Hemoglobin α/eNOS coupling at myoendothelial junctions is required for nitric oxide scavenging during vasoconstriction.
Identifieur interne : 002A45 ( PubMed/Checkpoint ); précédent : 002A44; suivant : 002A46Hemoglobin α/eNOS coupling at myoendothelial junctions is required for nitric oxide scavenging during vasoconstriction.
Auteurs : Adam C. Straub [États-Unis] ; Joshua T. Butcher [États-Unis] ; Marie Billaud [États-Unis] ; Stephanie M. Mutchler [États-Unis] ; Mykhaylo V. Artamonov [États-Unis] ; Anh T. Nguyen [États-Unis] ; Tyler Johnson [États-Unis] ; Angela K. Best [États-Unis] ; Megan P. Miller [États-Unis] ; Lisa A. Palmer [États-Unis] ; Linda Columbus [États-Unis] ; Avril V. Somlyo [États-Unis] ; Thu H. Le [États-Unis] ; Brant E. Isakson [États-Unis]Source :
- Arteriosclerosis, thrombosis, and vascular biology [ 1524-4636 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Cellules endothéliales (métabolisme), Données de séquences moléculaires, Globines alpha (), Globines alpha (génétique), Globines alpha (métabolisme), Humains, Jonctions intercellulaires (physiologie), Modèles moléculaires, Monoxyde d'azote (métabolisme), Motifs et domaines d'intéraction protéique, Mutagenèse dirigée, Myocytes du muscle lisse (métabolisme), Mâle, Nitric oxide synthase type III (déficit), Nitric oxide synthase type III (génétique), Nitric oxide synthase type III (métabolisme), Pression sanguine (physiologie), Protéines recombinantes (), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Résistance vasculaire (physiologie), Simulation numérique, Souris, Souris de lignée C57BL, Souris knockout, Séquence conservée, Séquence d'acides aminés, Transduction du signal, Vasoconstriction (physiologie).
- MESH :
- déficit : Nitric oxide synthase type III.
- génétique : Globines alpha, Nitric oxide synthase type III, Protéines recombinantes.
- métabolisme : Cellules endothéliales, Globines alpha, Monoxyde d'azote, Myocytes du muscle lisse, Nitric oxide synthase type III, Protéines recombinantes.
- physiologie : Jonctions intercellulaires, Pression sanguine, Résistance vasculaire, Vasoconstriction.
- Animaux, Cellules cultivées, Données de séquences moléculaires, Globines alpha, Humains, Modèles moléculaires, Motifs et domaines d'intéraction protéique, Mutagenèse dirigée, Mâle, Protéines recombinantes, Simulation numérique, Souris, Souris de lignée C57BL, Souris knockout, Séquence conservée, Séquence d'acides aminés, Transduction du signal.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Blood Pressure (physiology), Cells, Cultured, Computer Simulation, Conserved Sequence, Endothelial Cells (metabolism), Humans, Intercellular Junctions (physiology), Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Myocytes, Smooth Muscle (metabolism), Nitric Oxide (metabolism), Nitric Oxide Synthase Type III (deficiency), Nitric Oxide Synthase Type III (genetics), Nitric Oxide Synthase Type III (metabolism), Protein Interaction Domains and Motifs, Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Signal Transduction, Vascular Resistance (physiology), Vasoconstriction (physiology), alpha-Globins (chemistry), alpha-Globins (genetics), alpha-Globins (metabolism).
- MESH :
- chemical , chemistry : Recombinant Proteins, alpha-Globins.
- chemical , deficiency : Nitric Oxide Synthase Type III.
- chemical , genetics : Nitric Oxide Synthase Type III, Recombinant Proteins, alpha-Globins.
- chemical , metabolism : Nitric Oxide, Nitric Oxide Synthase Type III, Recombinant Proteins, alpha-Globins.
- metabolism : Endothelial Cells, Myocytes, Smooth Muscle.
- physiology : Blood Pressure, Intercellular Junctions, Vascular Resistance, Vasoconstriction.
- Amino Acid Sequence, Animals, Cells, Cultured, Computer Simulation, Conserved Sequence, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Interaction Domains and Motifs, Signal Transduction.
Abstract
Hemoglobin α (Hb α) and endothelial nitric oxide synthase (eNOS) form a macromolecular complex at myoendothelial junctions; the functional role of this interaction remains undefined. To test if coupling of eNOS and Hb α regulates nitric oxide signaling, vascular reactivity, and blood pressure using a mimetic peptide of Hb α to disrupt this interaction.
DOI: 10.1161/ATVBAHA.114.303974
PubMed: 25278292
Affiliations:
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pubmed:25278292Le document en format XML
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Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Butcher, Joshua T" sort="Butcher, Joshua T" uniqKey="Butcher J" first="Joshua T" last="Butcher">Joshua T. Butcher</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Billaud, Marie" sort="Billaud, Marie" uniqKey="Billaud M" first="Marie" last="Billaud">Marie Billaud</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Mutchler, Stephanie M" sort="Mutchler, Stephanie M" uniqKey="Mutchler S" first="Stephanie M" last="Mutchler">Stephanie M. Mutchler</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Artamonov, Mykhaylo V" sort="Artamonov, Mykhaylo V" uniqKey="Artamonov M" first="Mykhaylo V" last="Artamonov">Mykhaylo V. Artamonov</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Nguyen, Anh T" sort="Nguyen, Anh T" uniqKey="Nguyen A" first="Anh T" last="Nguyen">Anh T. Nguyen</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Johnson, Tyler" sort="Johnson, Tyler" uniqKey="Johnson T" first="Tyler" last="Johnson">Tyler Johnson</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Best, Angela K" sort="Best, Angela K" uniqKey="Best A" first="Angela K" last="Best">Angela K. Best</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Miller, Megan P" sort="Miller, Megan P" uniqKey="Miller M" first="Megan P" last="Miller">Megan P. Miller</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Palmer, Lisa A" sort="Palmer, Lisa A" uniqKey="Palmer L" first="Lisa A" last="Palmer">Lisa A. Palmer</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Columbus, Linda" sort="Columbus, Linda" uniqKey="Columbus L" first="Linda" last="Columbus">Linda Columbus</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Somlyo, Avril V" sort="Somlyo, Avril V" uniqKey="Somlyo A" first="Avril V" last="Somlyo">Avril V. Somlyo</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Le, Thu H" sort="Le, Thu H" uniqKey="Le T" first="Thu H" last="Le">Thu H. Le</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Isakson, Brant E" sort="Isakson, Brant E" uniqKey="Isakson B" first="Brant E" last="Isakson">Brant E. Isakson</name><affiliation wicri:level="1"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville. brant@virginia.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25278292</idno><idno type="pmid">25278292</idno><idno type="doi">10.1161/ATVBAHA.114.303974</idno><idno type="wicri:Area/PubMed/Corpus">000F04</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000F04</idno><idno type="wicri:Area/PubMed/Curation">000F01</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000F01</idno><idno type="wicri:Area/PubMed/Checkpoint">000F01</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000F01</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Hemoglobin α/eNOS coupling at myoendothelial junctions is required for nitric oxide scavenging during vasoconstriction.</title><author><name sortKey="Straub, Adam C" sort="Straub, Adam C" uniqKey="Straub A" first="Adam C" last="Straub">Adam C. Straub</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Butcher, Joshua T" sort="Butcher, Joshua T" uniqKey="Butcher J" first="Joshua T" last="Butcher">Joshua T. Butcher</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Billaud, Marie" sort="Billaud, Marie" uniqKey="Billaud M" first="Marie" last="Billaud">Marie Billaud</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Mutchler, Stephanie M" sort="Mutchler, Stephanie M" uniqKey="Mutchler S" first="Stephanie M" last="Mutchler">Stephanie M. Mutchler</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Artamonov, Mykhaylo V" sort="Artamonov, Mykhaylo V" uniqKey="Artamonov M" first="Mykhaylo V" last="Artamonov">Mykhaylo V. Artamonov</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Nguyen, Anh T" sort="Nguyen, Anh T" uniqKey="Nguyen A" first="Anh T" last="Nguyen">Anh T. Nguyen</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Johnson, Tyler" sort="Johnson, Tyler" uniqKey="Johnson T" first="Tyler" last="Johnson">Tyler Johnson</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Best, Angela K" sort="Best, Angela K" uniqKey="Best A" first="Angela K" last="Best">Angela K. Best</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Miller, Megan P" sort="Miller, Megan P" uniqKey="Miller M" first="Megan P" last="Miller">Megan P. Miller</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Palmer, Lisa A" sort="Palmer, Lisa A" uniqKey="Palmer L" first="Lisa A" last="Palmer">Lisa A. Palmer</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Columbus, Linda" sort="Columbus, Linda" uniqKey="Columbus L" first="Linda" last="Columbus">Linda Columbus</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Somlyo, Avril V" sort="Somlyo, Avril V" uniqKey="Somlyo A" first="Avril V" last="Somlyo">Avril V. Somlyo</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Le, Thu H" sort="Le, Thu H" uniqKey="Le T" first="Thu H" last="Le">Thu H. Le</name><affiliation wicri:level="4"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</nlm:affiliation><orgName type="university">Université de Pittsburgh</orgName><country>États-Unis</country><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Isakson, Brant E" sort="Isakson, Brant E" uniqKey="Isakson B" first="Brant E" last="Isakson">Brant E. Isakson</name><affiliation wicri:level="1"><nlm:affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville. brant@virginia.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><series><title level="j">Arteriosclerosis, thrombosis, and vascular biology</title><idno type="eISSN">1524-4636</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blood Pressure (physiology)</term><term>Cells, Cultured</term><term>Computer Simulation</term><term>Conserved Sequence</term><term>Endothelial Cells (metabolism)</term><term>Humans</term><term>Intercellular Junctions (physiology)</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Myocytes, Smooth Muscle (metabolism)</term><term>Nitric Oxide (metabolism)</term><term>Nitric Oxide Synthase Type III (deficiency)</term><term>Nitric Oxide Synthase Type III (genetics)</term><term>Nitric Oxide Synthase Type III (metabolism)</term><term>Protein Interaction Domains and Motifs</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Signal Transduction</term><term>Vascular Resistance (physiology)</term><term>Vasoconstriction (physiology)</term><term>alpha-Globins (chemistry)</term><term>alpha-Globins (genetics)</term><term>alpha-Globins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Cellules endothéliales (métabolisme)</term><term>Données de séquences moléculaires</term><term>Globines alpha ()</term><term>Globines alpha (génétique)</term><term>Globines alpha (métabolisme)</term><term>Humains</term><term>Jonctions intercellulaires (physiologie)</term><term>Modèles moléculaires</term><term>Monoxyde d'azote (métabolisme)</term><term>Motifs et domaines d'intéraction protéique</term><term>Mutagenèse dirigée</term><term>Myocytes du muscle lisse (métabolisme)</term><term>Mâle</term><term>Nitric oxide synthase type III (déficit)</term><term>Nitric oxide synthase type III (génétique)</term><term>Nitric oxide synthase type III (métabolisme)</term><term>Pression sanguine (physiologie)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Résistance vasculaire (physiologie)</term><term>Simulation numérique</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term><term>Transduction du signal</term><term>Vasoconstriction (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Recombinant Proteins</term><term>alpha-Globins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Nitric Oxide Synthase Type III</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nitric Oxide Synthase Type III</term><term>Recombinant Proteins</term><term>alpha-Globins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitric Oxide</term><term>Nitric Oxide Synthase Type III</term><term>Recombinant Proteins</term><term>alpha-Globins</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Nitric oxide synthase type III</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Globines alpha</term><term>Nitric oxide synthase type III</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endothelial Cells</term><term>Myocytes, Smooth Muscle</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules endothéliales</term><term>Globines alpha</term><term>Monoxyde d'azote</term><term>Myocytes du muscle lisse</term><term>Nitric oxide synthase type III</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Jonctions intercellulaires</term><term>Pression sanguine</term><term>Résistance vasculaire</term><term>Vasoconstriction</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Blood Pressure</term><term>Intercellular Junctions</term><term>Vascular Resistance</term><term>Vasoconstriction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cells, Cultured</term><term>Computer Simulation</term><term>Conserved Sequence</term><term>Humans</term><term>Male</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Protein Interaction Domains and Motifs</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Globines alpha</term><term>Humains</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Mutagenèse dirigée</term><term>Mâle</term><term>Protéines recombinantes</term><term>Simulation numérique</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hemoglobin α (Hb α) and endothelial nitric oxide synthase (eNOS) form a macromolecular complex at myoendothelial junctions; the functional role of this interaction remains undefined. To test if coupling of eNOS and Hb α regulates nitric oxide signaling, vascular reactivity, and blood pressure using a mimetic peptide of Hb α to disrupt this interaction.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25278292</PMID><DateCreated><Year>2014</Year><Month>11</Month><Day>21</Day></DateCreated><DateCompleted><Year>2015</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1524-4636</ISSN><JournalIssue CitedMedium="Internet"><Volume>34</Volume><Issue>12</Issue><PubDate><Year>2014</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Arteriosclerosis, thrombosis, and vascular biology</Title><ISOAbbreviation>Arterioscler. Thromb. Vasc. Biol.</ISOAbbreviation></Journal><ArticleTitle>Hemoglobin α/eNOS coupling at myoendothelial junctions is required for nitric oxide scavenging during vasoconstriction.</ArticleTitle><Pagination><MedlinePgn>2594-600</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1161/ATVBAHA.114.303974</ELocationID><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Hemoglobin α (Hb α) and endothelial nitric oxide synthase (eNOS) form a macromolecular complex at myoendothelial junctions; the functional role of this interaction remains undefined. To test if coupling of eNOS and Hb α regulates nitric oxide signaling, vascular reactivity, and blood pressure using a mimetic peptide of Hb α to disrupt this interaction.</AbstractText><AbstractText Label="APPROACH AND RESULTS" NlmCategory="RESULTS">In silico modeling of Hb α and eNOS identified a conserved sequence of interaction. By mutating portions of Hb α, we identified a specific sequence that binds eNOS. A mimetic peptide of the Hb α sequence (Hb α X) was generated to disrupt this complex. Using in vitro binding assays with purified Hb α and eNOS and ex vivo proximity ligation assays on resistance arteries, we have demonstrated that Hb α X significantly decreased interaction between eNOS and Hb α. Fluorescein isothiocyanate labeling of Hb α X revealed localization to holes in the internal elastic lamina (ie, myoendothelial junctions). To test the functional effects of Hb α X, we measured cyclic guanosine monophosphate and vascular reactivity. Our results reveal augmented cyclic guanosine monophosphate production and altered vasoconstriction with Hb α X. To test the in vivo effects of these peptides on blood pressure, normotensive and hypertensive mice were injected with Hb α X, which caused a significant decrease in blood pressure; injection of Hb α X into eNOS(-/-) mice had no effect.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These results identify a novel sequence on Hb α that is important for Hb α/eNOS complex formation and is critical for nitric oxide signaling at myoendothelial junctions.</AbstractText><CopyrightInformation>© 2014 American Heart Association, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Straub</LastName><ForeName>Adam C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Butcher</LastName><ForeName>Joshua T</ForeName><Initials>JT</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Billaud</LastName><ForeName>Marie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mutchler</LastName><ForeName>Stephanie M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Artamonov</LastName><ForeName>Mykhaylo V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Anh T</ForeName><Initials>AT</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>Tyler</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Best</LastName><ForeName>Angela K</ForeName><Initials>AK</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miller</LastName><ForeName>Megan P</ForeName><Initials>MP</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Palmer</LastName><ForeName>Lisa A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Columbus</LastName><ForeName>Linda</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Somlyo</LastName><ForeName>Avril V</ForeName><Initials>AV</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Le</LastName><ForeName>Thu H</ForeName><Initials>TH</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Isakson</LastName><ForeName>Brant E</ForeName><Initials>BE</Initials><AffiliationInfo><Affiliation>From the Department of Pharmacology and Chemical Biology (A.C.S.) and Heart, Lung, Blood and Vascular Medicine Institute (A.C.S., A.T.N., M.P.M.), University of Pittsburgh, PA; Robert M. Berne Cardiovascular Research Center (J.T.B., M.B., S.M.M., T.J., A.K.B., B.E.I.), Department of Molecular Physiology and Biophysics (M.B., M.V.A., A.V.S., B.E.I.), Deparment of Pediatrics (L.A.P.), Department of Chemistry (L.C.), and Department of Medicine (T.H.L.), University of Virginia, Charlottesville. brant@virginia.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 GM087828</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>DK06129611</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>DK088905</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 HL107963</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>GM087828</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>HL112904</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R00 HL112904</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>K99 HL112904</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>GM086457</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>HL088554</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>HL107963</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 DK088905</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P01 HL101871</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 HL088554</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32 DK061296</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32 HL007284</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 GM086457</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>10</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Arterioscler Thromb Vasc Biol</MedlineTA><NlmUniqueID>9505803</NlmUniqueID><ISSNLinking>1079-5642</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D055542">alpha-Globins</NameOfSubstance></Chemical><Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber><NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.13.39</RegistryNumber><NameOfSubstance UI="D052250">Nitric Oxide Synthase Type III</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.13.39</RegistryNumber><NameOfSubstance UI="C496315">Nos3 protein, 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MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001794" MajorTopicYN="N">Blood Pressure</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003198" MajorTopicYN="N">Computer Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042783" MajorTopicYN="N">Endothelial Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007365" MajorTopicYN="N">Intercellular Junctions</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018345" MajorTopicYN="N">Mice, Knockout</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032389" MajorTopicYN="N">Myocytes, Smooth Muscle</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052250" MajorTopicYN="N">Nitric Oxide Synthase Type III</DescriptorName><QualifierName UI="Q000172" MajorTopicYN="N">deficiency</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054730" MajorTopicYN="N">Protein Interaction Domains and Motifs</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014655" MajorTopicYN="N">Vascular Resistance</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014661" MajorTopicYN="N">Vasoconstriction</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055542" MajorTopicYN="N">alpha-Globins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">NIHMS630200</OtherID><OtherID Source="NLM">PMC4239174</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">endothelial cells</Keyword><Keyword MajorTopicYN="N">endothelial nitric oxide synthase</Keyword><Keyword MajorTopicYN="N">hemoglobin α</Keyword><Keyword MajorTopicYN="N">nitric oxide</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>4</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25278292</ArticleId><ArticleId IdType="pii">ATVBAHA.114.303974</ArticleId><ArticleId IdType="doi">10.1161/ATVBAHA.114.303974</ArticleId><ArticleId IdType="pmc">PMC4239174</ArticleId><ArticleId IdType="mid">NIHMS630200</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Straub, Adam C" sort="Straub, Adam C" uniqKey="Straub A" first="Adam C" last="Straub">Adam C. Straub</name></region><name sortKey="Artamonov, Mykhaylo V" sort="Artamonov, Mykhaylo V" uniqKey="Artamonov M" first="Mykhaylo V" last="Artamonov">Mykhaylo V. Artamonov</name><name sortKey="Best, Angela K" sort="Best, Angela K" uniqKey="Best A" first="Angela K" last="Best">Angela K. Best</name><name sortKey="Billaud, Marie" sort="Billaud, Marie" uniqKey="Billaud M" first="Marie" last="Billaud">Marie Billaud</name><name sortKey="Butcher, Joshua T" sort="Butcher, Joshua T" uniqKey="Butcher J" first="Joshua T" last="Butcher">Joshua T. Butcher</name><name sortKey="Columbus, Linda" sort="Columbus, Linda" uniqKey="Columbus L" first="Linda" last="Columbus">Linda Columbus</name><name sortKey="Isakson, Brant E" sort="Isakson, Brant E" uniqKey="Isakson B" first="Brant E" last="Isakson">Brant E. Isakson</name><name sortKey="Johnson, Tyler" sort="Johnson, Tyler" uniqKey="Johnson T" first="Tyler" last="Johnson">Tyler Johnson</name><name sortKey="Le, Thu H" sort="Le, Thu H" uniqKey="Le T" first="Thu H" last="Le">Thu H. Le</name><name sortKey="Miller, Megan P" sort="Miller, Megan P" uniqKey="Miller M" first="Megan P" last="Miller">Megan P. Miller</name><name sortKey="Mutchler, Stephanie M" sort="Mutchler, Stephanie M" uniqKey="Mutchler S" first="Stephanie M" last="Mutchler">Stephanie M. Mutchler</name><name sortKey="Nguyen, Anh T" sort="Nguyen, Anh T" uniqKey="Nguyen A" first="Anh T" last="Nguyen">Anh T. Nguyen</name><name sortKey="Palmer, Lisa A" sort="Palmer, Lisa A" uniqKey="Palmer L" first="Lisa A" last="Palmer">Lisa A. Palmer</name><name sortKey="Somlyo, Avril V" sort="Somlyo, Avril V" uniqKey="Somlyo A" first="Avril V" last="Somlyo">Avril V. Somlyo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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