Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions.
Identifieur interne : 002240 ( Main/Curation ); précédent : 002239; suivant : 002241Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions.
Auteurs : Ken-Ichiro Kamei [Japon] ; Yoshie Koyama [Japon] ; Yumie Tokunaga [Japon] ; Yasumasa Mashimo [Japon] ; Momoko Yoshioka [Japon] ; Christopher Fockenberg [Japon] ; Rowland Mosbergen [Australie] ; Othmar Korn [Australie] ; Christine Wells [Australie] ; Yong Chen [Japon]Source :
- Advanced healthcare materials [ 2192-2659 ] ; 2016.
Abstract
Human pluripotent stem cells hold great promise for applications in drug discovery and regenerative medicine. Microfluidic technology is a promising approach for creating artificial microenvironments; however, although a proper 3D microenvironment is required to achieve robust control of cellular phenotypes, most current microfluidic devices provide only 2D cell culture and do not allow tuning of physical and chemical environmental cues simultaneously. Here, the authors report a 3D cellular microenvironment plate (3D-CEP), which consists of a microfluidic device filled with thermoresponsive poly(N-isopropylacrylamide)-β-poly(ethylene glycol) hydrogel (HG), which enables systematic tuning of both chemical and physical environmental cues as well as in situ cell monitoring. The authors show that H9 human embryonic stem cells (hESCs) and 253G1 human induced pluripotent stem cells in the HG/3D-CEP system maintain their pluripotent marker expression under HG/3D-CEP self-renewing conditions. Additionally, global gene expression analyses are used to elucidate small variations among different test environments. Interestingly, the authors find that treatment of H9 hESCs under HG/3D-CEP self-renewing conditions results in initiation of entry into the neural differentiation process by induction of PAX3 and OTX1 expression. The authors believe that this HG/3D-CEP system will serve as a versatile platform for developing targeted functional cell lines and facilitate advances in drug screening and regenerative medicine.
DOI: 10.1002/adhm.201600893
PubMed: 27775225
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001591
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001569
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001569
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :003D61
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :003D61
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003D61
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002239
Links to Exploration step
pubmed:27775225Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions.</title><author><name sortKey="Kamei, Ken Ichiro" sort="Kamei, Ken Ichiro" uniqKey="Kamei K" first="Ken-Ichiro" last="Kamei">Ken-Ichiro Kamei</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Koyama, Yoshie" sort="Koyama, Yoshie" uniqKey="Koyama Y" first="Yoshie" last="Koyama">Yoshie Koyama</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Tokunaga, Yumie" sort="Tokunaga, Yumie" uniqKey="Tokunaga Y" first="Yumie" last="Tokunaga">Yumie Tokunaga</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mashimo, Yasumasa" sort="Mashimo, Yasumasa" uniqKey="Mashimo Y" first="Yasumasa" last="Mashimo">Yasumasa Mashimo</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Yoshioka, Momoko" sort="Yoshioka, Momoko" uniqKey="Yoshioka M" first="Momoko" last="Yoshioka">Momoko Yoshioka</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Fockenberg, Christopher" sort="Fockenberg, Christopher" uniqKey="Fockenberg C" first="Christopher" last="Fockenberg">Christopher Fockenberg</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mosbergen, Rowland" sort="Mosbergen, Rowland" uniqKey="Mosbergen R" first="Rowland" last="Mosbergen">Rowland Mosbergen</name><affiliation wicri:level="1"><nlm:affiliation>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072</wicri:regionArea><wicri:noRegion>4072</wicri:noRegion></affiliation></author><author><name sortKey="Korn, Othmar" sort="Korn, Othmar" uniqKey="Korn O" first="Othmar" last="Korn">Othmar Korn</name><affiliation wicri:level="1"><nlm:affiliation>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072</wicri:regionArea><wicri:noRegion>4072</wicri:noRegion></affiliation></author><author><name sortKey="Wells, Christine" sort="Wells, Christine" uniqKey="Wells C" first="Christine" last="Wells">Christine Wells</name><affiliation wicri:level="1"><nlm:affiliation>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072</wicri:regionArea><wicri:noRegion>4072</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yong" sort="Chen, Yong" uniqKey="Chen Y" first="Yong" last="Chen">Yong Chen</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27775225</idno><idno type="pmid">27775225</idno><idno type="doi">10.1002/adhm.201600893</idno><idno type="wicri:Area/PubMed/Corpus">001591</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001591</idno><idno type="wicri:Area/PubMed/Curation">001569</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001569</idno><idno type="wicri:Area/PubMed/Checkpoint">001569</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001569</idno><idno type="wicri:Area/Ncbi/Merge">003D61</idno><idno type="wicri:Area/Ncbi/Curation">003D61</idno><idno type="wicri:Area/Ncbi/Checkpoint">003D61</idno><idno type="wicri:Area/Main/Merge">002239</idno><idno type="wicri:Area/Main/Curation">002240</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions.</title><author><name sortKey="Kamei, Ken Ichiro" sort="Kamei, Ken Ichiro" uniqKey="Kamei K" first="Ken-Ichiro" last="Kamei">Ken-Ichiro Kamei</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Koyama, Yoshie" sort="Koyama, Yoshie" uniqKey="Koyama Y" first="Yoshie" last="Koyama">Yoshie Koyama</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Tokunaga, Yumie" sort="Tokunaga, Yumie" uniqKey="Tokunaga Y" first="Yumie" last="Tokunaga">Yumie Tokunaga</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mashimo, Yasumasa" sort="Mashimo, Yasumasa" uniqKey="Mashimo Y" first="Yasumasa" last="Mashimo">Yasumasa Mashimo</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Yoshioka, Momoko" sort="Yoshioka, Momoko" uniqKey="Yoshioka M" first="Momoko" last="Yoshioka">Momoko Yoshioka</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Fockenberg, Christopher" sort="Fockenberg, Christopher" uniqKey="Fockenberg C" first="Christopher" last="Fockenberg">Christopher Fockenberg</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mosbergen, Rowland" sort="Mosbergen, Rowland" uniqKey="Mosbergen R" first="Rowland" last="Mosbergen">Rowland Mosbergen</name><affiliation wicri:level="1"><nlm:affiliation>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072</wicri:regionArea><wicri:noRegion>4072</wicri:noRegion></affiliation></author><author><name sortKey="Korn, Othmar" sort="Korn, Othmar" uniqKey="Korn O" first="Othmar" last="Korn">Othmar Korn</name><affiliation wicri:level="1"><nlm:affiliation>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072</wicri:regionArea><wicri:noRegion>4072</wicri:noRegion></affiliation></author><author><name sortKey="Wells, Christine" sort="Wells, Christine" uniqKey="Wells C" first="Christine" last="Wells">Christine Wells</name><affiliation wicri:level="1"><nlm:affiliation>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australia Institute for Biotechnology and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, 4072</wicri:regionArea><wicri:noRegion>4072</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yong" sort="Chen, Yong" uniqKey="Chen Y" first="Yong" last="Chen">Yong Chen</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 6068501</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></analytic><series><title level="j">Advanced healthcare materials</title><idno type="eISSN">2192-2659</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human pluripotent stem cells hold great promise for applications in drug discovery and regenerative medicine. Microfluidic technology is a promising approach for creating artificial microenvironments; however, although a proper 3D microenvironment is required to achieve robust control of cellular phenotypes, most current microfluidic devices provide only 2D cell culture and do not allow tuning of physical and chemical environmental cues simultaneously. Here, the authors report a 3D cellular microenvironment plate (3D-CEP), which consists of a microfluidic device filled with thermoresponsive poly(N-isopropylacrylamide)-β-poly(ethylene glycol) hydrogel (HG), which enables systematic tuning of both chemical and physical environmental cues as well as in situ cell monitoring. The authors show that H9 human embryonic stem cells (hESCs) and 253G1 human induced pluripotent stem cells in the HG/3D-CEP system maintain their pluripotent marker expression under HG/3D-CEP self-renewing conditions. Additionally, global gene expression analyses are used to elucidate small variations among different test environments. Interestingly, the authors find that treatment of H9 hESCs under HG/3D-CEP self-renewing conditions results in initiation of entry into the neural differentiation process by induction of PAX3 and OTX1 expression. The authors believe that this HG/3D-CEP system will serve as a versatile platform for developing targeted functional cell lines and facilitate advances in drug screening and regenerative medicine.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002240 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002240 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:27775225
|texte= Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:27775225" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |