Oligodeoxynucleotides as probes for in situ hybridization with transmission electron microscopy to specifically localize phytoplasma in plant cells
Identifieur interne : 002969 ( Istex/Corpus ); précédent : 002968; suivant : 002970Oligodeoxynucleotides as probes for in situ hybridization with transmission electron microscopy to specifically localize phytoplasma in plant cells
Auteurs : J. Lherminier ; R. G. Bonfiglioli ; X. Daire ; R. H. Symons ; E. Boudon-PadieuSource :
- Molecular and Cellular Probes [ 0890-8508 ] ; 1999.
English descriptors
- KwdEn :
- Academic press, Catharanthus roseus, Complementary sequences, Cultivar differences, Denaturing treatment, Dijon cedex, Doree, Electron micrographs, Electron microscopy, Experimental conditions, Flavescence, Flavescence doree, Formamide concentrations, Gold beads, Gold labelling, Gold particles, Gold resin, Grapevine, Grapevine yellows, Grid, Host plant, Hybridization, Hybridization buffer, Hybridization mixture, Hybridization signal, Labelling, Light label, Molecular cytology, Nucleic acids, Oligodeoxynucleotides, Periwinkle, Phloem, Phloem elements, Phytoplasma, Phytoplasma cells, Phytoplasma detection, Phytoplasmas, Pipes buffer, Plant cells, Plant disease, Plant tissue, Polymerase chain reaction, Probe, Rdna, Room temperature, Same plant species, Secondary structure, Senescent forms, Sense probe, Sequence analysis, Several groups, Stolbur, Stolbur group, Stolbur phytoplasma, Stringency conditions, Successive treatments, Target sequences, Tissue sections, Transmission electron microscopy, Unrelated probe, Yellows group, in situhybridization, transmission electron microscopy, rDNA, oligoprobes, gold labelling..
- Teeft :
- Academic press, Catharanthus roseus, Complementary sequences, Cultivar differences, Denaturing treatment, Dijon cedex, Doree, Electron micrographs, Electron microscopy, Experimental conditions, Flavescence, Flavescence doree, Formamide concentrations, Gold beads, Gold labelling, Gold particles, Gold resin, Grapevine, Grapevine yellows, Grid, Host plant, Hybridization, Hybridization buffer, Hybridization mixture, Hybridization signal, Labelling, Light label, Molecular cytology, Nucleic acids, Oligodeoxynucleotides, Periwinkle, Phloem, Phloem elements, Phytoplasma, Phytoplasma cells, Phytoplasma detection, Phytoplasmas, Pipes buffer, Plant cells, Plant disease, Plant tissue, Polymerase chain reaction, Probe, Rdna, Room temperature, Same plant species, Secondary structure, Senescent forms, Sense probe, Sequence analysis, Several groups, Stolbur, Stolbur group, Stolbur phytoplasma, Stringency conditions, Successive treatments, Target sequences, Tissue sections, Transmission electron microscopy, Unrelated probe, Yellows group.
Abstract
Abstract: Phytoplasmas are plant-pathogenic mollicutes restricted to phloem. They belong to several groups in a unique phylogenetic clade. Non-related phytoplasmas may infect the same plant species, often with similar symptoms. Hence methods are needed to specifically localize phytoplasmas and to study their multiplication and movement in their hosts. Conditions for post-embeddingin situhybridization (ISH) with transmission electron microscopy using oligodeoxynucleotides as probes for labelling of phytoplasmas in plant tissues have been searched. Sections of acrylic resin-embedded tissues of phytoplasma-infected periwinkle were submitted to ISH using digoxigenin or biotin-labelled oligoprobes (22 mers). These probes were the complementary sequences of primers used in group-specific polymerase chain reaction (PCR) amplification of 16S rDNA of stolbur and elm yellows phytoplasma, respectively. Together with preliminary digestion with pepsin, differentin situdenaturation conditions and formamide concentrations were tested. The grids were incubated in the hybridization mixture at 37°C overnight. Detection of hybridized material was performed with gold immunocytochemistry. Specificity of labelling was checked with appropriate controls. Stringency conditions could be found to ensure specific hybridization with such short probes. A specific labelling was obtained for stolbur phytoplasma on groups of mature as well as senescent phytoplasma cells. The results show that oligonucleotides may be used as probes for phytoplasma identification in post-embedding ISH with electron microscopy.
Url:
DOI: 10.1006/mcpr.1998.0213
Links to Exploration step
ISTEX:DEC79778BCBF8DF514AD2BAF95754B2968A1779FLe document en format XML
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They belong to several groups in a unique phylogenetic clade. Non-related phytoplasmas may infect the same plant species, often with similar symptoms. Hence methods are needed to specifically localize phytoplasmas and to study their multiplication and movement in their hosts. Conditions for post-embeddingin situhybridization (ISH) with transmission electron microscopy using oligodeoxynucleotides as probes for labelling of phytoplasmas in plant tissues have been searched. Sections of acrylic resin-embedded tissues of phytoplasma-infected periwinkle were submitted to ISH using digoxigenin or biotin-labelled oligoprobes (22 mers). These probes were the complementary sequences of primers used in group-specific polymerase chain reaction (PCR) amplification of 16S rDNA of stolbur and elm yellows phytoplasma, respectively. Together with preliminary digestion with pepsin, differentin situdenaturation conditions and formamide concentrations were tested. The grids were incubated in the hybridization mixture at 37°C overnight. Detection of hybridized material was performed with gold immunocytochemistry. Specificity of labelling was checked with appropriate controls. Stringency conditions could be found to ensure specific hybridization with such short probes. A specific labelling was obtained for stolbur phytoplasma on groups of mature as well as senescent phytoplasma cells. The results show that oligonucleotides may be used as probes for phytoplasma identification in post-embedding ISH with electron microscopy.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>hybridization</json:string><json:string>phytoplasmas</json:string><json:string>phytoplasma</json:string><json:string>labelling</json:string><json:string>stolbur</json:string><json:string>grid</json:string><json:string>rdna</json:string><json:string>periwinkle</json:string><json:string>flavescence</json:string><json:string>electron microscopy</json:string><json:string>oligodeoxynucleotides</json:string><json:string>doree</json:string><json:string>hybridization mixture</json:string><json:string>gold beads</json:string><json:string>tissue sections</json:string><json:string>transmission electron microscopy</json:string><json:string>flavescence doree</json:string><json:string>phytoplasma cells</json:string><json:string>stolbur phytoplasma</json:string><json:string>hybridization buffer</json:string><json:string>pipes buffer</json:string><json:string>stringency conditions</json:string><json:string>phloem</json:string><json:string>gold particles</json:string><json:string>academic press</json:string><json:string>stolbur group</json:string><json:string>hybridization signal</json:string><json:string>grapevine yellows</json:string><json:string>plant cells</json:string><json:string>dijon cedex</json:string><json:string>gold labelling</json:string><json:string>room temperature</json:string><json:string>plant tissue</json:string><json:string>grapevine</json:string><json:string>probe</json:string><json:string>experimental conditions</json:string><json:string>sequence analysis</json:string><json:string>phytoplasma detection</json:string><json:string>gold resin</json:string><json:string>cultivar differences</json:string><json:string>host plant</json:string><json:string>unrelated probe</json:string><json:string>sense probe</json:string><json:string>formamide concentrations</json:string><json:string>polymerase chain reaction</json:string><json:string>nucleic acids</json:string><json:string>successive treatments</json:string><json:string>denaturing treatment</json:string><json:string>yellows group</json:string><json:string>complementary sequences</json:string><json:string>electron micrographs</json:string><json:string>catharanthus roseus</json:string><json:string>phloem elements</json:string><json:string>same plant species</json:string><json:string>senescent forms</json:string><json:string>light label</json:string><json:string>several groups</json:string><json:string>secondary structure</json:string><json:string>target sequences</json:string><json:string>plant disease</json:string><json:string>molecular cytology</json:string></teeft></keywords><author><json:item><name>J. 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Conditions for post-embeddingin situhybridization (ISH) with transmission electron microscopy using oligodeoxynucleotides as probes for labelling of phytoplasmas in plant tissues have been searched. Sections of acrylic resin-embedded tissues of phytoplasma-infected periwinkle were submitted to ISH using digoxigenin or biotin-labelled oligoprobes (22 mers). These probes were the complementary sequences of primers used in group-specific polymerase chain reaction (PCR) amplification of 16S rDNA of stolbur and elm yellows phytoplasma, respectively. Together with preliminary digestion with pepsin, differentin situdenaturation conditions and formamide concentrations were tested. The grids were incubated in the hybridization mixture at 37°C overnight. Detection of hybridized material was performed with gold immunocytochemistry. Specificity of labelling was checked with appropriate controls. Stringency conditions could be found to ensure specific hybridization with such short probes. A specific labelling was obtained for stolbur phytoplasma on groups of mature as well as senescent phytoplasma cells. 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sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - phytopathologie. zoologie agricole. protection des cultures et des forets</json:string></inist></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1006/mcpr.1998.0213</json:string></doi><id>DEC79778BCBF8DF514AD2BAF95754B2968A1779F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/DEC79778BCBF8DF514AD2BAF95754B2968A1779F/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/DEC79778BCBF8DF514AD2BAF95754B2968A1779F/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/DEC79778BCBF8DF514AD2BAF95754B2968A1779F/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Oligodeoxynucleotides as probes for in situ hybridization with transmission electron microscopy to specifically localize phytoplasma in plant cells</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Academic Press</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Oligodeoxynucleotides as probes for in situ hybridization with transmission electron microscopy to specifically localize phytoplasma in plant cells</title><author xml:id="author-0000"><persName><forename type="first">J.</forename><surname>Lherminier</surname></persName><affiliation>Service Commun de Microscopie, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">R.G.</forename><surname>Bonfiglioli</surname></persName><affiliation>CRC for Viticulture, Department of Plant Science, Waite Institute, University of Adelaide, Glen Osmond, 5064, SA, Australia</affiliation></author><author xml:id="author-0002"><persName><forename type="first">X.</forename><surname>Daire</surname></persName><affiliation>Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">R.H.</forename><surname>Symons</surname></persName><affiliation>CRC for Viticulture, Department of Plant Science, Waite Institute, University of Adelaide, Glen Osmond, 5064, SA, Australia</affiliation></author><author xml:id="author-0004"><persName><forename type="first">E.</forename><surname>Boudon-Padieu</surname></persName><affiliation>Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</affiliation></author><idno type="istex">DEC79778BCBF8DF514AD2BAF95754B2968A1779F</idno><idno type="DOI">10.1006/mcpr.1998.0213</idno><idno type="PII">S0890-8508(98)90213-4</idno></analytic><monogr><title level="j">Molecular and Cellular Probes</title><title level="j" type="abbrev">YMCPR</title><idno type="pISSN">0890-8508</idno><idno type="PII">S0890-8508(00)X0012-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">13</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="41">41</biblScope><biblScope unit="page" to="47">47</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Phytoplasmas are plant-pathogenic mollicutes restricted to phloem. They belong to several groups in a unique phylogenetic clade. Non-related phytoplasmas may infect the same plant species, often with similar symptoms. Hence methods are needed to specifically localize phytoplasmas and to study their multiplication and movement in their hosts. Conditions for post-embeddingin situhybridization (ISH) with transmission electron microscopy using oligodeoxynucleotides as probes for labelling of phytoplasmas in plant tissues have been searched. Sections of acrylic resin-embedded tissues of phytoplasma-infected periwinkle were submitted to ISH using digoxigenin or biotin-labelled oligoprobes (22 mers). These probes were the complementary sequences of primers used in group-specific polymerase chain reaction (PCR) amplification of 16S rDNA of stolbur and elm yellows phytoplasma, respectively. Together with preliminary digestion with pepsin, differentin situdenaturation conditions and formamide concentrations were tested. The grids were incubated in the hybridization mixture at 37°C overnight. Detection of hybridized material was performed with gold immunocytochemistry. Specificity of labelling was checked with appropriate controls. Stringency conditions could be found to ensure specific hybridization with such short probes. A specific labelling was obtained for stolbur phytoplasma on groups of mature as well as senescent phytoplasma cells. The results show that oligonucleotides may be used as probes for phytoplasma identification in post-embedding ISH with electron microscopy.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>in situhybridization, transmission electron microscopy, rDNA, oligoprobes, gold labelling.</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-09-30">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/DEC79778BCBF8DF514AD2BAF95754B2968A1779F/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YMCPR</jid><aid>90213</aid><ce:pii>S0890-8508(98)90213-4</ce:pii><ce:doi>10.1006/mcpr.1998.0213</ce:doi><ce:copyright type="full-transfer" year="1999">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Oligodeoxynucleotides as probes for<ce:bold><ce:italic>in situ</ce:italic></ce:bold>hybridization with transmission electron microscopy to specifically localize phytoplasma in plant cells</ce:title><ce:author-group><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Lherminier</ce:surname><ce:cross-ref refid="QA1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.G.</ce:given-name><ce:surname>Bonfiglioli</ce:surname><ce:cross-ref refid="QA2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>X.</ce:given-name><ce:surname>Daire</ce:surname><ce:cross-ref refid="QA3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.H.</ce:given-name><ce:surname>Symons</ce:surname><ce:cross-ref refid="QA2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Boudon-Padieu</ce:surname><ce:cross-ref refid="QA3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="QFN1"><ce:sup>f1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="QA1"><ce:label>a</ce:label><ce:textfn>Service Commun de Microscopie, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</ce:textfn></ce:affiliation><ce:affiliation id="QA2"><ce:label>b</ce:label><ce:textfn>CRC for Viticulture, Department of Plant Science, Waite Institute, University of Adelaide, Glen Osmond, 5064, SA, Australia</ce:textfn></ce:affiliation><ce:affiliation id="QA3"><ce:label>c</ce:label><ce:textfn>Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</ce:textfn></ce:affiliation><ce:footnote id="QFN1"><ce:label>f1</ce:label><ce:note-para>Author to whom all correspondence should be addressed at: Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034 Dijon Cedex, France. Tel: +33 3 80 63 31 91; Fax: +33 3 80 63 32 61; E-mail: boudon@epoisses.inra.fr</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="25" month="2" year="1998"></ce:date-received><ce:date-accepted day="29" month="9" year="1998"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para id="simple-para0005">Phytoplasmas are plant-pathogenic mollicutes restricted to phloem. They belong to several groups in a unique phylogenetic clade. Non-related phytoplasmas may infect the same plant species, often with similar symptoms. Hence methods are needed to specifically localize phytoplasmas and to study their multiplication and movement in their hosts. Conditions for post-embedding<ce:italic>in situ</ce:italic>hybridization (ISH) with transmission electron microscopy using oligodeoxynucleotides as probes for labelling of phytoplasmas in plant tissues have been searched. Sections of acrylic resin-embedded tissues of phytoplasma-infected periwinkle were submitted to ISH using digoxigenin or biotin-labelled oligoprobes (22 mers). These probes were the complementary sequences of primers used in group-specific polymerase chain reaction (PCR) amplification of 16S rDNA of stolbur and elm yellows phytoplasma, respectively. Together with preliminary digestion with pepsin, different<ce:italic>in situ</ce:italic>denaturation conditions and formamide concentrations were tested. The grids were incubated in the hybridization mixture at 37°C overnight. Detection of hybridized material was performed with gold immunocytochemistry. Specificity of labelling was checked with appropriate controls. Stringency conditions could be found to ensure specific hybridization with such short probes. A specific labelling was obtained for stolbur phytoplasma on groups of mature as well as senescent phytoplasma cells. The results show that oligonucleotides may be used as probes for phytoplasma identification in post-embedding ISH with electron microscopy.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>in situ</ce:italic>hybridization, transmission electron microscopy, rDNA, oligoprobes, gold labelling.</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Oligodeoxynucleotides as probes for in situ hybridization with transmission electron microscopy to specifically localize phytoplasma in plant cells</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Oligodeoxynucleotides as probes for</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Lherminier</namePart><affiliation>Service Commun de Microscopie, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.G.</namePart><namePart type="family">Bonfiglioli</namePart><affiliation>CRC for Viticulture, Department of Plant Science, Waite Institute, University of Adelaide, Glen Osmond, 5064, SA, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X.</namePart><namePart type="family">Daire</namePart><affiliation>Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.H.</namePart><namePart type="family">Symons</namePart><affiliation>CRC for Viticulture, Department of Plant Science, Waite Institute, University of Adelaide, Glen Osmond, 5064, SA, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Boudon-Padieu</namePart><affiliation>Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034, Dijon Cedex, France</affiliation><description>Author to whom all correspondence should be addressed at: Recherches sur les Phytoplasmes, Laboratoire de Phytoparasitologie, INRA, BV 1540, 21034 Dijon Cedex, France. Tel: +33 3 80 63 31 91; Fax: +33 3 80 63 32 61; E-mail: boudon@epoisses.inra.fr</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Phytoplasmas are plant-pathogenic mollicutes restricted to phloem. They belong to several groups in a unique phylogenetic clade. Non-related phytoplasmas may infect the same plant species, often with similar symptoms. Hence methods are needed to specifically localize phytoplasmas and to study their multiplication and movement in their hosts. Conditions for post-embeddingin situhybridization (ISH) with transmission electron microscopy using oligodeoxynucleotides as probes for labelling of phytoplasmas in plant tissues have been searched. Sections of acrylic resin-embedded tissues of phytoplasma-infected periwinkle were submitted to ISH using digoxigenin or biotin-labelled oligoprobes (22 mers). These probes were the complementary sequences of primers used in group-specific polymerase chain reaction (PCR) amplification of 16S rDNA of stolbur and elm yellows phytoplasma, respectively. Together with preliminary digestion with pepsin, differentin situdenaturation conditions and formamide concentrations were tested. The grids were incubated in the hybridization mixture at 37°C overnight. Detection of hybridized material was performed with gold immunocytochemistry. Specificity of labelling was checked with appropriate controls. Stringency conditions could be found to ensure specific hybridization with such short probes. A specific labelling was obtained for stolbur phytoplasma on groups of mature as well as senescent phytoplasma cells. The results show that oligonucleotides may be used as probes for phytoplasma identification in post-embedding ISH with electron microscopy.</abstract><note type="content">Section title: Regular Article</note><subject lang="en"><genre>Keywords</genre><topic>in situhybridization, transmission electron microscopy, rDNA, oligoprobes, gold labelling.</topic></subject><relatedItem type="host"><titleInfo><title>Molecular and Cellular Probes</title></titleInfo><titleInfo type="abbreviated"><title>YMCPR</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199902</dateIssued></originInfo><identifier type="ISSN">0890-8508</identifier><identifier type="PII">S0890-8508(00)X0012-6</identifier><part><date>199902</date><detail type="volume"><number>13</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>79</end></extent><extent unit="pages"><start>41</start><end>47</end></extent></part></relatedItem><identifier type="istex">DEC79778BCBF8DF514AD2BAF95754B2968A1779F</identifier><identifier type="ark">ark:/67375/6H6-9PPJ3DXH-Q</identifier><identifier type="DOI">10.1006/mcpr.1998.0213</identifier><identifier type="PII">S0890-8508(98)90213-4</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1999</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/DEC79778BCBF8DF514AD2BAF95754B2968A1779F/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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