Modeling human gastrointestinal inflammatory diseases using microphysiological culture systems
Identifieur interne : 000F84 ( Pmc/Curation ); précédent : 000F83; suivant : 000F85Modeling human gastrointestinal inflammatory diseases using microphysiological culture systems
Auteurs : Kira G. Hartman [États-Unis] ; James D. Bortner [États-Unis] ; Gary W. Falk [États-Unis] ; Gregory G. Ginsberg [États-Unis] ; Nirag Jhala [États-Unis] ; Jian Yu [États-Unis] ; Martín G. Martín [États-Unis] ; Anil K. Rustgi [États-Unis] ; John P. Lynch [États-Unis]Source :
- Experimental biology and medicine (Maywood, N.J.) [ 1535-3702 ] ; 2014.
Abstract
Gastrointestinal (GI) illnesses are a significant health burden for the US population, with 40 million office visits each year for gastrointestinal complaints and nearly 250,000 deaths. Acute and chronic inflammation are a common element of many GI diseases. Inflammatory processes may be initiated by a chemical injury (acid reflux in the esophagus), an infectious agent (
Url:
DOI: 10.1177/1535370214529388
PubMed: 24781339
PubMed Central: 4156523
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<front><div type="abstract" xml:lang="en"><p id="P1">Gastrointestinal (GI) illnesses are a significant health burden for the US population, with 40 million office visits each year for gastrointestinal complaints and nearly 250,000 deaths. Acute and chronic inflammation are a common element of many GI diseases. Inflammatory processes may be initiated by a chemical injury (acid reflux in the esophagus), an infectious agent (<italic>Helicobacter pylori</italic>
infection in the stomach), autoimmune processes (graft versus host disease after bone marrow transplantation), or idiopathic (as in the case of inflammatory bowel diseases). Inflammation in these settings can contribute to acute complaints (pain, bleeding, obstruction, diarrhea) as well as chronic sequelae including strictures and cancer. Research into the pathophysiology of these conditions has been limited by the availability of primary human tissues or appropriate animal models that attempt to physiologically model the human disease. With the many recent advances in tissue engineering and primary human cell culture systems, it is conceivable that these approaches can be adapted to develop novel human <italic>ex vivo</italic>
systems that incorporate many human cell types to recapitulate <italic>in vivo</italic>
growth and differentiation in inflammatory microphysiological environments. Such an advance in technology would improve our understanding of human disease progression and enhance our ability to test for disease prevention strategies and novel therapeutics. We will review current models for the inflammatory and immunological aspects of Barrett’s esophagus, acute graft versus host disease, and inflammatory bowel disease and explore recent advances in culture methodologies that make these novel microphysiological research systems possible.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
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<front><journal-meta><journal-id journal-id-type="nlm-journal-id">100973463</journal-id>
<journal-id journal-id-type="pubmed-jr-id">21987</journal-id>
<journal-id journal-id-type="nlm-ta">Exp Biol Med (Maywood)</journal-id>
<journal-id journal-id-type="iso-abbrev">Exp. Biol. Med. (Maywood)</journal-id>
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<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
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<title-group><article-title>Modeling human gastrointestinal inflammatory diseases using microphysiological culture systems</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Hartman</surname>
<given-names>Kira G.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref rid="FN2" ref-type="author-notes">*</xref>
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<contrib contrib-type="author"><name><surname>Bortner</surname>
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<contrib contrib-type="author"><name><surname>Falk</surname>
<given-names>Gary W.</given-names>
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<xref ref-type="aff" rid="A1">1</xref>
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<contrib contrib-type="author"><name><surname>Ginsberg</surname>
<given-names>Gregory G.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jhala</surname>
<given-names>Nirag</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
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<contrib contrib-type="author"><name><surname>Yu</surname>
<given-names>Jian</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Martín</surname>
<given-names>Martín G.</given-names>
</name>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rustgi</surname>
<given-names>Anil K.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lynch</surname>
<given-names>John P.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
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<aff id="A1"><label>1</label>
Division of Gastroenterology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104 USA</aff>
<aff id="A2"><label>2</label>
Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104 USA</aff>
<aff id="A3"><label>3</label>
Departments of Pathology, and Radiation Oncology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, 15213 USA</aff>
<aff id="A4"><label>4</label>
Department of Pediatrics, Division of Gastroenterology and Nutrition, Mattel Children’s Hospital and the David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, 90095 USA</aff>
<author-notes><corresp id="FN1">Corresponding Author: John P. Lynch, M.D. Ph.D., Associate Professor of Medicine, Division of Gastroenterology, University of Pennsylvania Perelman School of Medicine, 421 Curie Blvd. /912 BRB, Philadelphia, PA 19104, <email>lynchj@mail.med.upenn.edu</email>
, Tel (215) 898-0155, Fax (215) 573-2024</corresp>
<fn id="FN2" fn-type="equal"><label>*</label>
<p>Denotes equal authorship contributions</p>
</fn>
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<pub-date pub-type="nihms-submitted"><day>13</day>
<month>6</month>
<year>2014</year>
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<month>4</month>
<year>2014</year>
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<pub-date pub-type="ppub"><month>9</month>
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<pub-date pub-type="pmc-release"><day>01</day>
<month>9</month>
<year>2015</year>
</pub-date>
<volume>239</volume>
<issue>9</issue>
<fpage>1108</fpage>
<lpage>1123</lpage>
<pmc-comment>elocation-id from pubmed: 10.1177/1535370214529388</pmc-comment>
<abstract><p id="P1">Gastrointestinal (GI) illnesses are a significant health burden for the US population, with 40 million office visits each year for gastrointestinal complaints and nearly 250,000 deaths. Acute and chronic inflammation are a common element of many GI diseases. Inflammatory processes may be initiated by a chemical injury (acid reflux in the esophagus), an infectious agent (<italic>Helicobacter pylori</italic>
infection in the stomach), autoimmune processes (graft versus host disease after bone marrow transplantation), or idiopathic (as in the case of inflammatory bowel diseases). Inflammation in these settings can contribute to acute complaints (pain, bleeding, obstruction, diarrhea) as well as chronic sequelae including strictures and cancer. Research into the pathophysiology of these conditions has been limited by the availability of primary human tissues or appropriate animal models that attempt to physiologically model the human disease. With the many recent advances in tissue engineering and primary human cell culture systems, it is conceivable that these approaches can be adapted to develop novel human <italic>ex vivo</italic>
systems that incorporate many human cell types to recapitulate <italic>in vivo</italic>
growth and differentiation in inflammatory microphysiological environments. Such an advance in technology would improve our understanding of human disease progression and enhance our ability to test for disease prevention strategies and novel therapeutics. We will review current models for the inflammatory and immunological aspects of Barrett’s esophagus, acute graft versus host disease, and inflammatory bowel disease and explore recent advances in culture methodologies that make these novel microphysiological research systems possible.</p>
</abstract>
<kwd-group><kwd>inflammation</kwd>
<kwd>oxidative stress</kwd>
<kwd>autophagy</kwd>
<kwd>gastrointestinal (GI) disease</kwd>
<kwd>gastroesophageal reflux disease (GERD)</kwd>
<kwd>Barrett’s esophagus (BE)</kwd>
<kwd>esophageal adenocarcinoma (EAC)</kwd>
<kwd>graft-versus-host disease (GvHD)</kwd>
<kwd>inflammatory bowel disease (IBD)</kwd>
<kwd>human 3D organotypic model systems (OTC)</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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