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Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Identifieur interne : 003E50 ( PubMed/Corpus ); précédent : 003E49; suivant : 003E51

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Auteurs : Kathrin Gassei ; Hanna Valli ; Kyle E. Orwig

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2014.

RBID : pubmed:25173170

English descriptors

KwdEn :
- Animals, Cell Differentiation, Cell Lineage, Haplorhini, Humans, Immunohistochemistry (methods), Male, Mice, Seminiferous Tubules (cytology), Seminiferous Tubules (metabolism), Spermatogenesis, Spermatogonia (cytology), Spermatogonia (metabolism), Stem Cells (cytology), Stem Cells (metabolism).
MESH :
- cytology : Seminiferous Tubules, Spermatogonia, Stem Cells.
- metabolism : Seminiferous Tubules, Spermatogonia, Stem Cells.
- methods : Immunohistochemistry.
- Animals, Cell Differentiation, Cell Lineage, Haplorhini, Humans, Male, Mice, Spermatogenesis.

Abstract

Spermatogonial stem cells (SSCs) and undifferentiated progenitor spermatogonia in mammalian seminiferous tubules are organized in chains, connected by intracellular bridges. Clone size is generally related to stem cell potential, with shorter chains containing the majority of the stem cell population. Immunofluorescence detection of spermatogonia-specific proteins in whole-mount seminiferous tubule preparations is the only method that allows researchers to relate clone size with the molecular phenotype in spermatogenic lineage development. Here we describe in detail the method used to detect nuclear, cytoplasmic, and cell surface molecules in seminiferous tubules isolated from mouse, monkey, and human testes.

DOI: 10.1007/978-1-4939-1435-7_15
PubMed: 25173170

Links to Exploration step

pubmed:25173170

Le document en format XML

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<author><name sortKey="Gassei, Kathrin" sort="Gassei, Kathrin" uniqKey="Gassei K" first="Kathrin" last="Gassei">Kathrin Gassei</name>
<affiliation><nlm:affiliation>Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, 15213, USA.</nlm:affiliation>
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<author><name sortKey="Valli, Hanna" sort="Valli, Hanna" uniqKey="Valli H" first="Hanna" last="Valli">Hanna Valli</name>
</author>
<author><name sortKey="Orwig, Kyle E" sort="Orwig, Kyle E" uniqKey="Orwig K" first="Kyle E" last="Orwig">Kyle E. Orwig</name>
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<author><name sortKey="Valli, Hanna" sort="Valli, Hanna" uniqKey="Valli H" first="Hanna" last="Valli">Hanna Valli</name>
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<term>Immunohistochemistry (methods)</term>
<term>Male</term>
<term>Mice</term>
<term>Seminiferous Tubules (cytology)</term>
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<term>Spermatogonia</term>
<term>Stem Cells</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunohistochemistry</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Differentiation</term>
<term>Cell Lineage</term>
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<front><div type="abstract" xml:lang="en">Spermatogonial stem cells (SSCs) and undifferentiated progenitor spermatogonia in mammalian seminiferous tubules are organized in chains, connected by intracellular bridges. Clone size is generally related to stem cell potential, with shorter chains containing the majority of the stem cell population. Immunofluorescence detection of spermatogonia-specific proteins in whole-mount seminiferous tubule preparations is the only method that allows researchers to relate clone size with the molecular phenotype in spermatogenic lineage development. Here we describe in detail the method used to detect nuclear, cytoplasmic, and cell surface molecules in seminiferous tubules isolated from mouse, monkey, and human testes.</div>
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<Title>Methods in molecular biology (Clifton, N.J.)</Title>
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<MeshHeading><DescriptorName UI="D013091" MajorTopicYN="Y">Spermatogenesis</DescriptorName>
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<MeshHeading><DescriptorName UI="D013234" MajorTopicYN="N">Stem Cells</DescriptorName>
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Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki