Genetic studies of human apolipoproteins: XIV. A simple agarose isoelectric focusing gel method for apolipoprotein E phenotyping
Identifieur interne : 00C631 ( Main/Merge ); précédent : 00C630; suivant : 00C632Genetic studies of human apolipoproteins: XIV. A simple agarose isoelectric focusing gel method for apolipoprotein E phenotyping
Auteurs : Mohammad I. Kamboh [États-Unis] ; Lori J. Kelly [États-Unis] ; Robert E. Ferrell [États-Unis]Source :
- ELECTROPHORESIS [ 0173-0835 ] ; 1990.
Descripteurs français
- Wicri :
- topic : Sciences médicales.
English descriptors
- KwdEn :
- Agarose, Agarose isoelectric, Alkaline phosphatase, Allele, Allele frequencies, Allele product, Cellulose acetate membrane, Clear background, Common phenotypes, Continuous flow microdialysis system, Conventional electrophoresis, Deionized water, Epidemiological studies, Erythrocyte acid phosphatase, Filter paper, General population, High background, Isoelectric, Legal medicine, Major bands, Medical science, Nitrocellulose filter, Phenotype, Phenotyping, Plasma samples, Polyacrylamide, Room temperature, Shiga university, Sialylated bands, Vivo catabolism, White populations.
- Teeft :
- Agarose, Agarose isoelectric, Alkaline phosphatase, Allele, Allele frequencies, Allele product, Cellulose acetate membrane, Clear background, Common phenotypes, Continuous flow microdialysis system, Conventional electrophoresis, Deionized water, Epidemiological studies, Erythrocyte acid phosphatase, Filter paper, General population, High background, Isoelectric, Legal medicine, Major bands, Medical science, Nitrocellulose filter, Phenotype, Phenotyping, Plasma samples, Polyacrylamide, Room temperature, Shiga university, Sialylated bands, Vivo catabolism, White populations.
Abstract
A new and simplified procedure is described for apolipoprotein E (APO E) phenotyping from native plasma or serum samples. Diluted or dialyzed samples are separated on agarose isoelectric focusing gels followed by protein blotting on nitrocellulose membranes. APO E banding patterns are localized immunologically using polyclonal goat anti‐APO E antiserum as the primary antibody and rabbit anti‐goat IgG conjugated with alkaline phosphatase as the secondary antibody. The method was used in parallel with our previously described polyacrylamide gel system to screen 110 unrelated and healthy US whites. Both gel systems gave identical APO E phenotypes, and allele frequencies were comparable with reported US white values. This simplified method can be used on a large number of population and clinical samples with minimum cost and effort.
Url:
DOI: 10.1002/elps.1150110408
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A simple agarose isoelectric focusing gel method for apolipoprotein E phenotyping</title><author><name sortKey="Kamboh, Mohammad I" sort="Kamboh, Mohammad I" uniqKey="Kamboh M" first="Mohammad I." last="Kamboh">Mohammad I. Kamboh</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Human Genetics Graduate School of Public Health University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation><affiliation wicri:level="4"><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Correspondence address: Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Kelly, Lori J" sort="Kelly, Lori J" uniqKey="Kelly L" first="Lori J." last="Kelly">Lori J. Kelly</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Human Genetics Graduate School of Public Health University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Ferrell, Robert E" sort="Ferrell, Robert E" uniqKey="Ferrell R" first="Robert E." last="Ferrell">Robert E. Ferrell</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Human Genetics Graduate School of Public Health University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">ELECTROPHORESIS</title><title level="j" type="alt">ELECTROPHORESIS</title><idno type="ISSN">0173-0835</idno><idno type="eISSN">1522-2683</idno><imprint><biblScope unit="vol">11</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="314">314</biblScope><biblScope unit="page" to="318">318</biblScope><biblScope unit="page-count">5</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1990">1990</date></imprint><idno type="ISSN">0173-0835</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0173-0835</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agarose</term><term>Agarose isoelectric</term><term>Alkaline phosphatase</term><term>Allele</term><term>Allele frequencies</term><term>Allele product</term><term>Cellulose acetate membrane</term><term>Clear background</term><term>Common phenotypes</term><term>Continuous flow microdialysis system</term><term>Conventional electrophoresis</term><term>Deionized water</term><term>Epidemiological studies</term><term>Erythrocyte acid phosphatase</term><term>Filter paper</term><term>General population</term><term>High background</term><term>Isoelectric</term><term>Legal medicine</term><term>Major bands</term><term>Medical science</term><term>Nitrocellulose filter</term><term>Phenotype</term><term>Phenotyping</term><term>Plasma samples</term><term>Polyacrylamide</term><term>Room temperature</term><term>Shiga university</term><term>Sialylated bands</term><term>Vivo catabolism</term><term>White populations</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Agarose</term><term>Agarose isoelectric</term><term>Alkaline phosphatase</term><term>Allele</term><term>Allele frequencies</term><term>Allele product</term><term>Cellulose acetate membrane</term><term>Clear background</term><term>Common phenotypes</term><term>Continuous flow microdialysis system</term><term>Conventional electrophoresis</term><term>Deionized water</term><term>Epidemiological studies</term><term>Erythrocyte acid phosphatase</term><term>Filter paper</term><term>General population</term><term>High background</term><term>Isoelectric</term><term>Legal medicine</term><term>Major bands</term><term>Medical science</term><term>Nitrocellulose filter</term><term>Phenotype</term><term>Phenotyping</term><term>Plasma samples</term><term>Polyacrylamide</term><term>Room temperature</term><term>Shiga university</term><term>Sialylated bands</term><term>Vivo catabolism</term><term>White populations</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Sciences médicales</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new and simplified procedure is described for apolipoprotein E (APO E) phenotyping from native plasma or serum samples. Diluted or dialyzed samples are separated on agarose isoelectric focusing gels followed by protein blotting on nitrocellulose membranes. APO E banding patterns are localized immunologically using polyclonal goat anti‐APO E antiserum as the primary antibody and rabbit anti‐goat IgG conjugated with alkaline phosphatase as the secondary antibody. The method was used in parallel with our previously described polyacrylamide gel system to screen 110 unrelated and healthy US whites. Both gel systems gave identical APO E phenotypes, and allele frequencies were comparable with reported US white values. This simplified method can be used on a large number of population and clinical samples with minimum cost and effort.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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