Analysis of a 14-kb fragment containing a putative cell wall gene and a candidate for the ARA1 , arabinose kinase, gene from chromosome IV of Arabidopsis thaliana
Identifieur interne : 00C932 ( Main/Exploration ); précédent : 00C931; suivant : 00C933Analysis of a 14-kb fragment containing a putative cell wall gene and a candidate for the ARA1 , arabinose kinase, gene from chromosome IV of Arabidopsis thaliana
Auteurs : Isabelle Gy [France] ; Sébastien Aubourg [France] ; Sarah Sherson [Australie] ; Christopher S. Cobbett [Australie] ; Angélique Cheron [France] ; Martin Kreis [France] ; Alain Lecharny [France]Source :
- Gene [ 0378-1119 ] ; 1998.
Descripteurs français
- Wicri :
- topic : Base de données.
English descriptors
- KwdEn :
- Accession number, Amino, Amino acids, Ara1, Ara1 locus, Arabidopsis, Arabidopsis thaliana, Arabinose, Arabinose kinase, Arabinose kinase activity, Atisa1, Atisa1 gene, Atisa1 probe, Atisa1 protein, Atseb1, Atseb1 cdna, Atseb1 probe, Atseb2, Atseb2 gene, Biol, Cdna, Cdna clone, Cdna libraries, Chromosome, Clone, Clone number, Cobbett, Codon, Codon usage, Cosmid, Database, Exon, Fragment, Gal1, Galactokinase, Galactokinase family, Galactokinase signature, Gene, Genefinder, Genes atseb1, Genome, Genomic, Hybridization, Intron, Kinase, Kreis, Locus, Mrna, Nucleic, Nucleic acids, Nucleotide, Physiol, Plant physiol, Plasma membrane, Primer, Putative, Recombinant phage, Saccharomyces cerevisiae, Schematic representation, Sequence analysis, Sequencing, Silique, Silique cdna library, Southern blot hybridization, Stage lane, Thaliana, Untranslated regions.
- Teeft :
- Accession number, Amino, Amino acids, Ara1, Ara1 locus, Arabidopsis, Arabidopsis thaliana, Arabinose, Arabinose kinase, Arabinose kinase activity, Atisa1, Atisa1 gene, Atisa1 probe, Atisa1 protein, Atseb1, Atseb1 cdna, Atseb1 probe, Atseb2, Atseb2 gene, Biol, Cdna, Cdna clone, Cdna libraries, Chromosome, Clone, Clone number, Cobbett, Codon, Codon usage, Cosmid, Database, Exon, Fragment, Gal1, Galactokinase, Galactokinase family, Galactokinase signature, Gene, Genefinder, Genes atseb1, Genome, Genomic, Hybridization, Intron, Kinase, Kreis, Locus, Mrna, Nucleic, Nucleic acids, Nucleotide, Physiol, Plant physiol, Plasma membrane, Primer, Putative, Recombinant phage, Saccharomyces cerevisiae, Schematic representation, Sequence analysis, Sequencing, Silique, Silique cdna library, Southern blot hybridization, Stage lane, Thaliana, Untranslated regions.
Abstract
Abstract: An Arabidopsis thaliana genomic DNA fragment of 14kb has been characterized in the framework of the E.S.S.A. programme. Computational and molecular approaches identified three novel gene sequences coding, respectively, for a protein of unknown function, a putative membrane-anchored cell wall protein and an arabinose kinase gene corresponding to the locus ARA1. The latter two genes named AtSEB1 and AtISA1 have been characterized in detail. They are very different in their organization, codon usage and level of expression. Homologues of AtSEB1 and AtISA1 have been identified. Sequence comparisons showed that the former genes contained a long 5′ extension coding for an N-terminal domain probably specifying subcellular localization. Cloning and sequencing of the cognate cDNA for the AtISA1 homologue in A. thaliana, named GAL1, indicate that it encodes for a galactokinase-like protein. Our results highlight the integrative outcome of a systematic sequencing project in which links between biochemically and genetically characterized mutants, ESTs and genomic sequence data are generated.
Url:
DOI: 10.1016/S0378-1119(98)00049-3
Affiliations:
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type="city">Orsay</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">209</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="201">201</biblScope><biblScope unit="page" to="210">210</biblScope></imprint><idno type="ISSN">0378-1119</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Accession number</term><term>Amino</term><term>Amino acids</term><term>Ara1</term><term>Ara1 locus</term><term>Arabidopsis</term><term>Arabidopsis thaliana</term><term>Arabinose</term><term>Arabinose kinase</term><term>Arabinose kinase activity</term><term>Atisa1</term><term>Atisa1 gene</term><term>Atisa1 probe</term><term>Atisa1 protein</term><term>Atseb1</term><term>Atseb1 cdna</term><term>Atseb1 probe</term><term>Atseb2</term><term>Atseb2 gene</term><term>Biol</term><term>Cdna</term><term>Cdna clone</term><term>Cdna libraries</term><term>Chromosome</term><term>Clone</term><term>Clone number</term><term>Cobbett</term><term>Codon</term><term>Codon usage</term><term>Cosmid</term><term>Database</term><term>Exon</term><term>Fragment</term><term>Gal1</term><term>Galactokinase</term><term>Galactokinase family</term><term>Galactokinase signature</term><term>Gene</term><term>Genefinder</term><term>Genes atseb1</term><term>Genome</term><term>Genomic</term><term>Hybridization</term><term>Intron</term><term>Kinase</term><term>Kreis</term><term>Locus</term><term>Mrna</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide</term><term>Physiol</term><term>Plant physiol</term><term>Plasma membrane</term><term>Primer</term><term>Putative</term><term>Recombinant phage</term><term>Saccharomyces cerevisiae</term><term>Schematic representation</term><term>Sequence analysis</term><term>Sequencing</term><term>Silique</term><term>Silique cdna library</term><term>Southern blot hybridization</term><term>Stage lane</term><term>Thaliana</term><term>Untranslated regions</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Accession number</term><term>Amino</term><term>Amino acids</term><term>Ara1</term><term>Ara1 locus</term><term>Arabidopsis</term><term>Arabidopsis thaliana</term><term>Arabinose</term><term>Arabinose kinase</term><term>Arabinose kinase activity</term><term>Atisa1</term><term>Atisa1 gene</term><term>Atisa1 probe</term><term>Atisa1 protein</term><term>Atseb1</term><term>Atseb1 cdna</term><term>Atseb1 probe</term><term>Atseb2</term><term>Atseb2 gene</term><term>Biol</term><term>Cdna</term><term>Cdna clone</term><term>Cdna libraries</term><term>Chromosome</term><term>Clone</term><term>Clone number</term><term>Cobbett</term><term>Codon</term><term>Codon usage</term><term>Cosmid</term><term>Database</term><term>Exon</term><term>Fragment</term><term>Gal1</term><term>Galactokinase</term><term>Galactokinase family</term><term>Galactokinase signature</term><term>Gene</term><term>Genefinder</term><term>Genes atseb1</term><term>Genome</term><term>Genomic</term><term>Hybridization</term><term>Intron</term><term>Kinase</term><term>Kreis</term><term>Locus</term><term>Mrna</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide</term><term>Physiol</term><term>Plant physiol</term><term>Plasma membrane</term><term>Primer</term><term>Putative</term><term>Recombinant phage</term><term>Saccharomyces cerevisiae</term><term>Schematic representation</term><term>Sequence analysis</term><term>Sequencing</term><term>Silique</term><term>Silique cdna library</term><term>Southern blot hybridization</term><term>Stage lane</term><term>Thaliana</term><term>Untranslated regions</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Base de données</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: An Arabidopsis thaliana genomic DNA fragment of 14kb has been characterized in the framework of the E.S.S.A. programme. Computational and molecular approaches identified three novel gene sequences coding, respectively, for a protein of unknown function, a putative membrane-anchored cell wall protein and an arabinose kinase gene corresponding to the locus ARA1. The latter two genes named AtSEB1 and AtISA1 have been characterized in detail. They are very different in their organization, codon usage and level of expression. Homologues of AtSEB1 and AtISA1 have been identified. Sequence comparisons showed that the former genes contained a long 5′ extension coding for an N-terminal domain probably specifying subcellular localization. Cloning and sequencing of the cognate cDNA for the AtISA1 homologue in A. thaliana, named GAL1, indicate that it encodes for a galactokinase-like protein. Our results highlight the integrative outcome of a systematic sequencing project in which links between biochemically and genetically characterized mutants, ESTs and genomic sequence data are generated.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Victoria (État)</li><li>Île-de-France</li></region><settlement><li>Melbourne</li><li>Orsay</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Gy, Isabelle" sort="Gy, Isabelle" uniqKey="Gy I" first="Isabelle" last="Gy">Isabelle Gy</name></noRegion><name sortKey="Aubourg, Sebastien" sort="Aubourg, Sebastien" uniqKey="Aubourg S" first="Sébastien" last="Aubourg">Sébastien Aubourg</name><name sortKey="Cheron, Angelique" sort="Cheron, Angelique" uniqKey="Cheron A" first="Angélique" last="Cheron">Angélique Cheron</name><name sortKey="Kreis, Martin" sort="Kreis, Martin" uniqKey="Kreis M" first="Martin" last="Kreis">Martin Kreis</name><name sortKey="Lecharny, Alain" sort="Lecharny, Alain" uniqKey="Lecharny A" first="Alain" last="Lecharny">Alain Lecharny</name></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Sherson, Sarah" sort="Sherson, Sarah" uniqKey="Sherson S" first="Sarah" last="Sherson">Sarah Sherson</name></region><name sortKey="Cobbett, Christopher S" sort="Cobbett, Christopher S" uniqKey="Cobbett C" first="Christopher S" last="Cobbett">Christopher S. 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