Banff Initiative for Quality Assurance in Transplantation (BIFQUIT): Reproducibility of Polyomavirus Immunohistochemistry in Kidney Allografts
Identifieur interne : 003C63 ( Main/Exploration ); précédent : 003C62; suivant : 003C64Banff Initiative for Quality Assurance in Transplantation (BIFQUIT): Reproducibility of Polyomavirus Immunohistochemistry in Kidney Allografts
Auteurs : Benjamin Adam [Canada] ; Parmjeet Randhawa [États-Unis] ; Samantha Chan [Canada] ; Gang Zeng [États-Unis] ; Heinz Regele [Autriche] ; Yael B. Kushner [États-Unis] ; Robert B. Colvin [États-Unis] ; Jeff Reeve [Canada] ; Michael Mengel [Canada]Source :
- American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons [ 1600-6135 ] ; 2014.
Abstract
Immunohistochemistry is the gold standard for diagnosing (positive versus negative) polyomavirus BK (BKV) nephropathy and has the potential for disease staging based on staining intensity and quantification of infected cells. This multicenter trial evaluated the reproducibility of BKV immunohistochemistry among 81 pathologists at 60 institutions. Participants stained tissue microarray slides and scored them for staining intensity and percentage of positive nuclei. Staining protocol details and evaluation scores were collected online. Slides were returned for centralized panel re-evaluation and kappa statistics were calculated. Individual assessment of staining intensity and percentage was more reproducible than combined scoring. Inter-institutional reproducibility was moderate for staining intensity (κ=0.49) and percentage (κ=0.42), fair for combined (κ=0.25), and best for simple positive/negative scoring (κ=0.63). Inter-observer reproducibility was substantial for intensity (κ=0.74), percentage (κ=0.66), and positive/negative (κ=0.67), and moderate for combined scoring (κ=0.43). Inter-laboratory reproducibility was fair for intensity (κ=0.37), percentage (κ=0.40), and combined (κ=0.24), but substantial for positive/negative scoring (κ=0.78). BKV RNA copies/cell correlated with staining intensity (r=0.56) and percentage (r=0.62). These results indicate that BKV immunohistochemistry is reproducible between observers but scoring should be simplified to a single-feature schema. Standardization of tissue processing and staining protocols would further improve inter-laboratory reproducibility.
Url:
DOI: 10.1111/ajt.12794
PubMed: 25091177
PubMed Central: 4194133
Affiliations:
- Autriche, Canada, États-Unis
- Massachusetts, Pennsylvanie, Vienne (Autriche)
- Boston, Pittsburgh, Vienne (Autriche)
- Université de Pittsburgh
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Le document en format XML
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Kushner</name><affiliation wicri:level="3"><nlm:aff id="A5">Department of Pathology, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Colvin, Robert B" sort="Colvin, Robert B" uniqKey="Colvin R" first="Robert B." last="Colvin">Robert B. Colvin</name><affiliation wicri:level="3"><nlm:aff id="A6">Department of Pathology, Harvard Medical School and Massachusetts General Hospital, Boston, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Harvard Medical School and Massachusetts General Hospital, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Reeve, Jeff" sort="Reeve, Jeff" uniqKey="Reeve J" first="Jeff" last="Reeve">Jeff Reeve</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton</wicri:regionArea><wicri:noRegion>Edmonton</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton</wicri:regionArea><wicri:noRegion>Edmonton</wicri:noRegion></affiliation></author><author><name sortKey="Mengel, Michael" sort="Mengel, Michael" uniqKey="Mengel M" first="Michael" last="Mengel">Michael Mengel</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton</wicri:regionArea><wicri:noRegion>Edmonton</wicri:noRegion></affiliation></author></analytic><series><title level="j">American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons</title><idno type="ISSN">1600-6135</idno><idno type="eISSN">1600-6143</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Immunohistochemistry is the gold standard for diagnosing (positive versus negative) polyomavirus BK (BKV) nephropathy and has the potential for disease staging based on staining intensity and quantification of infected cells. This multicenter trial evaluated the reproducibility of BKV immunohistochemistry among 81 pathologists at 60 institutions. Participants stained tissue microarray slides and scored them for staining intensity and percentage of positive nuclei. Staining protocol details and evaluation scores were collected online. Slides were returned for centralized panel re-evaluation and kappa statistics were calculated. Individual assessment of staining intensity and percentage was more reproducible than combined scoring. Inter-institutional reproducibility was moderate for staining intensity (κ=0.49) and percentage (κ=0.42), fair for combined (κ=0.25), and best for simple positive/negative scoring (κ=0.63). Inter-observer reproducibility was substantial for intensity (κ=0.74), percentage (κ=0.66), and positive/negative (κ=0.67), and moderate for combined scoring (κ=0.43). Inter-laboratory reproducibility was fair for intensity (κ=0.37), percentage (κ=0.40), and combined (κ=0.24), but substantial for positive/negative scoring (κ=0.78). BKV RNA copies/cell correlated with staining intensity (r=0.56) and percentage (r=0.62). These results indicate that BKV immunohistochemistry is reproducible between observers but scoring should be simplified to a single-feature schema. Standardization of tissue processing and staining protocols would further improve inter-laboratory reproducibility.</p></div></front></TEI><affiliations><list><country><li>Autriche</li><li>Canada</li><li>États-Unis</li></country><region><li>Massachusetts</li><li>Pennsylvanie</li><li>Vienne (Autriche)</li></region><settlement><li>Boston</li><li>Pittsburgh</li><li>Vienne (Autriche)</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="Canada"><noRegion><name sortKey="Adam, Benjamin" sort="Adam, Benjamin" uniqKey="Adam B" first="Benjamin" last="Adam">Benjamin Adam</name></noRegion><name sortKey="Chan, Samantha" sort="Chan, Samantha" uniqKey="Chan S" first="Samantha" last="Chan">Samantha Chan</name><name sortKey="Chan, Samantha" sort="Chan, Samantha" uniqKey="Chan S" first="Samantha" last="Chan">Samantha Chan</name><name sortKey="Mengel, Michael" sort="Mengel, Michael" uniqKey="Mengel M" first="Michael" last="Mengel">Michael Mengel</name><name sortKey="Reeve, Jeff" sort="Reeve, Jeff" uniqKey="Reeve J" first="Jeff" last="Reeve">Jeff Reeve</name><name sortKey="Reeve, Jeff" sort="Reeve, Jeff" uniqKey="Reeve J" first="Jeff" last="Reeve">Jeff Reeve</name></country><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Randhawa, Parmjeet" sort="Randhawa, Parmjeet" uniqKey="Randhawa P" first="Parmjeet" last="Randhawa">Parmjeet Randhawa</name></region><name sortKey="Colvin, Robert B" sort="Colvin, Robert B" uniqKey="Colvin R" first="Robert B." last="Colvin">Robert B. Colvin</name><name sortKey="Kushner, Yael B" sort="Kushner, Yael B" uniqKey="Kushner Y" first="Yael B." last="Kushner">Yael B. Kushner</name><name sortKey="Zeng, Gang" sort="Zeng, Gang" uniqKey="Zeng G" first="Gang" last="Zeng">Gang Zeng</name></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Regele, Heinz" sort="Regele, Heinz" uniqKey="Regele H" first="Heinz" last="Regele">Heinz Regele</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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