Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response
Identifieur interne : 001D24 ( Main/Exploration ); précédent : 001D23; suivant : 001D25Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response
Auteurs : Jana Jones [Australie] ; Mehdi Mirzaei [Australie] ; Prathiba Ravishankar [Australie] ; Dylan Xavier [Australie] ; Do Seon Lim [Corée du Sud] ; Dong Hoon Shin [Corée du Sud] ; Raffaella Bianucci [Italie, France] ; Paul A. Haynes [Australie]Source :
- Philosophical transactions. Series A, Mathematical, physical, and engineering sciences [ 1364-503X ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Inflammation, Peau.
- immunologie : Inflammation.
- métabolisme : Inflammation, Muscles, Peau.
- Biopsie, Femelle, Gene Ontology, Humains, Immunité innée, Maladie aigüe, Momies, Mâle, Protéomique, Égypte.
English descriptors
- KwdEn :
- MESH :
- immunology : Inflammation.
- metabolism : Inflammation, Muscles, Skin.
- pathology : Inflammation, Skin.
- Acute Disease, Biopsy, Egypt, Female, Gene Ontology, Humans, Immunity, Innate, Male, Mummies, Proteomics.
Abstract
We performed proteomics analysis on four skin and one muscle tissue samples taken from three ancient Egyptian mummies of the first intermediate period, approximately 4200 years old. The mummies were first dated by radiocarbon dating of the accompany-\break ing textiles, and morphologically examined by scanning electron microscopy of additional skin samples. Proteins were extracted, separated on SDS–PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) gels, and in-gel digested with trypsin. The resulting peptides were analysed using nanoflow high-performance liquid chromatography–mass spectrometry. We identified a total of 230 unique proteins from the five samples, which consisted of 132 unique protein identifications. We found a large number of collagens, which was confirmed by our microscopy data, and is in agreement with previous studies showing that collagens are very long-lived. As expected, we also found a large number of keratins. We identified numerous proteins that provide evidence of activation of the innate immunity system in two of the mummies, one of which also contained proteins indicating severe tissue inflammation, possibly indicative of an infection that we can speculate may have been related to the cause of death.
This article is part of the themed issue ‘Quantitative mass spectrometry’.
Url:
DOI: 10.1098/rsta.2015.0373
PubMed: 27644972
PubMed Central: 5031639
Affiliations:
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id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ravishankar, Prathiba" sort="Ravishankar, Prathiba" uniqKey="Ravishankar P" first="Prathiba" last="Ravishankar">Prathiba Ravishankar</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xavier, Dylan" sort="Xavier, Dylan" uniqKey="Xavier D" first="Dylan" last="Xavier">Dylan Xavier</name><affiliation wicri:level="1"><nlm:aff id="af3"><addr-line>Australian Proteome Analysis Facility</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do Seon" last="Lim">Do Seon Lim</name><affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Department of Dental Hygiene, College of Health Sciences</addr-line>,<institution>Eulji University</institution>,<addr-line>Sungnam</addr-line>,<country>South Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name><affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Department of Anatomy</addr-line>,<institution>Seoul National University, College of Medicine</institution>,<addr-line>Seoul</addr-line>,<country>South Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bianucci, Raffaella" sort="Bianucci, Raffaella" uniqKey="Bianucci R" first="Raffaella" last="Bianucci">Raffaella Bianucci</name><affiliation wicri:level="1"><nlm:aff id="af6"><addr-line>Department of Public Health and Paediatric Sciences, Legal Medicine Section</addr-line>,<institution>University of Turin</institution>,<addr-line>10126 Turin</addr-line>,<country>Italy</country></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af7"><institution>UMR 7268, Laboratoire 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Haynes</name><affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Department of Chemistry and Biomolecular Sciences</addr-line>,<institution>Macquarie University</institution>,<addr-line>North Ryde, NSW 2109</addr-line>,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Philosophical transactions. Series A, Mathematical, physical, and engineering sciences</title><idno type="ISSN">1364-503X</idno><idno type="eISSN">1471-2962</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute Disease</term><term>Biopsy</term><term>Egypt</term><term>Female</term><term>Gene Ontology</term><term>Humans</term><term>Immunity, Innate</term><term>Inflammation (immunology)</term><term>Inflammation (metabolism)</term><term>Inflammation (pathology)</term><term>Male</term><term>Mummies</term><term>Muscles (metabolism)</term><term>Proteomics</term><term>Skin (metabolism)</term><term>Skin (pathology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biopsie</term><term>Femelle</term><term>Gene Ontology</term><term>Humains</term><term>Immunité innée</term><term>Inflammation (anatomopathologie)</term><term>Inflammation (immunologie)</term><term>Inflammation (métabolisme)</term><term>Maladie aigüe</term><term>Momies</term><term>Muscles (métabolisme)</term><term>Mâle</term><term>Peau (anatomopathologie)</term><term>Peau (métabolisme)</term><term>Protéomique</term><term>Égypte</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Inflammation</term><term>Peau</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Inflammation</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Inflammation</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Inflammation</term><term>Muscles</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Inflammation</term><term>Muscles</term><term>Peau</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Inflammation</term><term>Skin</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acute Disease</term><term>Biopsy</term><term>Egypt</term><term>Female</term><term>Gene Ontology</term><term>Humans</term><term>Immunity, Innate</term><term>Male</term><term>Mummies</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biopsie</term><term>Femelle</term><term>Gene Ontology</term><term>Humains</term><term>Immunité innée</term><term>Maladie aigüe</term><term>Momies</term><term>Mâle</term><term>Protéomique</term><term>Égypte</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We performed proteomics analysis on four skin and one muscle tissue samples taken from three ancient Egyptian mummies of the first intermediate period, approximately 4200 years old. The mummies were first dated by radiocarbon dating of the accompany-\break ing textiles, and morphologically examined by scanning electron microscopy of additional skin samples. Proteins were extracted, separated on SDS–PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) gels, and in-gel digested with trypsin. The resulting peptides were analysed using nanoflow high-performance liquid chromatography–mass spectrometry. We identified a total of 230 unique proteins from the five samples, which consisted of 132 unique protein identifications. We found a large number of collagens, which was confirmed by our microscopy data, and is in agreement with previous studies showing that collagens are very long-lived. As expected, we also found a large number of keratins. We identified numerous proteins that provide evidence of activation of the innate immunity system in two of the mummies, one of which also contained proteins indicating severe tissue inflammation, possibly indicative of an infection that we can speculate may have been related to the cause of death.</p><p>This article is part of the themed issue ‘Quantitative mass spectrometry’.</p></div></front></TEI><affiliations><list><country><li>Australie</li><li>Corée du Sud</li><li>France</li><li>Italie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Jones, Jana" sort="Jones, Jana" uniqKey="Jones J" first="Jana" last="Jones">Jana Jones</name></noRegion><name sortKey="Haynes, Paul A" sort="Haynes, Paul A" uniqKey="Haynes P" first="Paul A." last="Haynes">Paul A. Haynes</name><name sortKey="Mirzaei, Mehdi" sort="Mirzaei, Mehdi" uniqKey="Mirzaei M" first="Mehdi" last="Mirzaei">Mehdi Mirzaei</name><name sortKey="Ravishankar, Prathiba" sort="Ravishankar, Prathiba" uniqKey="Ravishankar P" first="Prathiba" last="Ravishankar">Prathiba Ravishankar</name><name sortKey="Xavier, Dylan" sort="Xavier, Dylan" uniqKey="Xavier D" first="Dylan" last="Xavier">Dylan Xavier</name></country><country name="Corée du Sud"><noRegion><name sortKey="Lim, Do Seon" sort="Lim, Do Seon" uniqKey="Lim D" first="Do Seon" last="Lim">Do Seon Lim</name></noRegion><name sortKey="Shin, Dong Hoon" sort="Shin, Dong Hoon" uniqKey="Shin D" first="Dong Hoon" last="Shin">Dong Hoon Shin</name></country><country name="Italie"><noRegion><name sortKey="Bianucci, Raffaella" sort="Bianucci, Raffaella" uniqKey="Bianucci R" first="Raffaella" last="Bianucci">Raffaella Bianucci</name></noRegion></country><country name="France"><noRegion><name sortKey="Bianucci, Raffaella" sort="Bianucci, Raffaella" uniqKey="Bianucci R" first="Raffaella" last="Bianucci">Raffaella Bianucci</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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