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Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.

Identifieur interne : 000002 ( PubMed/Corpus ); précédent : 000001; suivant : 000003

Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.

Auteurs : Wesley N. Sivak ; James D. White ; Jacqueline M. Bliley ; Lee W. Tien ; Han Tsung Liao ; David L. Kaplan ; Kacey G. Marra

Source :

Journal of tissue engineering and regenerative medicine [ 1932-7005 ] ; 2017.

RBID : pubmed:25424415

Abstract

Nerve conduits are a proven strategy for guiding axon regrowth following injury. This study compares degradable silk-trehalose films containing chondroitinase ABC (ChABC) and/or glial cell line-derived neurotrophic factor (GDNF) loaded within a silk fibroin-based nerve conduit in a rat sciatic nerve defect model. Four groups of silk conduits were prepared, with the following silk-trehalose films inserted into the conduit: (a) empty; (b) 1 µg GDNF; (3) 2 U ChABC; and (4) 1 µg GDNF/2 U ChABC. Drug release studies demonstrated 20% recovery of GDNF and ChABC at 6 weeks and 24 h, respectively. Six conduits of each type were implanted into 15 mm sciatic nerve defects in Lewis rats; conduits were explanted for histological analysis at 6 weeks. Tissues stained with Schwann cell S-100 antibody demonstrated an increased density of cells in both GDNF- and ChABC-treated groups compared to empty control conduits (p < 0.05). Conduits loaded with GDNF and ChABC also demonstrated higher levels of neuron-specific PGP 9.5 protein when compared to controls (p < 0.05). In this study we demonstrated a method to enhance Schwann cell migration and proliferation and also foster axonal regeneration when repairing peripheral nerve gap defects. Silk fibroin-based nerve conduits possess favourable mechanical and degradative properties and are further enhanced when loaded with ChABC and GDNF. Copyright © 2014 John Wiley & Sons, Ltd.

DOI: 10.1002/term.1970
PubMed: 25424415

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<author><name sortKey="Sivak, Wesley N" sort="Sivak, Wesley N" uniqKey="Sivak W" first="Wesley N" last="Sivak">Wesley N. Sivak</name>
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<author><name sortKey="White, James D" sort="White, James D" uniqKey="White J" first="James D" last="White">James D. White</name>
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<author><name sortKey="Bliley, Jacqueline M" sort="Bliley, Jacqueline M" uniqKey="Bliley J" first="Jacqueline M" last="Bliley">Jacqueline M. Bliley</name>
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<author><name sortKey="Tien, Lee W" sort="Tien, Lee W" uniqKey="Tien L" first="Lee W" last="Tien">Lee W. Tien</name>
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<author><name sortKey="Liao, Han Tsung" sort="Liao, Han Tsung" uniqKey="Liao H" first="Han Tsung" last="Liao">Han Tsung Liao</name>
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<author><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L" last="Kaplan">David L. Kaplan</name>
<affiliation><nlm:affiliation>Department of Biomedical Engineering, Tufts University, Boston, MA, USA.</nlm:affiliation>
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<author><name sortKey="Marra, Kacey G" sort="Marra, Kacey G" uniqKey="Marra K" first="Kacey G" last="Marra">Kacey G. Marra</name>
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<front><div type="abstract" xml:lang="en">Nerve conduits are a proven strategy for guiding axon regrowth following injury. This study compares degradable silk-trehalose films containing chondroitinase ABC (ChABC) and/or glial cell line-derived neurotrophic factor (GDNF) loaded within a silk fibroin-based nerve conduit in a rat sciatic nerve defect model. Four groups of silk conduits were prepared, with the following silk-trehalose films inserted into the conduit: (a) empty; (b) 1 µg GDNF; (3) 2 U ChABC; and (4) 1 µg GDNF/2 U ChABC. Drug release studies demonstrated 20% recovery of GDNF and ChABC at 6 weeks and 24 h, respectively. Six conduits of each type were implanted into 15 mm sciatic nerve defects in Lewis rats; conduits were explanted for histological analysis at 6 weeks. Tissues stained with Schwann cell S-100 antibody demonstrated an increased density of cells in both GDNF- and ChABC-treated groups compared to empty control conduits (p < 0.05). Conduits loaded with GDNF and ChABC also demonstrated higher levels of neuron-specific PGP 9.5 protein when compared to controls (p < 0.05). In this study we demonstrated a method to enhance Schwann cell migration and proliferation and also foster axonal regeneration when repairing peripheral nerve gap defects. Silk fibroin-based nerve conduits possess favourable mechanical and degradative properties and are further enhanced when loaded with ChABC and GDNF. Copyright © 2014 John Wiley & Sons, Ltd.</div>
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<Month>11</Month>
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<DateRevised><Year>2017</Year>
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<Title>Journal of tissue engineering and regenerative medicine</Title>
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<ArticleTitle>Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.</ArticleTitle>
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<Abstract><AbstractText>Nerve conduits are a proven strategy for guiding axon regrowth following injury. This study compares degradable silk-trehalose films containing chondroitinase ABC (ChABC) and/or glial cell line-derived neurotrophic factor (GDNF) loaded within a silk fibroin-based nerve conduit in a rat sciatic nerve defect model. Four groups of silk conduits were prepared, with the following silk-trehalose films inserted into the conduit: (a) empty; (b) 1 µg GDNF; (3) 2 U ChABC; and (4) 1 µg GDNF/2 U ChABC. Drug release studies demonstrated 20% recovery of GDNF and ChABC at 6 weeks and 24 h, respectively. Six conduits of each type were implanted into 15 mm sciatic nerve defects in Lewis rats; conduits were explanted for histological analysis at 6 weeks. Tissues stained with Schwann cell S-100 antibody demonstrated an increased density of cells in both GDNF- and ChABC-treated groups compared to empty control conduits (p < 0.05). Conduits loaded with GDNF and ChABC also demonstrated higher levels of neuron-specific PGP 9.5 protein when compared to controls (p < 0.05). In this study we demonstrated a method to enhance Schwann cell migration and proliferation and also foster axonal regeneration when repairing peripheral nerve gap defects. Silk fibroin-based nerve conduits possess favourable mechanical and degradative properties and are further enhanced when loaded with ChABC and GDNF. Copyright © 2014 John Wiley & Sons, Ltd.</AbstractText>
<CopyrightInformation>Copyright © 2014 John Wiley & Sons, Ltd.</CopyrightInformation>
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<AffiliationInfo><Affiliation>Department of Plastic and Reconstructive Surgery, Craniofacial Research Centre, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan.</Affiliation>
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<AffiliationInfo><Affiliation>Department of Bioengineering, University of Pittsburgh, PA, USA.</Affiliation>
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<AffiliationInfo><Affiliation>McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA.</Affiliation>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">GDNF</Keyword>
<Keyword MajorTopicYN="N">chondroitinase ABC</Keyword>
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<Keyword MajorTopicYN="N">nerve conduit</Keyword>
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Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.

Delivery of chondroitinase ABC and glial cell line-derived neurotrophic factor from silk fibroin conduits enhances peripheral nerve regeneration.

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Abstract

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