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Interleukin‐12 Gene Therapy Prevents Establishment of SCC VII Squamous Cell Carcinomas, Inhibits Tumor Growth, and Elicits Long‐term Antitumor Immunity in Syngeneic C3H Mice

Identifieur interne : 002261 ( Istex/Corpus ); précédent : 002260; suivant : 002262

Interleukin‐12 Gene Therapy Prevents Establishment of SCC VII Squamous Cell Carcinomas, Inhibits Tumor Growth, and Elicits Long‐term Antitumor Immunity in Syngeneic C3H Mice

Auteurs : Jeffrey N. Myers ; Amy Mank-Seymour ; Laurence Zitvogel ; Walter Storkus ; Martha Clarke ; Candace S. Johnson ; Hideaki Tahara ; Michael T. Lotze

Source :

RBID : ISTEX:93DF7AED49D30F00E8AC42240F0B5A7F9E2B9C4E

English descriptors

Abstract

Interleukin‐12 (IL‐12) is an immunostimulatory agent with very promising antitumor activity. Using a retroviral expression vector, the authors have successfully transduced the genes encoding the two subunits of murine IL‐12 to the squamous cell carcinoma cell line, SCC VII. Once IL‐12 gene transcription and protein production were successfully verified, IL‐12 expression was found to inhibit the establishment of SCC VII tumors in syngeneic C3H/HeJ mice inoculated with 1 × 106 SCC VII/IL‐12 viable tumor cells. Mice immunized in this manner and rechallenged with parent SCC VII were capable of rejecting tumor up to 40% of the time. Treatment of established SCC VII tumors with irradiated IL‐12‐producing tumors cells led to significant tumor regression in a high percentage of animals.

Url:
DOI: 10.1097/00005537-199802000-00019

Links to Exploration step

ISTEX:93DF7AED49D30F00E8AC42240F0B5A7F9E2B9C4E

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<div type="abstract" xml:lang="en">Interleukin‐12 (IL‐12) is an immunostimulatory agent with very promising antitumor activity. Using a retroviral expression vector, the authors have successfully transduced the genes encoding the two subunits of murine IL‐12 to the squamous cell carcinoma cell line, SCC VII. Once IL‐12 gene transcription and protein production were successfully verified, IL‐12 expression was found to inhibit the establishment of SCC VII tumors in syngeneic C3H/HeJ mice inoculated with 1 × 106 SCC VII/IL‐12 viable tumor cells. Mice immunized in this manner and rechallenged with parent SCC VII were capable of rejecting tumor up to 40% of the time. Treatment of established SCC VII tumors with irradiated IL‐12‐producing tumors cells led to significant tumor regression in a high percentage of animals.</div>
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