Composition of human islet cell preparations for transplantation
Identifieur interne : 002585 ( Istex/Corpus ); précédent : 002584; suivant : 002586Composition of human islet cell preparations for transplantation
Auteurs : C. E. Sever ; A. J. Demetris ; J. Zeng ; P. Carroll ; A. Tzakis ; J. J. Fung ; T. E. Starzl ; C. RicordiSource :
- Acta Diabetologica [ 0940-5429 ] ; 1992-09-01.
English descriptors
- KwdEn :
- Acinar, Acinar cells, Ceils, Cell pellets, Cell suspension, Cell suspensions, Cell type, Cell types, Cellular composition, Clinical trials, Connective tissue, Dendritic, Dendritic cells, Ductal, Ductal elements, Endothelial cells, Graft, Graft survival, Granule, Haematolymphoid components, Haematopoietic origin, Human islet cell preparations, Human islets, Immunoperoxidase, Immunoperoxidase stains, Insulin, Islet, Islet ceils, Islet cell, Islet cell clusters, Islet cell content, Islet cell preparations, Islet cell suspensions, Islet cells, Macrophage, Myoepithelial cells, Normal pancreas, Pancreas, Pancreatic, Pancreatic islet transplantation, Positive staining, Primary antibody, Rejection response, Significant decrease, Soft tissue, Staining intensity, Transplant proc, Transplantation.
- Teeft :
- Acinar, Acinar cells, Ceils, Cell pellets, Cell suspension, Cell suspensions, Cell type, Cell types, Cellular composition, Clinical trials, Connective tissue, Dendritic, Dendritic cells, Ductal, Ductal elements, Endothelial cells, Graft, Graft survival, Granule, Haematolymphoid components, Haematopoietic origin, Human islet cell preparations, Human islets, Immunoperoxidase, Immunoperoxidase stains, Insulin, Islet, Islet ceils, Islet cell, Islet cell clusters, Islet cell content, Islet cell preparations, Islet cell suspensions, Islet cells, Macrophage, Myoepithelial cells, Normal pancreas, Pancreas, Pancreatic, Pancreatic islet transplantation, Positive staining, Primary antibody, Rejection response, Significant decrease, Soft tissue, Staining intensity, Transplant proc, Transplantation.
Abstract
Abstract: To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30–80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10–20%. After 1 week in culture the islet cell content of less highly purified isolates (30–40% islets) dropped dramatically to 5%. The highly purified isolates (70–80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment.
Url:
DOI: 10.1007/BF00779005
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30–80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10–20%. After 1 week in culture the islet cell content of less highly purified isolates (30–40% islets) dropped dramatically to 5%. The highly purified isolates (70–80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment.</div>
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<abstract xml:lang="en"><p>Abstract: To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30–80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10–20%. After 1 week in culture the islet cell content of less highly purified isolates (30–40% islets) dropped dramatically to 5%. The highly purified isolates (70–80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment.</p>
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<abstract lang="en">Abstract: To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30–80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10–20%. After 1 week in culture the islet cell content of less highly purified isolates (30–40% islets) dropped dramatically to 5%. The highly purified isolates (70–80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment.</abstract>
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