Microscale heterogeneity in the activity of coastal bacterioplankton communities
Identifieur interne : 00AB97 ( Main/Exploration ); précédent : 00AB96; suivant : 00AB98Microscale heterogeneity in the activity of coastal bacterioplankton communities
Auteurs : Justin R. Seymour [Australie] ; James G. Mitchell [Australie] ; Laurent Seuront [Australie, France]Source :
- Aquatic microbial ecology [ 0948-3055 ] ; 2004.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Milieu aquatique.
English descriptors
- KwdEn :
Abstract
Microscale sampling techniques and flow cytometry were employed to measure the distribution patterns of 8 subpopulations of bacteria separated according to variations in cell fluorescence and light scatter properties. Subpopulations of bacteria defined on the basis of these parameters have recently been shown to represent cells exhibiting dissimilar activity levels, and we therefore assume that the subpopulations of bacteria identified here represent metabolically diverse groups. Microscale distribution patterns of these subpopulations were measured at a resolution of 4.5 and 12 mm, within 2 dissimilar coastal habitats. A mean 2-fold change in the abundance of the total bacterial community across sample sets was observed. However, levels of spatial heterogeneity were consistently higher for the cytometrically defined subpopulations than total counts. In most samples, the population of bacteria exhibiting the highest levels of green fluorescence, or DNA content, and hence assumed to represent the most active bacteria in the community, also showed the highest levels of microscale spatial variability, with a maximum change in abundance of 14.5-fold observed across a distance of 9 mm. Where Zipf rank-size analysis was conducted, the microscale distributions of subpopulations differed significantly (p < 0.05) in 79% of cases, implying that bacterial communities are made up of physiologically distinct compartments, perhaps influenced by different microscale features of the environment. We suggest that these results provide the first evidence for the existence of microscale heterogeneity in the metabolic activity of aquatic bacterial communities.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 004F19
- to stream PascalFrancis, to step Curation: 001195
- to stream PascalFrancis, to step Checkpoint: 004A64
- to stream Main, to step Merge: 00B891
- to stream Main, to step Curation: 00AB97
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Microscale heterogeneity in the activity of coastal bacterioplankton communities</title><author><name sortKey="Seymour, Justin R" sort="Seymour, Justin R" uniqKey="Seymour J" first="Justin R." last="Seymour">Justin R. Seymour</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biological Sciences, Flinders University, PO Box 2100</s1><s2>Adelaide, South Australia 5001</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, South Australia 5001</wicri:noRegion></affiliation></author><author><name sortKey="Mitchell, James G" sort="Mitchell, James G" uniqKey="Mitchell J" first="James G." last="Mitchell">James G. Mitchell</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biological Sciences, Flinders University, PO Box 2100</s1><s2>Adelaide, South Australia 5001</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, South Australia 5001</wicri:noRegion></affiliation></author><author><name sortKey="Seuront, Laurent" sort="Seuront, Laurent" uniqKey="Seuront L" first="Laurent" last="Seuront">Laurent Seuront</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biological Sciences, Flinders University, PO Box 2100</s1><s2>Adelaide, South Australia 5001</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, South Australia 5001</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Ecosystem Complexity Research Group, Station Marine de Wimereux, CNRS-UMR 8013 ELICO, Université des Sciences et Technologies de Lille, 28 avenue Foch</s1><s2>62930 Wimereux</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>62930 Wimereux</wicri:noRegion><wicri:noRegion>28 avenue Foch</wicri:noRegion><wicri:noRegion>62930 Wimereux</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0253500</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0253500 INIST</idno><idno type="RBID">Pascal:04-0253500</idno><idno type="wicri:Area/PascalFrancis/Corpus">004F19</idno><idno type="wicri:Area/PascalFrancis/Curation">001195</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">004A64</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">004A64</idno><idno type="wicri:doubleKey">0948-3055:2004:Seymour J:microscale:heterogeneity:in</idno><idno type="wicri:Area/Main/Merge">00B891</idno><idno type="wicri:Area/Main/Curation">00AB97</idno><idno type="wicri:Area/Main/Exploration">00AB97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Microscale heterogeneity in the activity of coastal bacterioplankton communities</title><author><name sortKey="Seymour, Justin R" sort="Seymour, Justin R" uniqKey="Seymour J" first="Justin R." last="Seymour">Justin R. Seymour</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biological Sciences, Flinders University, PO Box 2100</s1><s2>Adelaide, South Australia 5001</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, South Australia 5001</wicri:noRegion></affiliation></author><author><name sortKey="Mitchell, James G" sort="Mitchell, James G" uniqKey="Mitchell J" first="James G." last="Mitchell">James G. Mitchell</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biological Sciences, Flinders University, PO Box 2100</s1><s2>Adelaide, South Australia 5001</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, South Australia 5001</wicri:noRegion></affiliation></author><author><name sortKey="Seuront, Laurent" sort="Seuront, Laurent" uniqKey="Seuront L" first="Laurent" last="Seuront">Laurent Seuront</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biological Sciences, Flinders University, PO Box 2100</s1><s2>Adelaide, South Australia 5001</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide, South Australia 5001</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Ecosystem Complexity Research Group, Station Marine de Wimereux, CNRS-UMR 8013 ELICO, Université des Sciences et Technologies de Lille, 28 avenue Foch</s1><s2>62930 Wimereux</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>62930 Wimereux</wicri:noRegion><wicri:noRegion>28 avenue Foch</wicri:noRegion><wicri:noRegion>62930 Wimereux</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Aquatic microbial ecology</title><title level="j" type="abbreviated">Aquat. microb. ecol.</title><idno type="ISSN">0948-3055</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Aquatic microbial ecology</title><title level="j" type="abbreviated">Aquat. microb. ecol.</title><idno type="ISSN">0948-3055</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aquatic environment</term><term>Bacteria</term><term>Bacteriosis</term><term>Ecology</term><term>Flow cytometry</term><term>Microbial community</term><term>Microbiology</term><term>Samplings</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Prélèvement</term><term>Cytométrie flux</term><term>Bactérie</term><term>Communauté microbienne</term><term>Milieu aquatique</term><term>Microbiologie</term><term>Ecologie</term><term>Bactériose</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Milieu aquatique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microscale sampling techniques and flow cytometry were employed to measure the distribution patterns of 8 subpopulations of bacteria separated according to variations in cell fluorescence and light scatter properties. Subpopulations of bacteria defined on the basis of these parameters have recently been shown to represent cells exhibiting dissimilar activity levels, and we therefore assume that the subpopulations of bacteria identified here represent metabolically diverse groups. Microscale distribution patterns of these subpopulations were measured at a resolution of 4.5 and 12 mm, within 2 dissimilar coastal habitats. A mean 2-fold change in the abundance of the total bacterial community across sample sets was observed. However, levels of spatial heterogeneity were consistently higher for the cytometrically defined subpopulations than total counts. In most samples, the population of bacteria exhibiting the highest levels of green fluorescence, or DNA content, and hence assumed to represent the most active bacteria in the community, also showed the highest levels of microscale spatial variability, with a maximum change in abundance of 14.5-fold observed across a distance of 9 mm. Where Zipf rank-size analysis was conducted, the microscale distributions of subpopulations differed significantly (p < 0.05) in 79% of cases, implying that bacterial communities are made up of physiologically distinct compartments, perhaps influenced by different microscale features of the environment. We suggest that these results provide the first evidence for the existence of microscale heterogeneity in the metabolic activity of aquatic bacterial communities.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Seymour, Justin R" sort="Seymour, Justin R" uniqKey="Seymour J" first="Justin R." last="Seymour">Justin R. Seymour</name></noRegion><name sortKey="Mitchell, James G" sort="Mitchell, James G" uniqKey="Mitchell J" first="James G." last="Mitchell">James G. Mitchell</name><name sortKey="Seuront, Laurent" sort="Seuront, Laurent" uniqKey="Seuront L" first="Laurent" last="Seuront">Laurent Seuront</name></country><country name="France"><noRegion><name sortKey="Seuront, Laurent" sort="Seuront, Laurent" uniqKey="Seuront L" first="Laurent" last="Seuront">Laurent Seuront</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00AB97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 00AB97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:04-0253500
|texte= Microscale heterogeneity in the activity of coastal bacterioplankton communities
}}
| This area was generated with Dilib version V0.6.33. |  |