AustralieFrV1, Istex, Corpus, bibRecord, 000A89

Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils

Identifieur interne : 000A89 ( Istex/Corpus ); précédent : 000A88; suivant : 000A90

Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils

Auteurs : C. Joulian ; B. Ollivier ; B. K. C. Patel ; P. A. Roger

Source :

FEMS Microbiology Ecology [ 0168-6496 ] ; 1998.

RBID : ISTEX:3A3BEF664974D107516C0C9EB22C4C941DB65722

English descriptors

Abstract

Abstract: Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from <10 to 104 g−1 d.w. on acetate, from <10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.

Url:

https://api.istex.fr/document/3A3BEF664974D107516C0C9EB22C4C941DB65722/fulltext/pdf

DOI: 10.1016/S0168-6496(97)00090-1

Links to Exploration step

ISTEX:3A3BEF664974D107516C0C9EB22C4C941DB65722

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from <10 to 104 g−1 d.w. on acetate, from <10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.</div>
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<abstract>Abstract: Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from >10 to 104 g−1 d.w. on acetate, from >10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.</abstract>
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<notesStmt><note type="content">Fig. 1: Restriction profiles of the isolated rods. Mb. bryantii group included strains RiH2, H2Cal3, TAH2, PBH2, PDH2, CBH2, LuiH2, MaH2, H2Sol2, SelH2, GMH2, MaliH2, PAH2, IAH2 and PiH2; Mb. formicicum group included strains FCam, FSol1 and TiaH2. L, ladder; a, BamHI; b, CfoI; c, Sau3A; d, TaqI.</note>
<note type="content">Fig. 2: Dendrogram showing the position of the methanogenic strains isolated from ricefields among representative methanogens of various origins.</note>
<note type="content">Table 1: Primers used for amplification and sequencing of 16S rRNA gene of methanogens</note>
<note type="content">Table 2: Counts of major trophic groups of methanogens</note>
<note type="content">Table 3: Origin, morphology, and energy sources of isolated strains</note>
<note type="content">Table 4: Evolutionary similarity matrix for methanogens from ricefields and various methanogens</note>
<note type="content">Table 5: Major characteristics of the isolated strains and related speciesa</note>
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<author xml:id="author-0002"><persName><forename type="first">B.K.C.</forename>
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<abstract xml:lang="en"><p>Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from <10 to 104 g−1 d.w. on acetate, from <10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.</p>
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<head><ce:title>Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils</ce:title>
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<ce:abstract-sec><ce:simple-para>Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 10<ce:sup>2</ce:sup>
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 on methanol, and from 50 to 10<ce:sup>6</ce:sup>
 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H<ce:inf>2</ce:inf>
, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to <ce:italic>Methanobacterium bryantii</ce:italic>
; three hydrogenotrophic-formatotrophic rods affiliated to <ce:italic>Mb. formicicum</ce:italic>
; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to <ce:italic>Methanosarcina barkeri</ce:italic>
 and <ce:italic>Methanosarcina mazei;</ce:italic>
 and one irregular coccus affiliated to <ce:italic>Methanoculleus marisnigri</ce:italic>
 – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of <ce:italic>Methanobacterium</ce:italic>
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<ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title>
<ce:keyword><ce:text>Methane</ce:text>
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<ce:keyword><ce:text>Methanogen</ce:text>
</ce:keyword>
<ce:keyword><ce:text>MPN count</ce:text>
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<ce:keyword><ce:text>Ricefield</ce:text>
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<ce:keyword><ce:text>Soil</ce:text>
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<ce:keyword><ce:text>Phylogeny</ce:text>
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<abstract lang="en">Abstract: Populations of the four major trophic groups of methanogens were enumerated by most probable numbers (MPN) on selective media in a sample of 13 soils representative of major types of rice soils. Dominant strains were isolated and their phenotypic and phylogenetic characteristics were studied. MPN counts ranged from 102 to 106 g−1 d.w. on H2, from <10 to 104 g−1 d.w. on acetate, from <10 to 105 on methanol, and from 50 to 106 on formate. In most soils, counts of hydrogenotrophs were higher than counts of acetotrophs, partly because acetotrophs were aggregated sarcinae difficult to separate into individual cells. Methylotrophs other than acetotrophic sarcinae were not recorded. In most soils, rods enumerated on formate were 5–400 times less abundant than those enumerated on H2, indicating that hydrogenotrophic-non-formatotrophic rods are probably dominant in ricefields. Dominant strains isolated comprised: 15 hydrogenotrophic-non-formatotrophic rods affiliated to Methanobacterium bryantii; three hydrogenotrophic-formatotrophic rods affiliated to Mb. formicicum; one hydrogenotrophic-formatotrophic rod not affiliated to a sequenced species; two sarcinae affiliated to Methanosarcina barkeri and Methanosarcina mazei; and one irregular coccus affiliated to Methanoculleus marisnigri – a species so far isolated from marine sediments only. Results from classical counts of methanogens and strains isolated from ricefields suggest the dominance of Methanobacterium spp. (mostly responsible for CH4 production from H2/CO2) and Methanosarcina spp. (mostly responsible for CH4 production from acetate) among culturable organisms. Both genera are probably ubiquitous. In particular, Mb. bryantii was isolated from 12 of the 13 soils.</abstract>
<note type="content">Fig. 1: Restriction profiles of the isolated rods. Mb. bryantii group included strains RiH2, H2Cal3, TAH2, PBH2, PDH2, CBH2, LuiH2, MaH2, H2Sol2, SelH2, GMH2, MaliH2, PAH2, IAH2 and PiH2; Mb. formicicum group included strains FCam, FSol1 and TiaH2. L, ladder; a, BamHI; b, CfoI; c, Sau3A; d, TaqI.</note>
<note type="content">Fig. 2: Dendrogram showing the position of the methanogenic strains isolated from ricefields among representative methanogens of various origins.</note>
<note type="content">Table 1: Primers used for amplification and sequencing of 16S rRNA gene of methanogens</note>
<note type="content">Table 2: Counts of major trophic groups of methanogens</note>
<note type="content">Table 3: Origin, morphology, and energy sources of isolated strains</note>
<note type="content">Table 4: Evolutionary similarity matrix for methanogens from ricefields and various methanogens</note>
<note type="content">Table 5: Major characteristics of the isolated strains and related speciesa</note>
<subject><genre>Keywords</genre>
<topic>Methane</topic>
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<topic>MPN count</topic>
<topic>Ricefield</topic>
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<topic>Phylogeny</topic>
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Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils

Phenotypic and phylogenetic characterization of dominant culturable methanogens isolated from ricefield soils

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