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High dynamic range proteome imaging with the structured illumination gel imager.

Identifieur interne : 002572 ( Ncbi/Curation ); précédent : 002571; suivant : 002573

High dynamic range proteome imaging with the structured illumination gel imager.

Auteurs : Phu T. Van [États-Unis] ; Victor Bass ; Dan Shiwarski ; Frederick Lanni ; Jonathan Minden

Source :

Electrophoresis [ 1522-2683 ] ; 2014.

RBID : pubmed:24935033

Descripteurs français

KwdFr :
- Limite de détection, Protéines (analyse), Protéome (analyse), Protéomique (), Traitement d'image par ordinateur, Éclairage, Électrophorèse bidimensionnelle sur gel ().
MESH :
- analyse : Protéines, Protéome.
- Limite de détection, Protéomique, Traitement d'image par ordinateur, Éclairage, Électrophorèse bidimensionnelle sur gel.

English descriptors

KwdEn :
- Electrophoresis, Gel, Two-Dimensional (methods), Image Processing, Computer-Assisted, Lighting, Limit of Detection, Proteins (analysis), Proteome (analysis), Proteomics (methods).
MESH :
- chemical , analysis : Proteins, Proteome.
- methods : Electrophoresis, Gel, Two-Dimensional, Proteomics.
- Image Processing, Computer-Assisted, Lighting, Limit of Detection.

Abstract

A current challenge for proteomics is detecting proteins over the large concentration ranges found in complex biological samples such as whole-cell extracts. Currently, no unbiased, whole-proteome analysis scheme is capable of detecting the full range of cellular proteins. This is due in part to the limited dynamic range of the detectors used to sense proteins or peptides. We present a new technology, structured illumination (SI) gel imager, which detects fluorescently labeled proteins in electrophoretic gels over a 1 000 000-fold concentration range. SI uses computer-generated masks to attenuate the illumination of highly abundant proteins, allowing for long exposures of low-abundance proteins, thus avoiding detector saturation. A series of progressively masked gel images are assembled into a single, very high dynamic range image. We demonstrate that the SI imager can detect proteins over a concentration range of approximately 1 000 000-fold, making it a useful tool for comprehensive, unbiased proteome-wide surveys.

DOI: 10.1002/elps.201400126
PubMed: 24935033

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Le document en format XML

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<author><name sortKey="Van, Phu T" sort="Van, Phu T" uniqKey="Van P" first="Phu T" last="Van">Phu T. Van</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.</nlm:affiliation>
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<wicri:regionArea>Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
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<orgName type="university">Université Carnegie-Mellon</orgName>
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<author><name sortKey="Bass, Victor" sort="Bass, Victor" uniqKey="Bass V" first="Victor" last="Bass">Victor Bass</name>
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<author><name sortKey="Lanni, Frederick" sort="Lanni, Frederick" uniqKey="Lanni F" first="Frederick" last="Lanni">Frederick Lanni</name>
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<author><name sortKey="Shiwarski, Dan" sort="Shiwarski, Dan" uniqKey="Shiwarski D" first="Dan" last="Shiwarski">Dan Shiwarski</name>
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<term>Proteomics (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Limite de détection</term>
<term>Protéines (analyse)</term>
<term>Protéome (analyse)</term>
<term>Protéomique ()</term>
<term>Traitement d'image par ordinateur</term>
<term>Éclairage</term>
<term>Électrophorèse bidimensionnelle sur gel ()</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Proteins</term>
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<term>Protéome</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Proteomics</term>
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<term>Électrophorèse bidimensionnelle sur gel</term>
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<front><div type="abstract" xml:lang="en">A current challenge for proteomics is detecting proteins over the large concentration ranges found in complex biological samples such as whole-cell extracts. Currently, no unbiased, whole-proteome analysis scheme is capable of detecting the full range of cellular proteins. This is due in part to the limited dynamic range of the detectors used to sense proteins or peptides. We present a new technology, structured illumination (SI) gel imager, which detects fluorescently labeled proteins in electrophoretic gels over a 1 000 000-fold concentration range. SI uses computer-generated masks to attenuate the illumination of highly abundant proteins, allowing for long exposures of low-abundance proteins, thus avoiding detector saturation. A series of progressively masked gel images are assembled into a single, very high dynamic range image. We demonstrate that the SI imager can detect proteins over a concentration range of approximately 1 000 000-fold, making it a useful tool for comprehensive, unbiased proteome-wide surveys.</div>
</front>
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High dynamic range proteome imaging with the structured illumination gel imager.

High dynamic range proteome imaging with the structured illumination gel imager.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki