AustralieFrV1, Main, Exploration, bibRecordById, pubmed:26731464

Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media.

Identifieur interne : 001490 ( Main/Exploration ); précédent : 001489; suivant : 001491

Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media.

Auteurs : Bochong Tang [République populaire de Chine] ; Jiaojiao Wang [République populaire de Chine] ; James A. Hutchison [France] ; Lei Ma [République populaire de Chine] ; Ning Zhang [République populaire de Chine] ; Hua Guo [République populaire de Chine] ; Zhongbo Hu [République populaire de Chine] ; Min Li [République populaire de Chine] ; Yuliang Zhao [République populaire de Chine]

Source :

ACS nano [ 1936-086X ] ; 2016.

RBID : pubmed:26731464

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies (chemistry), Biomarkers, Tumor (blood), Carcinoma, Hepatocellular (blood), Carcinoma, Hepatocellular (diagnosis), Cattle, Dimethylpolysiloxanes (chemistry), Early Diagnosis, Glypicans (blood), Humans, Immunoassay, Liver Neoplasms (blood), Liver Neoplasms (diagnosis), Metal Nanoparticles (chemistry), Metal Nanoparticles (ultrastructure), Nylons (chemistry), Printing (methods), Sensitivity and Specificity, Silver (chemistry), Spectrum Analysis, Raman (instrumentation), alpha-Fetoproteins (analysis).
MESH :
- chemical , analysis : alpha-Fetoproteins.
- chemical , blood : Biomarkers, Tumor, Glypicans.
- chemical , chemistry : Antibodies, Dimethylpolysiloxanes, Nylons, Silver.
- blood : Carcinoma, Hepatocellular, Liver Neoplasms.
- chemistry : Metal Nanoparticles.
- diagnosis : Carcinoma, Hepatocellular, Liver Neoplasms.
- instrumentation : Spectrum Analysis, Raman.
- methods : Printing.
- ultrastructure : Metal Nanoparticles.
- Animals, Cattle, Early Diagnosis, Humans, Immunoassay, Sensitivity and Specificity.

Abstract

Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.

DOI: 10.1021/acsnano.5b06007
PubMed: 26731464

Affiliations:

Le document en format XML

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<term>Carcinoma, Hepatocellular (blood)</term>
<term>Carcinoma, Hepatocellular (diagnosis)</term>
<term>Cattle</term>
<term>Dimethylpolysiloxanes (chemistry)</term>
<term>Early Diagnosis</term>
<term>Glypicans (blood)</term>
<term>Humans</term>
<term>Immunoassay</term>
<term>Liver Neoplasms (blood)</term>
<term>Liver Neoplasms (diagnosis)</term>
<term>Metal Nanoparticles (chemistry)</term>
<term>Metal Nanoparticles (ultrastructure)</term>
<term>Nylons (chemistry)</term>
<term>Printing (methods)</term>
<term>Sensitivity and Specificity</term>
<term>Silver (chemistry)</term>
<term>Spectrum Analysis, Raman (instrumentation)</term>
<term>alpha-Fetoproteins (analysis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alphafoetoprotéines (analyse)</term>
<term>Analyse spectrale Raman (instrumentation)</term>
<term>Animaux</term>
<term>Anticorps ()</term>
<term>Argent ()</term>
<term>Bovins</term>
<term>Carcinome hépatocellulaire (diagnostic)</term>
<term>Carcinome hépatocellulaire (sang)</term>
<term>Diagnostic précoce</term>
<term>Dosage immunologique</term>
<term>Glypicanes (sang)</term>
<term>Humains</term>
<term>Impression (typographie) ()</term>
<term>Marqueurs biologiques tumoraux (sang)</term>
<term>Nanoparticules métalliques ()</term>
<term>Nanoparticules métalliques (ultrastructure)</term>
<term>Nylons ()</term>
<term>Polydiméthylsiloxanes ()</term>
<term>Sensibilité et spécificité</term>
<term>Tumeurs du foie (diagnostic)</term>
<term>Tumeurs du foie (sang)</term>
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<term>Glypicans</term>
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<term>Dimethylpolysiloxanes</term>
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<term>Silver</term>
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</keywords>
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<term>Liver Neoplasms</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Carcinoma, Hepatocellular</term>
<term>Liver Neoplasms</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Carcinome hépatocellulaire</term>
<term>Tumeurs du foie</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Spectrum Analysis, Raman</term>
</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Analyse spectrale Raman</term>
<term>Carcinome hépatocellulaire</term>
<term>Glypicanes</term>
<term>Marqueurs biologiques tumoraux</term>
<term>Tumeurs du foie</term>
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</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cattle</term>
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<term>Humans</term>
<term>Immunoassay</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps</term>
<term>Argent</term>
<term>Bovins</term>
<term>Diagnostic précoce</term>
<term>Dosage immunologique</term>
<term>Humains</term>
<term>Impression (typographie)</term>
<term>Nanoparticules métalliques</term>
<term>Nylons</term>
<term>Polydiméthylsiloxanes</term>
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<front><div type="abstract" xml:lang="en">Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.</div>
</front>
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<name sortKey="Ma, Lei" sort="Ma, Lei" uniqKey="Ma L" first="Lei" last="Ma">Lei Ma</name>
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<name sortKey="Zhang, Ning" sort="Zhang, Ning" uniqKey="Zhang N" first="Ning" last="Zhang">Ning Zhang</name>
<name sortKey="Zhao, Yuliang" sort="Zhao, Yuliang" uniqKey="Zhao Y" first="Yuliang" last="Zhao">Yuliang Zhao</name>
</country>
<country name="France"><region name="Grand Est"><name sortKey="Hutchison, James A" sort="Hutchison, James A" uniqKey="Hutchison J" first="James A" last="Hutchison">James A. Hutchison</name>
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Serveur d'exploration sur les relations entre la France et l'Australie

Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media.

Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media.

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	Serveur d'exploration sur les relations entre la France et l'Australie
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