term>Articular cartilage proteoglycan content</term>
<term>Birkhauser verlag</term>
<term>Body weights</term>
<term>Cartilage</term>
<term>Cartilage area</term>
<term>Cartilage degeneration</term>
<term>Cartilage erosion</term>
<term>Cartilage integrity</term>
<term>Cartilage pathology table</term>
<term>Cartilage surface</term>
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<term>Chondrocytes</term>
<term>Chondrocytes articulaires</term>
<term>Clin orthop</term>
<term>Clinical trials</term>
<term>Collagen network</term>
<term>Computerized image analysis</term>
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<term>Control animals</term>
<term>Cultured articular chondrocytes</term>
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<term>Exhibit activity</term>
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<term>Histopathological scores</term>
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<term>Joint changes</term>
<term>Joint destruction</term>
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<term>Osteoarthritis research society</term>
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<term>Osteophyte development</term>
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<term>Placebo</term>
<term>Plateau</term>
<term>Present study</term>
<term>Proteoglycan</term>
<term>Quantitative analysis</term>
<term>Radial zone depth</term>
<term>Relevant menx value</term>
<term>Rheum</term>
<term>Shore hospital</term>
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<term>Subchondral bone</term>
<term>Subchondral bone sclerosis</term>
<term>Subchondral plate thickness</term>
<term>Subchondral sclerosis</term>
<term>Surface irregularities</term>
<term>Symptomatic efficacy</term>
<term>Tibial</term>
<term>Tibial plateaux</term>
<term>Toluidine</term>
<term>Toluidine section</term>
<term>Transitional zone depth</term>
<term>Treatment groups</term>
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<term>Weight gain</term>
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<term>Histopathological scores</term>
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<front><div type="abstract" xml:lang="en">Abstract: Objective To examine the effect of an oral preparation of avocado and soya unsaponifiables (ASU) on the development of joint pathology in an ovine model of osteoarthritis (OA), using computer-assisted histomorphometric methods. Design OA was induced in ovine knee joints by bilateral lateral meniscectomy (N=32). ASU (900mg/weekday) was given orally to half the group (MenX+ASU), the remainder receiving placebo (MenX). Sixteen animals were used as non-operated controls (NOC). At 3 and 6 months post-meniscectomy, histological sections from the medial and lateral femoral condyles (MFC, LFC), tibial plateaux (MTP, LTP) and trochlear groove (TG) were prepared from all joints. Sections were scored using traditional histopathological scales, and computerized image analysis, measuring total cartilage area, uncalcified cartilage (UCC) and subchondral bone plate (SCP) thickness, and intensity of articular cartilage toluidine blue staining (mean greyscale intensity, black=255) as an index of proteoglycan (PG) content. Results Computerized image analysis showed significant histological differences not detectable by traditional scoring methods. ASU-treated animals at 6 months showed reduced loss of toluidine blue stain in the MTP (P=0.015) and LTP (P=0.001), and significantly greater staining in the TG than either placebo or NOC groups (P=0.011). UCC thickness increased after meniscectomy, but tended to be highest in ASU-treated animals, significantly so in the middle zone of the LFC (MenX+ASU: 1.03±0.21mm vs MenX: 0.79±0.14mm,P =0.018; NOC: 0.77±0.17mm). Lateral compartment SCP thickness increased post-meniscectomy but was increased significantly less in the inner zone of the LTP in ASU-treated sheep (MenX+ASU: 1.37±0.23mm vs MenX: 1.68±0.28mm, P=0.033; NOC=1.22±0.33mm). Conclusions In this model ASU treatment following meniscectomy appeared to confer a subtle but statistically significant protective effect on articular cartilage. Although the drug failed to prevent focal cartilage lesions, characteristic of this model, histomorphometric analysis demonstrated greater PG content and UCC thickness in adjacent joint regions of ASU-treated animals. In addition, a statistically significant reduction of subchondral bone sclerosis was noted in the LTP region of the drug-treated group. An anabolic effect on chondrocytes, resulting in the stimulation of matrix production in regions distant to the insult, was also suggested by the data. These findings support other studies which have proposed that ASU may exhibit disease-modifying anti-OA activity.</div>
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