PittsburghV1, PascalFrancis, Curation, bibRecord, 003C29

Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer

Identifieur interne : 003C29 ( PascalFrancis/Curation ); précédent : 003C28; suivant : 003C30

Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer

Auteurs : Julia A. Beaver [États-Unis] ; Danijela Jelovac [États-Unis] ; Sasidharan Balukrishna [Inde] ; Rory L. Cochran [États-Unis] ; Sarah Croessmann [États-Unis] ; Daniel J. Zabransky [États-Unis] ; HONG YUEN WONG [États-Unis] ; Patricia Valda Toro [États-Unis] ; Justin Cidado [États-Unis] ; Brian G. Blair [États-Unis] ; David Chu [États-Unis] ; Timothy Burns [États-Unis] ; Michaela J. Higgins [États-Unis] ; Vered Stearns [États-Unis] ; Lisa Jacobs [États-Unis] ; Mehran Habibi [États-Unis] ; Julie Lange [États-Unis] ; Paula J. Hurley [États-Unis] ; Josh Lauring [États-Unis] ; Dustin A. Vandenberg [États-Unis] ; Jill Kessler [États-Unis] ; Stacie Jeter [États-Unis] ; Michael L. Samuels [États-Unis] ; Dianna Maar [États-Unis] ; Leslie Cope ; Ashley Cimino-Mathews ; Pedram Argani ; Antonio C. Wolff ; BEN HO PARK

Source :

Clinical cancer research : (Print) [ 1078-0432 ] ; 2014.

RBID : Pascal:14-0157621

Descripteurs français

Pascal (Inist)
- Détection, Diagnostic, Tumeur maligne, Cancer du sein, DNA, Liquide biologique, Plasma sanguin, Homme, Stade précoce.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Biological fluid, Blood plasma, Breast cancer, DNA, Detection, Diagnosis, Early stage, Human, Malignant tumor.

Abstract

Purpose: Detecting circulating plasma tumor DNA (ptDNA) in patients with early-stage cancer has the potential to change how oncologists recommend systemic therapies for solid tumors after surgery. Droplet digital polymerase chain reaction (ddPCR) is a novel sensitive and specific platform for mutation detection. Experimental Design: In this prospective study, primary breast tumors and matched pre- and postsurgery blood samples were collected from patients with early-stage breast cancer (n = 29). Tumors (n = 30) were analyzed by Sanger sequencing for common PIK3CA mutations, and DNA from these tumors and matched plasma were then analyzed for PIK3CA mutations using ddPCR. Results: Sequencing of tumors identified seven PIK3CA exon 20 mutations (H1047R) and three exon 9 mutations (E545K). Analysis of tumors by ddPCR confirmed these mutations and identified five additional mutations. Presurgery plasma samples (n = 29) were then analyzed for PIK3CA mutations using ddPCR. Of the 15 PIK3CA mutations detected in tumors by ddPCR, 14 of the corresponding mutations were detected in presurgical ptDNA, whereas no mutations were found in plasma from patients with PIK3CA wild-type tumors (sensitivity 93.3%, specificity 100%). Ten patients with mutation-positive ptDNA presurgery had ddPCR analysis of postsurgery plasma, with five patients having detectable ptDNA postsurgery. Conclusions: This prospective study demonstrates accurate mutation detection in tumor tissues using ddPCR, and that ptDNA can be detected in blood before and after surgery in patients with early-stage breast cancer. Future studies can now address whether ptDNA detected after surgery identifies patients at risk for recurrence, which could guide chemotherapy decisions for individual patients.

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A08	`01`	`1`	`ENG`	`@1 Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer`
A11	`01`	`1`		`@1 BEAVER (Julia A.)`
A11	`02`	`1`		`@1 JELOVAC (Danijela)`
A11	`03`	`1`		`@1 BALUKRISHNA (Sasidharan)`
A11	`04`	`1`		`@1 COCHRAN (Rory L.)`
A11	`05`	`1`		`@1 CROESSMANN (Sarah)`
A11	`06`	`1`		`@1 ZABRANSKY (Daniel J.)`
A11	`07`	`1`		`@1 HONG YUEN WONG`
A11	`08`	`1`		`@1 TORO (Patricia Valda)`
A11	`09`	`1`		`@1 CIDADO (Justin)`
A11	`10`	`1`		`@1 BLAIR (Brian G.)`
A11	`11`	`1`		`@1 CHU (David)`
A11	`12`	`1`		`@1 BURNS (Timothy)`
A11	`13`	`1`		`@1 HIGGINS (Michaela J.)`
A11	`14`	`1`		`@1 STEARNS (Vered)`
A11	`15`	`1`		`@1 JACOBS (Lisa)`
A11	`16`	`1`		`@1 HABIBI (Mehran)`
A11	`17`	`1`		`@1 LANGE (Julie)`
A11	`18`	`1`		`@1 HURLEY (Paula J.)`
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A11	`20`	`1`		`@1 VANDENBERG (Dustin A.)`
A11	`21`	`1`		`@1 KESSLER (Jill)`
A11	`22`	`1`		`@1 JETER (Stacie)`
A11	`23`	`1`		`@1 SAMUELS (Michael L.)`
A11	`24`	`1`		`@1 MAAR (Dianna)`
A11	`25`	`1`		`@1 COPE (Leslie)`
A11	`26`	`1`		`@1 CIMINO-MATHEWS (Ashley)`
A11	`27`	`1`		`@1 ARGANI (Pedram)`
A11	`28`	`1`		`@1 WOLFF (Antonio C.)`
A11	`29`	`1`		`@1 BEN HO PARK`
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A14	`02`			`@1 University of Pittsburgh Hillman Cancer Center @2 Pittsburgh, Pennsylvania @3 USA @Z 12 aut.`
A14	`03`			`@1 Massachusetts General Hospital @2 Boston @3 USA @Z 13 aut.`
A14	`04`			`@1 RainDance Technologies @2 Billerica, Massachusetts @3 USA @Z 23 aut.`
A14	`05`			`@1 Bio-Rad Laboratories, Digital Biology Center @2 Pleasanton, California @3 USA @Z 24 aut.`
A14	`06`			`@1 Christian Medical College Vellore @2 Tamil Nadu @3 IND @Z 3 aut.`
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C01	`01`		`ENG`	@0 Purpose: Detecting circulating plasma tumor DNA (ptDNA) in patients with early-stage cancer has the potential to change how oncologists recommend systemic therapies for solid tumors after surgery. Droplet digital polymerase chain reaction (ddPCR) is a novel sensitive and specific platform for mutation detection. Experimental Design: In this prospective study, primary breast tumors and matched pre- and postsurgery blood samples were collected from patients with early-stage breast cancer (n = 29). Tumors (n = 30) were analyzed by Sanger sequencing for common PIK3CA mutations, and DNA from these tumors and matched plasma were then analyzed for PIK3CA mutations using ddPCR. Results: Sequencing of tumors identified seven PIK3CA exon 20 mutations (H1047R) and three exon 9 mutations (E545K). Analysis of tumors by ddPCR confirmed these mutations and identified five additional mutations. Presurgery plasma samples (n = 29) were then analyzed for PIK3CA mutations using ddPCR. Of the 15 PIK3CA mutations detected in tumors by ddPCR, 14 of the corresponding mutations were detected in presurgical ptDNA, whereas no mutations were found in plasma from patients with PIK3CA wild-type tumors (sensitivity 93.3%, specificity 100%). Ten patients with mutation-positive ptDNA presurgery had ddPCR analysis of postsurgery plasma, with five patients having detectable ptDNA postsurgery. Conclusions: This prospective study demonstrates accurate mutation detection in tumor tissues using ddPCR, and that ptDNA can be detected in blood before and after surgery in patients with early-stage breast cancer. Future studies can now address whether ptDNA detected after surgery identifies patients at risk for recurrence, which could guide chemotherapy decisions for individual patients.
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N21				`@1 202`

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Le document en format XML

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<author><name sortKey="Cidado, Justin" sort="Cidado, Justin" uniqKey="Cidado J" first="Justin" last="Cidado">Justin Cidado</name>
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<author><name sortKey="Blair, Brian G" sort="Blair, Brian G" uniqKey="Blair B" first="Brian G." last="Blair">Brian G. Blair</name>
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<author><name sortKey="Chu, David" sort="Chu, David" uniqKey="Chu D" first="David" last="Chu">David Chu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins</s1>
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<author><name sortKey="Burns, Timothy" sort="Burns, Timothy" uniqKey="Burns T" first="Timothy" last="Burns">Timothy Burns</name>
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<author><name sortKey="Higgins, Michaela J" sort="Higgins, Michaela J" uniqKey="Higgins M" first="Michaela J." last="Higgins">Michaela J. Higgins</name>
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<author><name sortKey="Ben Ho Park" sort="Ben Ho Park" uniqKey="Ben Ho Park" last="Ben Ho Park">BEN HO PARK</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer</title>
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<author><name sortKey="Jeter, Stacie" sort="Jeter, Stacie" uniqKey="Jeter S" first="Stacie" last="Jeter">Stacie Jeter</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins</s1>
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<author><name sortKey="Cimino Mathews, Ashley" sort="Cimino Mathews, Ashley" uniqKey="Cimino Mathews A" first="Ashley" last="Cimino-Mathews">Ashley Cimino-Mathews</name>
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<series><title level="j" type="main">Clinical cancer research : (Print)</title>
<title level="j" type="abbreviated">Clin. cancer res. : (Print)</title>
<idno type="ISSN">1078-0432</idno>
<imprint><date when="2014">2014</date>
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<seriesStmt><title level="j" type="main">Clinical cancer research : (Print)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological fluid</term>
<term>Blood plasma</term>
<term>Breast cancer</term>
<term>DNA</term>
<term>Detection</term>
<term>Diagnosis</term>
<term>Early stage</term>
<term>Human</term>
<term>Malignant tumor</term>
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<front><div type="abstract" xml:lang="en">Purpose: Detecting circulating plasma tumor DNA (ptDNA) in patients with early-stage cancer has the potential to change how oncologists recommend systemic therapies for solid tumors after surgery. Droplet digital polymerase chain reaction (ddPCR) is a novel sensitive and specific platform for mutation detection. Experimental Design: In this prospective study, primary breast tumors and matched pre- and postsurgery blood samples were collected from patients with early-stage breast cancer (n = 29). Tumors (n = 30) were analyzed by Sanger sequencing for common PIK3CA mutations, and DNA from these tumors and matched plasma were then analyzed for PIK3CA mutations using ddPCR. Results: Sequencing of tumors identified seven PIK3CA exon 20 mutations (H1047R) and three exon 9 mutations (E545K). Analysis of tumors by ddPCR confirmed these mutations and identified five additional mutations. Presurgery plasma samples (n = 29) were then analyzed for PIK3CA mutations using ddPCR. Of the 15 PIK3CA mutations detected in tumors by ddPCR, 14 of the corresponding mutations were detected in presurgical ptDNA, whereas no mutations were found in plasma from patients with PIK3CA wild-type tumors (sensitivity 93.3%, specificity 100%). Ten patients with mutation-positive ptDNA presurgery had ddPCR analysis of postsurgery plasma, with five patients having detectable ptDNA postsurgery. Conclusions: This prospective study demonstrates accurate mutation detection in tumor tissues using ddPCR, and that ptDNA can be detected in blood before and after surgery in patients with early-stage breast cancer. Future studies can now address whether ptDNA detected after surgery identifies patients at risk for recurrence, which could guide chemotherapy decisions for individual patients.</div>
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<s5>01</s5>
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<fC03 i1="01" i2="X" l="ENG"><s0>Detection</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Detección</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Diagnostic</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Diagnosis</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Diagnóstico</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Tumeur maligne</s0>
<s2>NM</s2>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Malignant tumor</s0>
<s2>NM</s2>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Tumor maligno</s0>
<s2>NM</s2>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Cancer du sein</s0>
<s2>NM</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Breast cancer</s0>
<s2>NM</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Cáncer del pecho</s0>
<s2>NM</s2>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>DNA</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>DNA</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>DNA</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Liquide biologique</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Biological fluid</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Líquido biológico</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Plasma sanguin</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Blood plasma</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Plasma sanguíneo</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Homme</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Human</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Hombre</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Stade précoce</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Early stage</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Estadio precoz</s0>
<s5>09</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Cancer</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Cancer</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Cáncer</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Pathologie de la glande mammaire</s0>
<s2>NM</s2>
<s5>37</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Mammary gland diseases</s0>
<s2>NM</s2>
<s5>37</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Glándula mamaria patología</s0>
<s2>NM</s2>
<s5>37</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Pathologie du sein</s0>
<s2>NM</s2>
<s5>38</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Breast disease</s0>
<s2>NM</s2>
<s5>38</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Seno patología</s0>
<s2>NM</s2>
<s5>38</s5>
</fC07>
<fN21><s1>202</s1>
</fN21>
</pA>
</standard>
</inist>
</record>

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   |wiki=    Wicri/Amérique
   |area=    PittsburghV1
   |flux=    PascalFrancis
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   |type=    RBID
   |clé=     Pascal:14-0157621
   |texte=   Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer
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Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer

Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer

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English descriptors

Abstract

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