PittsburghV1, Main, Exploration, bibRecord, 001907

Protein kinase C delta modulates endothelial nitric oxide synthase after cardiac arrest

Identifieur interne : 001907 ( Main/Exploration ); précédent : 001906; suivant : 001908

Protein kinase C delta modulates endothelial nitric oxide synthase after cardiac arrest

Auteurs : Hung Wen Lin [États-Unis] ; Victoria L. Gresia [États-Unis] ; Holly M. Stradecki [États-Unis] ; Aleksey Alekseyenko [États-Unis] ; Cameron Dezfulian [États-Unis] ; Jake T. Neumann [États-Unis] ; Kunjan R. Dave [États-Unis] ; Miguel A. Perez-Pinzon [États-Unis]

Source :

Journal of Cerebral Blood Flow & Metabolism [ 0271-678X ] ; 2014.

RBID : PMC:3982078

Descripteurs français

KwdFr :
- Animaux, Antienzymes (pharmacologie), Arginine (pharmacologie), Arrêt cardiaque (enzymologie), Arrêt cardiaque (physiopathologie), Circulation cérébrovasculaire (), Circulation cérébrovasculaire (physiologie), Cortex cérébral (), Cortex cérébral (enzymologie), Donneur d'oxyde nitrique (pharmacologie), Fluxmétrie laser Doppler, Microscopie confocale, Mâle, Nitric oxide synthase type III (antagonistes et inhibiteurs), Nitric oxide synthase type III (métabolisme), Nitrites (sang), Oligopeptides (pharmacologie), Protein kinase C-delta (antagonistes et inhibiteurs), Rat Sprague-Dawley, Rats, Spécificité du substrat, Vitesse du flux sanguin (), Vitesse du flux sanguin (physiologie).
MESH :
- antagonistes et inhibiteurs : Nitric oxide synthase type III, Protein kinase C-delta.
- enzymologie : Arrêt cardiaque, Cortex cérébral.
- métabolisme : Nitric oxide synthase type III.
- pharmacologie : Antienzymes, Arginine, Donneur d'oxyde nitrique, Oligopeptides.
- physiologie : Circulation cérébrovasculaire, Vitesse du flux sanguin.
- physiopathologie : Arrêt cardiaque.
- sang : Nitrites.
- Animaux, Circulation cérébrovasculaire, Cortex cérébral, Fluxmétrie laser Doppler, Microscopie confocale, Mâle, Rat Sprague-Dawley, Rats, Spécificité du substrat, Vitesse du flux sanguin.

English descriptors

KwdEn :
- Animals, Arginine (pharmacology), Blood Flow Velocity (drug effects), Blood Flow Velocity (physiology), Cerebral Cortex (blood supply), Cerebral Cortex (drug effects), Cerebral Cortex (enzymology), Cerebrovascular Circulation (drug effects), Cerebrovascular Circulation (physiology), Enzyme Inhibitors (pharmacology), Heart Arrest (enzymology), Heart Arrest (physiopathology), Laser-Doppler Flowmetry, Male, Microscopy, Confocal, Nitric Oxide Donors (pharmacology), Nitric Oxide Synthase Type III (antagonists & inhibitors), Nitric Oxide Synthase Type III (metabolism), Nitrites (blood), Oligopeptides (pharmacology), Protein Kinase C-delta (antagonists & inhibitors), Rats, Rats, Sprague-Dawley, Substrate Specificity.
MESH :
- chemical , antagonists & inhibitors : Nitric Oxide Synthase Type III, Protein Kinase C-delta.
- chemical , blood : Nitrites.
- chemical , metabolism : Nitric Oxide Synthase Type III.
- chemical , pharmacology : Arginine, Enzyme Inhibitors, Nitric Oxide Donors, Oligopeptides.
- blood supply : Cerebral Cortex.
- drug effects : Blood Flow Velocity, Cerebral Cortex, Cerebrovascular Circulation.
- enzymology : Cerebral Cortex, Heart Arrest.
- physiology : Blood Flow Velocity, Cerebrovascular Circulation.
- physiopathology : Heart Arrest.
- Animals, Laser-Doppler Flowmetry, Male, Microscopy, Confocal, Rats, Rats, Sprague-Dawley, Substrate Specificity.

Abstract

We previously showed that inhibition of protein kinase C delta (PKCδ) improves brain perfusion 24 hours after asphyxial cardiac arrest (ACA) and confers neuroprotection in the cortex and CA1 region of the hippocampus 7 days after arrest. Therefore, in this study, we investigate the mechanism of action of PKCδ-mediated hypoperfusion after ACA in the rat by using the two-photon laser scanning microscopy (TPLSM) to observe cortical cerebral blood flow (CBF) and laser Doppler flowmetry (LDF) detecting regional CBF in the presence/absence of δV1-1 (specific PKCδ inhibitor), nitric oxide synthase (NOS) substrate (L-arginine, L-arg) and inhibitor (N^ω-Nitro-L-arginine, NLA), and nitric oxide (NO) donor (sodium nitroprusside, SNP). There was an increase in regional LDF and local (TPLSM) CBF in the presence of δV1-1+L-arg, but only an increase in regional CBF under δV1-1+SNP treatments. Systemic blood nitrite levels were measured 15 minutes and 24 hours after ACA. Nitrite levels were enhanced by pretreatment with δV1-1 30 minutes before ACA possibly attributable to enhanced endothelial NOS protein levels. Our results suggest that PKCδ can modulate NO machinery in cerebral vasculature. Protein kinase C delta can depress endothelial NOS blunting CBF resulting in hypoperfusion, but can be reversed with δV1-1 improving brain perfusion, thus providing subsequent neuroprotection after ACA.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982078

DOI: 10.1038/jcbfm.2013.232
PubMed: 24447953
PubMed Central: 3982078

Affiliations:

États-Unis

Le document en format XML

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<term>Arginine (pharmacology)</term>
<term>Blood Flow Velocity (drug effects)</term>
<term>Blood Flow Velocity (physiology)</term>
<term>Cerebral Cortex (blood supply)</term>
<term>Cerebral Cortex (drug effects)</term>
<term>Cerebral Cortex (enzymology)</term>
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<term>Oligopeptides</term>
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<term>Arginine</term>
<term>Donneur d'oxyde nitrique</term>
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<term>Vitesse du flux sanguin</term>
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<term>Cerebrovascular Circulation</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Arrêt cardiaque</term>
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<term>Laser-Doppler Flowmetry</term>
<term>Male</term>
<term>Microscopy, Confocal</term>
<term>Rats</term>
<term>Rats, Sprague-Dawley</term>
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<front><div type="abstract" xml:lang="en"><p>We previously showed that inhibition of protein kinase C delta (PKC<italic>δ</italic>
) improves brain perfusion 24 hours after asphyxial cardiac arrest (ACA) and confers neuroprotection in the cortex and CA1 region of the hippocampus 7 days after arrest. Therefore, in this study, we investigate the mechanism of action of PKC<italic>δ</italic>
-mediated hypoperfusion after ACA in the rat by using the two-photon laser scanning microscopy (TPLSM) to observe cortical cerebral blood flow (CBF) and laser Doppler flowmetry (LDF) detecting regional CBF in the presence/absence of <italic>δ</italic>
V1-1 (specific PKC<italic>δ</italic>
 inhibitor), nitric oxide synthase (NOS) substrate (<sc>L</sc>
-arginine, <sc>L</sc>
-arg) and inhibitor (<italic>N</italic>
<sup><italic>ω</italic>
</sup>
-Nitro-<sc>L</sc>
-arginine, NLA), and nitric oxide (NO) donor (sodium nitroprusside, SNP). There was an increase in regional LDF and local (TPLSM) CBF in the presence of <italic>δ</italic>
V1-1+<sc>L</sc>
-arg, but only an increase in regional CBF under <italic>δ</italic>
V1-1+SNP treatments. Systemic blood nitrite levels were measured 15 minutes and 24 hours after ACA. Nitrite levels were enhanced by pretreatment with <italic>δ</italic>
V1-1 30 minutes before ACA possibly attributable to enhanced endothelial NOS protein levels. Our results suggest that PKC<italic>δ</italic>
 can modulate NO machinery in cerebral vasculature. Protein kinase C delta can depress endothelial NOS blunting CBF resulting in hypoperfusion, but can be reversed with <italic>δ</italic>
V1-1 improving brain perfusion, thus providing subsequent neuroprotection after ACA.</p>
</div>
</front>
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<name sortKey="Dave, Kunjan R" sort="Dave, Kunjan R" uniqKey="Dave K" first="Kunjan R" last="Dave">Kunjan R. Dave</name>
<name sortKey="Dezfulian, Cameron" sort="Dezfulian, Cameron" uniqKey="Dezfulian C" first="Cameron" last="Dezfulian">Cameron Dezfulian</name>
<name sortKey="Gresia, Victoria L" sort="Gresia, Victoria L" uniqKey="Gresia V" first="Victoria L" last="Gresia">Victoria L. Gresia</name>
<name sortKey="Neumann, Jake T" sort="Neumann, Jake T" uniqKey="Neumann J" first="Jake T" last="Neumann">Jake T. Neumann</name>
<name sortKey="Perez Pinzon, Miguel A" sort="Perez Pinzon, Miguel A" uniqKey="Perez Pinzon M" first="Miguel A" last="Perez-Pinzon">Miguel A. Perez-Pinzon</name>
<name sortKey="Stradecki, Holly M" sort="Stradecki, Holly M" uniqKey="Stradecki H" first="Holly M" last="Stradecki">Holly M. Stradecki</name>
</country>
</tree>
</affiliations>
</record>

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HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001907 | SxmlIndent | more

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{{Explor lien
   |wiki=    Wicri/Amérique
   |area=    PittsburghV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3982078
   |texte=   Protein kinase C delta modulates endothelial nitric oxide synthase after cardiac arrest
}}

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       | NlmPubMed2Wicri -a PittsburghV1

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Serveur d'exploration sur Pittsburgh

Protein kinase C delta modulates endothelial nitric oxide synthase after cardiac arrest

Protein kinase C delta modulates endothelial nitric oxide synthase after cardiac arrest

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Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur Pittsburgh
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