Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats
Identifieur interne : 000C90 ( Main/Exploration ); précédent : 000C89; suivant : 000C91Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats
Auteurs : Stéphane Zingue [Cameroun] ; Chantal Beatrice Magne Nde ; Thomas Michel [France] ; Derek Tantoh Ndinteh [Afrique du Sud] ; Jules Tchatchou [Cameroun] ; Moïse Adamou [Cameroun] ; Xavier Fernandez [France] ; Fernand-Nestor Tchuenguem Fohouo [Cameroun] ; Colin Clyne ; Dieudonné Njamen [Cameroun, Afrique du Sud]Source :
- BMC Complementary and Alternative Medicine [ 1472-6882 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- pharmacologie : Oestrogènes, Propolis.
- traitement médicamenteux : Bouffées de chaleur.
- Abeilles, Animaux, Cameroun, Cellules HEK293, Femelle, Glandes mammaires animales, Humains, Ovariectomie, Propolis, Rat Wistar, Rats, Taille d'organe, Utérus, Vagin, Éthanol.
English descriptors
- KwdEn :
- Animals, Bees, Cameroon, Estrogens (pharmacology), Ethanol, Female, HEK293 Cells, Hot Flashes (drug therapy), Humans, Mammary Glands, Animal (drug effects), Organ Size (drug effects), Ovariectomy, Propolis (chemistry), Propolis (pharmacology), Rats, Rats, Wistar, Uterus (drug effects), Vagina (drug effects).
- MESH :
- chemical , chemistry : Propolis.
- chemical , pharmacology : Estrogens, Propolis.
- drug effects : Mammary Glands, Animal, Organ Size, Uterus, Vagina.
- drug therapy : Hot Flashes.
- Animals, Bees, Cameroon, Ethanol, Female, HEK293 Cells, Humans, Ovariectomy, Rats, Rats, Wistar.
Abstract
Since the biological properties of propolis depend to the plants that can be found in a specific region, propolis from unexplored regions attracts the attention of scientists. Ethanolic extract of Cameroonian propolis (EEP) is used to treat various ailments including gynecological problems and amenorrhea. Since there were no scientific data to support the above claims, the present study was therefore undertaken to assess estrogenic properties of Cameroonian propolis.
To achieve our goal, the ability of EEP to induce MCF-7 cells proliferation in E-screen assay as well as to activate estrogen receptors α (ERα) and β (ERβ) in cell-based reporter gene assays using human embryonic kidney cells (HEK293T) transfected with ERs was tested. Further, a 3-day uterotrophic assay was performed and the ability of EEP to alleviate hot flushes in ovariectomized adult rats was evaluated.
In vitro, EEP showed an antiestrogenic activity in both HEK293T ER-α and ER-β cells. In vivo, EEP induced a significant increase in a bell shape dose response manner of the uterine wet weight, the total protein levels in the uterus, the uterine and vaginal epithelium height and acini border cells of mammary gland with the presence of abundant eosinophil secretions. Moreover, EEP induced a significant decrease in the total number, average duration as well as frequency of hot flushes after 3 days of treatment in rat (equivalent to a month in woman). The dose of 150 mg/kg exhibited the most potent estrogenic effects among all the tested doses. The UPLC-HRMS analysis showed the presence of caffeic acid derivatives and trirtepernoids in EEP, which are well known endowed with estrogenic properties.
These results suggest that Ethanolic extract of Cameroonian propolis has estrogen-like effects in vivo and may alleviate some menopausal problems such as vaginal dryness and hot flushes.
Ethanol-extracted Cameroobian propolis exhibited in vitro and in vivo estrogen-like effects. This extract may contain promising phytoestrogens.
Url:
DOI: 10.1186/s12906-017-1568-8
PubMed: 28109283
PubMed Central: 5477728
Affiliations:
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Box 55, Maroua, Cameroon</nlm:aff><country xml:lang="fr">Cameroun</country><wicri:regionArea>P.O. Box 55, Maroua</wicri:regionArea><wicri:noRegion>Maroua</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2173 8504</institution-id><institution-id institution-id-type="GRID">grid.412661.6</institution-id><institution>Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science,</institution><institution>University of Yaounde 1,</institution></institution-wrap>P.O. Box 812, Yaoundé, Cameroon</nlm:aff><country xml:lang="fr">Cameroun</country><wicri:regionArea>P.O. Box 812, Yaoundé</wicri:regionArea><wicri:noRegion>Yaoundé</wicri:noRegion></affiliation></author><author><name sortKey="Nde, Chantal Beatrice Magne" sort="Nde, Chantal Beatrice Magne" uniqKey="Nde C" first="Chantal Beatrice Magne" last="Nde">Chantal Beatrice Magne Nde</name><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.452824.d</institution-id><institution></institution><institution>Hudson Institute of Medical Research,</institution></institution-wrap>27-31 Wright Street, Clayton, VIC 3168 Australia</nlm:aff><wicri:noCountry code="subfield">VIC 3168 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Michel, Thomas" sort="Michel, Thomas" uniqKey="Michel T" first="Thomas" last="Michel">Thomas Michel</name><affiliation wicri:level="3"><nlm:aff id="Aff4">Institute of Chemistry of Nice, Faculty of Science, University Côte d’Azur, UMR CNRS 7272, Valrose Park, Nice, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry of Nice, Faculty of Science, University Côte d’Azur, UMR CNRS 7272, Valrose Park, Nice</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Nice</settlement></placeName></affiliation></author><author><name sortKey="Ndinteh, Derek Tantoh" sort="Ndinteh, Derek Tantoh" uniqKey="Ndinteh D" first="Derek Tantoh" last="Ndinteh">Derek Tantoh Ndinteh</name><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0109 131X</institution-id><institution-id institution-id-type="GRID">grid.412988.e</institution-id><institution>Department of Applied Chemistry, Faculty of Sciences,</institution><institution>University of Johannesburg,</institution></institution-wrap>P.O. Box 17011, Doornfontein, 2028 South Africa</nlm:aff><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>P.O. Box 17011, Doornfontein</wicri:regionArea><wicri:noRegion>Doornfontein</wicri:noRegion></affiliation></author><author><name sortKey="Tchatchou, Jules" sort="Tchatchou, Jules" uniqKey="Tchatchou J" first="Jules" last="Tchatchou">Jules Tchatchou</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2173 8504</institution-id><institution-id institution-id-type="GRID">grid.412661.6</institution-id><institution>Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science,</institution><institution>University of Yaounde 1,</institution></institution-wrap>P.O. Box 812, Yaoundé, Cameroon</nlm:aff><country xml:lang="fr">Cameroun</country><wicri:regionArea>P.O. Box 812, Yaoundé</wicri:regionArea><wicri:noRegion>Yaoundé</wicri:noRegion></affiliation></author><author><name sortKey="Adamou, Moise" sort="Adamou, Moise" uniqKey="Adamou M" first="Moïse" last="Adamou">Moïse Adamou</name><affiliation wicri:level="1"><nlm:aff id="Aff6"><institution-wrap><institution-id institution-id-type="GRID">grid.440604.2</institution-id><institution>Department of Biological Sciences, Faculty of Science,</institution><institution>University of Ngaoundere,</institution></institution-wrap>P.O. Box 454, Ngaoundere, Cameroon</nlm:aff><country xml:lang="fr">Cameroun</country><wicri:regionArea>P.O. Box 454, Ngaoundere</wicri:regionArea><wicri:noRegion>Ngaoundere</wicri:noRegion></affiliation></author><author><name sortKey="Fernandez, Xavier" sort="Fernandez, Xavier" uniqKey="Fernandez X" first="Xavier" last="Fernandez">Xavier Fernandez</name><affiliation wicri:level="3"><nlm:aff id="Aff4">Institute of Chemistry of Nice, Faculty of Science, University Côte d’Azur, UMR CNRS 7272, Valrose Park, Nice, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry of Nice, Faculty of Science, University Côte d’Azur, UMR CNRS 7272, Valrose Park, Nice</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Nice</settlement></placeName></affiliation></author><author><name sortKey="Fohouo, Fernand Nestor Tchuenguem" sort="Fohouo, Fernand Nestor Tchuenguem" uniqKey="Fohouo F" first="Fernand-Nestor Tchuenguem" last="Fohouo">Fernand-Nestor Tchuenguem Fohouo</name><affiliation wicri:level="1"><nlm:aff id="Aff6"><institution-wrap><institution-id institution-id-type="GRID">grid.440604.2</institution-id><institution>Department of Biological Sciences, Faculty of Science,</institution><institution>University of Ngaoundere,</institution></institution-wrap>P.O. Box 454, Ngaoundere, Cameroon</nlm:aff><country xml:lang="fr">Cameroun</country><wicri:regionArea>P.O. Box 454, Ngaoundere</wicri:regionArea><wicri:noRegion>Ngaoundere</wicri:noRegion></affiliation></author><author><name sortKey="Clyne, Colin" sort="Clyne, Colin" uniqKey="Clyne C" first="Colin" last="Clyne">Colin Clyne</name><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.452824.d</institution-id><institution></institution><institution>Hudson Institute of Medical Research,</institution></institution-wrap>27-31 Wright Street, Clayton, VIC 3168 Australia</nlm:aff><wicri:noCountry code="subfield">VIC 3168 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Njamen, Dieudonne" sort="Njamen, Dieudonne" uniqKey="Njamen D" first="Dieudonné" last="Njamen">Dieudonné Njamen</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2173 8504</institution-id><institution-id institution-id-type="GRID">grid.412661.6</institution-id><institution>Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science,</institution><institution>University of Yaounde 1,</institution></institution-wrap>P.O. Box 812, Yaoundé, Cameroon</nlm:aff><country xml:lang="fr">Cameroun</country><wicri:regionArea>P.O. Box 812, Yaoundé</wicri:regionArea><wicri:noRegion>Yaoundé</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0109 131X</institution-id><institution-id institution-id-type="GRID">grid.412988.e</institution-id><institution>Department of Applied Chemistry, Faculty of Sciences,</institution><institution>University of Johannesburg,</institution></institution-wrap>P.O. Box 17011, Doornfontein, 2028 South Africa</nlm:aff><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>P.O. Box 17011, Doornfontein</wicri:regionArea><wicri:noRegion>Doornfontein</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC Complementary and Alternative Medicine</title><idno type="eISSN">1472-6882</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bees</term><term>Cameroon</term><term>Estrogens (pharmacology)</term><term>Ethanol</term><term>Female</term><term>HEK293 Cells</term><term>Hot Flashes (drug therapy)</term><term>Humans</term><term>Mammary Glands, Animal (drug effects)</term><term>Organ Size (drug effects)</term><term>Ovariectomy</term><term>Propolis (chemistry)</term><term>Propolis (pharmacology)</term><term>Rats</term><term>Rats, Wistar</term><term>Uterus (drug effects)</term><term>Vagina (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Abeilles</term><term>Animaux</term><term>Bouffées de chaleur (traitement médicamenteux)</term><term>Cameroun</term><term>Cellules HEK293</term><term>Femelle</term><term>Glandes mammaires animales ()</term><term>Humains</term><term>Oestrogènes (pharmacologie)</term><term>Ovariectomie</term><term>Propolis ()</term><term>Propolis (pharmacologie)</term><term>Rat Wistar</term><term>Rats</term><term>Taille d'organe ()</term><term>Utérus ()</term><term>Vagin ()</term><term>Éthanol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Propolis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Estrogens</term><term>Propolis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Mammary Glands, Animal</term><term>Organ Size</term><term>Uterus</term><term>Vagina</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Hot Flashes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oestrogènes</term><term>Propolis</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Bouffées de chaleur</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bees</term><term>Cameroon</term><term>Ethanol</term><term>Female</term><term>HEK293 Cells</term><term>Humans</term><term>Ovariectomy</term><term>Rats</term><term>Rats, Wistar</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Abeilles</term><term>Animaux</term><term>Cameroun</term><term>Cellules HEK293</term><term>Femelle</term><term>Glandes mammaires animales</term><term>Humains</term><term>Ovariectomie</term><term>Propolis</term><term>Rat Wistar</term><term>Rats</term><term>Taille d'organe</term><term>Utérus</term><term>Vagin</term><term>Éthanol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">Since the biological properties of propolis depend to the plants that can be found in a specific region, propolis from unexplored regions attracts the attention of scientists. Ethanolic extract of Cameroonian propolis (EEP) is used to treat various ailments including gynecological problems and amenorrhea. Since there were no scientific data to support the above claims, the present study was therefore undertaken to assess estrogenic properties of Cameroonian propolis.</p></sec><sec><title>Methods</title><p id="Par2">To achieve our goal, the ability of EEP to induce MCF-7 cells proliferation in E-screen assay as well as to activate estrogen receptors α (ERα) and β (ERβ) in cell-based reporter gene assays using human embryonic kidney cells (HEK293T) transfected with ERs was tested. Further, a 3-day uterotrophic assay was performed and the ability of EEP to alleviate hot flushes in ovariectomized adult rats was evaluated.</p></sec><sec><title>Results</title><p id="Par3">In vitro, EEP showed an antiestrogenic activity in both HEK293T ER-α and ER-β cells. In vivo, EEP induced a significant increase in a bell shape dose response manner of the uterine wet weight, the total protein levels in the uterus, the uterine and vaginal epithelium height and acini border cells of mammary gland with the presence of abundant eosinophil secretions. Moreover, EEP induced a significant decrease in the total number, average duration as well as frequency of hot flushes after 3 days of treatment in rat (equivalent to a month in woman). The dose of 150 mg/kg exhibited the most potent estrogenic effects among all the tested doses. The UPLC-HRMS analysis showed the presence of caffeic acid derivatives and trirtepernoids in EEP, which are well known endowed with estrogenic properties.</p></sec><sec><title>Conclusion</title><p id="Par4">These results suggest that Ethanolic extract of Cameroonian propolis has estrogen-like effects in vivo and may alleviate some menopausal problems such as vaginal dryness and hot flushes.</p></sec><sec><title>Graphical abstract</title><p id="Par5">Ethanol-extracted Cameroobian propolis exhibited in vitro and in vivo estrogen-like effects. 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sortKey="Gustafsson, J" uniqKey="Gustafsson J">JÅ Gustafsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okamoto, Y" uniqKey="Okamoto Y">Y Okamoto</name></author><author><name sortKey="Tobe, T" uniqKey="Tobe T">T Tobe</name></author><author><name sortKey="Ueda, K" uniqKey="Ueda K">K Ueda</name></author><author><name sortKey="Takada, T" uniqKey="Takada T">T Takada</name></author><author><name sortKey="Kojima, N" uniqKey="Kojima N">N Kojima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diel, P" uniqKey="Diel P">P Diel</name></author><author><name sortKey="Geis, Rb" uniqKey="Geis R">RB Geis</name></author><author><name sortKey="Caldarelli, A" uniqKey="Caldarelli A">A Caldarelli</name></author><author><name sortKey="Schmidt, S" uniqKey="Schmidt S">S Schmidt</name></author><author><name sortKey="Leschowsky, Ul" uniqKey="Leschowsky U">UL Leschowsky</name></author><author><name sortKey="Voss, A" uniqKey="Voss A">A 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Pakdel</name></author><author><name sortKey="Pedersen, Kl" uniqKey="Pedersen K">KL Pedersen</name></author><author><name sortKey="Perez, P" uniqKey="Perez P">P Perez</name></author><author><name sortKey="Skakke Boek, Ne" uniqKey="Skakke Boek N">NE Skakke-boek</name></author><author><name sortKey="Sonnenschein, C" uniqKey="Sonnenschein C">C Sonnenschein</name></author><author><name sortKey="Soto, Am" uniqKey="Soto A">AM Soto</name></author><author><name sortKey="Sumpter, Jp" uniqKey="Sumpter J">JP Sumpter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ito, K" uniqKey="Ito K">K Ito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heinemann, C" uniqKey="Heinemann C">C Heinemann</name></author><author><name sortKey="Reid, G" uniqKey="Reid G">G Reid</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Farid, A" uniqKey="Farid A">A Farid</name></author><author><name sortKey="Bambi, E" uniqKey="Bambi E">E Bambi</name></author><author><name sortKey="Dali, E" uniqKey="Dali E">E Dali</name></author><author><name sortKey="Essia, S" uniqKey="Essia S">S Essia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Almutairi, S" uniqKey="Almutairi S">S Almutairi</name></author><author><name sortKey="Eapen, B" uniqKey="Eapen B">B Eapen</name></author><author><name sortKey="Chundi, Ms" uniqKey="Chundi M">MS Chundi</name></author><author><name sortKey="Akhalil, A" uniqKey="Akhalil A">A Akhalil</name></author><author><name sortKey="Siheri, W" uniqKey="Siheri W">W Siheri</name></author><author><name sortKey="Clements, C" uniqKey="Clements C">C Clements</name></author><author><name sortKey="Fearnley, J" uniqKey="Fearnley J">J Fearnley</name></author><author><name sortKey="Watsona, Dg" uniqKey="Watsona D">DG Watsona</name></author><author><name sortKey="Edrada Ebel, R" uniqKey="Edrada Ebel R">R Edrada-Ebel</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Afrique du Sud</li><li>Cameroun</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Nice</li></settlement></list><tree><noCountry><name sortKey="Clyne, Colin" sort="Clyne, Colin" uniqKey="Clyne C" first="Colin" last="Clyne">Colin Clyne</name><name sortKey="Nde, Chantal Beatrice Magne" sort="Nde, Chantal Beatrice Magne" uniqKey="Nde C" first="Chantal Beatrice Magne" last="Nde">Chantal Beatrice Magne Nde</name></noCountry><country name="Cameroun"><noRegion><name sortKey="Zingue, Stephane" sort="Zingue, Stephane" uniqKey="Zingue S" first="Stéphane" last="Zingue">Stéphane Zingue</name></noRegion><name sortKey="Adamou, Moise" sort="Adamou, Moise" uniqKey="Adamou M" first="Moïse" last="Adamou">Moïse Adamou</name><name sortKey="Fohouo, Fernand Nestor Tchuenguem" sort="Fohouo, Fernand Nestor Tchuenguem" uniqKey="Fohouo F" first="Fernand-Nestor Tchuenguem" last="Fohouo">Fernand-Nestor Tchuenguem Fohouo</name><name sortKey="Njamen, Dieudonne" sort="Njamen, Dieudonne" uniqKey="Njamen D" first="Dieudonné" last="Njamen">Dieudonné Njamen</name><name sortKey="Tchatchou, Jules" sort="Tchatchou, Jules" uniqKey="Tchatchou J" first="Jules" last="Tchatchou">Jules Tchatchou</name><name sortKey="Zingue, Stephane" sort="Zingue, Stephane" uniqKey="Zingue S" first="Stéphane" last="Zingue">Stéphane Zingue</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Michel, Thomas" sort="Michel, Thomas" uniqKey="Michel T" first="Thomas" last="Michel">Thomas Michel</name></region><name sortKey="Fernandez, Xavier" sort="Fernandez, Xavier" uniqKey="Fernandez X" first="Xavier" last="Fernandez">Xavier Fernandez</name></country><country name="Afrique du Sud"><noRegion><name sortKey="Ndinteh, Derek Tantoh" sort="Ndinteh, Derek Tantoh" uniqKey="Ndinteh D" first="Derek Tantoh" last="Ndinteh">Derek Tantoh Ndinteh</name></noRegion><name sortKey="Njamen, Dieudonne" sort="Njamen, Dieudonne" uniqKey="Njamen D" first="Dieudonné" last="Njamen">Dieudonné Njamen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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