Variability of cytokine gene expression in intestinal tissue and the impact of normalization with the use of reference genes.
Identifieur interne : 003783 ( Ncbi/Merge ); précédent : 003782; suivant : 003784Variability of cytokine gene expression in intestinal tissue and the impact of normalization with the use of reference genes.
Auteurs : Ian Mcgowan [États-Unis] ; Laura Janocko [États-Unis] ; Shaun Burneisen [États-Unis] ; Anand Bhat [États-Unis] ; Nicola Richardson-Harman [États-Unis]Source :
- Cytokine [ 1096-0023 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Adulte, Adulte d'âge moyen, Biopsie, Chimiokines (génétique), Cytokines (génétique), Exactitude des données, Expression des gènes, Gènes essentiels, Humains, Muqueuse intestinale (immunologie), Muqueuse intestinale (métabolisme), Normes de référence, Rectum (immunologie), Rectum (métabolisme), Reproductibilité des résultats, Réaction de polymérisation en chaine en temps réel (), Sujet âgé, Sujet âgé de 80 ans ou plus, Volontaires sains.
- MESH :
- génétique : ARN messager, Chimiokines, Cytokines.
- immunologie : Muqueuse intestinale, Rectum.
- métabolisme : Muqueuse intestinale, Rectum.
- Adulte, Adulte d'âge moyen, Biopsie, Exactitude des données, Expression des gènes, Gènes essentiels, Humains, Normes de référence, Reproductibilité des résultats, Réaction de polymérisation en chaine en temps réel, Sujet âgé, Sujet âgé de 80 ans ou plus, Volontaires sains.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Biopsy, Chemokines (genetics), Cytokines (genetics), Data Accuracy, Gene Expression, Genes, Essential, Healthy Volunteers, Humans, Intestinal Mucosa (immunology), Intestinal Mucosa (metabolism), Middle Aged, RNA, Messenger (genetics), Real-Time Polymerase Chain Reaction (methods), Rectum (immunology), Rectum (metabolism), Reference Standards, Reproducibility of Results.
- MESH :
- chemical , genetics : Chemokines, Cytokines, RNA, Messenger.
- immunology : Intestinal Mucosa, Rectum.
- metabolism : Intestinal Mucosa, Rectum.
- methods : Real-Time Polymerase Chain Reaction.
- Adult, Aged, Aged, 80 and over, Biopsy, Data Accuracy, Gene Expression, Genes, Essential, Healthy Volunteers, Humans, Middle Aged, Reference Standards, Reproducibility of Results.
Abstract
To determine the intra- and inter-subject variability of mucosal cytokine gene expression in rectal biopsies from healthy volunteers and to screen cytokine and chemokine mRNA as potential biomarkers of mucosal inflammation.
DOI: 10.1016/j.cyto.2014.08.010
PubMed: 25265568
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pubmed:25265568Le document en format XML
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xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Biopsy</term><term>Chemokines (genetics)</term><term>Cytokines (genetics)</term><term>Data Accuracy</term><term>Gene Expression</term><term>Genes, Essential</term><term>Healthy Volunteers</term><term>Humans</term><term>Intestinal Mucosa (immunology)</term><term>Intestinal Mucosa (metabolism)</term><term>Middle Aged</term><term>RNA, Messenger (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Rectum (immunology)</term><term>Rectum (metabolism)</term><term>Reference Standards</term><term>Reproducibility of Results</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Biopsie</term><term>Chimiokines (génétique)</term><term>Cytokines (génétique)</term><term>Exactitude des données</term><term>Expression des gènes</term><term>Gènes essentiels</term><term>Humains</term><term>Muqueuse intestinale (immunologie)</term><term>Muqueuse intestinale (métabolisme)</term><term>Normes de référence</term><term>Rectum (immunologie)</term><term>Rectum (métabolisme)</term><term>Reproductibilité des résultats</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Volontaires sains</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chemokines</term><term>Cytokines</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Chimiokines</term><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Muqueuse intestinale</term><term>Rectum</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Intestinal Mucosa</term><term>Rectum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Intestinal Mucosa</term><term>Rectum</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Muqueuse intestinale</term><term>Rectum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Biopsy</term><term>Data Accuracy</term><term>Gene Expression</term><term>Genes, Essential</term><term>Healthy Volunteers</term><term>Humans</term><term>Middle Aged</term><term>Reference Standards</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Biopsie</term><term>Exactitude des données</term><term>Expression des gènes</term><term>Gènes essentiels</term><term>Humains</term><term>Normes de référence</term><term>Reproductibilité des résultats</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Volontaires sains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To determine the intra- and inter-subject variability of mucosal cytokine gene expression in rectal biopsies from healthy volunteers and to screen cytokine and chemokine mRNA as potential biomarkers of mucosal inflammation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25265568</PMID><DateCreated><Year>2014</Year><Month>12</Month><Day>03</Day></DateCreated><DateCompleted><Year>2016</Year><Month>02</Month><Day>01</Day></DateCompleted><DateRevised><Year>2014</Year><Month>12</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0023</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Cytokine</Title><ISOAbbreviation>Cytokine</ISOAbbreviation></Journal><ArticleTitle>Variability of cytokine gene expression in intestinal tissue and the impact of normalization with the use of reference genes.</ArticleTitle><Pagination><MedlinePgn>81-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cyto.2014.08.010</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1043-4666(14)00530-4</ELocationID><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To determine the intra- and inter-subject variability of mucosal cytokine gene expression in rectal biopsies from healthy volunteers and to screen cytokine and chemokine mRNA as potential biomarkers of mucosal inflammation.</AbstractText><AbstractText Label="DESIGN AND METHODS" NlmCategory="METHODS">Rectal biopsies were collected from 8 participants (3 biopsies per participant) and 1 additional participant (10 biopsies). Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to quantify IL-1β, IL-6, IL-12p40, IL-8, IFN-γ, MIP-1α, MIP-1β, RANTES, and TNF-α gene expression in the rectal tissue. The intra-assay, inter-biopsy and inter-subject variance was measured in the eight participants. Bootstrap re-sampling of the biopsy measurements was performed to determine the accuracy of gene expression data obtained for 10 biopsies obtained from one participant. Cytokines were both non-normalized and normalized using four reference genes (GAPDH, β-actin, β2 microglobulin, and CD45).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Cytokine measurement accuracy was increased with the number of biopsy samples, per person; four biopsies were typically needed to produce a mean result within a 95% confidence interval of the subject's cytokine level approximately 80% of the time. Intra-assay precision (% geometric standard deviation) ranged between 8.2 and 96.9 with high variance between patients and even between different biopsies from the same patient. Variability was not greatly reduced with the use of reference genes to normalize data.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The number of biopsy samples required to provide an accurate result varied by target although 4 biopsy samples per subject and timepoint, provided for >77% accuracy across all targets tested. Biopsies within the same subjects and between subjects had similar levels of variance while variance within a biopsy (intra-assay) was generally lower. Normalization of inflammatory cytokines against reference genes failed to consistently reduce variance. The accuracy and reliability of mRNA expression of inflammatory cytokines will set a ceiling on the ability of these measures to predict mucosal inflammation. Techniques to reduce variability should be developed within a larger cohort of individuals before normative reference values can be validated.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>McGowan</LastName><ForeName>Ian</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Magee-Womens Research Institute, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: imcgowan@pitt.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Janocko</LastName><ForeName>Laura</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Magee-Womens Research Institute, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burneisen</LastName><ForeName>Shaun</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Magee-Womens Research Institute, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bhat</LastName><ForeName>Anand</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Case Western Reserve University at MetroHealth Medical Center, Cleveland, OH, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Richardson-Harman</LastName><ForeName>Nicola</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Alpha StatConsult, LLC, Damascus, MD, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>5R01HD059533</GrantID><Acronym>HD</Acronym><Agency>NICHD NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>09</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cytokine</MedlineTA><NlmUniqueID>9005353</NlmUniqueID><ISSNLinking>1043-4666</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018925">Chemokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001706" MajorTopicYN="N">Biopsy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018925" MajorTopicYN="N">Chemokines</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000068598" MajorTopicYN="N">Data Accuracy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020043" MajorTopicYN="N">Genes, Essential</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D064368" MajorTopicYN="N">Healthy Volunteers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007413" MajorTopicYN="N">Intestinal Mucosa</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012007" MajorTopicYN="N">Rectum</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012015" MajorTopicYN="N">Reference Standards</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Gut-associated lymphoid tissue (GALT)</Keyword><Keyword MajorTopicYN="N">Housekeeping genes</Keyword><Keyword MajorTopicYN="N">Intestinal cytokine gene expression</Keyword><Keyword MajorTopicYN="N">Reference genes</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>04</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>07</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>08</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>9</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>9</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25265568</ArticleId><ArticleId IdType="pii">S1043-4666(14)00530-4</ArticleId><ArticleId IdType="doi">10.1016/j.cyto.2014.08.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>Ohio</li><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Mcgowan, Ian" sort="Mcgowan, Ian" uniqKey="Mcgowan I" first="Ian" last="Mcgowan">Ian Mcgowan</name></region><name sortKey="Bhat, Anand" sort="Bhat, Anand" uniqKey="Bhat A" first="Anand" last="Bhat">Anand Bhat</name><name sortKey="Burneisen, Shaun" sort="Burneisen, Shaun" uniqKey="Burneisen S" first="Shaun" last="Burneisen">Shaun Burneisen</name><name sortKey="Janocko, Laura" sort="Janocko, Laura" uniqKey="Janocko L" first="Laura" last="Janocko">Laura Janocko</name><name sortKey="Richardson Harman, Nicola" sort="Richardson Harman, Nicola" uniqKey="Richardson Harman N" first="Nicola" last="Richardson-Harman">Nicola Richardson-Harman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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