Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence.
Identifieur interne : 002084 ( Main/Curation ); précédent : 002083; suivant : 002085Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence.
Auteurs : Daria Chrobok ; Simon R. Law ; Bastiaan Brouwer ; Pernilla Lindén ; Agnieszka Ziolkowska ; Daniela Liebsch ; Reena Narsai ; Bozena Szal ; Thomas Moritz ; Nicolas Rouhier ; James Whelan ; Per Gardeström ; Olivier Keech [Suède]Source :
- Plant physiology [ 1532-2548 ] ; 2016.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Arabidopsis (croissance et développement), Arabidopsis (génétique), Arabidopsis (métabolisme), Feuilles de plante (croissance et développement), Feuilles de plante (métabolisme), Feuilles de plante (ultrastructure), Gènes de mitochondrie, Gènes de plante, Mitochondries (métabolisme), Mitochondries (ultrastructure), Métabolisme énergétique, Métabolomique, Protéines mitochondriales (métabolisme), Respiration cellulaire, Régulation de l'expression des gènes végétaux, Transcription génétique, Transcriptome (génétique), Voies et réseaux métaboliques (génétique).
- MESH :
- croissance et développement : Arabidopsis, Feuilles de plante.
- génétique : Arabidopsis, Transcriptome, Voies et réseaux métaboliques.
- métabolisme : Arabidopsis, Feuilles de plante, Mitochondries, Protéines mitochondriales.
- ultrastructure : Analyse de profil d'expression de gènes, Feuilles de plante, Gènes de mitochondrie, Gènes de plante, Mitochondries, Métabolisme énergétique, Métabolomique, Respiration cellulaire, Régulation de l'expression des gènes végétaux, Transcription génétique.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis (metabolism), Cell Respiration, Energy Metabolism, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Mitochondrial, Genes, Plant, Metabolic Networks and Pathways (genetics), Metabolomics, Mitochondria (metabolism), Mitochondria (ultrastructure), Mitochondrial Proteins (metabolism), Plant Leaves (growth & development), Plant Leaves (metabolism), Plant Leaves (ultrastructure), Transcription, Genetic, Transcriptome (genetics).
- MESH :
- chemical , metabolism : Mitochondrial Proteins.
- genetics : Arabidopsis, Metabolic Networks and Pathways, Transcriptome.
- growth & development : Arabidopsis, Plant Leaves.
- metabolism : Arabidopsis, Mitochondria, Plant Leaves.
- ultrastructure : Mitochondria, Plant Leaves.
- Cell Respiration, Energy Metabolism, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Mitochondrial, Genes, Plant, Metabolomics, Transcription, Genetic.
Abstract
The functions of mitochondria during leaf senescence, a type of programmed cell death aimed at the massive retrieval of nutrients from the senescing organ to the rest of the plant, remain elusive. Here, combining experimental and analytical approaches, we showed that mitochondrial integrity in Arabidopsis (Arabidopsis thaliana) is conserved until the latest stages of leaf senescence, while their number drops by 30%. Adenylate phosphorylation state assays and mitochondrial respiratory measurements indicated that the leaf energy status also is maintained during this time period. Furthermore, after establishing a curated list of genes coding for products targeted to mitochondria, we analyzed in isolation their transcript profiles, focusing on several key mitochondrial functions, such as the tricarboxylic acid cycle, mitochondrial electron transfer chain, iron-sulfur cluster biosynthesis, transporters, as well as catabolic pathways. In tandem with a metabolomic approach, our data indicated that mitochondrial metabolism was reorganized to support the selective catabolism of both amino acids and fatty acids. Such adjustments would ensure the replenishment of α-ketoglutarate and glutamate, which provide the carbon backbones for nitrogen remobilization. Glutamate, being the substrate of the strongly up-regulated cytosolic glutamine synthase, is likely to become a metabolically limiting factor in the latest stages of developmental leaf senescence. Finally, an evolutionary age analysis revealed that, while branched-chain amino acid and proline catabolism are very old mitochondrial functions particularly enriched at the latest stages of leaf senescence, auxin metabolism appears to be rather newly acquired. In summation, our work shows that, during developmental leaf senescence, mitochondria orchestrate catabolic processes by becoming increasingly central energy and metabolic hubs.
DOI: 10.1104/pp.16.01463
PubMed: 27744300
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Daria Chrobok<affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation>Le document en format XML
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Law</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Brouwer, Bastiaan" sort="Brouwer, Bastiaan" uniqKey="Brouwer B" first="Bastiaan" last="Brouwer">Bastiaan Brouwer</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Linden, Pernilla" sort="Linden, Pernilla" uniqKey="Linden P" first="Pernilla" last="Lindén">Pernilla Lindén</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Ziolkowska, Agnieszka" sort="Ziolkowska, Agnieszka" uniqKey="Ziolkowska A" first="Agnieszka" last="Ziolkowska">Agnieszka Ziolkowska</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Liebsch, Daniela" sort="Liebsch, Daniela" uniqKey="Liebsch D" first="Daniela" last="Liebsch">Daniela Liebsch</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Narsai, Reena" sort="Narsai, Reena" uniqKey="Narsai R" first="Reena" last="Narsai">Reena Narsai</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Szal, Bozena" sort="Szal, Bozena" uniqKey="Szal B" first="Bozena" last="Szal">Bozena Szal</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Moritz, Thomas" sort="Moritz, Thomas" uniqKey="Moritz T" first="Thomas" last="Moritz">Thomas Moritz</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Whelan, James" sort="Whelan, James" uniqKey="Whelan J" first="James" last="Whelan">James Whelan</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Gardestrom, Per" sort="Gardestrom, Per" uniqKey="Gardestrom P" first="Per" last="Gardeström">Per Gardeström</name><affiliation><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.).</nlm:affiliation><wicri:noCountry code="subField">O.K.).</wicri:noCountry></affiliation></author><author><name sortKey="Keech, Olivier" sort="Keech, Olivier" uniqKey="Keech O" first="Olivier" last="Keech">Olivier Keech</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-90187 Umea, Sweden (D.C., S.R.L., B.B., A.Z., D.L., P.G., O.K.); olivier.keech@umu.se.</nlm:affiliation><country wicri:rule="url">Suède</country></affiliation></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Arabidopsis (growth & development)</term><term>Arabidopsis (metabolism)</term><term>Cell Respiration</term><term>Energy Metabolism</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genes, Mitochondrial</term><term>Genes, Plant</term><term>Metabolic Networks and Pathways (genetics)</term><term>Metabolomics</term><term>Mitochondria (metabolism)</term><term>Mitochondria (ultrastructure)</term><term>Mitochondrial Proteins (metabolism)</term><term>Plant Leaves (growth & development)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (ultrastructure)</term><term>Transcription, Genetic</term><term>Transcriptome (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Arabidopsis (croissance et développement)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Feuilles de plante (croissance et développement)</term><term>Feuilles de plante (métabolisme)</term><term>Feuilles de plante (ultrastructure)</term><term>Gènes de mitochondrie</term><term>Gènes de plante</term><term>Mitochondries (métabolisme)</term><term>Mitochondries (ultrastructure)</term><term>Métabolisme énergétique</term><term>Métabolomique</term><term>Protéines mitochondriales (métabolisme)</term><term>Respiration cellulaire</term><term>Régulation de l'expression des gènes végétaux</term><term>Transcription génétique</term><term>Transcriptome (génétique)</term><term>Voies et réseaux métaboliques (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mitochondrial Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arabidopsis</term><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Metabolic Networks and Pathways</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Transcriptome</term><term>Voies et réseaux métaboliques</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Mitochondria</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Feuilles de plante</term><term>Mitochondries</term><term>Protéines mitochondriales</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Mitochondria</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Respiration</term><term>Energy Metabolism</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genes, Mitochondrial</term><term>Genes, Plant</term><term>Metabolomics</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Feuilles de plante</term><term>Gènes de mitochondrie</term><term>Gènes de plante</term><term>Mitochondries</term><term>Métabolisme énergétique</term><term>Métabolomique</term><term>Respiration cellulaire</term><term>Régulation de l'expression des gènes végétaux</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The functions of mitochondria during leaf senescence, a type of programmed cell death aimed at the massive retrieval of nutrients from the senescing organ to the rest of the plant, remain elusive. Here, combining experimental and analytical approaches, we showed that mitochondrial integrity in Arabidopsis (Arabidopsis thaliana) is conserved until the latest stages of leaf senescence, while their number drops by 30%. Adenylate phosphorylation state assays and mitochondrial respiratory measurements indicated that the leaf energy status also is maintained during this time period. Furthermore, after establishing a curated list of genes coding for products targeted to mitochondria, we analyzed in isolation their transcript profiles, focusing on several key mitochondrial functions, such as the tricarboxylic acid cycle, mitochondrial electron transfer chain, iron-sulfur cluster biosynthesis, transporters, as well as catabolic pathways. In tandem with a metabolomic approach, our data indicated that mitochondrial metabolism was reorganized to support the selective catabolism of both amino acids and fatty acids. Such adjustments would ensure the replenishment of α-ketoglutarate and glutamate, which provide the carbon backbones for nitrogen remobilization. Glutamate, being the substrate of the strongly up-regulated cytosolic glutamine synthase, is likely to become a metabolically limiting factor in the latest stages of developmental leaf senescence. Finally, an evolutionary age analysis revealed that, while branched-chain amino acid and proline catabolism are very old mitochondrial functions particularly enriched at the latest stages of leaf senescence, auxin metabolism appears to be rather newly acquired. In summation, our work shows that, during developmental leaf senescence, mitochondria orchestrate catabolic processes by becoming increasingly central energy and metabolic hubs.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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