Insights into copper coordination in the EcoRI–DNA complex by ESR spectroscopy
Identifieur interne : 000F33 ( Pmc/Checkpoint ); précédent : 000F32; suivant : 000F34Insights into copper coordination in the EcoRI–DNA complex by ESR spectroscopy
Auteurs : Ming Ji [États-Unis] ; Likun Tan [États-Unis] ; Linda Jen-Jacobson [États-Unis] ; Sunil Saxena [États-Unis]Source :
- Molecular physics [ 0026-8976 ] ; 2014.
Abstract
The EcoRI restriction endonuclease requires one divalent metal ion in each of two symmetrical and identical catalytic sites to catalyse double-strand DNA cleavage. Recently, we showed that Cu2+ binds outside the catalytic sites to a pair of new sites at H114 in each sub-unit, and inhibits Mg2+ -catalysed DNA cleavage. In order to provide more detailed structural information on this new metal ion binding site, we performed W-band (~94 GHz) and X-band (~9.5 GHz) electron spin resonance spectroscopic measurements on the EcoRI–DNA–(Cu2+ )2 complex. Cu2+ binding results in two distinct components with different
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DOI: 10.1080/00268976.2014.934313
PubMed: 25750461
PubMed Central: 4350447
Affiliations:
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<front><div type="abstract" xml:lang="en"><p id="P1">The EcoRI restriction endonuclease requires one divalent metal ion in each of two symmetrical and identical catalytic sites to catalyse double-strand DNA cleavage. Recently, we showed that Cu<sup>2+</sup>
binds outside the catalytic sites to a pair of new sites at H114 in each sub-unit, and inhibits Mg<sup>2+</sup>
-catalysed DNA cleavage. In order to provide more detailed structural information on this new metal ion binding site, we performed W-band (~94 GHz) and X-band (~9.5 GHz) electron spin resonance spectroscopic measurements on the EcoRI–DNA–(Cu<sup>2+</sup>
)<sub>2</sub>
complex. Cu<sup>2+</sup>
binding results in two distinct components with different <italic>g</italic>
<sub>zz</sub>
and <italic>A</italic>
<sub>zz</sub>
values. X-band electron spin echo envelope modulation results indicate that both components arise from a Cu<sup>2+</sup>
coordinated to histidine. This observation is further confirmed by the hyperfine sub-level correlation results. W-band electron nuclear double resonance spectra provide evidence for equatorial coordination of water molecules to the Cu<sup>2+</sup>
ions.</p>
</div>
</front>
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<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9876329</journal-id>
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<journal-id journal-id-type="nlm-ta">Mol Phys</journal-id>
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<title-group><article-title>Insights into copper coordination in the EcoRI–DNA complex by ESR spectroscopy</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Ji</surname>
<given-names>Ming</given-names>
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<contrib contrib-type="author"><name><surname>Tan</surname>
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<contrib contrib-type="author"><name><surname>Jen-Jacobson</surname>
<given-names>Linda</given-names>
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<contrib contrib-type="author"><name><surname>Saxena</surname>
<given-names>Sunil</given-names>
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<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="corresp" rid="CR1">*</xref>
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<aff id="A1"><label>a</label>
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA</aff>
<aff id="A2"><label>b</label>
Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA, USA</aff>
<aff id="A3"><label>c</label>
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA</aff>
<author-notes><corresp id="CR1"><label>*</label>
Corresponding author. <email>sksaxena@pitt.edu</email>
</corresp>
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<pub-date pub-type="nihms-submitted"><day>26</day>
<month>2</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub"><day>10</day>
<month>7</month>
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<pub-date pub-type="ppub"><day>1</day>
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<pub-date pub-type="pmc-release"><day>05</day>
<month>3</month>
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</pub-date>
<volume>112</volume>
<issue>24</issue>
<fpage>3173</fpage>
<lpage>3182</lpage>
<pmc-comment>elocation-id from pubmed: 10.1080/00268976.2014.934313</pmc-comment>
<permissions><copyright-statement>© 2014 Taylor & Francis</copyright-statement>
<copyright-year>2014</copyright-year>
<license><license-p>This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at <ext-link ext-link-type="uri" xlink:href="http://www.tandfonline.com/page/terms-and-conditions">http://www.tandfonline.com/page/terms-and-conditions</ext-link>
</license-p>
</license>
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<abstract><p id="P1">The EcoRI restriction endonuclease requires one divalent metal ion in each of two symmetrical and identical catalytic sites to catalyse double-strand DNA cleavage. Recently, we showed that Cu<sup>2+</sup>
binds outside the catalytic sites to a pair of new sites at H114 in each sub-unit, and inhibits Mg<sup>2+</sup>
-catalysed DNA cleavage. In order to provide more detailed structural information on this new metal ion binding site, we performed W-band (~94 GHz) and X-band (~9.5 GHz) electron spin resonance spectroscopic measurements on the EcoRI–DNA–(Cu<sup>2+</sup>
)<sub>2</sub>
complex. Cu<sup>2+</sup>
binding results in two distinct components with different <italic>g</italic>
<sub>zz</sub>
and <italic>A</italic>
<sub>zz</sub>
values. X-band electron spin echo envelope modulation results indicate that both components arise from a Cu<sup>2+</sup>
coordinated to histidine. This observation is further confirmed by the hyperfine sub-level correlation results. W-band electron nuclear double resonance spectra provide evidence for equatorial coordination of water molecules to the Cu<sup>2+</sup>
ions.</p>
</abstract>
<kwd-group><kwd>W-band ENDOR</kwd>
<kwd>ESEEM</kwd>
<kwd>metal ion coordination</kwd>
<kwd>Cu<sup>2+</sup>
inhibition</kwd>
<kwd>restriction endonuclease</kwd>
</kwd-group>
</article-meta>
</front>
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<li>Pennsylvanie</li>
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</settlement>
<orgName><li>Université de Pittsburgh</li>
</orgName>
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</region>
<name sortKey="Jen Jacobson, Linda" sort="Jen Jacobson, Linda" uniqKey="Jen Jacobson L" first="Linda" last="Jen-Jacobson">Linda Jen-Jacobson</name>
<name sortKey="Saxena, Sunil" sort="Saxena, Sunil" uniqKey="Saxena S" first="Sunil" last="Saxena">Sunil Saxena</name>
<name sortKey="Tan, Likun" sort="Tan, Likun" uniqKey="Tan L" first="Likun" last="Tan">Likun Tan</name>
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