Thymic Medulla Epithelial Cells Acquire Specific Markers by Post-Mitotic Maturation
Identifieur interne : 00D117 ( Main/Curation ); précédent : 00D116; suivant : 00D118Thymic Medulla Epithelial Cells Acquire Specific Markers by Post-Mitotic Maturation
Auteurs : Claude Penit [France] ; Bruno Lucas [France] ; Florence Vasseur [France] ; Theresa Rieker [Autriche] ; Richard L. Boyd [Australie]Source :
- Developmental Immunology [ 1044-6672 ] ; 1996.
Abstract
The development of thymocyte subsets and of the thymic epithelium in SCID and RAG-2-/– mice was monitored after normal bone-marrow-cell transfer. The kinetics of thymic reconstitution and their relationships with cell proliferation were investigated by using bromodeoxyuridine to detect DNA-synthesizing cells among lymphoid cells by 3-color flow cytometry, and in epithelial compartments by staining frozen sections. Thymocytes started to express CD8 and CD4 10 days after transfer, simultaneously with extensive proliferation. The first mature CD4+ single-positive cells were generated, from resting CD4+CD8+cells after day 15. During this day 10–15 period, many epithelial cells positive for cortexspecific or panepithelial markers were labeled with BrdUrd after pulse-injection. Organized medullary epithelium also developed after day,15, that is, synchronously with the appearance of mature thymocytes, but medullary cells were never found BrdUrd+. These results suggest that, in these models, the reconstitution of the thymic epithelial network proceeds through expansion of preexisting cortical or undifferentiated cells and by later maturation (acquisition of specific markers) of medullary cells. This last process is dependent of the presence of mature thymocytes.
Url:
DOI: 10.1155/1996/61035
PubMed: 8828009
PubMed Central: 2275969
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Boyd</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Pathology and Immunology</addr-line><addr-line>Monash Medical School</addr-line><addr-line>Commercial Road</addr-line><addr-line>Prahran</addr-line><addr-line>Melbourne</addr-line><addr-line>Victoria</addr-line><country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Developmental Immunology</title><idno type="ISSN">1044-6672</idno><imprint><date when="1996">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The development of thymocyte subsets and of the thymic epithelium in SCID and RAG-2<sup>-</sup>/– mice was monitored after normal bone-marrow-cell transfer. The kinetics of thymic
reconstitution and their relationships with cell proliferation were investigated by using
bromodeoxyuridine to detect DNA-synthesizing cells among lymphoid cells by 3-color
flow cytometry, and in epithelial compartments by staining frozen sections. Thymocytes
started to express CD8 and CD4 10 days after transfer, simultaneously with extensive proliferation.
The first mature CD4<sup>+</sup> single-positive cells were generated, from resting CD4<sup>+</sup>CD8<sup>+</sup>cells after day 15. During this day 10–15 period, many epithelial cells positive for cortexspecific
or panepithelial markers were labeled with BrdUrd after pulse-injection. Organized
medullary epithelium also developed after day,15, that is, synchronously with the
appearance of mature thymocytes, but medullary cells were never found BrdUrd<sup>+</sup>. These
results suggest that, in these models, the reconstitution of the thymic epithelial network
proceeds through expansion of preexisting cortical or undifferentiated cells and by later
maturation (acquisition of specific markers) of medullary cells. This last process is dependent
of the presence of mature thymocytes.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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