AustralieFrV1, Main, Merge, bibRecord, 007B78

Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage

Identifieur interne : 007B78 ( Main/Merge ); précédent : 007B77; suivant : 007B79

Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage

Auteurs : Badrish Soni [France, Australie] ; Nishant P. Visavadiya [Inde, États-Unis] ; Nirav Dalwadi [Inde] ; Datta Madamwar [Inde] ; Chris Winder [Australie] ; Christian Khalil [Australie]

Source :

Journal of applied toxicology [ 0260-437X ] ; 2010.

RBID : Pascal:10-0414984

Descripteurs français

Pascal (Inist)
- Permanganate de potassium, Toxicité, Animal, Rat, Prévention, Fibroblaste, Lésion, Aigu, Chronique, Test comète, Phormidium, Potassium Permanganate.

English descriptors

KwdEn :
- Acute, Animal, Chronic, Comet assay, Fibroblast, Lesion, Phormidium, Potassium Permanganates, Prevention, Rat, Toxicity.

Abstract

We have evaluated in vitro cytotoxicity of cyanobacterial phycoerythrin (C-PE) on three human cell lines by cell proliferation and neutral red uptake assays. No toxic effects of C-PE were observed to any of the cell lines tested. The protective role of purified C-PE to potassium permanganate-mediated human fibroblast-DNA damage was assessed by comet assay at 0 (control), 10 and 20 μg C-PE ml^-1 doses in pre-, simultaneous and post-mutagen exposure conditions. Significant DNA damage was detected only in post-mutagen exposure conditions. Our findings confirmed that the C-PE is non-toxic and provides protection against permanganate-mediated DNA damage. The preliminary acute (2000 mg C-PE kg^-1 body weight, b.w.) and 90 day sub-chronic (0, 5, 15 and 25 mg C-PE kg^-1 b.w./day) oral toxicity studies of purified C-PE in male albino rats showed no mortality or treatment-related major clinical signs, and all the doses of C-PE were well tolerated. The no observed adverse effect level and no observed effect level were found to be 15 and 5 mg C-PE kg^-1 b.w./day respectively.

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 002408
to stream PascalFrancis, to step Curation: 003B85
to stream PascalFrancis, to step Checkpoint: 002110

Links to Exploration step

Pascal:10-0414984

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage</title>
<author><name sortKey="Soni, Badrish" sort="Soni, Badrish" uniqKey="Soni B" first="Badrish" last="Soni">Badrish Soni</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratoire de Biologie Integrative, CEA Saclay</s1>
<s2>91191 Gif Sur Yvette</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Gif Sur Yvette</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Chemical Safety and Applied Toxicology Laboratories, School of Risk and Safety Sciences, The University of New South Wales</s1>
<s2>Sydney</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Visavadiya, Nishant P" sort="Visavadiya, Nishant P" uniqKey="Visavadiya N" first="Nishant P." last="Visavadiya">Nishant P. Visavadiya</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>BRD School of Biosciences, Sardar Patel University</s1>
<s2>Gujarat</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Gujarat</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Graduate Center for Nutritional Sciences, University of Kentucky</s1>
<s2>Lexington, Kentucky 40536</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lexington, Kentucky 40536</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dalwadi, Nirav" sort="Dalwadi, Nirav" uniqKey="Dalwadi N" first="Nirav" last="Dalwadi">Nirav Dalwadi</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>BRD School of Biosciences, Sardar Patel University</s1>
<s2>Gujarat</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Gujarat</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Madamwar, Datta" sort="Madamwar, Datta" uniqKey="Madamwar D" first="Datta" last="Madamwar">Datta Madamwar</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>BRD School of Biosciences, Sardar Patel University</s1>
<s2>Gujarat</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Gujarat</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Winder, Chris" sort="Winder, Chris" uniqKey="Winder C" first="Chris" last="Winder">Chris Winder</name>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Chemical Safety and Applied Toxicology Laboratories, School of Risk and Safety Sciences, The University of New South Wales</s1>
<s2>Sydney</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Khalil, Christian" sort="Khalil, Christian" uniqKey="Khalil C" first="Christian" last="Khalil">Christian Khalil</name>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Chemical Safety and Applied Toxicology Laboratories, School of Risk and Safety Sciences, The University of New South Wales</s1>
<s2>Sydney</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">10-0414984</idno>
<date when="2010">2010</date>
<idno type="stanalyst">PASCAL 10-0414984 INIST</idno>
<idno type="RBID">Pascal:10-0414984</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">002408</idno>
<idno type="wicri:Area/PascalFrancis/Curation">003B85</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">002110</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">002110</idno>
<idno type="wicri:doubleKey">0260-437X:2010:Soni B:purified:c:phycoerythrin</idno>
<idno type="wicri:Area/Main/Merge">007B78</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage</title>
<author><name sortKey="Soni, Badrish" sort="Soni, Badrish" uniqKey="Soni B" first="Badrish" last="Soni">Badrish Soni</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratoire de Biologie Integrative, CEA Saclay</s1>
<s2>91191 Gif Sur Yvette</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Gif Sur Yvette</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Chemical Safety and Applied Toxicology Laboratories, School of Risk and Safety Sciences, The University of New South Wales</s1>
<s2>Sydney</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Visavadiya, Nishant P" sort="Visavadiya, Nishant P" uniqKey="Visavadiya N" first="Nishant P." last="Visavadiya">Nishant P. Visavadiya</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>BRD School of Biosciences, Sardar Patel University</s1>
<s2>Gujarat</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Gujarat</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Graduate Center for Nutritional Sciences, University of Kentucky</s1>
<s2>Lexington, Kentucky 40536</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lexington, Kentucky 40536</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dalwadi, Nirav" sort="Dalwadi, Nirav" uniqKey="Dalwadi N" first="Nirav" last="Dalwadi">Nirav Dalwadi</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>BRD School of Biosciences, Sardar Patel University</s1>
<s2>Gujarat</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Gujarat</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Madamwar, Datta" sort="Madamwar, Datta" uniqKey="Madamwar D" first="Datta" last="Madamwar">Datta Madamwar</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>BRD School of Biosciences, Sardar Patel University</s1>
<s2>Gujarat</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Gujarat</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Winder, Chris" sort="Winder, Chris" uniqKey="Winder C" first="Chris" last="Winder">Chris Winder</name>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Chemical Safety and Applied Toxicology Laboratories, School of Risk and Safety Sciences, The University of New South Wales</s1>
<s2>Sydney</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Khalil, Christian" sort="Khalil, Christian" uniqKey="Khalil C" first="Christian" last="Khalil">Christian Khalil</name>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Chemical Safety and Applied Toxicology Laboratories, School of Risk and Safety Sciences, The University of New South Wales</s1>
<s2>Sydney</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of applied toxicology</title>
<title level="j" type="abbreviated">J. appl. toxicol.</title>
<idno type="ISSN">0260-437X</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of applied toxicology</title>
<title level="j" type="abbreviated">J. appl. toxicol.</title>
<idno type="ISSN">0260-437X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term>
<term>Animal</term>
<term>Chronic</term>
<term>Comet assay</term>
<term>Fibroblast</term>
<term>Lesion</term>
<term>Phormidium</term>
<term>Potassium Permanganates</term>
<term>Prevention</term>
<term>Rat</term>
<term>Toxicity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Permanganate de potassium</term>
<term>Toxicité</term>
<term>Animal</term>
<term>Rat</term>
<term>Prévention</term>
<term>Fibroblaste</term>
<term>Lésion</term>
<term>Aigu</term>
<term>Chronique</term>
<term>Test comète</term>
<term>Phormidium</term>
<term>Potassium Permanganate</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We have evaluated in vitro cytotoxicity of cyanobacterial phycoerythrin (C-PE) on three human cell lines by cell proliferation and neutral red uptake assays. No toxic effects of C-PE were observed to any of the cell lines tested. The protective role of purified C-PE to potassium permanganate-mediated human fibroblast-DNA damage was assessed by comet assay at 0 (control), 10 and 20 μg C-PE ml<sup>-1</sup>
 doses in pre-, simultaneous and post-mutagen exposure conditions. Significant DNA damage was detected only in post-mutagen exposure conditions. Our findings confirmed that the C-PE is non-toxic and provides protection against permanganate-mediated DNA damage. The preliminary acute (2000 mg C-PE kg<sup>-1</sup>
 body weight, b.w.) and 90 day sub-chronic (0, 5, 15 and 25 mg C-PE kg<sup>-1</sup>
 b.w./day) oral toxicity studies of purified C-PE in male albino rats showed no mortality or treatment-related major clinical signs, and all the doses of C-PE were well tolerated. The no observed adverse effect level and no observed effect level were found to be 15 and 5 mg C-PE kg<sup>-1</sup>
 b.w./day respectively.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Inde</li>
<li>États-Unis</li>
</country>
<region><li>Nouvelle-Galles du Sud</li>
<li>Île-de-France</li>
</region>
<settlement><li>Gif Sur Yvette</li>
<li>Sydney</li>
</settlement>
</list>
<tree><country name="France"><region name="Île-de-France"><name sortKey="Soni, Badrish" sort="Soni, Badrish" uniqKey="Soni B" first="Badrish" last="Soni">Badrish Soni</name>
</region>
</country>
<country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Soni, Badrish" sort="Soni, Badrish" uniqKey="Soni B" first="Badrish" last="Soni">Badrish Soni</name>
</region>
<name sortKey="Khalil, Christian" sort="Khalil, Christian" uniqKey="Khalil C" first="Christian" last="Khalil">Christian Khalil</name>
<name sortKey="Winder, Chris" sort="Winder, Chris" uniqKey="Winder C" first="Chris" last="Winder">Chris Winder</name>
</country>
<country name="Inde"><noRegion><name sortKey="Visavadiya, Nishant P" sort="Visavadiya, Nishant P" uniqKey="Visavadiya N" first="Nishant P." last="Visavadiya">Nishant P. Visavadiya</name>
</noRegion>
<name sortKey="Dalwadi, Nirav" sort="Dalwadi, Nirav" uniqKey="Dalwadi N" first="Nirav" last="Dalwadi">Nirav Dalwadi</name>
<name sortKey="Madamwar, Datta" sort="Madamwar, Datta" uniqKey="Madamwar D" first="Datta" last="Madamwar">Datta Madamwar</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Visavadiya, Nishant P" sort="Visavadiya, Nishant P" uniqKey="Visavadiya N" first="Nishant P." last="Visavadiya">Nishant P. Visavadiya</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 007B78 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 007B78 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     Pascal:10-0414984
   |texte=   Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage

Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri