Effects of Growth Conditions on Carbon Utilization and Organic By‐Product Formation in B. subtilis
Identifieur interne : 001E94 ( Istex/Corpus ); précédent : 001E93; suivant : 001E95Effects of Growth Conditions on Carbon Utilization and Organic By‐Product Formation in B. subtilis
Auteurs : J. Snay ; J. W. Jeong ; M. M. AtaaiSource :
- Biotechnology Progress [ 8756-7938 ] ; 1989-06.
English descriptors
- KwdEn :
- Academic press, Acetic, Acetic acid, Acetoin, Acid production, Bacillus, Biomass formation, Biosynthetic pathways, Blotechnology progress, Carbon substrate, Carbon utilization, Cell cultures, Cell density, Cell growth, Cell mass, Cell yields, Cellular biosynthesis, Chemostat, Chemostat cultures, Complete utilization, Cycle activity, Dilution, Dilution rate, Dilution rates, Efficient utilization, Extracellular side products, Formic, Formic acid, Formic acids, Glucose, Glucose carbon, Glucose catabolism, Glucose conc, Glucose concentration, Glucose feed concentration, Glucose feed levels, Glucose metabolism, Glucose uptake rate, Glucosell medium, Growth conditions, Growth rate, Growth rates, Hplc system, Incomplete utilization, Lactic, Lactic acid, Lactic acid production, Medium components, Metabolic, Metabolic shift, Metabolism, Nadh dehydrogenase, Organic acids, Other components, Pathway, Product formation, Proportional increase, Protein production, Residence time, Residual, Residual glucose, Side product concentrations, Side product formation, Side products, Specific growth rate, Steady state, Step change, Substrate concentration, Subtilis, Transient changes.
- Teeft :
- Academic press, Acetic, Acetic acid, Acetoin, Acid production, Bacillus, Biomass formation, Biosynthetic pathways, Blotechnology progress, Carbon substrate, Carbon utilization, Cell cultures, Cell density, Cell growth, Cell mass, Cell yields, Cellular biosynthesis, Chemostat, Chemostat cultures, Complete utilization, Cycle activity, Dilution, Dilution rate, Dilution rates, Efficient utilization, Extracellular side products, Formic, Formic acid, Formic acids, Glucose, Glucose carbon, Glucose catabolism, Glucose conc, Glucose concentration, Glucose feed concentration, Glucose feed levels, Glucose metabolism, Glucose uptake rate, Glucosell medium, Growth conditions, Growth rate, Growth rates, Hplc system, Incomplete utilization, Lactic, Lactic acid, Lactic acid production, Medium components, Metabolic, Metabolic shift, Metabolism, Nadh dehydrogenase, Organic acids, Other components, Pathway, Product formation, Proportional increase, Protein production, Residence time, Residual, Residual glucose, Side product concentrations, Side product formation, Side products, Specific growth rate, Steady state, Step change, Substrate concentration, Subtilis, Transient changes.
Abstract
The effects of growth rate and substrate concentration on cellular metabolism of glucose was studied with cultures of B. subtilis 168. Application of chemostat culture techniques allowed for the observation of each growth condition independent of the other. The relative flux of carbon through biosynthetic and non‐biosynthetic pathways was determined by monitoring cell density and distribution of extracellular side products in active cultures. A metabolic shift of glucose utilization pathways was observed in cell cultures grown at dilution rates greater than 0.36 hr−1 or with glucose feed levels exceeding 10 gll. The predominant side products detected by HPLC analysis were acetic acid, formic acid, lactic acid, and acetoin. The patterns of side product formation were analyzed in relation to the applied growth conditions.
Url:
DOI: 10.1002/btpr.5420050207
Links to Exploration step
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<front><div type="abstract" xml:lang="en">The effects of growth rate and substrate concentration on cellular metabolism of glucose was studied with cultures of B. subtilis 168. Application of chemostat culture techniques allowed for the observation of each growth condition independent of the other. The relative flux of carbon through biosynthetic and non‐biosynthetic pathways was determined by monitoring cell density and distribution of extracellular side products in active cultures. A metabolic shift of glucose utilization pathways was observed in cell cultures grown at dilution rates greater than 0.36 hr−1 or with glucose feed levels exceeding 10 gll. The predominant side products detected by HPLC analysis were acetic acid, formic acid, lactic acid, and acetoin. The patterns of side product formation were analyzed in relation to the applied growth conditions.</div>
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<abstract>The effects of growth rate and substrate concentration on cellular metabolism of glucose was studied with cultures of B. subtilis 168. Application of chemostat culture techniques allowed for the observation of each growth condition independent of the other. The relative flux of carbon through biosynthetic and non‐biosynthetic pathways was determined by monitoring cell density and distribution of extracellular side products in active cultures. A metabolic shift of glucose utilization pathways was observed in cell cultures grown at dilution rates greater than 0.36 hr−1 or with glucose feed levels exceeding 10 gll. The predominant side products detected by HPLC analysis were acetic acid, formic acid, lactic acid, and acetoin. The patterns of side product formation were analyzed in relation to the applied growth conditions.</abstract>
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