Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
Identifieur interne : 000C52 ( Istex/Corpus ); précédent : 000C51; suivant : 000C53Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
Auteurs : David R. Russell ; George N. BennettSource :
- Gene [ 0378-1119 ] ; 1981.
Abstract
A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.
Url:
DOI: 10.1016/0378-1119(82)90096-8
Links to Exploration step
ISTEX:7B3E2C0AC7EACC67BF7C383593CF127FE124EB89Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title>
<author><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name>
<affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name>
<affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno>
<date when="1982" year="1982">1982</date>
<idno type="doi">10.1016/0378-1119(82)90096-8</idno>
<idno type="url">https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C52</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title>
<author><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name>
<affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name>
<affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Gene</title>
<title level="j" type="abbrev">GENE</title>
<idno type="ISSN">0378-1119</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1981">1981</date>
<biblScope unit="volume">17</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="9">9</biblScope>
<biblScope unit="page" to="18">18</biblScope>
</imprint>
<idno type="ISSN">0378-1119</idno>
</series>
<idno type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno>
<idno type="DOI">10.1016/0378-1119(82)90096-8</idno>
<idno type="PII">0378-1119(82)90096-8</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0378-1119</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</div>
</front>
</TEI>
<istex><corpusName>elsevier</corpusName>
<author><json:item><name>David R. Russell</name>
<affiliations><json:string>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</json:string>
</affiliations>
</json:item>
<json:item><name>George N. Bennett</name>
<affiliations><json:string>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</json:string>
</affiliations>
</json:item>
</author>
<subject><json:item><lang><json:string>eng</json:string>
</lang>
<value>Recombinant DNA</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>trp represser</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>oligo promoter-operators</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>transcription control</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>ACH : acid casein hydrolysate</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>bp : base pairs</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>KmR : kanamycin resistance</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>5MT : 5-methyltryptophan</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>TcR : tetracycline resistance</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Δ : deletion</value>
</json:item>
</subject>
<language><json:string>eng</json:string>
</language>
<abstract>A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</abstract>
<qualityIndicators><score>6.758</score>
<pdfVersion>1.4</pdfVersion>
<pdfPageSize>540 x 756 pts</pdfPageSize>
<refBibsNative>true</refBibsNative>
<keywordCount>10</keywordCount>
<abstractCharCount>982</abstractCharCount>
<pdfWordCount>4482</pdfWordCount>
<pdfCharCount>30826</pdfCharCount>
<pdfPageCount>10</pdfPageCount>
<abstractWordCount>148</abstractWordCount>
</qualityIndicators>
<title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title>
<pii><json:string>0378-1119(82)90096-8</json:string>
</pii>
<genre><json:string>research-article</json:string>
</genre>
<serie><volume>Vol. 17</volume>
<pages><last>380</last>
<first>365</first>
</pages>
<genre></genre>
<language><json:string>unknown</json:string>
</language>
<title>Methods in Enzymology</title>
</serie>
<host><volume>17</volume>
<pii><json:string>S0378-1119(00)X0306-X</json:string>
</pii>
<pages><last>18</last>
<first>9</first>
</pages>
<issn><json:string>0378-1119</json:string>
</issn>
<issue>1</issue>
<genre><json:string>Journal</json:string>
</genre>
<language><json:string>unknown</json:string>
</language>
<title>Gene</title>
<publicationDate>1982</publicationDate>
</host>
<categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string>
<json:string>GENETICS & HEREDITY</json:string>
</wos>
</categories>
<publicationDate>1981</publicationDate>
<copyrightDate>1982</copyrightDate>
<doi><json:string>10.1016/0378-1119(82)90096-8</json:string>
</doi>
<id>7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</id>
<fulltext><json:item><original>true</original>
<mimetype>application/pdf</mimetype>
<extension>pdf</extension>
<uri>https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/pdf</uri>
</json:item>
<json:item><original>true</original>
<mimetype>text/plain</mimetype>
<extension>txt</extension>
<uri>https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/txt</uri>
</json:item>
<json:item><original>false</original>
<mimetype>application/zip</mimetype>
<extension>zip</extension>
<uri>https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher>ELSEVIER</publisher>
<availability><p>ELSEVIER</p>
</availability>
<date>1982</date>
</publicationStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title>
<author><persName><forename type="first">David R.</forename>
<surname>Russell</surname>
</persName>
<affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation>
</author>
<author><persName><forename type="first">George N.</forename>
<surname>Bennett</surname>
</persName>
<affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation>
</author>
</analytic>
<monogr><title level="j">Gene</title>
<title level="j" type="abbrev">GENE</title>
<idno type="pISSN">0378-1119</idno>
<idno type="PII">S0378-1119(00)X0306-X</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1981"></date>
<biblScope unit="volume">17</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="9">9</biblScope>
<biblScope unit="page" to="18">18</biblScope>
</imprint>
</monogr>
<idno type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno>
<idno type="DOI">10.1016/0378-1119(82)90096-8</idno>
<idno type="PII">0378-1119(82)90096-8</idno>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1982</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</p>
</abstract>
<textClass><keywords scheme="keyword"><list><head>Keywords</head>
<item><term>Recombinant DNA</term>
</item>
<item><term>trp represser</term>
</item>
<item><term>oligo promoter-operators</term>
</item>
<item><term>transcription control</term>
</item>
</list>
</keywords>
</textClass>
<textClass><keywords scheme="keyword"><list><head>Abbreviations</head>
<item><term>ACH</term>
<term>acid casein hydrolysate</term>
</item>
<item><term>bp</term>
<term>base pairs</term>
</item>
<item><term>KmR</term>
<term>kanamycin resistance</term>
</item>
<item><term>5MT</term>
<term>5-methyltryptophan</term>
</item>
<item><term>TcR</term>
<term>tetracycline resistance</term>
</item>
<item><term>Δ</term>
<term>deletion</term>
</item>
</list>
</keywords>
</textClass>
</profileDesc>
<revisionDesc><change when="1981-08-19">Registration</change>
<change when="1981">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType>
<istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>GENE</jid>
<aid>82900968</aid>
<ce:pii>0378-1119(82)90096-8</ce:pii>
<ce:doi>10.1016/0378-1119(82)90096-8</ce:doi>
<ce:copyright type="unknown" year="1982"></ce:copyright>
</item-info>
<head><ce:title>Cloning of small DNA fragments containing the <ce:italic>Escherichia coli</ce:italic>
tryptophan operon promoter and operator</ce:title>
<ce:author-group><ce:author><ce:given-name>David R.</ce:given-name>
<ce:surname>Russell</ce:surname>
</ce:author>
<ce:author><ce:given-name>George N.</ce:given-name>
<ce:surname>Bennett</ce:surname>
</ce:author>
<ce:affiliation><ce:textfn>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</ce:textfn>
</ce:affiliation>
</ce:author-group>
<ce:date-received day="5" month="6" year="1981"></ce:date-received>
<ce:date-accepted day="19" month="8" year="1981"></ce:date-accepted>
<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para>A 41-bp <ce:italic>Alu</ce:italic>
I restriction fragment from the <ce:italic>trp</ce:italic>
promoter-operator region has been cloned into the <ce:italic>Pvu</ce:italic>
II site of pBR322, regenerating <ce:italic>Pvu</ce:italic>
II sites. Transformants were selected on media that allowed the selection of <ce:italic>trp</ce:italic>
-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by <ce:italic>Pvu</ce:italic>
II digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: <ce:italic>Hin</ce:italic>
cII, <ce:italic>Taq</ce:italic>
I, <ce:italic>Rsa</ce:italic>
I, and a <ce:italic>Hpa</ce:italic>
I site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the <ce:italic>Hpa</ce:italic>
I site, were prepared from the 41-bp fragment by joining two right-side, or two left-side <ce:italic>Pvu</ce:italic>
II-<ce:italic>Hpa</ce:italic>
I pieces together at the <ce:italic>Hpa</ce:italic>
I site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp <ce:italic>trp</ce:italic>
-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
<ce:keywords><ce:section-title>Keywords</ce:section-title>
<ce:keyword><ce:text>Recombinant DNA</ce:text>
</ce:keyword>
<ce:keyword><ce:text><ce:italic>trp</ce:italic>
represser</ce:text>
</ce:keyword>
<ce:keyword><ce:text>oligo promoter-operators</ce:text>
</ce:keyword>
<ce:keyword><ce:text>transcription control</ce:text>
</ce:keyword>
</ce:keywords>
<ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title>
<ce:keyword><ce:text>ACH</ce:text>
<ce:keyword><ce:text>acid casein hydrolysate</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>bp</ce:text>
<ce:keyword><ce:text>base pairs</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>Km<ce:sup>R</ce:sup>
</ce:text>
<ce:keyword><ce:text>kanamycin resistance</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>5MT</ce:text>
<ce:keyword><ce:text>5-methyltryptophan</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>Tc<ce:sup>R</ce:sup>
</ce:text>
<ce:keyword><ce:text>tetracycline resistance</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>Δ</ce:text>
<ce:keyword><ce:text>deletion</ce:text>
</ce:keyword>
</ce:keyword>
</ce:keywords>
</head>
</converted-article>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo><title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Cloning of small DNA fragments containing the</title>
</titleInfo>
<name type="personal"><namePart type="given">David R.</namePart>
<namePart type="family">Russell</namePart>
<affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">George N.</namePart>
<namePart type="family">Bennett</namePart>
<affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="Full-length article"></genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1981</dateIssued>
<dateValid encoding="w3cdtf">1981-08-19</dateValid>
<copyrightDate encoding="w3cdtf">1982</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="iso639-2b">eng</languageTerm>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
</language>
<physicalDescription><internetMediaType>text/html</internetMediaType>
</physicalDescription>
<abstract lang="en">A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</abstract>
<subject><genre>Keywords</genre>
<topic>Recombinant DNA</topic>
<topic>trp represser</topic>
<topic>oligo promoter-operators</topic>
<topic>transcription control</topic>
</subject>
<subject><genre>Abbreviations</genre>
<topic>ACH : acid casein hydrolysate</topic>
<topic>bp : base pairs</topic>
<topic>KmR : kanamycin resistance</topic>
<topic>5MT : 5-methyltryptophan</topic>
<topic>TcR : tetracycline resistance</topic>
<topic>Δ : deletion</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Gene</title>
</titleInfo>
<titleInfo type="abbreviated"><title>GENE</title>
</titleInfo>
<genre type="Journal">journal</genre>
<originInfo><dateIssued encoding="w3cdtf">198201</dateIssued>
</originInfo>
<identifier type="ISSN">0378-1119</identifier>
<identifier type="PII">S0378-1119(00)X0306-X</identifier>
<part><date>198201</date>
<detail type="volume"><number>17</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>1</number>
<caption>no.</caption>
</detail>
<extent unit="issue pages"><start>1</start>
<end>116</end>
</extent>
<extent unit="pages"><start>9</start>
<end>18</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</identifier>
<identifier type="DOI">10.1016/0378-1119(82)90096-8</identifier>
<identifier type="PII">0378-1119(82)90096-8</identifier>
<recordInfo><recordContentSource>ELSEVIER</recordContentSource>
</recordInfo>
</mods>
</metadata>
<enrichments><istex:catWosTEI uri="https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode>
<classCode scheme="WOS">GENETICS & HEREDITY</classCode>
</textClass>
</profileDesc>
</teiHeader>
</istex:catWosTEI>
</enrichments>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C52 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000C52 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Musique |area= OperaV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:7B3E2C0AC7EACC67BF7C383593CF127FE124EB89 |texte= Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator }}
![]() | This area was generated with Dilib version V0.6.21. | ![]() |