PaleopathV1, Ncbi, Merge, bibRecord, 000983

DNA repair enables sex identification in genetic material from human teeth

Identifieur interne : 000983 ( Ncbi/Merge ); précédent : 000982; suivant : 000984

DNA repair enables sex identification in genetic material from human teeth

Auteurs : L. Kovatsi [Grèce] ; D. Nikou [Grèce] ; S. Triantaphyllou [Royaume-Uni] ; S N Njau [Grèce] ; S. Voutsaki [Pays-Bas] ; S. Kouidou [Grèce]

Source :

Hippokratia [ 1108-4189 ] ; 2009.

RBID : PMC:2765294

Abstract

Background: The purpose of this study was to test the effectiveness of a DNA repair protocol in improving genetic testing in compromised samples, frequently encountered in Forensic Medicine.

Methods: In order to stretch the experiment conditions to the limits, as far as quality of samples and DNA is concerned, we tried the repair protocol on ten ancient human teeth obtained from an equal number of skeletons from a burial site in Lerna, Middle Helladic Greece (2100 - 1700 BC). For these samples, sex was previously determined morphologically, serving as a reference to compare our molecular data with. The samples were analysed using the DNA amelogenin sex test assay prior and after DNA polymerase repair. For every individual, two molecular sex determinations were obtained by visualising PCR products on an agarose gel.

Results: DNA repair enabled genetic testing in these samples. Successful amplification of the amelogenin gene was obtained only from the repaired DNA in eight out of ten samples. Prior to the repair treatment, none of these samples yielded any PCR products, thus attesting to the authenticity of the amplified sequence. The concordance between morphological and molecular analysis was in reasonable agreement (71%).

Conclusions: These results reveal the impact of the repair process in studying single copy genes from low quality DNA. This protocol could facilitate molecular analysis in compromised samples, encountered in forensic medicine, as well as enable genetic studies in ancient remnants.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765294

PubMed: 19918305
PubMed Central: 2765294

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<author><name sortKey="Kovatsi, L" sort="Kovatsi, L" uniqKey="Kovatsi L" first="L" last="Kovatsi">L. Kovatsi</name>
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<series><title level="j">Hippokratia</title>
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<front><div type="abstract" xml:lang="en"><p><bold>Background:</bold>
 The purpose of this study was to test the effectiveness of a DNA repair protocol in improving genetic testing in compromised samples, frequently encountered in Forensic Medicine.</p>
<p><bold>Methods:</bold>
 In order to stretch the experiment conditions to the limits, as far as quality of samples and DNA is concerned, we tried the repair protocol on ten ancient human teeth obtained from an equal number of skeletons from a burial site in Lerna, Middle Helladic Greece (2100 - 1700 BC). For these samples, sex was previously determined morphologically, serving as a reference to compare our molecular data with. The samples were analysed using the DNA amelogenin sex test assay prior and after DNA polymerase repair. For every individual, two molecular sex determinations were obtained by visualising PCR products on an agarose gel.</p>
<p><bold>Results:</bold>
 DNA repair enabled genetic testing in these samples. Successful amplification of the amelogenin gene was obtained only from the repaired DNA in eight out of ten samples. Prior to the repair treatment, none of these samples yielded any PCR products, thus attesting to the authenticity of the amplified sequence. The concordance between morphological and molecular analysis was in reasonable agreement (71%).</p>
<p><bold>Conclusions:</bold>
 These results reveal the impact of the repair process in studying single copy genes from low quality DNA. This protocol could facilitate molecular analysis in compromised samples, encountered in forensic medicine, as well as enable genetic studies in ancient remnants.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Hippokratia</journal-id>
<journal-title-group><journal-title>Hippokratia</journal-title>
</journal-title-group>
<issn pub-type="ppub">1108-4189</issn>
<issn pub-type="epub">1790-8019</issn>
<publisher><publisher-name>LITHOGRAPHIA Antoniadis I.-Psarras Th. G.P.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19918305</article-id>
<article-id pub-id-type="pmc">2765294</article-id>
<article-id pub-id-type="publisher-id">091330165</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>DNA repair enables sex identification in genetic material from human teeth</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kovatsi</surname>
<given-names>L</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nikou</surname>
<given-names>D</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Triantaphyllou</surname>
<given-names>S</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Njau</surname>
<given-names>S N</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Voutsaki</surname>
<given-names>S</given-names>
</name>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kouidou</surname>
<given-names>S</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<aff id="A1"><label>1</label>
Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece</aff>
<aff id="A2"><label>2</label>
Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki, Greece</aff>
<aff id="A3"><label>3</label>
Sheffield Centre for Aegean Archaeology, Department of Archaeology, University of Sheffield, UK</aff>
<aff id="A4"><label>4</label>
Groningen Institute of Archaeology, University of Groningen, The Netherlands</aff>
</contrib-group>
<author-notes><corresp>Kovatsi Leda, Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University, Thessaloniki, 54124, Greece, Tel: +30 2310 999222
				<email>kovatsi@med.auth.gr</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><season>Jul-Sep</season>
<year>2009</year>
</pub-date>
<volume>13</volume>
<issue>3</issue>
<fpage>165</fpage>
<lpage>168</lpage>
<permissions><copyright-statement>Copyright 2009, Hippokratio General Hospital of Thessaloniki</copyright-statement>
<copyright-year>2009</copyright-year>
</permissions>
<abstract><p><bold>Background:</bold>
 The purpose of this study was to test the effectiveness of a DNA repair protocol in improving genetic testing in compromised samples, frequently encountered in Forensic Medicine.</p>
<p><bold>Methods:</bold>
 In order to stretch the experiment conditions to the limits, as far as quality of samples and DNA is concerned, we tried the repair protocol on ten ancient human teeth obtained from an equal number of skeletons from a burial site in Lerna, Middle Helladic Greece (2100 - 1700 BC). For these samples, sex was previously determined morphologically, serving as a reference to compare our molecular data with. The samples were analysed using the DNA amelogenin sex test assay prior and after DNA polymerase repair. For every individual, two molecular sex determinations were obtained by visualising PCR products on an agarose gel.</p>
<p><bold>Results:</bold>
 DNA repair enabled genetic testing in these samples. Successful amplification of the amelogenin gene was obtained only from the repaired DNA in eight out of ten samples. Prior to the repair treatment, none of these samples yielded any PCR products, thus attesting to the authenticity of the amplified sequence. The concordance between morphological and molecular analysis was in reasonable agreement (71%).</p>
<p><bold>Conclusions:</bold>
 These results reveal the impact of the repair process in studying single copy genes from low quality DNA. This protocol could facilitate molecular analysis in compromised samples, encountered in forensic medicine, as well as enable genetic studies in ancient remnants.</p>
</abstract>
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<kwd>forensics</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Grèce</li>
<li>Pays-Bas</li>
<li>Royaume-Uni</li>
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<region><li>Groningue (province)</li>
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<country name="Pays-Bas"><region name="Groningue (province)"><name sortKey="Voutsaki, S" sort="Voutsaki, S" uniqKey="Voutsaki S" first="S" last="Voutsaki">S. Voutsaki</name>
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DNA repair enables sex identification in genetic material from human teeth

DNA repair enables sex identification in genetic material from human teeth

Source :

Abstract

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