Watson-Crick Base Pair Radical Cation as a Model for Oxidative Damage in DNA.
Identifieur interne : 000050 ( Main/Exploration ); précédent : 000049; suivant : 000051Watson-Crick Base Pair Radical Cation as a Model for Oxidative Damage in DNA.
Auteurs : Linda Feketeová [Australie] ; Bun Chan ; George N. Khairallah [Australie] ; Vincent Steinmetz [France] ; Philippe Maitre [France] ; Leo Radom ; Richard A J. O'Hair [Australie]Source :
- The journal of physical chemistry letters [ 1948-7185 ] ; 2017.
Abstract
The deleterious cellular effects of ionizing radiation are well-known, but the mechanisms causing DNA damage are poorly understood. The accepted molecular events involve initial oxidation and deprotonation at guanine sites, triggering hydrogen atom abstraction reactions from the sugar moieties, causing DNA strand breaks. Probing the chemistry of the initially formed radical cation has been challenging. Here, we generate, spectroscopically characterize, and examine the reactivity of the Watson-Crick nucleobase pair radical cation in the gas phase. We observe rich chemistry, including proton transfer between the bases and propagation of the radical site in deoxyguanosine from the base to the sugar, thus rupturing the sugar. This first example of a gas-phase model system providing molecular-level details on the chemistry of an ionized DNA base pair paves the way toward a more complete understanding of molecular processes induced by radiation. It also highlights the role of radical propagation in chemistry, biology, and nanotechnology.
DOI: 10.1021/acs.jpclett.7b01251
PubMed: 28628332
Affiliations:
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O'Hair</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry, Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne , Parkville, Victoria 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne , Parkville, Victoria 3010</wicri:regionArea><wicri:noRegion>Victoria 3010</wicri:noRegion></affiliation></author></analytic><series><title level="j">The journal of physical chemistry letters</title><idno type="eISSN">1948-7185</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The deleterious cellular effects of ionizing radiation are well-known, but the mechanisms causing DNA damage are poorly understood. The accepted molecular events involve initial oxidation and deprotonation at guanine sites, triggering hydrogen atom abstraction reactions from the sugar moieties, causing DNA strand breaks. Probing the chemistry of the initially formed radical cation has been challenging. Here, we generate, spectroscopically characterize, and examine the reactivity of the Watson-Crick nucleobase pair radical cation in the gas phase. We observe rich chemistry, including proton transfer between the bases and propagation of the radical site in deoxyguanosine from the base to the sugar, thus rupturing the sugar. This first example of a gas-phase model system providing molecular-level details on the chemistry of an ionized DNA base pair paves the way toward a more complete understanding of molecular processes induced by radiation. It also highlights the role of radical propagation in chemistry, biology, and nanotechnology.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country></list><tree><noCountry><name sortKey="Chan, Bun" sort="Chan, Bun" uniqKey="Chan B" first="Bun" last="Chan">Bun Chan</name><name sortKey="Radom, Leo" sort="Radom, Leo" uniqKey="Radom L" first="Leo" last="Radom">Leo Radom</name></noCountry><country name="Australie"><noRegion><name sortKey="Feketeova, Linda" sort="Feketeova, Linda" uniqKey="Feketeova L" first="Linda" last="Feketeová">Linda Feketeová</name></noRegion><name sortKey="Khairallah, George N" sort="Khairallah, George N" uniqKey="Khairallah G" first="George N" last="Khairallah">George N. Khairallah</name><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A J" last="O'Hair">Richard A J. O'Hair</name></country><country name="France"><noRegion><name sortKey="Steinmetz, Vincent" sort="Steinmetz, Vincent" uniqKey="Steinmetz V" first="Vincent" last="Steinmetz">Vincent Steinmetz</name></noRegion><name sortKey="Maitre, Philippe" sort="Maitre, Philippe" uniqKey="Maitre P" first="Philippe" last="Maitre">Philippe Maitre</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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