Endothiovibrio diazotrophicus gen. nov., sp. nov., a novel nitrogen-fixing, sulfur-oxidizing gammaproteobacterium isolated from a salt marsh.
Identifieur interne : 000D29 ( Main/Exploration ); précédent : 000D28; suivant : 000D30Endothiovibrio diazotrophicus gen. nov., sp. nov., a novel nitrogen-fixing, sulfur-oxidizing gammaproteobacterium isolated from a salt marsh.
Auteurs : Dennis A. Bazylinski [États-Unis] ; Viviana Morillo [États-Unis] ; Christopher T. Lefèvre [France] ; Nathan Viloria [États-Unis] ; Bradley L. Dubbels [États-Unis] ; Timothy J. Williams [Australie]Source :
- International journal of systematic and evolutionary microbiology [ 1466-5034 ] ; 2017.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ARN ribosomique 16S (génétique), Acides gras (), Analyse de séquence d'ADN, Azote (métabolisme), Composition en bases nucléiques, Fixation de l'azote, Gammaproteobacteria (), Gammaproteobacteria (génétique), Gammaproteobacteria (isolement et purification), Massachusetts, Microbiologie de l'eau, Phospholipides (), Phylogénie, Soufre (métabolisme), Techniques de typage bactérien, Zones humides.
- MESH :
- génétique : ADN bactérien, ARN ribosomique 16S, Gammaproteobacteria.
- isolement et purification : Gammaproteobacteria.
- métabolisme : Azote, Soufre.
- Acides gras, Analyse de séquence d'ADN, Composition en bases nucléiques, Fixation de l'azote, Gammaproteobacteria, Massachusetts, Microbiologie de l'eau, Phospholipides, Phylogénie, Techniques de typage bactérien, Zones humides.
English descriptors
- KwdEn :
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial (genetics), Fatty Acids (chemistry), Gammaproteobacteria (classification), Gammaproteobacteria (genetics), Gammaproteobacteria (isolation & purification), Massachusetts, Nitrogen (metabolism), Nitrogen Fixation, Phospholipids (chemistry), Phylogeny, RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA, Sulfur (metabolism), Water Microbiology, Wetlands.
- MESH :
- chemical , chemistry : Fatty Acids, Phospholipids.
- chemical , genetics : DNA, Bacterial, RNA, Ribosomal, 16S.
- classification : Gammaproteobacteria.
- genetics : Gammaproteobacteria.
- isolation & purification : Gammaproteobacteria.
- chemical , metabolism : Nitrogen, Sulfur.
- Bacterial Typing Techniques, Base Composition, Massachusetts, Nitrogen Fixation, Phylogeny, Sequence Analysis, DNA, Water Microbiology, Wetlands.
Abstract
A novel non-phototrophic, marine, sulfur-oxidizing bacterium, strain S-1T, was isolated from a coastal salt marsh in Massachusetts, USA. Cells are Gram-stain-negative vibrios motile by means of a single polar unsheathed flagellum. S-1T is an obligate microaerophile with limited metabolic capacity. It grows chemolithoautotrophically utilizing sulfide and thiosulfate as electron donors, converting these compounds to sulfate, and the Calvin-Benson-Bassham cycle for carbon fixation. Cells of S-1T did not grow on any of a large number of organic carbon sources and there was no evidence for chemoorganoheterotrophic growth. Cells produced internal sulfur globules during growth on sulfide and thiosulfate. S-1T is strongly diazotrophic, as demonstrated by 15N2 fixation and acetylene reduction activity by cells when a fixed nitrogen source is absent from the growth medium. The marine nature of this organism is evident from its ability to grow in 10 to 100 % artificial seawater but not at lower concentrations and NaCl alone cannot substitute for sea salts. The major cellular fatty acids are C16 : 1ω7c, C16 : 0, and C18 : 1ω7c. Phosphatidylethanolamine and phosphatidylglycerol are the major polar lipids. Q8 is the only respiratory quinone. S-1T genomic DNA has a G+C content of 67.6 mol%. Based on its 16S rRNA gene sequence, S-1T shows the closest phylogenetic relationship to non-phototrophic species within the family Thioalkalispiraceae of the class Gammaproteobacteria. The name Endothiovibrio diazotrophicus is proposed for this organism, with S-1T as the type strain (ATCC BAA-1439T=JCM 17961T).
DOI: 10.1099/ijsem.0.001743
PubMed: 27983471
Affiliations:
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Le document en format XML
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Bazylinski</name><affiliation wicri:level="1"><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004</wicri:regionArea><wicri:noRegion>Nevada 89154-4004</wicri:noRegion></affiliation></author><author><name sortKey="Morillo, Viviana" sort="Morillo, Viviana" uniqKey="Morillo V" first="Viviana" last="Morillo">Viviana Morillo</name><affiliation wicri:level="1"><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004</wicri:regionArea><wicri:noRegion>Nevada 89154-4004</wicri:noRegion></affiliation></author><author><name sortKey="Lefevre, Christopher T" sort="Lefevre, Christopher T" uniqKey="Lefevre C" first="Christopher T" last="Lefèvre">Christopher T. 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Bazylinski</name><affiliation wicri:level="1"><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004</wicri:regionArea><wicri:noRegion>Nevada 89154-4004</wicri:noRegion></affiliation></author><author><name sortKey="Morillo, Viviana" sort="Morillo, Viviana" uniqKey="Morillo V" first="Viviana" last="Morillo">Viviana Morillo</name><affiliation wicri:level="1"><nlm:affiliation>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>1School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, Nevada 89154-4004</wicri:regionArea><wicri:noRegion>Nevada 89154-4004</wicri:noRegion></affiliation></author><author><name sortKey="Lefevre, Christopher T" sort="Lefevre, Christopher T" uniqKey="Lefevre C" first="Christopher T" last="Lefèvre">Christopher T. 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Dubbels</name><affiliation wicri:level="1"><nlm:affiliation>3Novozymes North America Inc., 9000 Development Drive, Morrisville, North Carolina 27560, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>3Novozymes North America Inc., 9000 Development Drive, Morrisville, North Carolina 27560</wicri:regionArea><wicri:noRegion>North Carolina 27560</wicri:noRegion></affiliation></author><author><name sortKey="Williams, Timothy J" sort="Williams, Timothy J" uniqKey="Williams T" first="Timothy J" last="Williams">Timothy J. Williams</name><affiliation wicri:level="1"><nlm:affiliation>4School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>4School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052</wicri:regionArea><wicri:noRegion>Sydney NSW 2052</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of systematic and evolutionary microbiology</title><idno type="eISSN">1466-5034</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Base Composition</term><term>DNA, Bacterial (genetics)</term><term>Fatty Acids (chemistry)</term><term>Gammaproteobacteria (classification)</term><term>Gammaproteobacteria (genetics)</term><term>Gammaproteobacteria (isolation & purification)</term><term>Massachusetts</term><term>Nitrogen (metabolism)</term><term>Nitrogen Fixation</term><term>Phospholipids (chemistry)</term><term>Phylogeny</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA</term><term>Sulfur (metabolism)</term><term>Water Microbiology</term><term>Wetlands</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>ARN ribosomique 16S (génétique)</term><term>Acides gras ()</term><term>Analyse de séquence d'ADN</term><term>Azote (métabolisme)</term><term>Composition en bases nucléiques</term><term>Fixation de l'azote</term><term>Gammaproteobacteria ()</term><term>Gammaproteobacteria (génétique)</term><term>Gammaproteobacteria (isolement et purification)</term><term>Massachusetts</term><term>Microbiologie de l'eau</term><term>Phospholipides ()</term><term>Phylogénie</term><term>Soufre (métabolisme)</term><term>Techniques de typage bactérien</term><term>Zones humides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fatty Acids</term><term>Phospholipids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>ARN ribosomique 16S</term><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Gammaproteobacteria</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term><term>Sulfur</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Soufre</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Base Composition</term><term>Massachusetts</term><term>Nitrogen Fixation</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Water Microbiology</term><term>Wetlands</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acides gras</term><term>Analyse de séquence d'ADN</term><term>Composition en bases nucléiques</term><term>Fixation de l'azote</term><term>Gammaproteobacteria</term><term>Massachusetts</term><term>Microbiologie de l'eau</term><term>Phospholipides</term><term>Phylogénie</term><term>Techniques de typage bactérien</term><term>Zones humides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel non-phototrophic, marine, sulfur-oxidizing bacterium, strain S-1T, was isolated from a coastal salt marsh in Massachusetts, USA. Cells are Gram-stain-negative vibrios motile by means of a single polar unsheathed flagellum. S-1T is an obligate microaerophile with limited metabolic capacity. It grows chemolithoautotrophically utilizing sulfide and thiosulfate as electron donors, converting these compounds to sulfate, and the Calvin-Benson-Bassham cycle for carbon fixation. Cells of S-1T did not grow on any of a large number of organic carbon sources and there was no evidence for chemoorganoheterotrophic growth. Cells produced internal sulfur globules during growth on sulfide and thiosulfate. S-1T is strongly diazotrophic, as demonstrated by 15N2 fixation and acetylene reduction activity by cells when a fixed nitrogen source is absent from the growth medium. The marine nature of this organism is evident from its ability to grow in 10 to 100 % artificial seawater but not at lower concentrations and NaCl alone cannot substitute for sea salts. The major cellular fatty acids are C16 : 1ω7c, C16 : 0, and C18 : 1ω7c. Phosphatidylethanolamine and phosphatidylglycerol are the major polar lipids. Q8 is the only respiratory quinone. S-1T genomic DNA has a G+C content of 67.6 mol%. Based on its 16S rRNA gene sequence, S-1T shows the closest phylogenetic relationship to non-phototrophic species within the family Thioalkalispiraceae of the class Gammaproteobacteria. The name Endothiovibrio diazotrophicus is proposed for this organism, with S-1T as the type strain (ATCC BAA-1439T=JCM 17961T).</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Bazylinski, Dennis A" sort="Bazylinski, Dennis A" uniqKey="Bazylinski D" first="Dennis A" last="Bazylinski">Dennis A. Bazylinski</name></noRegion><name sortKey="Dubbels, Bradley L" sort="Dubbels, Bradley L" uniqKey="Dubbels B" first="Bradley L" last="Dubbels">Bradley L. Dubbels</name><name sortKey="Morillo, Viviana" sort="Morillo, Viviana" uniqKey="Morillo V" first="Viviana" last="Morillo">Viviana Morillo</name><name sortKey="Viloria, Nathan" sort="Viloria, Nathan" uniqKey="Viloria N" first="Nathan" last="Viloria">Nathan Viloria</name></country><country name="France"><noRegion><name sortKey="Lefevre, Christopher T" sort="Lefevre, Christopher T" uniqKey="Lefevre C" first="Christopher T" last="Lefèvre">Christopher T. Lefèvre</name></noRegion></country><country name="Australie"><noRegion><name sortKey="Williams, Timothy J" sort="Williams, Timothy J" uniqKey="Williams T" first="Timothy J" last="Williams">Timothy J. Williams</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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