Isolation and characterization of 16 microsatellite loci in the humbug damselfish, Dascyllus aruanus (family Pomacentridae)
Identifieur interne : 007E38 ( Main/Exploration ); précédent : 007E37; suivant : 007E39Isolation and characterization of 16 microsatellite loci in the humbug damselfish, Dascyllus aruanus (family Pomacentridae)
Auteurs : C. Fauvelot [France] ; C. Smith-Keune [Australie] ; D. R. Jerry [Australie] ; P. M. Buston [Espagne] ; S. Planes [France, Polynésie française]Source :
- Molecular Ecology Resources [ 1755-098X ] ; 2009-03.
Descripteurs français
English descriptors
- KwdEn :
- Allele, Annealing, Aruanus, Authors journal compilation, Beckman, Beckman coulter, Blackwell publishing, Carneus, Coulter, Damselfish, Dascyllus, Dascyllus aruanus, Dascyllus carneus, Dascyllus strasburgi, Dascyllus trimaculatus, Ecology, French polynesia, Genetic analysis system, Heterozygosity, Humbug damselfish, James cook university, Locus, Microsatellite, Microsatellite loci, Molecular ecology notes, Moorea, Null alleles, Perpignan, Perpignan cedex, Polymorphic, Polynesia, Pomacentridae, Primer, Strasburgi, Trimaculatus, Various annealing temperatures.
- Teeft :
- Allele, Annealing, Aruanus, Authors journal compilation, Beckman, Beckman coulter, Blackwell publishing, Carneus, Coulter, Damselfish, Dascyllus, Dascyllus aruanus, Dascyllus carneus, Dascyllus strasburgi, Dascyllus trimaculatus, Ecology, French polynesia, Genetic analysis system, Heterozygosity, Humbug damselfish, James cook university, Locus, Microsatellite, Microsatellite loci, Molecular ecology notes, Moorea, Null alleles, Perpignan, Perpignan cedex, Polymorphic, Polynesia, Pomacentridae, Primer, Strasburgi, Trimaculatus, Various annealing temperatures.
Abstract
Here we report on 16 microsatellite loci designed for the damselfish Dascyllus aruanus. All loci were tested on 98 individuals and were polymorphic (seven to 35 alleles). Expected heterozygosity ranged from 0.705 to 0.942. Six loci showed Hardy‐Weinberg disequilibrium due to the occurrence of null alleles. Cross‐species amplifications conducted within the genus Dascyllus (D. carneus, D. strasburgi, D. trimaculatus) lead to polymorphic fragments in 32 out of 48 tests. These 16 loci will enable future research into the behavioural ecology and population ecology of Dascyllus aruanus throughout the Indo‐Pacific.
Url:
DOI: 10.1111/j.1755-0998.2008.02500.x
Affiliations:
- Australie, Espagne, France, Polynésie française
- Languedoc-Roussillon, Occitanie (région administrative)
- Perpignan
Links toward previous steps (curation, corpus...)
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- to stream Istex, to step Curation: 001915
- to stream Istex, to step Checkpoint: 000E75
- to stream Main, to step Merge: 008533
- to stream Main, to step Curation: 007E38
Le document en format XML
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<front><div type="abstract" xml:lang="en">Here we report on 16 microsatellite loci designed for the damselfish Dascyllus aruanus. All loci were tested on 98 individuals and were polymorphic (seven to 35 alleles). Expected heterozygosity ranged from 0.705 to 0.942. Six loci showed Hardy‐Weinberg disequilibrium due to the occurrence of null alleles. Cross‐species amplifications conducted within the genus Dascyllus (D. carneus, D. strasburgi, D. trimaculatus) lead to polymorphic fragments in 32 out of 48 tests. These 16 loci will enable future research into the behavioural ecology and population ecology of Dascyllus aruanus throughout the Indo‐Pacific.</div>
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