Concerted Changes in the Phosphoproteome and Metabolome Under Different CO2/O2 Gaseous Conditions in Arabidopsis Rosettes.
Identifieur interne : 002181 ( Main/Exploration ); précédent : 002180; suivant : 002182Concerted Changes in the Phosphoproteome and Metabolome Under Different CO2/O2 Gaseous Conditions in Arabidopsis Rosettes.
Auteurs : Cyril Abadie [Australie] ; Samuel Mainguet [France] ; Marlène Davanture [France] ; Michael Hodges [France] ; Michel Zivy [France] ; Guillaume Tcherkez [Australie]Source :
- Plant & cell physiology [ 1471-9053 ] ; 2016.
Descripteurs français
- KwdFr :
- Analyse multivariée, Arabidopsis (), Arabidopsis (métabolisme), Azote (métabolisme), Carbone (métabolisme), Dioxyde de carbone (pharmacologie), Feuilles de plante (), Feuilles de plante (métabolisme), Métabolome (), Oxygène (pharmacologie), Phosphopeptides (métabolisme), Phosphoprotéines (métabolisme), Photosynthèse (), Protéome (métabolisme), Protéomique.
- MESH :
- métabolisme : Arabidopsis, Azote, Carbone, Feuilles de plante, Phosphopeptides, Phosphoprotéines, Protéome.
- pharmacologie : Dioxyde de carbone, Oxygène.
- Analyse multivariée, Arabidopsis, Feuilles de plante, Métabolome, Photosynthèse, Protéomique.
English descriptors
- KwdEn :
- Arabidopsis (drug effects), Arabidopsis (metabolism), Carbon (metabolism), Carbon Dioxide (pharmacology), Metabolome (drug effects), Multivariate Analysis, Nitrogen (metabolism), Oxygen (pharmacology), Phosphopeptides (metabolism), Phosphoproteins (metabolism), Photosynthesis (drug effects), Plant Leaves (drug effects), Plant Leaves (metabolism), Proteome (metabolism), Proteomics.
- MESH :
- chemical , metabolism : Carbon, Nitrogen, Phosphopeptides, Phosphoproteins, Proteome.
- drug effects : Arabidopsis, Metabolome, Photosynthesis, Plant Leaves.
- metabolism : Arabidopsis, Plant Leaves.
- chemical , pharmacology : Carbon Dioxide, Oxygen.
- Multivariate Analysis, Proteomics.
Abstract
Considerable efforts are currently devoted to understanding the regulation of primary carbon metabolism in plant leaves, which is known to change dramatically with environmental conditions, e.g. during light/dark transitions. Protein phosphorylation is believed to be a key factor in such a metabolic control. In fact, some studies have suggested modifications in the phosphorylation status of key enzymes in the dark compared with the light, or when photosynthesis varies. However, a general view of the phosphoproteome and reciprocal alterations in both the phosphoproteome and metabolome under a wide spectrum of CO2 and O2 conditions so as to vary both gross photosynthesis and photorespiration is currently lacking. Here, we used an instant sampling system and strictly controlled gaseous conditions to examine short-term metabolome and phosphoproteome changes in Arabidopsis rosettes. We show that light/dark, CO2 and O2 mole fraction have differential effects on enzyme phosphorylation. Phosphorylation events that appear to be the most important to regulate metabolite contents when photosynthesis varies are those associated with sugar and pyruvate metabolism: sucrose and starch synthesis are major phosphorylation-controlled steps but pyruvate utilization (by phosphoenolpyruvate carboxylase and pyruvate dehydrogenase) and pyruvate reformation (by pyruvate orthophosphate dikinase) are also subjected to phosphorylation control. Our results thus show that the phosphoproteome response to light/dark transition and gaseous conditions (CO2, O2) contributes to the rapid adjustment of major pathways of primary C metabolism.
DOI: 10.1093/pcp/pcw086
PubMed: 27903807
Affiliations:
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CO2/O2 Gaseous Conditions in Arabidopsis Rosettes.</title><author><name sortKey="Abadie, Cyril" sort="Abadie, Cyril" uniqKey="Abadie C" first="Cyril" last="Abadie">Cyril Abadie</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra ACT 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra ACT 2601</wicri:regionArea><wicri:noRegion>Canberra ACT 2601</wicri:noRegion></affiliation></author><author><name sortKey="Mainguet, Samuel" sort="Mainguet, Samuel" uniqKey="Mainguet S" first="Samuel" last="Mainguet">Samuel Mainguet</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Plant Sciences Paris-Saclay, UMR 9213/UMR1403, Université Paris Sud, CNRS-INRA, Université d'Evry, Université Paris-Diderot, Bâtiment 630, 91405 Orsay Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Plant Sciences Paris-Saclay, UMR 9213/UMR1403, Université Paris Sud, CNRS-INRA, Université d'Evry, Université Paris-Diderot, Bâtiment 630, 91405 Orsay Cedex</wicri:regionArea><wicri:noRegion>91405 Orsay Cedex</wicri:noRegion><orgName type="university">Université Paris-Sud</orgName><placeName><settlement type="city">Orsay</settlement><region type="region" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Davanture, Marlene" sort="Davanture, Marlene" uniqKey="Davanture M" first="Marlène" last="Davanture">Marlène Davanture</name><affiliation wicri:level="4"><nlm:affiliation>Plateforme PAPPSO, UMR 0320/8120 Génétique Quantitative et Evolution, Université Paris-Sud, CNRS-INRA-AgroParisTech, Le Moulon, 91190 Gif-sur-Yvette, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Plateforme PAPPSO, UMR 0320/8120 Génétique 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Cedex</wicri:regionArea><wicri:noRegion>91405 Orsay Cedex</wicri:noRegion><orgName type="university">Université Paris-Sud</orgName><placeName><settlement type="city">Orsay</settlement><region type="region" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Zivy, Michel" sort="Zivy, Michel" uniqKey="Zivy M" first="Michel" last="Zivy">Michel Zivy</name><affiliation wicri:level="4"><nlm:affiliation>Plateforme PAPPSO, UMR 0320/8120 Génétique Quantitative et Evolution, Université Paris-Sud, CNRS-INRA-AgroParisTech, Le Moulon, 91190 Gif-sur-Yvette, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Plateforme PAPPSO, UMR 0320/8120 Génétique Quantitative et Evolution, Université Paris-Sud, CNRS-INRA-AgroParisTech, Le Moulon, 91190 Gif-sur-Yvette</wicri:regionArea><wicri:noRegion>91190 Gif-sur-Yvette</wicri:noRegion><orgName type="university">Université Paris-Sud</orgName><placeName><settlement type="city">Orsay</settlement><region type="region" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Tcherkez, Guillaume" sort="Tcherkez, Guillaume" uniqKey="Tcherkez G" first="Guillaume" last="Tcherkez">Guillaume Tcherkez</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra ACT 2601, Australia guillaume.tcherkez@anu.edu.au.</nlm:affiliation><country wicri:rule="url">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra ACT 2601</wicri:regionArea><wicri:noRegion>Canberra ACT 2601</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="eISSN">1471-9053</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (drug effects)</term><term>Arabidopsis (metabolism)</term><term>Carbon (metabolism)</term><term>Carbon Dioxide (pharmacology)</term><term>Metabolome (drug effects)</term><term>Multivariate Analysis</term><term>Nitrogen (metabolism)</term><term>Oxygen (pharmacology)</term><term>Phosphopeptides (metabolism)</term><term>Phosphoproteins (metabolism)</term><term>Photosynthesis (drug effects)</term><term>Plant Leaves (drug effects)</term><term>Plant Leaves (metabolism)</term><term>Proteome (metabolism)</term><term>Proteomics</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse multivariée</term><term>Arabidopsis ()</term><term>Arabidopsis (métabolisme)</term><term>Azote (métabolisme)</term><term>Carbone (métabolisme)</term><term>Dioxyde de carbone (pharmacologie)</term><term>Feuilles de plante ()</term><term>Feuilles de plante (métabolisme)</term><term>Métabolome ()</term><term>Oxygène (pharmacologie)</term><term>Phosphopeptides (métabolisme)</term><term>Phosphoprotéines (métabolisme)</term><term>Photosynthèse ()</term><term>Protéome (métabolisme)</term><term>Protéomique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Nitrogen</term><term>Phosphopeptides</term><term>Phosphoproteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Arabidopsis</term><term>Metabolome</term><term>Photosynthesis</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Azote</term><term>Carbone</term><term>Feuilles de plante</term><term>Phosphopeptides</term><term>Phosphoprotéines</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Dioxyde de carbone</term><term>Oxygène</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Carbon Dioxide</term><term>Oxygen</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Multivariate Analysis</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse multivariée</term><term>Arabidopsis</term><term>Feuilles de plante</term><term>Métabolome</term><term>Photosynthèse</term><term>Protéomique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Considerable efforts are currently devoted to understanding the regulation of primary carbon metabolism in plant leaves, which is known to change dramatically with environmental conditions, e.g. during light/dark transitions. Protein phosphorylation is believed to be a key factor in such a metabolic control. In fact, some studies have suggested modifications in the phosphorylation status of key enzymes in the dark compared with the light, or when photosynthesis varies. However, a general view of the phosphoproteome and reciprocal alterations in both the phosphoproteome and metabolome under a wide spectrum of CO2 and O2 conditions so as to vary both gross photosynthesis and photorespiration is currently lacking. Here, we used an instant sampling system and strictly controlled gaseous conditions to examine short-term metabolome and phosphoproteome changes in Arabidopsis rosettes. We show that light/dark, CO2 and O2 mole fraction have differential effects on enzyme phosphorylation. Phosphorylation events that appear to be the most important to regulate metabolite contents when photosynthesis varies are those associated with sugar and pyruvate metabolism: sucrose and starch synthesis are major phosphorylation-controlled steps but pyruvate utilization (by phosphoenolpyruvate carboxylase and pyruvate dehydrogenase) and pyruvate reformation (by pyruvate orthophosphate dikinase) are also subjected to phosphorylation control. Our results thus show that the phosphoproteome response to light/dark transition and gaseous conditions (CO2, O2) contributes to the rapid adjustment of major pathways of primary C metabolism.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Orsay</li></settlement><orgName><li>Université Paris-Sud</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Abadie, Cyril" sort="Abadie, Cyril" uniqKey="Abadie C" first="Cyril" last="Abadie">Cyril Abadie</name></noRegion><name sortKey="Tcherkez, Guillaume" sort="Tcherkez, Guillaume" uniqKey="Tcherkez G" first="Guillaume" last="Tcherkez">Guillaume Tcherkez</name></country><country name="France"><region name="Île-de-France"><name sortKey="Mainguet, Samuel" sort="Mainguet, Samuel" uniqKey="Mainguet S" first="Samuel" last="Mainguet">Samuel Mainguet</name></region><name sortKey="Davanture, Marlene" sort="Davanture, Marlene" uniqKey="Davanture M" first="Marlène" last="Davanture">Marlène Davanture</name><name sortKey="Hodges, Michael" sort="Hodges, Michael" uniqKey="Hodges M" first="Michael" last="Hodges">Michael Hodges</name><name sortKey="Zivy, Michel" sort="Zivy, Michel" uniqKey="Zivy M" first="Michel" last="Zivy">Michel Zivy</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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