Ensa controls S-phase length by modulating Treslin levels
Identifieur interne : 000D25 ( Main/Exploration ); précédent : 000D24; suivant : 000D26Ensa controls S-phase length by modulating Treslin levels
Auteurs : Sophie Charrasse [France] ; Aicha Gharbi-Ayachi [France] ; Andrew Burgess ; Jorge Vera [France] ; Khaled Hached [France] ; Peggy Raynaud [France] ; Etienne Schwob [France] ; Thierry Lorca [France] ; Anna Castro [France]Source :
- Nature Communications [ 2041-1723 ] ; 2017.
Abstract
The Greatwall/Ensa/PP2A-B55 pathway is essential for controlling mitotic substrate phosphorylation and mitotic entry. Here, we investigate the effect of the knockdown of the Gwl substrate, Ensa, in human cells. Unexpectedly, Ensa knockdown promotes a dramatic extension of S phase associated with a lowered density of replication forks. Notably, Ensa depletion results in a decrease of Treslin levels, a pivotal protein for the firing of replication origins. Accordingly, the extended S phase in Ensa-depleted cells is completely rescued by the overexpression of Treslin. Our data herein reveal a new mechanism by which normal cells regulate S-phase duration by controlling the ubiquitin-proteasome degradation of Treslin in a Gwl/Ensa-dependent pathway.
Url:
DOI: 10.1038/s41467-017-00339-4
PubMed: 28785014
PubMed Central: 5547116
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p id="Par1">The Greatwall/Ensa/PP2A-B55 pathway is essential for controlling mitotic substrate phosphorylation and mitotic entry. Here, we investigate the effect of the knockdown of the Gwl substrate, Ensa, in human cells. Unexpectedly, Ensa knockdown promotes a dramatic extension of S phase associated with a lowered density of replication forks. Notably, Ensa depletion results in a decrease of Treslin levels, a pivotal protein for the firing of replication origins. Accordingly, the extended S phase in Ensa-depleted cells is completely rescued by the overexpression of Treslin. Our data herein reveal a new mechanism by which normal cells regulate S-phase duration by controlling the ubiquitin-proteasome degradation of Treslin in a Gwl/Ensa-dependent pathway.</p>
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</analytic>
</biblStruct>
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</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Languedoc-Roussillon</li>
<li>Occitanie (région administrative)</li>
</region>
<settlement><li>Montpellier</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Burgess, Andrew" sort="Burgess, Andrew" uniqKey="Burgess A" first="Andrew" last="Burgess">Andrew Burgess</name>
</noCountry>
<country name="France"><region name="Occitanie (région administrative)"><name sortKey="Charrasse, Sophie" sort="Charrasse, Sophie" uniqKey="Charrasse S" first="Sophie" last="Charrasse">Sophie Charrasse</name>
</region>
<name sortKey="Castro, Anna" sort="Castro, Anna" uniqKey="Castro A" first="Anna" last="Castro">Anna Castro</name>
<name sortKey="Gharbi Ayachi, Aicha" sort="Gharbi Ayachi, Aicha" uniqKey="Gharbi Ayachi A" first="Aicha" last="Gharbi-Ayachi">Aicha Gharbi-Ayachi</name>
<name sortKey="Hached, Khaled" sort="Hached, Khaled" uniqKey="Hached K" first="Khaled" last="Hached">Khaled Hached</name>
<name sortKey="Lorca, Thierry" sort="Lorca, Thierry" uniqKey="Lorca T" first="Thierry" last="Lorca">Thierry Lorca</name>
<name sortKey="Raynaud, Peggy" sort="Raynaud, Peggy" uniqKey="Raynaud P" first="Peggy" last="Raynaud">Peggy Raynaud</name>
<name sortKey="Schwob, Etienne" sort="Schwob, Etienne" uniqKey="Schwob E" first="Etienne" last="Schwob">Etienne Schwob</name>
<name sortKey="Vera, Jorge" sort="Vera, Jorge" uniqKey="Vera J" first="Jorge" last="Vera">Jorge Vera</name>
</country>
</tree>
</affiliations>
</record>
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