Superparamagnetic iron oxide (SPIO) labeling efficiency and subsequent MRI tracking of native cell populations pertinent to pulmonary heart valve tissue engineering studies
Identifieur interne : 007959 ( Main/Exploration ); précédent : 007958; suivant : 007960Superparamagnetic iron oxide (SPIO) labeling efficiency and subsequent MRI tracking of native cell populations pertinent to pulmonary heart valve tissue engineering studies
Auteurs : Sharan Ramaswamy [États-Unis] ; Paul A. Schornack [États-Unis] ; Adam G. Smelko [États-Unis] ; Steven M. Boronyak [États-Unis] ; Julia Ivanova [États-Unis] ; John E. Mayer Jr [États-Unis] ; Michael S. Sacks [États-Unis]Source :
- NMR in Biomedicine [ 0952-3480 ] ; 2012-03.
Descripteurs français
- Wicri :
- topic : Droit d'auteur, Oxyde.
English descriptors
- KwdEn :
- Agar, Binding buffer, Biomed, Biomedical engineering, Bioreactor, Cell detection, Cell fate, Cell populations, Cell type, Cell types, Cell viability, Cellular, Cellular agar, Cellular imaging, Clinical studies, Clinical trials, Copyright, Electronic proceedings, Endosomal uptake, Entire stack, Full width, Gaussian excitation, Gradient echo acquisition, Gradient echo images, Gradient echo measurements, Heart valve application, Heart valve tissue engineering, Heart valve tissue engineering figure, Heart valve tissues, Heart valves, Higher concentration, Horizontal system, Human vecs, Human vsmcs, Hypointense, Hypointense regions, Imaging, Imaging experiments, Implantation, Intensity projection, Iron oxide, Iron oxide concentration, Iron oxide content, Iron oxide microparticles, Iron oxide particles, John wiley sons, Magnetic resonance imaging, Mechanical conditioning experiments, Medial linings, Mesenchymal, Microparticles, Migratory processes, Multislice gradient echo measurements, Muscle cells, National institutes, Native cell populations, Oxide, Pediatric population, Preliminary work, Previous work, Progenitor cells, Protamine, Protamine sulfate, Protamine sulfate complexed, Protamine sulfate concentration, Protamine sulfate concentrations, Prussian slides, Pulmonary artery, Pulmonary valves, Ramaswamy, Relaxation rate, Resonance imaging, Scaffold environment, Signal decay, Signal intensity, Smooth muscle cells, Spio, Spio microparticles, Spio particles, Spio uptake, Sulfate, Superparamagnetic, Superparamagnetic iron oxide, Superparamagnetic iron oxide nanoparticles, Temporal resolution, Tepv, Tepv implantation, Tepv scaffolds, Tepvs, Tissue engineering, Tissue formation, Unlabeled, Unlabeled cells, Unlabeled counterparts, Unlabeled vecs, Unlabeled vsmcs, Unstained slides, Uorescein isothiocyanate, Valve, Vascular, Vecs, Viability, Vivo, Voxels, Vsmc, Vsmcs.
- Teeft :
- Agar, Binding buffer, Biomed, Biomedical engineering, Bioreactor, Cell detection, Cell fate, Cell populations, Cell type, Cell types, Cell viability, Cellular, Cellular agar, Cellular imaging, Clinical studies, Clinical trials, Copyright, Electronic proceedings, Endosomal uptake, Entire stack, Full width, Gaussian excitation, Gradient echo acquisition, Gradient echo images, Gradient echo measurements, Heart valve application, Heart valve tissue engineering, Heart valve tissue engineering figure, Heart valve tissues, Heart valves, Higher concentration, Horizontal system, Human vecs, Human vsmcs, Hypointense, Hypointense regions, Imaging, Imaging experiments, Implantation, Intensity projection, Iron oxide, Iron oxide concentration, Iron oxide content, Iron oxide microparticles, Iron oxide particles, John wiley sons, Magnetic resonance imaging, Mechanical conditioning experiments, Medial linings, Mesenchymal, Microparticles, Migratory processes, Multislice gradient echo measurements, Muscle cells, National institutes, Native cell populations, Oxide, Pediatric population, Preliminary work, Previous work, Progenitor cells, Protamine, Protamine sulfate, Protamine sulfate complexed, Protamine sulfate concentration, Protamine sulfate concentrations, Prussian slides, Pulmonary artery, Pulmonary valves, Ramaswamy, Relaxation rate, Resonance imaging, Scaffold environment, Signal decay, Signal intensity, Smooth muscle cells, Spio, Spio microparticles, Spio particles, Spio uptake, Sulfate, Superparamagnetic, Superparamagnetic iron oxide, Superparamagnetic iron oxide nanoparticles, Temporal resolution, Tepv, Tepv implantation, Tepv scaffolds, Tepvs, Tissue engineering, Tissue formation, Unlabeled, Unlabeled cells, Unlabeled counterparts, Unlabeled vecs, Unlabeled vsmcs, Unstained slides, Uorescein isothiocyanate, Valve, Vascular, Vecs, Viability, Vivo, Voxels, Vsmc, Vsmcs.
Abstract
The intimal and medial linings of the pulmonary artery consist largely of vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs), respectively. The migration of these cell types to a potential tissue‐engineered pulmonary valve (TEPV) implant process is therefore of interest in understanding the valve remodeling process. Visualization and cell tracking by MRI, which employs hypointense contrast achievable through the use of superparamagnetic iron oxide (SPIO) microparticles to label cells, provides a method in which this can be studied. We investigated the SPIO labeling efficiency of human VECs and VSMCs, and used two‐ and three‐dimensional gradient echo sequences to track the migration of these cells in agar gel constructs. Protamine sulfate (4.5 µg/mL) was used to enhance SPIO uptake and was found to have no influence on cell viability or proliferation. MRI experiments were initially performed using a 9.4‐T scanner. The results demonstrated that the spatial positions of hypointense spots were relatively unchanged over 12 days. Subsequent MR experiments performed at 7 T demonstrated that three‐dimensional imaging provided the best spatial resolution to assess cell fate. R2* maps were bright in SPIO cell‐encapsulated gels in comparison with unlabeled counterparts. Signal voids were ruled out as hypointense regions owing to the smooth exponential decay of T2* in these voxels. As a next step, we intend to use the SPIO cell labeling and MR protocols established in this study to assess whether hemodynamic stresses will alter the vascular cell migratory patterns. These studies will shed light on the mechanisms of vascular remodeling after TEPV implantation. Copyright © 2011 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/nbm.1642
Affiliations:
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<term>Heart valve tissue engineering figure</term>
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<term>Horizontal system</term>
<term>Human vecs</term>
<term>Human vsmcs</term>
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<term>Hypointense regions</term>
<term>Imaging</term>
<term>Imaging experiments</term>
<term>Implantation</term>
<term>Intensity projection</term>
<term>Iron oxide</term>
<term>Iron oxide concentration</term>
<term>Iron oxide content</term>
<term>Iron oxide microparticles</term>
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<term>John wiley sons</term>
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<term>Microparticles</term>
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<term>Pediatric population</term>
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<term>Unlabeled cells</term>
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<front><div type="abstract" xml:lang="en">The intimal and medial linings of the pulmonary artery consist largely of vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs), respectively. The migration of these cell types to a potential tissue‐engineered pulmonary valve (TEPV) implant process is therefore of interest in understanding the valve remodeling process. Visualization and cell tracking by MRI, which employs hypointense contrast achievable through the use of superparamagnetic iron oxide (SPIO) microparticles to label cells, provides a method in which this can be studied. We investigated the SPIO labeling efficiency of human VECs and VSMCs, and used two‐ and three‐dimensional gradient echo sequences to track the migration of these cells in agar gel constructs. Protamine sulfate (4.5 µg/mL) was used to enhance SPIO uptake and was found to have no influence on cell viability or proliferation. MRI experiments were initially performed using a 9.4‐T scanner. The results demonstrated that the spatial positions of hypointense spots were relatively unchanged over 12 days. Subsequent MR experiments performed at 7 T demonstrated that three‐dimensional imaging provided the best spatial resolution to assess cell fate. R2* maps were bright in SPIO cell‐encapsulated gels in comparison with unlabeled counterparts. Signal voids were ruled out as hypointense regions owing to the smooth exponential decay of T2* in these voxels. As a next step, we intend to use the SPIO cell labeling and MR protocols established in this study to assess whether hemodynamic stresses will alter the vascular cell migratory patterns. These studies will shed light on the mechanisms of vascular remodeling after TEPV implantation. Copyright © 2011 John Wiley & Sons, Ltd.</div>
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<name sortKey="Ramaswamy, Sharan" sort="Ramaswamy, Sharan" uniqKey="Ramaswamy S" first="Sharan" last="Ramaswamy">Sharan Ramaswamy</name>
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