Induction of alternative oxidase by excess copper in sycamore cell suspensions
Identifieur interne : 002C54 ( Istex/Corpus ); précédent : 002C53; suivant : 002C55Induction of alternative oxidase by excess copper in sycamore cell suspensions
Auteurs : Mário Pádua ; Serge Aubert ; Adalcina Casimiro ; Richard Bligny ; A. Harvey Millar ; David A. DaySource :
- Plant Physiology and Biochemistry [ 0981-9428 ] ; 1999.
English descriptors
- KwdEn :
- Acer pseudoplatanus, Active oxygen species, Alternative oxidase, Appropriate cofactors, Ascorbate oxidase, Aubert, Biochem, Bligny, Carbon starvation, Cell growth, Cell respiration, Cell suspensions, Citrate, Constant supply, Consumption rates, Control cells, Copper, Copper treatment, Culture medium, Deuce, Diamine oxidase, Excess copper, Growth medium, Higher plants, Intracellular concentration, Iron uptake, Malate, Mitochondrial, Mitochondrial biogenesis, Mitochondrial protein, Mitochondrial respiration, Mitochondrion, Monoclonal antibodies, Mrna, Nmol, Nmol protein, Northern blot, Northern blot analysis, Open symbols, Other hand, Oxidase, Oxidase induction, Oxygen uptake, Perchloric extracts, Physiol, Plant cell, Plant physiol, Possible mechanisms, Protein levels, Reactive oxygen intermediates, Respiration, Respiratory chain, Respiratory rates, Solid symbols, Sucrose, Sycamore, Sycamore cell respiration, Sycamore cell suspensions, Sycamore cells, Uninhibited cell respiration, Uninhibited respiration, Uptake.
- Teeft :
- Acer pseudoplatanus, Active oxygen species, Alternative oxidase, Appropriate cofactors, Ascorbate oxidase, Aubert, Biochem, Bligny, Carbon starvation, Cell growth, Cell respiration, Cell suspensions, Citrate, Constant supply, Consumption rates, Control cells, Copper, Copper treatment, Culture medium, Deuce, Diamine oxidase, Excess copper, Growth medium, Higher plants, Intracellular concentration, Iron uptake, Malate, Mitochondrial, Mitochondrial biogenesis, Mitochondrial protein, Mitochondrial respiration, Mitochondrion, Monoclonal antibodies, Mrna, Nmol, Nmol protein, Northern blot, Northern blot analysis, Open symbols, Other hand, Oxidase, Oxidase induction, Oxygen uptake, Perchloric extracts, Physiol, Plant cell, Plant physiol, Possible mechanisms, Protein levels, Reactive oxygen intermediates, Respiration, Respiratory chain, Respiratory rates, Solid symbols, Sucrose, Sycamore, Sycamore cell respiration, Sycamore cell suspensions, Sycamore cells, Uninhibited cell respiration, Uninhibited respiration, Uptake.
Abstract
Abstract: Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.
Url:
DOI: 10.1016/S0981-9428(99)80074-6
Links to Exploration step
ISTEX:EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61Le document en format XML
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Pádua was recipient of a scholarship from the Portuguese Government JNICT PRAXIS XXI BD/5276/95 and of a grant from the ‘Ambassade de France’ in Lisbon.</mods:affiliation></affiliation></author><author><name sortKey="Aubert, Serge" sort="Aubert, Serge" uniqKey="Aubert S" first="Serge" last="Aubert">Serge Aubert</name><affiliation><mods:affiliation>Author to whom correspondence should be addressed (fax +33 476885091)</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: aubert@dsvgre.cea.fr</mods:affiliation></affiliation></author><author><name sortKey="Casimiro, Adalcina" sort="Casimiro, Adalcina" uniqKey="Casimiro A" first="Adalcina" last="Casimiro">Adalcina Casimiro</name><affiliation><mods:affiliation>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Bligny, Richard" sort="Bligny, Richard" uniqKey="Bligny R" first="Richard" last="Bligny">Richard Bligny</name><affiliation><mods:affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</mods:affiliation></affiliation></author><author><name sortKey="Millar, A Harvey" sort="Millar, A Harvey" uniqKey="Millar A" first="A. 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Pádua was recipient of a scholarship from the Portuguese Government JNICT PRAXIS XXI BD/5276/95 and of a grant from the ‘Ambassade de France’ in Lisbon.</mods:affiliation></affiliation></author><author><name sortKey="Aubert, Serge" sort="Aubert, Serge" uniqKey="Aubert S" first="Serge" last="Aubert">Serge Aubert</name><affiliation><mods:affiliation>Author to whom correspondence should be addressed (fax +33 476885091)</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: aubert@dsvgre.cea.fr</mods:affiliation></affiliation></author><author><name sortKey="Casimiro, Adalcina" sort="Casimiro, Adalcina" uniqKey="Casimiro A" first="Adalcina" last="Casimiro">Adalcina Casimiro</name><affiliation><mods:affiliation>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Bligny, Richard" sort="Bligny, Richard" uniqKey="Bligny R" first="Richard" last="Bligny">Richard Bligny</name><affiliation><mods:affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</mods:affiliation></affiliation></author><author><name sortKey="Millar, A Harvey" sort="Millar, A Harvey" uniqKey="Millar A" first="A. Harvey" last="Millar">A. Harvey Millar</name><affiliation><mods:affiliation>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</mods:affiliation></affiliation></author><author><name sortKey="Day, David A" sort="Day, David A" uniqKey="Day D" first="David A." last="Day">David A. Day</name><affiliation><mods:affiliation>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Physiology and Biochemistry</title><title level="j" type="abbrev">PLAPHY</title><idno type="ISSN">0981-9428</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="131">131</biblScope><biblScope unit="page" to="137">137</biblScope></imprint><idno type="ISSN">0981-9428</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0981-9428</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acer pseudoplatanus</term><term>Active oxygen species</term><term>Alternative oxidase</term><term>Appropriate cofactors</term><term>Ascorbate oxidase</term><term>Aubert</term><term>Biochem</term><term>Bligny</term><term>Carbon starvation</term><term>Cell growth</term><term>Cell respiration</term><term>Cell suspensions</term><term>Citrate</term><term>Constant supply</term><term>Consumption rates</term><term>Control cells</term><term>Copper</term><term>Copper treatment</term><term>Culture medium</term><term>Deuce</term><term>Diamine oxidase</term><term>Excess copper</term><term>Growth medium</term><term>Higher plants</term><term>Intracellular concentration</term><term>Iron uptake</term><term>Malate</term><term>Mitochondrial</term><term>Mitochondrial biogenesis</term><term>Mitochondrial protein</term><term>Mitochondrial respiration</term><term>Mitochondrion</term><term>Monoclonal antibodies</term><term>Mrna</term><term>Nmol</term><term>Nmol protein</term><term>Northern blot</term><term>Northern blot analysis</term><term>Open symbols</term><term>Other hand</term><term>Oxidase</term><term>Oxidase induction</term><term>Oxygen uptake</term><term>Perchloric extracts</term><term>Physiol</term><term>Plant cell</term><term>Plant physiol</term><term>Possible mechanisms</term><term>Protein levels</term><term>Reactive oxygen intermediates</term><term>Respiration</term><term>Respiratory chain</term><term>Respiratory rates</term><term>Solid symbols</term><term>Sucrose</term><term>Sycamore</term><term>Sycamore cell respiration</term><term>Sycamore cell suspensions</term><term>Sycamore cells</term><term>Uninhibited cell respiration</term><term>Uninhibited respiration</term><term>Uptake</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acer pseudoplatanus</term><term>Active oxygen species</term><term>Alternative oxidase</term><term>Appropriate cofactors</term><term>Ascorbate oxidase</term><term>Aubert</term><term>Biochem</term><term>Bligny</term><term>Carbon starvation</term><term>Cell growth</term><term>Cell respiration</term><term>Cell suspensions</term><term>Citrate</term><term>Constant supply</term><term>Consumption rates</term><term>Control cells</term><term>Copper</term><term>Copper treatment</term><term>Culture medium</term><term>Deuce</term><term>Diamine oxidase</term><term>Excess copper</term><term>Growth medium</term><term>Higher plants</term><term>Intracellular concentration</term><term>Iron uptake</term><term>Malate</term><term>Mitochondrial</term><term>Mitochondrial biogenesis</term><term>Mitochondrial protein</term><term>Mitochondrial respiration</term><term>Mitochondrion</term><term>Monoclonal antibodies</term><term>Mrna</term><term>Nmol</term><term>Nmol protein</term><term>Northern blot</term><term>Northern blot analysis</term><term>Open symbols</term><term>Other hand</term><term>Oxidase</term><term>Oxidase induction</term><term>Oxygen uptake</term><term>Perchloric extracts</term><term>Physiol</term><term>Plant cell</term><term>Plant physiol</term><term>Possible mechanisms</term><term>Protein levels</term><term>Reactive oxygen intermediates</term><term>Respiration</term><term>Respiratory chain</term><term>Respiratory rates</term><term>Solid symbols</term><term>Sucrose</term><term>Sycamore</term><term>Sycamore cell respiration</term><term>Sycamore cell suspensions</term><term>Sycamore cells</term><term>Uninhibited cell respiration</term><term>Uninhibited respiration</term><term>Uptake</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>sycamore</json:string><json:string>mitochondrion</json:string><json:string>oxidase</json:string><json:string>physiol</json:string><json:string>control cells</json:string><json:string>sycamore cells</json:string><json:string>plant physiol</json:string><json:string>mitochondrial</json:string><json:string>malate</json:string><json:string>citrate</json:string><json:string>deuce</json:string><json:string>mrna</json:string><json:string>nmol</json:string><json:string>aubert</json:string><json:string>copper treatment</json:string><json:string>sucrose</json:string><json:string>respiration</json:string><json:string>excess copper</json:string><json:string>bligny</json:string><json:string>biochem</json:string><json:string>culture medium</json:string><json:string>alternative oxidase</json:string><json:string>nmol protein</json:string><json:string>copper</json:string><json:string>sycamore cell suspensions</json:string><json:string>oxygen uptake</json:string><json:string>uninhibited respiration</json:string><json:string>other hand</json:string><json:string>reactive oxygen intermediates</json:string><json:string>monoclonal antibodies</json:string><json:string>growth medium</json:string><json:string>appropriate cofactors</json:string><json:string>consumption rates</json:string><json:string>northern blot</json:string><json:string>mitochondrial protein</json:string><json:string>acer pseudoplatanus</json:string><json:string>uptake</json:string><json:string>uninhibited cell respiration</json:string><json:string>respiratory rates</json:string><json:string>ascorbate oxidase</json:string><json:string>constant supply</json:string><json:string>iron uptake</json:string><json:string>respiratory chain</json:string><json:string>mitochondrial respiration</json:string><json:string>plant cell</json:string><json:string>mitochondrial biogenesis</json:string><json:string>oxidase induction</json:string><json:string>carbon starvation</json:string><json:string>cell growth</json:string><json:string>cell respiration</json:string><json:string>protein levels</json:string><json:string>open symbols</json:string><json:string>active oxygen species</json:string><json:string>intracellular concentration</json:string><json:string>perchloric extracts</json:string><json:string>higher plants</json:string><json:string>diamine oxidase</json:string><json:string>possible mechanisms</json:string><json:string>cell suspensions</json:string><json:string>sycamore cell respiration</json:string><json:string>northern blot analysis</json:string><json:string>solid symbols</json:string></teeft></keywords><author><json:item><name>Mário Pádua</name><affiliations><json:string>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</json:string><json:string>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</json:string><json:string>§ M. Pádua was recipient of a scholarship from the Portuguese Government JNICT PRAXIS XXI BD/5276/95 and of a grant from the ‘Ambassade de France’ in Lisbon.</json:string></affiliations></json:item><json:item><name>Serge Aubert</name><affiliations><json:string>Author to whom correspondence should be addressed (fax +33 476885091)</json:string><json:string>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</json:string><json:string>E-mail: aubert@dsvgre.cea.fr</json:string></affiliations></json:item><json:item><name>Adalcina Casimiro</name><affiliations><json:string>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>Richard Bligny</name><affiliations><json:string>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</json:string></affiliations></json:item><json:item><name>A.Harvey Millar</name><affiliations><json:string>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</json:string></affiliations></json:item><json:item><name>David A. Day</name><affiliations><json:string>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Acer pseudoplatanus / alternative oxidase / copper / plant metabolism / respiration</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>AOX</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>alternative oxidase / GABA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>γ-aminobutyric acid / KCN</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>potassium cyanide / SHAM</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>salicylhydroxamic acid</value></json:item></subject><arkIstex>ark:/67375/6H6-JMZF0XV8-B</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.</abstract><qualityIndicators><score>7.246</score><pdfWordCount>3926</pdfWordCount><pdfCharCount>24867</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>594 x 774 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>110</abstractWordCount><abstractCharCount>777</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Induction of alternative oxidase by excess copper in sycamore cell suspensions</title><pii><json:string>S0981-9428(99)80074-6</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Plant Physiology and Biochemistry</title><language><json:string>unknown</json:string></language><publicationDate>1999</publicationDate><issn><json:string>0981-9428</json:string></issn><pii><json:string>S0981-9428(00)X0044-7</json:string></pii><volume>37</volume><issue>2</issue><pages><first>131</first><last>137</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1999</json:string><json:string>7, 5-6 and 23</json:string></date><geogName></geogName><orgName><json:string>Division of Biochemistry Australia</json:string><json:string>Universidade National University, Canberra ACT</json:string><json:string>Hansatech Ltd</json:string><json:string>Australian Research Council</json:string><json:string>Biochem</json:string><json:string>‘Ambas</json:string></orgName><orgName_funder><json:string>‘Ambas</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Dr E. Gout</json:string><json:string>Paris Acer</json:string><json:string>A.H. Millar</json:string><json:string>D.A. Day</json:string><json:string>I. Effects</json:string><json:string>David A. Da</json:string></persName><placeName><json:string>Grenoble</json:string><json:string>UK</json:string><json:string>Norfolk</json:string><json:string>Lisbon</json:string><json:string>Portugal</json:string><json:string>France</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[4]</json:string><json:string>[23]</json:string><json:string>[29]</json:string><json:string>[6]</json:string><json:string>[33]</json:string><json:string>[28]</json:string><json:string>Whelan et al. [35]</json:string><json:string>[32]</json:string><json:string>[3]</json:string><json:string>[20]</json:string><json:string>Millar et al. [21]</json:string><json:string>[5]</json:string><json:string>[26]</json:string><json:string>Minagawa et al. [22]</json:string><json:string>Aubert et al. [3]</json:string><json:string>Aubert et al. [2]</json:string><json:string>[30]</json:string><json:string>[7]</json:string><json:string>Sambrook et al. [27]</json:string><json:string>[25]</json:string><json:string>[30,33]</json:string><json:string>[9]</json:string><json:string>[24]</json:string><json:string>Lowry et al.</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-JMZF0XV8-B</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - plant sciences</json:string></wos><scienceMetrix><json:string>1 - natural sciences</json:string><json:string>2 - biology</json:string><json:string>3 - plant biology & botany</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Agricultural and Biological Sciences</json:string><json:string>3 - Plant Science</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Genetics</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Physiology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - psychologie. psychophysiologie</json:string></inist></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0981-9428(99)80074-6</json:string></doi><id>EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Induction of alternative oxidase by excess copper in sycamore cell suspensions</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1999</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Induction of alternative oxidase by excess copper in sycamore cell suspensions</title><author xml:id="author-0000"><persName><forename type="first">Mário</forename><surname>Pádua</surname></persName><affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</affiliation><affiliation>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</affiliation><affiliation>§ M. Pádua was recipient of a scholarship from the Portuguese Government JNICT PRAXIS XXI BD/5276/95 and of a grant from the ‘Ambassade de France’ in Lisbon.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Serge</forename><surname>Aubert</surname></persName><email>aubert@dsvgre.cea.fr</email><affiliation>Author to whom correspondence should be addressed (fax +33 476885091)</affiliation><affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Adalcina</forename><surname>Casimiro</surname></persName><affiliation>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Richard</forename><surname>Bligny</surname></persName><affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">A.Harvey</forename><surname>Millar</surname></persName><affiliation>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</affiliation></author><author xml:id="author-0005"><persName><forename type="first">David A.</forename><surname>Day</surname></persName><affiliation>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</affiliation></author><idno type="istex">EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61</idno><idno type="DOI">10.1016/S0981-9428(99)80074-6</idno><idno type="PII">S0981-9428(99)80074-6</idno></analytic><monogr><title level="j">Plant Physiology and Biochemistry</title><title level="j" type="abbrev">PLAPHY</title><idno type="pISSN">0981-9428</idno><idno type="PII">S0981-9428(00)X0044-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">37</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="131">131</biblScope><biblScope unit="page" to="137">137</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Acer pseudoplatanus / alternative oxidase / copper / plant metabolism / respiration</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>AOX</term></item><item><term>alternative oxidase / GABA</term></item><item><term>γ-aminobutyric acid / KCN</term></item><item><term>potassium cyanide / SHAM</term></item><item><term>salicylhydroxamic acid</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>PLAPHY</jid><aid>99800746</aid><ce:pii>S0981-9428(99)80074-6</ce:pii><ce:doi>10.1016/S0981-9428(99)80074-6</ce:doi><ce:copyright type="unknown" year="1999"></ce:copyright></item-info><head><ce:title>Induction of alternative oxidase by excess copper in sycamore cell suspensions</ce:title><ce:author-group><ce:author><ce:given-name>Mário</ce:given-name><ce:surname>Pádua</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>§</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Serge</ce:given-name><ce:surname>Aubert</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:e-address>aubert@dsvgre.cea.fr</ce:e-address></ce:author><ce:author><ce:given-name>Adalcina</ce:given-name><ce:surname>Casimiro</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Richard</ce:given-name><ce:surname>Bligny</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.Harvey</ce:given-name><ce:surname>Millar</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>David A.</ce:given-name><ce:surname>Day</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Author to whom correspondence should be addressed (fax +33 476885091)</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>§</ce:label><ce:note-para>M. Pádua was recipient of a scholarship from the Portuguese Government JNICT PRAXIS XXI BD/5276/95 and of a grant from the ‘Ambassade de France’ in Lisbon.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="4" month="9" year="1998"></ce:date-received><ce:date-accepted day="12" month="11" year="1998"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Sycamore suspension cells (<ce:italic>Acer pseudoplatanus</ce:italic> L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb <ce:italic>AOX</ce:italic> mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Acer pseudoplatanus</ce:italic> / alternative oxidase / copper / plant metabolism / respiration</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>AOX</ce:text></ce:keyword><ce:keyword><ce:text>alternative oxidase / GABA</ce:text></ce:keyword><ce:keyword><ce:text>γ-aminobutyric acid / KCN</ce:text></ce:keyword><ce:keyword><ce:text>potassium cyanide / SHAM</ce:text></ce:keyword><ce:keyword><ce:text>salicylhydroxamic acid</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Induction of alternative oxidase by excess copper in sycamore cell suspensions</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Induction of alternative oxidase by excess copper in sycamore cell suspensions</title></titleInfo><name type="personal"><namePart type="given">Mário</namePart><namePart type="family">Pádua</namePart><affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</affiliation><affiliation>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</affiliation><affiliation>§ M. Pádua was recipient of a scholarship from the Portuguese Government JNICT PRAXIS XXI BD/5276/95 and of a grant from the ‘Ambassade de France’ in Lisbon.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Serge</namePart><namePart type="family">Aubert</namePart><affiliation>Author to whom correspondence should be addressed (fax +33 476885091)</affiliation><affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</affiliation><affiliation>E-mail: aubert@dsvgre.cea.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Adalcina</namePart><namePart type="family">Casimiro</namePart><affiliation>Laboratório de Fisiologia e Bioquímica Vegetais, Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C-2, Campo Grande, 1700 Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Richard</namePart><namePart type="family">Bligny</namePart><affiliation>Laboratoire de Physiologie Cellulaire Végétale, DBMS, CEA (CNRS URA 576), 17, rue des Martyrs, 38054 Grenoble cedex 9, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.Harvey</namePart><namePart type="family">Millar</namePart><affiliation>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David A.</namePart><namePart type="family">Day</namePart><affiliation>Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Sycamore suspension cells (Acer pseudoplatanus L.) were used to investigate the effect of copper on respiratory electron transport. Alternative oxidase (AOX) protein content and enzymatic capacity increased as a function of the concentration of copper added to the culture medium, from 0.2 to 50 μM. The latter sublethal concentration, which arrests mitochondrial biogenesis and thereby decreases cell respiration rates, stimulated cyanide-insensitive oxygen uptake in cells and mitochondria isolated therefrom. This was correlated with the accumulation of two proteins (30 and 36 kDa) which reacted with monoclonal antibodies against AOX. An accumulation of a 1.6-kb AOX mRNA was also observed. The possible mechanisms through which copper affects AOX are discussed.</abstract><subject><genre>Keywords</genre><topic>Acer pseudoplatanus / alternative oxidase / copper / plant metabolism / respiration</topic></subject><subject><genre>Abbreviations</genre><topic>AOX</topic><topic>alternative oxidase / GABA</topic><topic>γ-aminobutyric acid / KCN</topic><topic>potassium cyanide / SHAM</topic><topic>salicylhydroxamic acid</topic></subject><relatedItem type="host"><titleInfo><title>Plant Physiology and Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>PLAPHY</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199902</dateIssued></originInfo><identifier type="ISSN">0981-9428</identifier><identifier type="PII">S0981-9428(00)X0044-7</identifier><part><date>199902</date><detail type="volume"><number>37</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>87</start><end>165</end></extent><extent unit="pages"><start>131</start><end>137</end></extent></part></relatedItem><identifier type="istex">EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61</identifier><identifier type="ark">ark:/67375/6H6-JMZF0XV8-B</identifier><identifier type="DOI">10.1016/S0981-9428(99)80074-6</identifier><identifier type="PII">S0981-9428(99)80074-6</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/EC0D08C43FC8FF108E5C42CCC7F5B57C33BDBD61/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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