The WHO multicenter study on Listeria monocytogenes subtyping: Random amplification of polymorphic DNA (RAPD)
Identifieur interne : 00D136 ( Main/Exploration ); précédent : 00D135; suivant : 00D137The WHO multicenter study on Listeria monocytogenes subtyping: Random amplification of polymorphic DNA (RAPD)
Auteurs : K. Wernars [Pays-Bas] ; P. Boerlin [Suisse] ; A. Audurier [France] ; E. G. Russell [Australie] ; G. D. W. Curtis [Royaume-Uni] ; L. Herman [Belgique] ; N. Van Der Mee-Marquet [France]Source :
- International Journal of Food Microbiology [ 0168-1605 ] ; 1996.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Protocole.
English descriptors
- KwdEn :
- Amplification, Average correlation, Average reproducibility, Cell concentration, Different laboratories, Different participants, Different primers, Different types, Electrophoresis conditions, Epidemiological, Epidemiological groups, Epidemiological typing, Experimental procedure, Food microbiology, Initial cultures, Listeria monocytogenes, Method, Microbial, Microbiology, Molecular epidemiology, Multicenter study, Other primers, Participant, Pathogenic, Performance evaluation, Polymerase chain reaction, Polymorphic, Primer, Protocol, Random amplification, Random amplified polymorphic DNA, Rapd, Rapd analysis, Rapd profiles, Rapd reaction mixture, Rapd results, Reaction mixture, Reproducibility, Reproducibility problems, Reproducible results, Single colony, Standard protocol, Standard rapd protocol, Strain, Subtyping methods, Test strains, Typing, Unrelated strains, Various locations, WHO, Wernars.
- Teeft :
- Amplification, Average correlation, Average reproducibility, Cell concentration, Different laboratories, Different participants, Different primers, Different types, Electrophoresis conditions, Epidemiological, Epidemiological groups, Epidemiological typing, Experimental procedure, Food microbiology, Initial cultures, Microbial, Microbiology, Multicenter study, Other primers, Participant, Polymorphic, Primer, Protocol, Random amplification, Rapd, Rapd analysis, Rapd profiles, Rapd reaction mixture, Rapd results, Reaction mixture, Reproducibility, Reproducibility problems, Reproducible results, Single colony, Standard protocol, Standard rapd protocol, Subtyping methods, Test strains, Unrelated strains, Various locations, Wernars.
Abstract
Abstract: As part of a WHO multicenter study on Listeria monocytogenes subtyping methods the random amplification of polymorphic DNA (RAPD)-technique was evaluated. Six participants were asked to use a standard protocol to analyse a set of 80 L. monocytogenes strains. This set contained 22 groups of epidemiologically linked isolates and 11 pairs of duplicate strains. Using three different 10-mer primers, the median reproducibility of the RAPD-results obtained by the six participants was 86.5% (range 0–100%). Failure in reproducibility was mainly due to results obtained with one particular primer. The number of epidemiological groups found to be homogeneous varied from 1–22 (median 16). However, for some groups an inhomogeneity was found by the majority of participants. The overall correlation between the results from the different participants ranged from 32 to 85%.
Url:
DOI: 10.1016/S0168-1605(96)01146-4
Affiliations:
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Van Der Mee-Marquet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Centre Hospitalier Universitaire, Faculté de Médecine, Laboratoire de Bactériologie et d'Hygiéne Hospitalière, F-37044 Tours Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">International Journal of Food Microbiology</title><title level="j" type="abbrev">FOOD</title><idno type="ISSN">0168-1605</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="325">325</biblScope><biblScope unit="page" to="341">341</biblScope></imprint><idno type="ISSN">0168-1605</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-1605</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplification</term><term>Average correlation</term><term>Average reproducibility</term><term>Cell concentration</term><term>Different laboratories</term><term>Different participants</term><term>Different primers</term><term>Different types</term><term>Electrophoresis conditions</term><term>Epidemiological</term><term>Epidemiological groups</term><term>Epidemiological typing</term><term>Experimental procedure</term><term>Food microbiology</term><term>Initial cultures</term><term>Listeria monocytogenes</term><term>Method</term><term>Microbial</term><term>Microbiology</term><term>Molecular epidemiology</term><term>Multicenter study</term><term>Other primers</term><term>Participant</term><term>Pathogenic</term><term>Performance evaluation</term><term>Polymerase chain reaction</term><term>Polymorphic</term><term>Primer</term><term>Protocol</term><term>Random amplification</term><term>Random amplified polymorphic DNA</term><term>Rapd</term><term>Rapd analysis</term><term>Rapd profiles</term><term>Rapd reaction mixture</term><term>Rapd results</term><term>Reaction mixture</term><term>Reproducibility</term><term>Reproducibility problems</term><term>Reproducible results</term><term>Single colony</term><term>Standard protocol</term><term>Standard rapd protocol</term><term>Strain</term><term>Subtyping methods</term><term>Test strains</term><term>Typing</term><term>Unrelated strains</term><term>Various locations</term><term>WHO</term><term>Wernars</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Epidémiologie moléculaire</term><term>Etude multicentrique</term><term>Evaluation performance</term><term>Listeria monocytogenes</term><term>Marqueur RAPD</term><term>Méthode</term><term>OMS</term><term>Pathogène</term><term>Reproductibilité</term><term>Réaction chaîne polymérase</term><term>Souche</term><term>Typage</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Amplification</term><term>Average correlation</term><term>Average reproducibility</term><term>Cell concentration</term><term>Different laboratories</term><term>Different participants</term><term>Different primers</term><term>Different types</term><term>Electrophoresis conditions</term><term>Epidemiological</term><term>Epidemiological groups</term><term>Epidemiological typing</term><term>Experimental procedure</term><term>Food microbiology</term><term>Initial cultures</term><term>Microbial</term><term>Microbiology</term><term>Multicenter study</term><term>Other primers</term><term>Participant</term><term>Polymorphic</term><term>Primer</term><term>Protocol</term><term>Random amplification</term><term>Rapd</term><term>Rapd analysis</term><term>Rapd profiles</term><term>Rapd reaction mixture</term><term>Rapd results</term><term>Reaction mixture</term><term>Reproducibility</term><term>Reproducibility problems</term><term>Reproducible results</term><term>Single colony</term><term>Standard protocol</term><term>Standard rapd protocol</term><term>Subtyping methods</term><term>Test strains</term><term>Unrelated strains</term><term>Various locations</term><term>Wernars</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Protocole</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: As part of a WHO multicenter study on Listeria monocytogenes subtyping methods the random amplification of polymorphic DNA (RAPD)-technique was evaluated. Six participants were asked to use a standard protocol to analyse a set of 80 L. monocytogenes strains. This set contained 22 groups of epidemiologically linked isolates and 11 pairs of duplicate strains. Using three different 10-mer primers, the median reproducibility of the RAPD-results obtained by the six participants was 86.5% (range 0–100%). Failure in reproducibility was mainly due to results obtained with one particular primer. The number of epidemiological groups found to be homogeneous varied from 1–22 (median 16). However, for some groups an inhomogeneity was found by the majority of participants. The overall correlation between the results from the different participants ranged from 32 to 85%.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Belgique</li><li>France</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>Suisse</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li></region><settlement><li>Tours</li></settlement></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Wernars, K" sort="Wernars, K" uniqKey="Wernars K" first="K." last="Wernars">K. Wernars</name></noRegion><name sortKey="Wernars, K" sort="Wernars, K" uniqKey="Wernars K" first="K." last="Wernars">K. Wernars</name></country><country name="Suisse"><noRegion><name sortKey="Boerlin, P" sort="Boerlin, P" uniqKey="Boerlin P" first="P." last="Boerlin">P. Boerlin</name></noRegion></country><country name="France"><region name="Centre-Val de Loire"><name sortKey="Audurier, A" sort="Audurier, A" uniqKey="Audurier A" first="A." last="Audurier">A. Audurier</name></region><name sortKey="Van Der Mee Marquet, N" sort="Van Der Mee Marquet, N" uniqKey="Van Der Mee Marquet N" first="N." last="Van Der Mee-Marquet">N. Van Der Mee-Marquet</name></country><country name="Australie"><noRegion><name sortKey="Russell, E G" sort="Russell, E G" uniqKey="Russell E" first="E. G." last="Russell">E. G. Russell</name></noRegion></country><country name="Royaume-Uni"><noRegion><name sortKey="Curtis, G D W" sort="Curtis, G D W" uniqKey="Curtis G" first="G. D. W." last="Curtis">G. D. W. Curtis</name></noRegion></country><country name="Belgique"><noRegion><name sortKey="Herman, L" sort="Herman, L" uniqKey="Herman L" first="L." last="Herman">L. Herman</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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