AustralieFrV1, Main, Exploration, bibRecord, 00D209

The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains

Identifieur interne : 00D209 ( Main/Exploration ); précédent : 00D208; suivant : 00D210

The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains

Auteurs : Tatiana Vallaeys [France] ; Nicholas C. Mcclure [Australie] ; Bruce Clare [Australie] ; Peter Murphy [Australie]

Source :

Biotechnology Letters [ 0141-5492 ] ; 1996-09-01.

RBID : ISTEX:636EF10FEE38689EB7ABB636A9E42AF1663CBE6E

Descripteurs français

Pascal (Inist)
- Agrobacterium tumefaciens, Catechol 2,3-dioxygenase, Détection, Expression génique, Gène hybride, Gène indicateur, Gène tdnC, Induction, Marqueur biologique, Microorganisme recombinant, Promoteur transcription, Sol, Survie, Vecteur.
Wicri :
- topic : Biotechnologie.

English descriptors

KwdEn :
- Agar plates, Agrobacterium, Agrobacterium strain, Agrobacterium strains, Agrobacterium tumefaciens, Bamhi, Bamhi cassette, Bamhi site, Biological marker, Biotechnology, Broad host range vector, Cassette, Catabolic, Catabolic plasmid ptdn1, Catechol, Catechol 2,3-dioxygenase, Codon, Delivery system, Detection, Elli, Ellis medium, Environmental microbiology, Gene, Gene expression, Host strain, Hybrid gene, Inducible, Inducible marker system, Inducible promoter, Induction, Lintig, Marker, Marker gene, Marker system, Mcclure, Microbiology, Nopaline, Plant roots, Plasmid, Plasmid ptvn1, Plasmid ptvnc2, Promoter, Pseudomonas, Ptvnc2, Rapid detection, Recombinant microorganism, Regulatory gene, Reporter gene, Single bamhi site, Soils, Survival, Tdnc, Tdnc gene, Transcription promoter, Transposon, Tumefaciens, Tumefaciens strains, Vector, Winstanley.
Teeft :
- Agar plates, Agrobacterium, Agrobacterium strain, Agrobacterium strains, Agrobacterium tumefaciens, Bamhi, Bamhi cassette, Bamhi site, Biotechnology, Broad host range vector, Cassette, Catabolic, Catabolic plasmid ptdn1, Catechol, Codon, Delivery system, Elli, Ellis medium, Environmental microbiology, Gene, Host strain, Inducible, Inducible marker system, Inducible promoter, Lintig, Marker, Marker gene, Marker system, Mcclure, Microbiology, Nopaline, Plant roots, Plasmid, Plasmid ptvn1, Plasmid ptvnc2, Promoter, Pseudomonas, Ptvnc2, Rapid detection, Regulatory gene, Single bamhi site, Tdnc, Tdnc gene, Transposon, Tumefaciens, Tumefaciens strains, Winstanley.

Abstract

Summary: An inducible marker system suitable for Agrobacterium tumefaciens was constructed to enable detection and enumeration of specific bacterial cells introduced into soil. A BamHI cassette carrying the catechol 2,3-dipxygenase (C230) gene, tdnC, fused to the nopaline-inducible Agrobacterium promoter Pi 2[noc] was constructed. This cassette was introduced into the broad host range vector pDSK5019 resulting in plasmid pTVNC2. Inducible C230 activity was observed in an A. tumefaciens strain carrying the plasmid pTVNC2 when nopaline was present. Colonies of bacteria tagged with the system could easily be identified by spraying agar plates containing nopaline with catechol, which is converted to the bright yellow compound 2-hydroxymuconic semialdehyde. The inducible tdnC cassette has also been introduced into the BamHI site of the transposon Tn5 carried by the pSUP1011 suicide vector which can be used as a delivery system for the stable introduction of the inducible marker into the chromosome of target cells.

Url:

https://api.istex.fr/document/636EF10FEE38689EB7ABB636A9E42AF1663CBE6E/fulltext/pdf

DOI: 10.1007/BF00129719

Affiliations:

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<front><div type="abstract" xml:lang="en">Summary: An inducible marker system suitable for Agrobacterium tumefaciens was constructed to enable detection and enumeration of specific bacterial cells introduced into soil. A BamHI cassette carrying the catechol 2,3-dipxygenase (C230) gene, tdnC, fused to the nopaline-inducible Agrobacterium promoter Pi 2[noc] was constructed. This cassette was introduced into the broad host range vector pDSK5019 resulting in plasmid pTVNC2. Inducible C230 activity was observed in an A. tumefaciens strain carrying the plasmid pTVNC2 when nopaline was present. Colonies of bacteria tagged with the system could easily be identified by spraying agar plates containing nopaline with catechol, which is converted to the bright yellow compound 2-hydroxymuconic semialdehyde. The inducible tdnC cassette has also been introduced into the BamHI site of the transposon Tn5 carried by the pSUP1011 suicide vector which can be used as a delivery system for the stable introduction of the inducible marker into the chromosome of target cells.</div>
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The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains

The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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Pour manipuler ce document sous Unix (Dilib)

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