Applying antibiotic selection markers for nematode genetics.
Identifieur interne : 003B94 ( Main/Exploration ); précédent : 003B93; suivant : 003B95Applying antibiotic selection markers for nematode genetics.
Auteurs : Eric Cornes [France] ; Cécile A L. Quéré [France] ; Rosina Giordano-Santini [Australie] ; Denis Dupuy [France]Source :
- Methods (San Diego, Calif.) [ 1095-9130 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Caenorhabditis elegans.
- pharmacologie : Antibactériens.
- Animaux, Caenorhabditis elegans, Marqueurs biologiques, Mutation, Sélection génétique, Techniques de transfert de gènes, Transformation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Anti-Bacterial Agents.
- drug effects : Caenorhabditis elegans.
- genetics : Caenorhabditis elegans.
- Animals, Biomarkers, Gene Transfer Techniques, Mutation, Selection, Genetic, Transformation, Genetic.
Abstract
Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.
DOI: 10.1016/j.ymeth.2014.04.016
PubMed: 24821108
Affiliations:
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Le document en format XML
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Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. 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Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France. Electronic address: d.dupuy@iecb.u-bordeaux.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux</wicri:regionArea><wicri:noRegion>33000 Bordeaux</wicri:noRegion><wicri:noRegion>33000 Bordeaux</wicri:noRegion></affiliation></author></analytic><series><title level="j">Methods (San Diego, Calif.)</title><idno type="eISSN">1095-9130</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Biomarkers</term><term>Caenorhabditis elegans (drug effects)</term><term>Caenorhabditis elegans (genetics)</term><term>Gene Transfer Techniques</term><term>Mutation</term><term>Selection, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antibactériens (pharmacologie)</term><term>Caenorhabditis elegans ()</term><term>Caenorhabditis elegans (génétique)</term><term>Marqueurs biologiques</term><term>Mutation</term><term>Sélection génétique</term><term>Techniques de transfert de gènes</term><term>Transformation génétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Caenorhabditis elegans</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Caenorhabditis elegans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Caenorhabditis elegans</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biomarkers</term><term>Gene Transfer Techniques</term><term>Mutation</term><term>Selection, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Caenorhabditis elegans</term><term>Marqueurs biologiques</term><term>Mutation</term><term>Sélection génétique</term><term>Techniques de transfert de gènes</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Bordeaux</li></settlement></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Cornes, Eric" sort="Cornes, Eric" uniqKey="Cornes E" first="Eric" last="Cornes">Eric Cornes</name></region><name sortKey="Dupuy, Denis" sort="Dupuy, Denis" uniqKey="Dupuy D" first="Denis" last="Dupuy">Denis Dupuy</name><name sortKey="Quere, Cecile A L" sort="Quere, Cecile A L" uniqKey="Quere C" first="Cécile A L" last="Quéré">Cécile A L. Quéré</name></country><country name="Australie"><noRegion><name sortKey="Giordano Santini, Rosina" sort="Giordano Santini, Rosina" uniqKey="Giordano Santini R" first="Rosina" last="Giordano-Santini">Rosina Giordano-Santini</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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