Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein*
Identifieur interne : 006398 ( Main/Merge ); précédent : 006397; suivant : 006399Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein*
Auteurs : Yohann Demont ; Cyril Corbet ; Adeline Page ; Yasemin Ataman-Önal ; Genevieve Choquet-Kastylevsky ; Ingrid Fliniaux ; Xuefen Le Bourhis ; Robert-Alain Toillon ; Ralph A. Bradshaw ; Hubert HondermarckSource :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2011.
Abstract
The precursor of nerve growth factor (proNGF) has been described as a biologically active polypeptide able to induce apoptosis in neuronal cells, via the neurotrophin receptor p75NTR and the sortilin receptor. Herein, it is shown that proNGF is produced and secreted by breast cancer cells, stimulating their invasion. Using Western blotting and mass spectrometry, proNGF was detected in a panel of breast cancer cells as well as in their conditioned media. Immunohistochemical analysis indicated an overproduction of proNGF in breast tumors, when compared with benign and normal breast biopsies, and a relationship to lymph node invasion in ductal carcinomas. Interestingly, siRNA against proNGF induced a decrease of breast cancer cell invasion that was restored by the addition of non-cleavable proNGF. The activation of TrkA, Akt, and Src, but not the MAP kinases, was observed. In addition, the proNGF invasive effect was inhibited by the Trk pharmacological inhibitor K252a, a kinase-dead TrkA, and siRNA against TrkA sortilin, neurotensin, whereas siRNA against p75NTR and the MAP kinase inhibitor PD98059 had no impact. These data reveal the existence of an autocrine loop stimulated by proNGF and mediated by TrkA and sortilin, with the activation of Akt and Src, for the stimulation of breast cancer cell invasion.
Url:
DOI: 10.1074/jbc.M110.211714
PubMed: 22128158
PubMed Central: 3265873
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001916
- to stream Pmc, to step Curation: 001775
- to stream Pmc, to step Checkpoint: 002198
- to stream Ncbi, to step Merge: 000C45
- to stream Ncbi, to step Curation: 000C45
- to stream Ncbi, to step Checkpoint: 000C45
Links to Exploration step
PMC:3265873Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein<xref ref-type="fn" rid="FN1">*</xref>
<xref ref-type="fn" rid="FN2"><sup><inline-graphic xlink:href="sbox.jpg"></inline-graphic>
</sup>
</xref>
</title>
<author><name sortKey="Demont, Yohann" sort="Demont, Yohann" uniqKey="Demont Y" first="Yohann" last="Demont">Yohann Demont</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Corbet, Cyril" sort="Corbet, Cyril" uniqKey="Corbet C" first="Cyril" last="Corbet">Cyril Corbet</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Page, Adeline" sort="Page, Adeline" uniqKey="Page A" first="Adeline" last="Page">Adeline Page</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ataman Nal, Yasemin" sort="Ataman Nal, Yasemin" uniqKey="Ataman Nal Y" first="Yasemin" last="Ataman-Önal">Yasemin Ataman-Önal</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Choquet Kastylevsky, Genevieve" sort="Choquet Kastylevsky, Genevieve" uniqKey="Choquet Kastylevsky G" first="Genevieve" last="Choquet-Kastylevsky">Genevieve Choquet-Kastylevsky</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fliniaux, Ingrid" sort="Fliniaux, Ingrid" uniqKey="Fliniaux I" first="Ingrid" last="Fliniaux">Ingrid Fliniaux</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Le Bourhis, Xuefen" sort="Le Bourhis, Xuefen" uniqKey="Le Bourhis X" first="Xuefen" last="Le Bourhis">Xuefen Le Bourhis</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Toillon, Robert Alain" sort="Toillon, Robert Alain" uniqKey="Toillon R" first="Robert-Alain" last="Toillon">Robert-Alain Toillon</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bradshaw, Ralph A" sort="Bradshaw, Ralph A" uniqKey="Bradshaw R" first="Ralph A." last="Bradshaw">Ralph A. Bradshaw</name>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hondermarck, Hubert" sort="Hondermarck, Hubert" uniqKey="Hondermarck H" first="Hubert" last="Hondermarck">Hubert Hondermarck</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22128158</idno>
<idno type="pmc">3265873</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265873</idno>
<idno type="RBID">PMC:3265873</idno>
<idno type="doi">10.1074/jbc.M110.211714</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">001916</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001916</idno>
<idno type="wicri:Area/Pmc/Curation">001775</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001775</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002198</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002198</idno>
<idno type="wicri:Area/Ncbi/Merge">000C45</idno>
<idno type="wicri:Area/Ncbi/Curation">000C45</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000C45</idno>
<idno type="wicri:doubleKey">0021-9258:2011:Demont Y:pro:nerve:growth</idno>
<idno type="wicri:Area/Main/Merge">006398</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein<xref ref-type="fn" rid="FN1">*</xref>
<xref ref-type="fn" rid="FN2"><sup><inline-graphic xlink:href="sbox.jpg"></inline-graphic>
</sup>
</xref>
</title>
<author><name sortKey="Demont, Yohann" sort="Demont, Yohann" uniqKey="Demont Y" first="Yohann" last="Demont">Yohann Demont</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Corbet, Cyril" sort="Corbet, Cyril" uniqKey="Corbet C" first="Cyril" last="Corbet">Cyril Corbet</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Page, Adeline" sort="Page, Adeline" uniqKey="Page A" first="Adeline" last="Page">Adeline Page</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ataman Nal, Yasemin" sort="Ataman Nal, Yasemin" uniqKey="Ataman Nal Y" first="Yasemin" last="Ataman-Önal">Yasemin Ataman-Önal</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Choquet Kastylevsky, Genevieve" sort="Choquet Kastylevsky, Genevieve" uniqKey="Choquet Kastylevsky G" first="Genevieve" last="Choquet-Kastylevsky">Genevieve Choquet-Kastylevsky</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fliniaux, Ingrid" sort="Fliniaux, Ingrid" uniqKey="Fliniaux I" first="Ingrid" last="Fliniaux">Ingrid Fliniaux</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Le Bourhis, Xuefen" sort="Le Bourhis, Xuefen" uniqKey="Le Bourhis X" first="Xuefen" last="Le Bourhis">Xuefen Le Bourhis</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Toillon, Robert Alain" sort="Toillon, Robert Alain" uniqKey="Toillon R" first="Robert-Alain" last="Toillon">Robert-Alain Toillon</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bradshaw, Ralph A" sort="Bradshaw, Ralph A" uniqKey="Bradshaw R" first="Ralph A." last="Bradshaw">Ralph A. Bradshaw</name>
<affiliation><nlm:aff id="aff4"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hondermarck, Hubert" sort="Hondermarck, Hubert" uniqKey="Hondermarck H" first="Hubert" last="Hondermarck">Hubert Hondermarck</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of Biological Chemistry</title>
<idno type="ISSN">0021-9258</idno>
<idno type="eISSN">1083-351X</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The precursor of nerve growth factor (proNGF) has been described as a biologically active polypeptide able to induce apoptosis in neuronal cells, via the neurotrophin receptor p75<sup>NTR</sup>
and the sortilin receptor. Herein, it is shown that proNGF is produced and secreted by breast cancer cells, stimulating their invasion. Using Western blotting and mass spectrometry, proNGF was detected in a panel of breast cancer cells as well as in their conditioned media. Immunohistochemical analysis indicated an overproduction of proNGF in breast tumors, when compared with benign and normal breast biopsies, and a relationship to lymph node invasion in ductal carcinomas. Interestingly, siRNA against proNGF induced a decrease of breast cancer cell invasion that was restored by the addition of non-cleavable proNGF. The activation of TrkA, Akt, and Src, but not the MAP kinases, was observed. In addition, the proNGF invasive effect was inhibited by the Trk pharmacological inhibitor K252a, a kinase-dead TrkA, and siRNA against TrkA sortilin, neurotensin, whereas siRNA against p75<sup>NTR</sup>
and the MAP kinase inhibitor PD98059 had no impact. These data reveal the existence of an autocrine loop stimulated by proNGF and mediated by TrkA and sortilin, with the activation of Akt and Src, for the stimulation of breast cancer cell invasion.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006398 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 006398 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Main |étape= Merge |type= RBID |clé= PMC:3265873 |texte= Pro-nerve Growth Factor Induces Autocrine Stimulation of Breast Cancer Cell Invasion through Tropomyosin-related Kinase A (TrkA) and Sortilin Protein* }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:22128158" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |