PaleopathV1, Ncbi, Checkpoint, bibRecord, 000452

Characterization of Mycobacterium tuberculosis Complex DNAs from Egyptian Mummies by Spoligotyping

Identifieur interne : 000452 ( Ncbi/Checkpoint ); précédent : 000451; suivant : 000453

Characterization of Mycobacterium tuberculosis Complex DNAs from Egyptian Mummies by Spoligotyping

Auteurs : Albert R. Zink ; Christophe Sola ; Udo Reischl ; Waltraud Grabner ; Nalin Rastogi ; Hans Wolf ; Andreas G. Nerlich

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2003.

RBID : PMC:149558

Descripteurs français

Wicri :
- geographic : Égypte.

English descriptors

KwdEn :
- DNA, Bacterial (analysis), Databases, Factual, Egypt, History, Ancient, Humans, Mummies (microbiology), Mycobacterium bovis (isolation & purification), Mycobacterium tuberculosis (isolation & purification), Paleopathology, Polymerase Chain Reaction.
MESH :
- chemical , analysis : DNA, Bacterial.
- geographic : Egypt.
- isolation & purification : Mycobacterium bovis, Mycobacterium tuberculosis.
- microbiology : Mummies.
- Databases, Factual, History, Ancient, Humans, Paleopathology, Polymerase Chain Reaction.

Abstract

Bone and soft tissue samples from 85 ancient Egyptian mummies were analyzed for the presence of ancient Mycobacterium tuberculosis complex DNA (aDNA) and further characterized by spoligotyping. The specimens were obtained from individuals from different tomb complexes in Thebes West, Upper Egypt, which were used for upper social class burials between the Middle Kingdom (since ca. 2050 BC) and the Late Period (until ca. 500 BC). A total of 25 samples provided a specific positive signal for the amplification of a 123-bp fragment of the repetitive element IS6110, indicating the presence of M. tuberculosis DNA. Further PCR-based tests for the identification of subspecies failed due to lack of specific amplification products in the historic tissue samples. Of these 25 positive specimens, 12 could be successfully characterized by spoligotyping. The spoligotyping signatures were compared to those in an international database. They all show either an M. tuberculosis or an M. africanum pattern, but none revealed an M. bovis-specific pattern. The results from a Middle Kingdom tomb (used exclusively between ca. 2050 and 1650 BC) suggest that these samples bear an M. africanum-type specific spoligotyping signature. The samples from later periods provided patterns typical for M. tuberculosis. This study clearly demonstrates that spoligotyping can be applied to historic tissue samples. In addition, our results do not support the theory that M. tuberculosis originated from the M. bovis type but, rather, suggest that human M. tuberculosis may have originated from a precursor complex probably related to M. africanum.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC149558

DOI: 10.1128/JCM.41.1.359-367.2003
PubMed: 12517873
PubMed Central: 149558

Affiliations:

Links to Exploration step

PMC:149558

Le document en format XML

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 Complex DNAs from Egyptian Mummies by Spoligotyping</title>
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<front><div type="abstract" xml:lang="en"><p>Bone and soft tissue samples from 85 ancient Egyptian mummies were analyzed for the presence of ancient <italic>Mycobacterium tuberculosis</italic>
 complex DNA (aDNA) and further characterized by spoligotyping. The specimens were obtained from individuals from different tomb complexes in Thebes West, Upper Egypt, which were used for upper social class burials between the Middle Kingdom (since ca. 2050 BC) and the Late Period (until ca. 500 BC). A total of 25 samples provided a specific positive signal for the amplification of a 123-bp fragment of the repetitive element IS<italic>6110</italic>
, indicating the presence of <italic>M. tuberculosis</italic>
 DNA. Further PCR-based tests for the identification of subspecies failed due to lack of specific amplification products in the historic tissue samples. Of these 25 positive specimens, 12 could be successfully characterized by spoligotyping. The spoligotyping signatures were compared to those in an international database. They all show either an <italic>M. tuberculosis</italic>
 or an <italic>M. africanum</italic>
 pattern, but none revealed an <italic>M. bovis</italic>
-specific pattern. The results from a Middle Kingdom tomb (used exclusively between ca. 2050 and 1650 BC) suggest that these samples bear an <italic>M. africanum</italic>
-type specific spoligotyping signature. The samples from later periods provided patterns typical for <italic>M. tuberculosis</italic>
. This study clearly demonstrates that spoligotyping can be applied to historic tissue samples. In addition, our results do not support the theory that <italic>M. tuberculosis</italic>
 originated from the <italic>M. bovis</italic>
 type but, rather, suggest that human <italic>M. tuberculosis</italic>
 may have originated from a precursor complex probably related to <italic>M. africanum</italic>
.</p>
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<tree><noCountry><name sortKey="Grabner, Waltraud" sort="Grabner, Waltraud" uniqKey="Grabner W" first="Waltraud" last="Grabner">Waltraud Grabner</name>
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<name sortKey="Rastogi, Nalin" sort="Rastogi, Nalin" uniqKey="Rastogi N" first="Nalin" last="Rastogi">Nalin Rastogi</name>
<name sortKey="Reischl, Udo" sort="Reischl, Udo" uniqKey="Reischl U" first="Udo" last="Reischl">Udo Reischl</name>
<name sortKey="Sola, Christophe" sort="Sola, Christophe" uniqKey="Sola C" first="Christophe" last="Sola">Christophe Sola</name>
<name sortKey="Wolf, Hans" sort="Wolf, Hans" uniqKey="Wolf H" first="Hans" last="Wolf">Hans Wolf</name>
<name sortKey="Zink, Albert R" sort="Zink, Albert R" uniqKey="Zink A" first="Albert R." last="Zink">Albert R. Zink</name>
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Characterization of Mycobacterium tuberculosis Complex DNAs from Egyptian Mummies by Spoligotyping

Characterization of Mycobacterium tuberculosis Complex DNAs from Egyptian Mummies by Spoligotyping

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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