Evaluation of alpha-synuclein immunohistochemical methods for the detection of Lewy-type synucleinopathy in gastrointestinal biopsies.
Identifieur interne : 000251 ( PubMed/Curation ); précédent : 000250; suivant : 000252Evaluation of alpha-synuclein immunohistochemical methods for the detection of Lewy-type synucleinopathy in gastrointestinal biopsies.
Auteurs : Anne-Gaëlle Corbillé [France] ; Franck Letournel [France] ; Jeffrey H. Kordower [États-Unis] ; John Lee [États-Unis] ; Elisheva Shanes [États-Unis] ; Michel Neunlist [France] ; David G. Munoz [Canada] ; Pascal Derkinderen [France] ; Thomas G. Beach [États-Unis]Source :
- Acta neuropathologica communications [ 2051-5960 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : alpha-Synuclein.
- metabolism : Gastrointestinal Tract, Parkinson Disease.
- pathology : Gastrointestinal Tract, Lewy Bodies, Parkinson Disease.
- Aged, Biopsy, Female, Humans, Male, Middle Aged.
Abstract
The observation showing that Lewy type synucleinopathy (LTS), the pathological hallmark of Parkinson's disease (PD), is found in the gut of almost all PD subjects led to a substantial amount of research to develop a diagnostic procedure in living patients based on endoscopically obtained gastrointestinal biopsies. However, the existing studies have provided conflicting results regarding the sensitivity and specificity of gastrointestinal biopsies for the detection of LTS. We therefore undertook a multi-center staining and blinded judging of a common set of slides from colonic biopsies to determine the optimal protocol for the detection of LTS. Four different immunohistochemical methods, developed in four separate expert laboratories, were evaluated for their sensitivity and specificity to detect enteric LTS. Test sets of formalin-fixed, paraffin-embedded sections from biopsies of 9 PD subjects and 3 controls were stained with the 4 methods and graded by 4 different observers. Four types of staining morphology (granular staining in the lamina propria, perivascular/vascular wall staining in the submucosa, lacy-granular pattern in the submucosa and epithelial cell nuclear staining) were variably observed in the slides stained by the 4 methods. Positive alpha-synuclein staining was observed by all 5 judges in most of the slides from control cases, regardless of the staining methods that were used. Moreover, none of the tested method or staining pattern had a specificity and sensitivity more than 80 % regarding to PD. Overall, our study suggest that the tested methods are not adequate for the prediction of PD using gastrointestinal biopsies. Future studies are warranted to test new immunostaining methods.
DOI: 10.1186/s40478-016-0305-8
PubMed: 27044604
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Beach</name><affiliation wicri:level="1"><nlm:affiliation>Banner Sun Health Research Institute, Sun City, AZ, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Banner Sun Health Research Institute, Sun City, AZ</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27044604</idno><idno type="pmid">27044604</idno><idno type="doi">10.1186/s40478-016-0305-8</idno><idno type="wicri:Area/PubMed/Corpus">000251</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000251</idno><idno type="wicri:Area/PubMed/Curation">000251</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000251</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of alpha-synuclein immunohistochemical methods for the detection of Lewy-type synucleinopathy in gastrointestinal biopsies.</title><author><name sortKey="Corbille, Anne Gaelle" sort="Corbille, Anne Gaelle" uniqKey="Corbille A" first="Anne-Gaëlle" last="Corbillé">Anne-Gaëlle Corbillé</name><affiliation wicri:level="1"><nlm:affiliation>Inserm, U913, Nantes, F-44035, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44035</wicri:regionArea></affiliation></author><author><name sortKey="Letournel, Franck" sort="Letournel, Franck" uniqKey="Letournel F" first="Franck" last="Letournel">Franck Letournel</name><affiliation wicri:level="1"><nlm:affiliation>CHU Angers, Neurobiology and Neuropathology Laboratory, Angers, F-49033, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CHU Angers, Neurobiology and Neuropathology Laboratory, Angers, F-49033</wicri:regionArea></affiliation></author><author><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H" last="Kordower">Jeffrey H. Kordower</name><affiliation wicri:level="1"><nlm:affiliation>Department of Center for Brain Repair, Department of Pathology, Rush Medical College, Chicago, IL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Center for Brain Repair, Department of Pathology, Rush Medical College, Chicago, IL</wicri:regionArea></affiliation></author><author><name sortKey="Lee, John" sort="Lee, John" uniqKey="Lee J" first="John" last="Lee">John Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, NorthShore Medical Group, Evanston, IL, 60201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, NorthShore Medical Group, Evanston, IL, 60201</wicri:regionArea></affiliation></author><author><name sortKey="Shanes, Elisheva" sort="Shanes, Elisheva" uniqKey="Shanes E" first="Elisheva" last="Shanes">Elisheva Shanes</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, NorthShore Medical Group, Evanston, IL, 60201, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, NorthShore Medical Group, Evanston, IL, 60201</wicri:regionArea></affiliation></author><author><name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name><affiliation wicri:level="1"><nlm:affiliation>Inserm, U913, Nantes, F-44035, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44035</wicri:regionArea></affiliation></author><author><name sortKey="Munoz, David G" sort="Munoz, David G" uniqKey="Munoz D" first="David G" last="Munoz">David G. Munoz</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory Medicine, St. Michael's Hospital, University of Toronto & Li Ka Shing Knowledge Institute, Toronto, ON, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Laboratory Medicine, St. Michael's Hospital, University of Toronto & Li Ka Shing Knowledge Institute, Toronto, ON</wicri:regionArea></affiliation></author><author><name sortKey="Derkinderen, Pascal" sort="Derkinderen, Pascal" uniqKey="Derkinderen P" first="Pascal" last="Derkinderen">Pascal Derkinderen</name><affiliation wicri:level="1"><nlm:affiliation>Inserm, U913, Nantes, F-44035, France. derkinderenp@yahoo.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U913, Nantes, F-44035</wicri:regionArea></affiliation></author><author><name sortKey="Beach, Thomas G" sort="Beach, Thomas G" uniqKey="Beach T" first="Thomas G" last="Beach">Thomas G. Beach</name><affiliation wicri:level="1"><nlm:affiliation>Banner Sun Health Research Institute, Sun City, AZ, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Banner Sun Health Research Institute, Sun City, AZ</wicri:regionArea></affiliation></author></analytic><series><title level="j">Acta neuropathologica communications</title><idno type="eISSN">2051-5960</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term><term>Biopsy</term><term>Female</term><term>Gastrointestinal Tract (metabolism)</term><term>Gastrointestinal Tract (pathology)</term><term>Humans</term><term>Lewy Bodies (pathology)</term><term>Male</term><term>Middle Aged</term><term>Parkinson Disease (metabolism)</term><term>Parkinson Disease (pathology)</term><term>alpha-Synuclein (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>alpha-Synuclein</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gastrointestinal Tract</term><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Gastrointestinal Tract</term><term>Lewy Bodies</term><term>Parkinson Disease</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Biopsy</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The observation showing that Lewy type synucleinopathy (LTS), the pathological hallmark of Parkinson's disease (PD), is found in the gut of almost all PD subjects led to a substantial amount of research to develop a diagnostic procedure in living patients based on endoscopically obtained gastrointestinal biopsies. However, the existing studies have provided conflicting results regarding the sensitivity and specificity of gastrointestinal biopsies for the detection of LTS. We therefore undertook a multi-center staining and blinded judging of a common set of slides from colonic biopsies to determine the optimal protocol for the detection of LTS. Four different immunohistochemical methods, developed in four separate expert laboratories, were evaluated for their sensitivity and specificity to detect enteric LTS. Test sets of formalin-fixed, paraffin-embedded sections from biopsies of 9 PD subjects and 3 controls were stained with the 4 methods and graded by 4 different observers. Four types of staining morphology (granular staining in the lamina propria, perivascular/vascular wall staining in the submucosa, lacy-granular pattern in the submucosa and epithelial cell nuclear staining) were variably observed in the slides stained by the 4 methods. Positive alpha-synuclein staining was observed by all 5 judges in most of the slides from control cases, regardless of the staining methods that were used. Moreover, none of the tested method or staining pattern had a specificity and sensitivity more than 80 % regarding to PD. Overall, our study suggest that the tested methods are not adequate for the prediction of PD using gastrointestinal biopsies. Future studies are warranted to test new immunostaining methods.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27044604</PMID><DateCreated><Year>2016</Year><Month>04</Month><Day>05</Day></DateCreated><DateCompleted><Year>2016</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2051-5960</ISSN><JournalIssue CitedMedium="Internet"><Volume>4</Volume><PubDate><Year>2016</Year><Month>Apr</Month><Day>04</Day></PubDate></JournalIssue><Title>Acta neuropathologica communications</Title><ISOAbbreviation>Acta Neuropathol Commun</ISOAbbreviation></Journal><ArticleTitle>Evaluation of alpha-synuclein immunohistochemical methods for the detection of Lewy-type synucleinopathy in gastrointestinal biopsies.</ArticleTitle><Pagination><MedlinePgn>35</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s40478-016-0305-8</ELocationID><Abstract><AbstractText>The observation showing that Lewy type synucleinopathy (LTS), the pathological hallmark of Parkinson's disease (PD), is found in the gut of almost all PD subjects led to a substantial amount of research to develop a diagnostic procedure in living patients based on endoscopically obtained gastrointestinal biopsies. However, the existing studies have provided conflicting results regarding the sensitivity and specificity of gastrointestinal biopsies for the detection of LTS. We therefore undertook a multi-center staining and blinded judging of a common set of slides from colonic biopsies to determine the optimal protocol for the detection of LTS. Four different immunohistochemical methods, developed in four separate expert laboratories, were evaluated for their sensitivity and specificity to detect enteric LTS. Test sets of formalin-fixed, paraffin-embedded sections from biopsies of 9 PD subjects and 3 controls were stained with the 4 methods and graded by 4 different observers. Four types of staining morphology (granular staining in the lamina propria, perivascular/vascular wall staining in the submucosa, lacy-granular pattern in the submucosa and epithelial cell nuclear staining) were variably observed in the slides stained by the 4 methods. Positive alpha-synuclein staining was observed by all 5 judges in most of the slides from control cases, regardless of the staining methods that were used. Moreover, none of the tested method or staining pattern had a specificity and sensitivity more than 80 % regarding to PD. Overall, our study suggest that the tested methods are not adequate for the prediction of PD using gastrointestinal biopsies. Future studies are warranted to test new immunostaining methods.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Corbillé</LastName><ForeName>Anne-Gaëlle</ForeName><Initials>AG</Initials><AffiliationInfo><Affiliation>Inserm, U913, Nantes, F-44035, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Nantes University, Nantes, F-44035, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Neurology, CHU Nantes, Nantes, F-44093, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CHU Angers, Neurobiology and Neuropathology Laboratory, Angers, F-49033, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université of Angers, UPRES EA3143, Angers, F-49033, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Letournel</LastName><ForeName>Franck</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>CHU Angers, Neurobiology and Neuropathology Laboratory, Angers, F-49033, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université of Angers, UPRES EA3143, Angers, F-49033, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kordower</LastName><ForeName>Jeffrey H</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Center for Brain Repair, Department of Pathology, Rush Medical College, Chicago, IL, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pathology, NorthShore Medical Group, Evanston, IL, 60201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shanes</LastName><ForeName>Elisheva</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Pathology, NorthShore Medical Group, Evanston, IL, 60201, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neunlist</LastName><ForeName>Michel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Inserm, U913, Nantes, F-44035, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Nantes University, Nantes, F-44035, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Munoz</LastName><ForeName>David G</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>Laboratory Medicine, St. Michael's Hospital, University of Toronto & Li Ka Shing Knowledge Institute, Toronto, ON, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Derkinderen</LastName><ForeName>Pascal</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Inserm, U913, Nantes, F-44035, France. derkinderenp@yahoo.fr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Nantes University, Nantes, F-44035, France. derkinderenp@yahoo.fr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Neurology, CHU Nantes, Nantes, F-44093, France. derkinderenp@yahoo.fr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Neurology, CHU Nantes, 44093, Nantes, France. derkinderenp@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Beach</LastName><ForeName>Thomas G</ForeName><Initials>TG</Initials><AffiliationInfo><Affiliation>Banner Sun Health Research Institute, Sun City, AZ, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Acta Neuropathol Commun</MedlineTA><NlmUniqueID>101610673</NlmUniqueID><ISSNLinking>2051-5960</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C497604">SNCA protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051844">alpha-Synuclein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Mov Disord. 2013 Feb;28(2):237-40</RefSource><PMID Version="1">23362176</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurogastroenterol Motil. 2010 Jan;22(1):e11-4</RefSource><PMID Version="1">19650774</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mov Disord. 2014 Apr;29(4):444-50</RefSource><PMID Version="1">24375496</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Neuropathol. 1988;76(3):217-221</RefSource><PMID Version="1">2850698</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mov Disord. 2014 Jul;29(8):1010-8</RefSource><PMID Version="1">24395122</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurology. 1987 Jul;37(7):1253-5</RefSource><PMID Version="1">3037441</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Gastroenterology. 1984 Oct;87(4):848-56</RefSource><PMID Version="1">6088351</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2010;5(9):e12728</RefSource><PMID Version="1">20856865</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 1982 Jun 3;297(5865):409-10</RefSource><PMID Version="1">7043279</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochem Biophys Res Commun. 2007 Jun 22;358(1):104-10</RefSource><PMID Version="1">17475220</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mov Disord. 2012 May;27(6):709-15</RefSource><PMID Version="1">21766334</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Front Psychiatry. 2010 Sep 03;1:128</RefSource><PMID Version="1">21423439</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mov Disord. 2016 Feb;31(2):241-9</RefSource><PMID Version="1">26686342</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurogastroenterol Motil. 2012 Apr;24(4):e202-5</RefSource><PMID Version="1">22292943</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurosci Lett. 2006 Mar 20;396(1):67-72</RefSource><PMID Version="1">16330147</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurosci Lett. 2002 Jun 21;326(1):5-8</RefSource><PMID Version="1">12052525</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 1997 Aug 28;388(6645):839-40</RefSource><PMID Version="1">9278044</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mov Disord. 2015 Apr;30(4):517-24</RefSource><PMID Version="1">25113060</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Neuropathol. 2014 Feb;127(2):235-41</RefSource><PMID Version="1">24240814</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Neuropathol. 2010 Jun;119(6):689-702</RefSource><PMID Version="1">20306269</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Cell Biol. 2002 Feb;4(2):160-4</RefSource><PMID Version="1">11813001</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Neurol. 2016 Feb;23(2):247-61</RefSource><PMID Version="1">26100920</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurosci Lett. 2013 Sep 13;551:62-4</RefSource><PMID Version="1">23880024</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neurology. 2015 Feb 10;84(6):609-16</RefSource><PMID Version="1">25589666</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Gut. 2008 Dec;57(12):1741-3</RefSource><PMID Version="1">19022934</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Lancet. 2002 Apr 13;359(9314):1291-300</RefSource><PMID Version="1">11965274</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Neuropathol. 2008 Sep;116(3):277-88</RefSource><PMID Version="1">18626651</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001706" MajorTopicYN="N">Biopsy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D041981" MajorTopicYN="N">Gastrointestinal 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UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4820972</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Alpha-synuclein</Keyword><Keyword MajorTopicYN="N">Biomarker</Keyword><Keyword MajorTopicYN="N">Gastrointestinal biopsies</Keyword><Keyword MajorTopicYN="N">Parkinson’s disease</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>03</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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