Interleukin‐12 Gene Therapy Prevents Establishment of SCC VII Squamous Cell Carcinomas, Inhibits Tumor Growth, and Elicits Long‐term Antitumor Immunity in Syngeneic C3H Mice
Identifieur interne : 002261 ( Istex/Corpus ); précédent : 002260; suivant : 002262Interleukin‐12 Gene Therapy Prevents Establishment of SCC VII Squamous Cell Carcinomas, Inhibits Tumor Growth, and Elicits Long‐term Antitumor Immunity in Syngeneic C3H Mice
Auteurs : Jeffrey N. Myers ; Amy Mank-Seymour ; Laurence Zitvogel ; Walter Storkus ; Martha Clarke ; Candace S. Johnson ; Hideaki Tahara ; Michael T. LotzeSource :
- The Laryngoscope [ 0023-852X ] ; 1998-02.
English descriptors
- KwdEn :
- American laryngological, Antisense primers, Antitumor, Antitumor efficacy, Antitumor immunity, Arch otolaryngol head neck surg, Base pairs, C3whej, C3whej mice, Carcinoma, Cell carcinoma, Cell line, Cell number, Cesium source, Chain reaction, Cytokine, Different tumors, Distinct genes, Dose range, Ethidium bromide, February, Fragment size, Gene, Gene therapy, Gene transfection, Genes encoding, Head neck, Heterodimer antibody, Hierarchical immunosuppression, High levels, Immunosorbent assay, Immunostimulatory, Immunostimulatory agent, Immunostimulatory agents, Inoculated, Inoculated subcutaneously, Laryngoscope, Liquid nitrogen, Locoregional immunosuppression, Lymphoblast proliferation assay, Lymphocyte, Monoclonal antibody, Monoclonal antimouse, Murine, Murine tumors, Myers, Naive mice, Natural killer cell, Neck cancer, Otolaryngol head neck surg, Otological society, Palpable tumor, Palpable tumors, Peripheral blood, Peritumoral injections, Scchn, Size differences, Squamous, Squamous cell carcinoma, Squamous cell carcinomas, Subcutaneously, Subunit, Subunit primers, Supernatant, Tissue culture, Transfected, Tumor, Tumor cells, Tumor establishment, Tumor establishment model, Tumor growth, Tumor models, Tumor size, Tumors transfected, Untreated groups, Vivneo, Vivneo tumor cells.
- Teeft :
- American laryngological, Antisense primers, Antitumor, Antitumor efficacy, Antitumor immunity, Arch otolaryngol head neck surg, Base pairs, C3whej, C3whej mice, Carcinoma, Cell carcinoma, Cell line, Cell number, Cesium source, Chain reaction, Cytokine, Different tumors, Distinct genes, Dose range, Ethidium bromide, February, Fragment size, Gene, Gene therapy, Gene transfection, Genes encoding, Head neck, Heterodimer antibody, Hierarchical immunosuppression, High levels, Immunosorbent assay, Immunostimulatory, Immunostimulatory agent, Immunostimulatory agents, Inoculated, Inoculated subcutaneously, Laryngoscope, Liquid nitrogen, Locoregional immunosuppression, Lymphoblast proliferation assay, Lymphocyte, Monoclonal antibody, Monoclonal antimouse, Murine, Murine tumors, Myers, Naive mice, Natural killer cell, Neck cancer, Otolaryngol head neck surg, Otological society, Palpable tumor, Palpable tumors, Peripheral blood, Peritumoral injections, Scchn, Size differences, Squamous, Squamous cell carcinoma, Squamous cell carcinomas, Subcutaneously, Subunit, Subunit primers, Supernatant, Tissue culture, Transfected, Tumor, Tumor cells, Tumor establishment, Tumor establishment model, Tumor growth, Tumor models, Tumor size, Tumors transfected, Untreated groups, Vivneo, Vivneo tumor cells.
Abstract
Interleukin‐12 (IL‐12) is an immunostimulatory agent with very promising antitumor activity. Using a retroviral expression vector, the authors have successfully transduced the genes encoding the two subunits of murine IL‐12 to the squamous cell carcinoma cell line, SCC VII. Once IL‐12 gene transcription and protein production were successfully verified, IL‐12 expression was found to inhibit the establishment of SCC VII tumors in syngeneic C3H/HeJ mice inoculated with 1 × 106 SCC VII/IL‐12 viable tumor cells. Mice immunized in this manner and rechallenged with parent SCC VII were capable of rejecting tumor up to 40% of the time. Treatment of established SCC VII tumors with irradiated IL‐12‐producing tumors cells led to significant tumor regression in a high percentage of animals.
Url:
DOI: 10.1097/00005537-199802000-00019
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Interleukin‐12 (IL‐12) is an immunostimulatory agent with very promising antitumor activity. Using a retroviral expression vector, the authors have successfully transduced the genes encoding the two subunits of murine IL‐12 to the squamous cell carcinoma cell line, SCC VII. Once IL‐12 gene transcription and protein production were successfully verified, IL‐12 expression was found to inhibit the establishment of SCC VII tumors in syngeneic C3H/HeJ mice inoculated with 1 × 106 SCC VII/IL‐12 viable tumor cells. Mice immunized in this manner and rechallenged with parent SCC VII were capable of rejecting tumor up to 40% of the time. Treatment of established SCC VII tumors with irradiated IL‐12‐producing tumors cells led to significant tumor regression in a high percentage of animals.</div>
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