Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume
Identifieur interne : 000986 ( Istex/Checkpoint ); précédent : 000985; suivant : 000987Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume
Auteurs : Linda Tabe [Australie] ; Markus Wirtz [Allemagne] ; Lisa Molvig [Australie] ; Michel Droux [France] ; Ruediger Hell [Allemagne]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2009-11-25.
Abstract
There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosyntheis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F2 seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein.
Url:
DOI: 10.1093/jxb/erp338
Affiliations:
- Allemagne, Australie, France
- Auvergne-Rhône-Alpes, Bade-Wurtemberg, District de Karlsruhe, Rhône-Alpes
- Heidelberg, Lyon
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IV: Molekulare Biologie der Pflanzen, Im Neuenheimer Feld 360, D-69120 Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-11-25">2009-11-25</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="721">721</biblScope><biblScope unit="page" to="733">733</biblScope></imprint><idno type="ISSN">0022-0957</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosyntheis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F2 seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li><li>Rhône-Alpes</li></region><settlement><li>Heidelberg</li><li>Lyon</li></settlement></list><tree><country name="Australie"><noRegion><name sortKey="Tabe, Linda" sort="Tabe, Linda" uniqKey="Tabe L" first="Linda" last="Tabe">Linda Tabe</name></noRegion><name sortKey="Molvig, Lisa" sort="Molvig, Lisa" uniqKey="Molvig L" first="Lisa" last="Molvig">Lisa Molvig</name><name sortKey="Tabe, Linda" sort="Tabe, Linda" uniqKey="Tabe L" first="Linda" last="Tabe">Linda Tabe</name><name sortKey="Tabe, Linda" sort="Tabe, Linda" uniqKey="Tabe L" first="Linda" last="Tabe">Linda Tabe</name></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Wirtz, Markus" sort="Wirtz, Markus" uniqKey="Wirtz M" first="Markus" last="Wirtz">Markus Wirtz</name></region><name sortKey="Hell, Ruediger" sort="Hell, Ruediger" uniqKey="Hell R" first="Ruediger" last="Hell">Ruediger Hell</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Droux, Michel" sort="Droux, Michel" uniqKey="Droux M" first="Michel" last="Droux">Michel Droux</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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