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Use of primary keratinocyte cultures from plucked human hairs for analysis of gap junctional intercellular communication

Identifieur interne : 00DC28 ( Main/Exploration ); précédent : 00DC27; suivant : 00DC29

Use of primary keratinocyte cultures from plucked human hairs for analysis of gap junctional intercellular communication

Auteurs : S. H. H. Swierenga [Canada] ; J. Fitzgerald [France, Australie] ; H. Yamasaki [France] ; C. Piccoli [France] ; M. Goldberg [Canada]

Source :

Toxicology in Vitro [ 0887-2333 ] ; 1991.

RBID : ISTEX:5F3A9889D5436D579883EB8EB9CA1E8DC78D3007

English descriptors

Abstract

Abstract: Hair follicles are a convenient source of human keratinocytes that are easily derived by non-invasive means. In order to test whether such cells could be used for gap junctional intercellular communication (GJIC) studies for the assessment of tumour-promoting activity of environmental chemicals, primary cultures were initiated from plucked hairs of various donors. GJIC measurements were performed when colonies reached a size of 5 mm in diameter, by microinjection of the fluorescent dye Lucifer Yellow CH into single cells at the colony periphery and scoring dye transfer to surrounding cells. The GJIC of cells that had begun to differentiate (appearance of cornified envelopes) can be quantitated more easily by switching cells to a low calcium (0.05-mm) medium. The tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibited GJIC in a concentration- and time-dependent manner, resembling the TPA response of mouse primary epidermal keratinocytes. Thus, hair follicle cultures can be readily used for GJIC studies and should be appropriate for other in vitro assays requiring attached cells.

Url:

https://api.istex.fr/document/5F3A9889D5436D579883EB8EB9CA1E8DC78D3007/fulltext/pdf

DOI: 10.1016/0887-2333(91)90063-J

Affiliations:

Australie, Canada, France

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Le document en format XML

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<author><name sortKey="Yamasaki, H" sort="Yamasaki, H" uniqKey="Yamasaki H" first="H." last="Yamasaki">H. Yamasaki</name>
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<author><name sortKey="Piccoli, C" sort="Piccoli, C" uniqKey="Piccoli C" first="C." last="Piccoli">C. Piccoli</name>
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<term>Cancer research</term>
<term>Carcinogenesis</term>
<term>Cell communication</term>
<term>Cell density</term>
<term>Chinese hamster</term>
<term>Colony morphology</term>
<term>Colony periphery</term>
<term>Epidermal</term>
<term>Epithelial cells</term>
<term>Fluorescence photographs</term>
<term>Follicle</term>
<term>Gjic</term>
<term>Gjic inhibition</term>
<term>Gjic studies</term>
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<term>Human cells</term>
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<term>Human keratinocytes</term>
<term>Injection sites</term>
<term>Intercellular</term>
<term>Intercellular communication</term>
<term>Intercellular junctions</term>
<term>Interexperimental variation</term>
<term>Intrinsic gjic</term>
<term>Investigative dermatology</term>
<term>Junctional</term>
<term>Junctional intercellular communication</term>
<term>Mek</term>
<term>Mouse cells</term>
<term>Phase contrast</term>
<term>Plenum press</term>
<term>Polychlorinated biphenyl congeners</term>
<term>Promoter</term>
<term>Recent advances</term>
<term>Same field</term>
<term>Sigma</term>
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<front><div type="abstract" xml:lang="en">Abstract: Hair follicles are a convenient source of human keratinocytes that are easily derived by non-invasive means. In order to test whether such cells could be used for gap junctional intercellular communication (GJIC) studies for the assessment of tumour-promoting activity of environmental chemicals, primary cultures were initiated from plucked hairs of various donors. GJIC measurements were performed when colonies reached a size of 5 mm in diameter, by microinjection of the fluorescent dye Lucifer Yellow CH into single cells at the colony periphery and scoring dye transfer to surrounding cells. The GJIC of cells that had begun to differentiate (appearance of cornified envelopes) can be quantitated more easily by switching cells to a low calcium (0.05-mm) medium. The tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibited GJIC in a concentration- and time-dependent manner, resembling the TPA response of mouse primary epidermal keratinocytes. Thus, hair follicle cultures can be readily used for GJIC studies and should be appropriate for other in vitro assays requiring attached cells.</div>
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Use of primary keratinocyte cultures from plucked human hairs for analysis of gap junctional intercellular communication

Use of primary keratinocyte cultures from plucked human hairs for analysis of gap junctional intercellular communication

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri