Time-Insensitive Fluorescent Sensor for Human Serum Albumin and Its Unusual Red Shift
Identifieur interne : 000B63 ( Main/Exploration ); précédent : 000B62; suivant : 000B64Time-Insensitive Fluorescent Sensor for Human Serum Albumin and Its Unusual Red Shift
Auteurs : Sara E. Smith ; Jessica M. Williams ; Shin Ando ; Kazunori KoideSource :
- Analytical Chemistry [ 0003-2700 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Fluorescent Dyes, Serum Albumin.
- Humans, Spectrophotometry, Ultraviolet.
Abstract
The concentration of human serum albumin (HSA) indicates the health state of individuals and is routinely measured by UV spectroscopy with bromocresol. However, this method tends to overestimate HSA, and more critically, depends highly on the timing, in seconds, of the measurements. Here, we report an analog of 2′,7′-dichlorofluorescein that can be used as a fluorescent sensor to quantify HSA in human sera. The accuracy of this new method proved superior to that of bromocresol when an international standard serum sample was analyzed. This method is more convenient than the bromocresol method because it allows for fluorescence measurements during a >15 min period. Colorimetric analysis was also performed to further investigate the effects of the binding of the sensor to HSA. These spectroscopic studies suggest that absorption and emission changes upon HSA binding may be due to the dehydration of the dye and/or stabilization of the tritylic cation species.
Url:
DOI: 10.1021/ac5001256
PubMed: 24527887
PubMed Central: 3983026
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000278
- to stream Pmc, to step Curation: 000278
- to stream Pmc, to step Checkpoint: 000221
- to stream PubMed, to step Corpus: 003878
- to stream PubMed, to step Curation: 003852
- to stream PubMed, to step Checkpoint: 003852
- to stream Ncbi, to step Merge: 001131
- to stream Ncbi, to step Curation: 001131
- to stream Ncbi, to step Checkpoint: 001131
- to stream Main, to step Merge: 000B75
- to stream Main, to step Curation: 000B63
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift</title><author><name sortKey="Smith, Sara E" sort="Smith, Sara E" uniqKey="Smith S" first="Sara E." last="Smith">Sara E. Smith</name></author><author><name sortKey="Williams, Jessica M" sort="Williams, Jessica M" uniqKey="Williams J" first="Jessica M." last="Williams">Jessica M. Williams</name></author><author><name sortKey="Ando, Shin" sort="Ando, Shin" uniqKey="Ando S" first="Shin" last="Ando">Shin Ando</name></author><author><name sortKey="Koide, Kazunori" sort="Koide, Kazunori" uniqKey="Koide K" first="Kazunori" last="Koide">Kazunori Koide</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24527887</idno><idno type="pmc">3983026</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983026</idno><idno type="RBID">PMC:3983026</idno><idno type="doi">10.1021/ac5001256</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000278</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000278</idno><idno type="wicri:Area/Pmc/Curation">000278</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000278</idno><idno type="wicri:Area/Pmc/Checkpoint">000221</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000221</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">003878</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003878</idno><idno type="wicri:Area/PubMed/Curation">003852</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003852</idno><idno type="wicri:Area/PubMed/Checkpoint">003852</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003852</idno><idno type="wicri:Area/Ncbi/Merge">001131</idno><idno type="wicri:Area/Ncbi/Curation">001131</idno><idno type="wicri:Area/Ncbi/Checkpoint">001131</idno><idno type="wicri:doubleKey">0003-2700:2014:Smith S:time:insensitive:fluorescent</idno><idno type="wicri:Area/Main/Merge">000B75</idno><idno type="wicri:Area/Main/Curation">000B63</idno><idno type="wicri:Area/Main/Exploration">000B63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift</title><author><name sortKey="Smith, Sara E" sort="Smith, Sara E" uniqKey="Smith S" first="Sara E." last="Smith">Sara E. Smith</name></author><author><name sortKey="Williams, Jessica M" sort="Williams, Jessica M" uniqKey="Williams J" first="Jessica M." last="Williams">Jessica M. Williams</name></author><author><name sortKey="Ando, Shin" sort="Ando, Shin" uniqKey="Ando S" first="Shin" last="Ando">Shin Ando</name></author><author><name sortKey="Koide, Kazunori" sort="Koide, Kazunori" uniqKey="Koide K" first="Kazunori" last="Koide">Kazunori Koide</name></author></analytic><series><title level="j">Analytical Chemistry</title><idno type="ISSN">0003-2700</idno><idno type="eISSN">1520-6882</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fluorescent Dyes (chemistry)</term><term>Humans</term><term>Serum Albumin (chemistry)</term><term>Spectrophotometry, Ultraviolet</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Colorants fluorescents ()</term><term>Humains</term><term>Spectrophotométrie UV</term><term>Sérumalbumine ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fluorescent Dyes</term><term>Serum Albumin</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Spectrophotometry, Ultraviolet</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Colorants fluorescents</term><term>Humains</term><term>Spectrophotométrie UV</term><term>Sérumalbumine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p content-type="toc-graphic"><graphic xlink:href="ac-2014-001256_0006" id="ab-d31e125"></graphic></p><p>The concentration
of human serum albumin (HSA) indicates the health
state of individuals and is routinely measured by UV spectroscopy
with bromocresol. However, this method tends to overestimate HSA,
and more critically, depends highly on the timing, in seconds, of
the measurements. Here, we report an analog of 2′,7′-dichlorofluorescein
that can be used as a fluorescent sensor to quantify HSA in human
sera. The accuracy of this new method proved superior to that of bromocresol
when an international standard serum sample was analyzed. This method
is more convenient than the bromocresol method because it allows for
fluorescence measurements during a >15 min period. Colorimetric
analysis
was also performed to further investigate the effects of the binding
of the sensor to HSA. These spectroscopic studies suggest that absorption
and emission changes upon HSA binding may be due to the dehydration
of the dye and/or stabilization of the tritylic cation species.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Dockal, M" uniqKey="Dockal M">M. Dockal</name></author><author><name sortKey="Carter, D C" uniqKey="Carter D">D. C. Carter</name></author><author><name sortKey="Ruker, F" uniqKey="Ruker F">F. Ruker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bourdon, E" uniqKey="Bourdon E">E. Bourdon</name></author><author><name sortKey="Loreau, N" uniqKey="Loreau N">N. Loreau</name></author><author><name sortKey="Blache, D" uniqKey="Blache D">D. Blache</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greipp, P R" uniqKey="Greipp P">P. R. Greipp</name></author><author><name sortKey="San Miguel, J" uniqKey="San Miguel J">J. San Miguel</name></author><author><name sortKey="Durie, B G M" uniqKey="Durie B">B. G. M. Durie</name></author><author><name sortKey="Crowley, J J" uniqKey="Crowley J">J. J. Crowley</name></author><author><name sortKey="Barlogie, B" uniqKey="Barlogie B">B. Barlogie</name></author><author><name sortKey="Blade, J" uniqKey="Blade J">J. Blade</name></author><author><name sortKey="Boccadoro, M" uniqKey="Boccadoro M">M. Boccadoro</name></author><author><name sortKey="Child, J A" uniqKey="Child J">J. A. Child</name></author><author><name sortKey="Harousseau, J L" uniqKey="Harousseau J">J. L. Harousseau</name></author><author><name sortKey="Kyle, R A" uniqKey="Kyle R">R. A. Kyle</name></author><author><name sortKey="Lahuerta, J J" uniqKey="Lahuerta J">J. J. Lahuerta</name></author><author><name sortKey="Ludwig, H" uniqKey="Ludwig H">H. Ludwig</name></author><author><name sortKey="Morgan, G" uniqKey="Morgan G">G. Morgan</name></author><author><name sortKey="Powles, R" uniqKey="Powles R">R. Powles</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K. Shimizu</name></author><author><name sortKey="Shustik, C" uniqKey="Shustik C">C. Shustik</name></author><author><name sortKey="Sonneveld, P" uniqKey="Sonneveld P">P. Sonneveld</name></author><author><name sortKey="Tosi, P" uniqKey="Tosi P">P. Tosi</name></author><author><name sortKey="Turesson, I" uniqKey="Turesson I">I. Turesson</name></author><author><name sortKey="Westin, J" uniqKey="Westin J">J. Westin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corti, M C" uniqKey="Corti M">M. C. Corti</name></author><author><name sortKey="Guralnik, J M" uniqKey="Guralnik J">J. M. Guralnik</name></author><author><name sortKey="Salive, M E" uniqKey="Salive M">M. E. Salive</name></author><author><name sortKey="Sorkin, J D" uniqKey="Sorkin J">J. D. Sorkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Llewellyn, D J" uniqKey="Llewellyn D">D. J. Llewellyn</name></author><author><name sortKey="Langa, K M" uniqKey="Langa K">K. M. Langa</name></author><author><name sortKey="Friedland, R P" uniqKey="Friedland R">R. P. Friedland</name></author><author><name sortKey="Lang, I A" uniqKey="Lang I">I. A. Lang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Danesh, J" uniqKey="Danesh J">J. Danesh</name></author><author><name sortKey="Collins, R" uniqKey="Collins R">R. Collins</name></author><author><name sortKey="Appleby, P" uniqKey="Appleby P">P. Appleby</name></author><author><name sortKey="Peto, R" uniqKey="Peto R">R. Peto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lyon, A W" uniqKey="Lyon A">A. W. Lyon</name></author><author><name sortKey="Meinert, P" uniqKey="Meinert P">P. Meinert</name></author><author><name sortKey="Bruce, G A" uniqKey="Bruce G">G. A. Bruce</name></author><author><name sortKey="Laxdal, V A" uniqKey="Laxdal V">V. A. Laxdal</name></author><author><name sortKey="Salkie, M L" uniqKey="Salkie M">M. L. Salkie</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Rodkey, F L" uniqKey="Rodkey F">F. L. Rodkey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doumas, B T" uniqKey="Doumas B">B. T. Doumas</name></author><author><name sortKey="Watson, W A" uniqKey="Watson W">W. A. Watson</name></author><author><name sortKey="Biggs, H G" uniqKey="Biggs H">H. G. Biggs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Margarson, M P" uniqKey="Margarson M">M. P. Margarson</name></author><author><name sortKey="Soni, N" uniqKey="Soni N">N. Soni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Louderback, A" uniqKey="Louderback A">A. Louderback</name></author><author><name sortKey="Mealey, E H" uniqKey="Mealey E">E. H. Mealey</name></author><author><name sortKey="Taylor, N A" uniqKey="Taylor N">N. A. Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pinnell, A E" uniqKey="Pinnell A">A. E. Pinnell</name></author><author><name sortKey="Northam, B E" uniqKey="Northam B">B. E. Northam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doumas, B T" uniqKey="Doumas B">B. T. Doumas</name></author><author><name sortKey="Peters, T" uniqKey="Peters T">T. Peters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Webster, D" uniqKey="Webster D">D. Webster</name></author><author><name sortKey="Bignell, A H C" uniqKey="Bignell A">A. H. C. Bignell</name></author><author><name sortKey="Attwood, E C" uniqKey="Attwood E">E. C. Attwood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carfray, A" uniqKey="Carfray A">A. Carfray</name></author><author><name sortKey="Patel, K" uniqKey="Patel K">K. Patel</name></author><author><name sortKey="Whitaker, P" uniqKey="Whitaker P">P. Whitaker</name></author><author><name sortKey="Garrick, P" uniqKey="Garrick P">P. Garrick</name></author><author><name sortKey="Griffiths, G J" uniqKey="Griffiths G">G. J. Griffiths</name></author><author><name sortKey="Warwick, G L" uniqKey="Warwick G">G. L. Warwick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parikh, C" uniqKey="Parikh C">C. Parikh</name></author><author><name sortKey="Yalavarthy, R" uniqKey="Yalavarthy R">R. Yalavarthy</name></author><author><name sortKey="Gurevich, A" uniqKey="Gurevich A">A. Gurevich</name></author><author><name sortKey="Robinson, A" uniqKey="Robinson A">A. Robinson</name></author><author><name sortKey="Teitelbaum, I" uniqKey="Teitelbaum I">I. Teitelbaum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snozek, C L H" uniqKey="Snozek C">C. L. H. Snozek</name></author><author><name sortKey="Saenger, A K" uniqKey="Saenger A">A. K. Saenger</name></author><author><name sortKey="Greipp, P R" uniqKey="Greipp P">P. R. Greipp</name></author><author><name sortKey="Bryant, S C" uniqKey="Bryant S">S. C. Bryant</name></author><author><name sortKey="Kyle, R A" uniqKey="Kyle R">R. A. Kyle</name></author><author><name sortKey="Rajkumar, S V" uniqKey="Rajkumar S">S. V. Rajkumar</name></author><author><name sortKey="Katzmann, J A" uniqKey="Katzmann J">J. A. Katzmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keay, G" uniqKey="Keay G">G. Keay</name></author><author><name sortKey="Doxey, D L" uniqKey="Doxey D">D. L. Doxey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gustafsson, J E" uniqKey="Gustafsson J">J. E. Gustafsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De, M" uniqKey="De M">M. De</name></author><author><name sortKey="Rana, S" uniqKey="Rana S">S. Rana</name></author><author><name sortKey="Akpinar, H" uniqKey="Akpinar H">H. Akpinar</name></author><author><name sortKey="Miranda, O R" uniqKey="Miranda O">O. R. Miranda</name></author><author><name sortKey="Arvizo, R R" uniqKey="Arvizo R">R. R. Arvizo</name></author><author><name sortKey="Bunz, U H F" uniqKey="Bunz U">U. H. F. Bunz</name></author><author><name sortKey="Rotello, V M" uniqKey="Rotello V">V. M. Rotello</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miranda, O R" uniqKey="Miranda O">O. R. Miranda</name></author><author><name sortKey="Chen, H T" uniqKey="Chen H">H. T. Chen</name></author><author><name sortKey="You, C C" uniqKey="You C">C. C. You</name></author><author><name sortKey="Mortenson, D E" uniqKey="Mortenson D">D. E. Mortenson</name></author><author><name sortKey="Yang, X C" uniqKey="Yang X">X. C. Yang</name></author><author><name sortKey="Bunz, U H F" uniqKey="Bunz U">U. H. F. Bunz</name></author><author><name sortKey="Rotello, V M" uniqKey="Rotello V">V. M. Rotello</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cook, M P" uniqKey="Cook M">M. P. Cook</name></author><author><name sortKey="Ando, S" uniqKey="Ando S">S. Ando</name></author><author><name sortKey="Koide, K" uniqKey="Koide K">K. Koide</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garner, A L" uniqKey="Garner A">A. L. Garner</name></author><author><name sortKey="St Croix, C M" uniqKey="St Croix C">C. M. St Croix</name></author><author><name sortKey="Pitt, B R" uniqKey="Pitt B">B. R. Pitt</name></author><author><name sortKey="Leikauf, G D" uniqKey="Leikauf G">G. D. Leikauf</name></author><author><name sortKey="Ando, S" uniqKey="Ando S">S. Ando</name></author><author><name sortKey="Koide, K" uniqKey="Koide K">K. Koide</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koide, K" uniqKey="Koide K">K. Koide</name></author><author><name sortKey="Song, F L" uniqKey="Song F">F. L. Song</name></author><author><name sortKey="De Groh, E D" uniqKey="De Groh E">E. D. de Groh</name></author><author><name sortKey="Garner, A L" uniqKey="Garner A">A. L. Garner</name></author><author><name sortKey="Mitchell, V D" uniqKey="Mitchell V">V. D. Mitchell</name></author><author><name sortKey="Davidson, L A" uniqKey="Davidson L">L. A. Davidson</name></author><author><name sortKey="Hukriede, N A" uniqKey="Hukriede N">N. A. Hukriede</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ando, S" uniqKey="Ando S">S. Ando</name></author><author><name sortKey="Koide, K" uniqKey="Koide K">K. Koide</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagataki, S" uniqKey="Nagataki S">S. Nagataki</name></author><author><name sortKey="Matsunaga, I" uniqKey="Matsunaga I">I. Matsunaga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doumas, B T" uniqKey="Doumas B">B. T. Doumas</name></author><author><name sortKey="Watson, W A" uniqKey="Watson W">W. A. Watson</name></author><author><name sortKey="Biggs, H G" uniqKey="Biggs H">H. G. Biggs</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Mcqueen, P X0a D" uniqKey="Mcqueen P">P.
D. McQueen</name></author><author><name sortKey="Sagoo, S" uniqKey="Sagoo S">S. Sagoo</name></author><author><name sortKey="Yao, H" uniqKey="Yao H">H. Yao</name></author><author><name sortKey="Jockusch, R A" uniqKey="Jockusch R">R. A. Jockusch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yao, H" uniqKey="Yao H">H. Yao</name></author><author><name sortKey="Jockusch, R A" uniqKey="Jockusch R">R. A. Jockusch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cohen, M D" uniqKey="Cohen M">M. D. Cohen</name></author><author><name sortKey="Fischer, E" uniqKey="Fischer E">E. Fischer</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ando, Shin" sort="Ando, Shin" uniqKey="Ando S" first="Shin" last="Ando">Shin Ando</name><name sortKey="Koide, Kazunori" sort="Koide, Kazunori" uniqKey="Koide K" first="Kazunori" last="Koide">Kazunori Koide</name><name sortKey="Smith, Sara E" sort="Smith, Sara E" uniqKey="Smith S" first="Sara E." last="Smith">Sara E. Smith</name><name sortKey="Williams, Jessica M" sort="Williams, Jessica M" uniqKey="Williams J" first="Jessica M." last="Williams">Jessica M. Williams</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Amérique/explor/PittsburghV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B63 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B63 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Amérique
|area= PittsburghV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3983026
|texte= Time-Insensitive Fluorescent Sensor for Human Serum
Albumin and Its Unusual Red Shift
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24527887" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PittsburghV1
| This area was generated with Dilib version V0.6.38. |  |