Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
Identifieur interne : 000C52 ( Istex/Corpus ); précédent : 000C51; suivant : 000C53Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
Auteurs : David R. Russell ; George N. BennettSource :
- Gene [ 0378-1119 ] ; 1981.
Abstract
A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.
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DOI: 10.1016/0378-1119(82)90096-8
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title><author><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name><affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name><affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno><date when="1982" year="1982">1982</date><idno type="doi">10.1016/0378-1119(82)90096-8</idno><idno type="url">https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000C52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title><author><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name><affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name><affiliation><mods:affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">17</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="18">18</biblScope></imprint><idno type="ISSN">0378-1119</idno></series><idno type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno><idno type="DOI">10.1016/0378-1119(82)90096-8</idno><idno type="PII">0378-1119(82)90096-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>David R. Russell</name><affiliations><json:string>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</json:string></affiliations></json:item><json:item><name>George N. Bennett</name><affiliations><json:string>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Recombinant DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>trp represser</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>oligo promoter-operators</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>transcription control</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ACH : acid casein hydrolysate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>bp : base pairs</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>KmR : kanamycin resistance</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>5MT : 5-methyltryptophan</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TcR : tetracycline resistance</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Δ : deletion</value></json:item></subject><language><json:string>eng</json:string></language><abstract>A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</abstract><qualityIndicators><score>6.758</score><pdfVersion>1.4</pdfVersion><pdfPageSize>540 x 756 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>10</keywordCount><abstractCharCount>982</abstractCharCount><pdfWordCount>4482</pdfWordCount><pdfCharCount>30826</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>148</abstractWordCount></qualityIndicators><title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title><pii><json:string>0378-1119(82)90096-8</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>Vol. 17</volume><pages><last>380</last><first>365</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Methods in Enzymology</title></serie><host><volume>17</volume><pii><json:string>S0378-1119(00)X0306-X</json:string></pii><pages><last>18</last><first>9</first></pages><issn><json:string>0378-1119</json:string></issn><issue>1</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Gene</title><publicationDate>1982</publicationDate></host><categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string><json:string>GENETICS & HEREDITY</json:string></wos></categories><publicationDate>1981</publicationDate><copyrightDate>1982</copyrightDate><doi><json:string>10.1016/0378-1119(82)90096-8</json:string></doi><id>7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1982</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title><author><persName><forename type="first">David R.</forename><surname>Russell</surname></persName><affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation></author><author><persName><forename type="first">George N.</forename><surname>Bennett</surname></persName><affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation></author></analytic><monogr><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="pISSN">0378-1119</idno><idno type="PII">S0378-1119(00)X0306-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981"></date><biblScope unit="volume">17</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="18">18</biblScope></imprint></monogr><idno type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno><idno type="DOI">10.1016/0378-1119(82)90096-8</idno><idno type="PII">0378-1119(82)90096-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1982</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Recombinant DNA</term></item><item><term>trp represser</term></item><item><term>oligo promoter-operators</term></item><item><term>transcription control</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>ACH</term><term>acid casein hydrolysate</term></item><item><term>bp</term><term>base pairs</term></item><item><term>KmR</term><term>kanamycin resistance</term></item><item><term>5MT</term><term>5-methyltryptophan</term></item><item><term>TcR</term><term>tetracycline resistance</term></item><item><term>Δ</term><term>deletion</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1981-08-19">Registration</change><change when="1981">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>GENE</jid><aid>82900968</aid><ce:pii>0378-1119(82)90096-8</ce:pii><ce:doi>10.1016/0378-1119(82)90096-8</ce:doi><ce:copyright type="unknown" year="1982"></ce:copyright></item-info><head><ce:title>Cloning of small DNA fragments containing the <ce:italic>Escherichia coli</ce:italic> tryptophan operon promoter and operator</ce:title><ce:author-group><ce:author><ce:given-name>David R.</ce:given-name><ce:surname>Russell</ce:surname></ce:author><ce:author><ce:given-name>George N.</ce:given-name><ce:surname>Bennett</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="5" month="6" year="1981"></ce:date-received><ce:date-accepted day="19" month="8" year="1981"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A 41-bp <ce:italic>Alu</ce:italic>I restriction fragment from the <ce:italic>trp</ce:italic> promoter-operator region has been cloned into the <ce:italic>Pvu</ce:italic>II site of pBR322, regenerating <ce:italic>Pvu</ce:italic>II sites. Transformants were selected on media that allowed the selection of <ce:italic>trp</ce:italic>-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by <ce:italic>Pvu</ce:italic>II digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: <ce:italic>Hin</ce:italic>cII, <ce:italic>Taq</ce:italic>I, <ce:italic>Rsa</ce:italic>I, and a <ce:italic>Hpa</ce:italic>I site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the <ce:italic>Hpa</ce:italic>I site, were prepared from the 41-bp fragment by joining two right-side, or two left-side <ce:italic>Pvu</ce:italic>II-<ce:italic>Hpa</ce:italic>I pieces together at the <ce:italic>Hpa</ce:italic>I site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp <ce:italic>trp</ce:italic>-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Recombinant DNA</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>trp</ce:italic> represser</ce:text></ce:keyword><ce:keyword><ce:text>oligo promoter-operators</ce:text></ce:keyword><ce:keyword><ce:text>transcription control</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>ACH</ce:text><ce:keyword><ce:text>acid casein hydrolysate</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>bp</ce:text><ce:keyword><ce:text>base pairs</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Km<ce:sup>R</ce:sup></ce:text><ce:keyword><ce:text>kanamycin resistance</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>5MT</ce:text><ce:keyword><ce:text>5-methyltryptophan</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Tc<ce:sup>R</ce:sup></ce:text><ce:keyword><ce:text>tetracycline resistance</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>Δ</ce:text><ce:keyword><ce:text>deletion</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Cloning of small DNA fragments containing the</title></titleInfo><name type="personal"><namePart type="given">David R.</namePart><namePart type="family">Russell</namePart><affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">George N.</namePart><namePart type="family">Bennett</namePart><affiliation>Department of Biochemistry, Rice University, Houston, TX 77001 U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued><dateValid encoding="w3cdtf">1981-08-19</dateValid><copyrightDate encoding="w3cdtf">1982</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</abstract><subject><genre>Keywords</genre><topic>Recombinant DNA</topic><topic>trp represser</topic><topic>oligo promoter-operators</topic><topic>transcription control</topic></subject><subject><genre>Abbreviations</genre><topic>ACH : acid casein hydrolysate</topic><topic>bp : base pairs</topic><topic>KmR : kanamycin resistance</topic><topic>5MT : 5-methyltryptophan</topic><topic>TcR : tetracycline resistance</topic><topic>Δ : deletion</topic></subject><relatedItem type="host"><titleInfo><title>Gene</title></titleInfo><titleInfo type="abbreviated"><title>GENE</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">198201</dateIssued></originInfo><identifier type="ISSN">0378-1119</identifier><identifier type="PII">S0378-1119(00)X0306-X</identifier><part><date>198201</date><detail type="volume"><number>17</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>116</end></extent><extent unit="pages"><start>9</start><end>18</end></extent></part></relatedItem><identifier type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</identifier><identifier type="DOI">10.1016/0378-1119(82)90096-8</identifier><identifier type="PII">0378-1119(82)90096-8</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode><classCode scheme="WOS">GENETICS & HEREDITY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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