Panorama of ancient metazoan macromolecular complexes.
Identifieur interne : 000415 ( PubMed/Corpus ); précédent : 000414; suivant : 000416Panorama of ancient metazoan macromolecular complexes.
Auteurs : Cuihong Wan ; Blake Borgeson ; Sadhna Phanse ; Fan Tu ; Kevin Drew ; Greg Clark ; Xuejian Xiong ; Olga Kagan ; Julian Kwan ; Alexandr Bezginov ; Kyle Chessman ; Swati Pal ; Graham Cromar ; Ophelia Papoulas ; Zuyao Ni ; Daniel R. Boutz ; Snejana Stoilova ; Pierre C. Havugimana ; Xinghua Guo ; Ramy H. Malty ; Mihail Sarov ; Jack Greenblatt ; Mohan Babu ; W Brent Derry ; Elisabeth R. Tillier ; John B. Wallingford ; John Parkinson ; Edward M. Marcotte ; Andrew EmiliSource :
- Nature [ 1476-4687 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Multiprotein Complexes.
- chemical , metabolism : Multiprotein Complexes.
- Animals, Datasets as Topic, Evolution, Molecular, Humans, Protein Interaction Mapping, Protein Interaction Maps, Reproducibility of Results, Systems Biology, Tandem Mass Spectrometry.
Abstract
Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.
DOI: 10.1038/nature14877
PubMed: 26344197
Links to Exploration step
pubmed:26344197Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Panorama of ancient metazoan macromolecular complexes.</title><author><name sortKey="Wan, Cuihong" sort="Wan, Cuihong" uniqKey="Wan C" first="Cuihong" last="Wan">Cuihong Wan</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Borgeson, Blake" sort="Borgeson, Blake" uniqKey="Borgeson B" first="Blake" last="Borgeson">Blake Borgeson</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Phanse, Sadhna" sort="Phanse, Sadhna" uniqKey="Phanse S" first="Sadhna" last="Phanse">Sadhna Phanse</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Tu, Fan" sort="Tu, Fan" uniqKey="Tu F" first="Fan" last="Tu">Fan Tu</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Drew, Kevin" sort="Drew, Kevin" uniqKey="Drew K" first="Kevin" last="Drew">Kevin Drew</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Clark, Greg" sort="Clark, Greg" uniqKey="Clark G" first="Greg" last="Clark">Greg Clark</name><affiliation><nlm:affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Xiong, Xuejian" sort="Xiong, Xuejian" uniqKey="Xiong X" first="Xuejian" last="Xiong">Xuejian Xiong</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Kagan, Olga" sort="Kagan, Olga" uniqKey="Kagan O" first="Olga" last="Kagan">Olga Kagan</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Kwan, Julian" sort="Kwan, Julian" uniqKey="Kwan J" first="Julian" last="Kwan">Julian Kwan</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Bezginov, Alexandr" sort="Bezginov, Alexandr" uniqKey="Bezginov A" first="Alexandr" last="Bezginov">Alexandr Bezginov</name><affiliation><nlm:affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Chessman, Kyle" sort="Chessman, Kyle" uniqKey="Chessman K" first="Kyle" last="Chessman">Kyle Chessman</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Pal, Swati" sort="Pal, Swati" uniqKey="Pal S" first="Swati" last="Pal">Swati Pal</name><affiliation><nlm:affiliation>Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Cromar, Graham" sort="Cromar, Graham" uniqKey="Cromar G" first="Graham" last="Cromar">Graham Cromar</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Papoulas, Ophelia" sort="Papoulas, Ophelia" uniqKey="Papoulas O" first="Ophelia" last="Papoulas">Ophelia Papoulas</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ni, Zuyao" sort="Ni, Zuyao" uniqKey="Ni Z" first="Zuyao" last="Ni">Zuyao Ni</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Boutz, Daniel R" sort="Boutz, Daniel R" uniqKey="Boutz D" first="Daniel R" last="Boutz">Daniel R. Boutz</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Stoilova, Snejana" sort="Stoilova, Snejana" uniqKey="Stoilova S" first="Snejana" last="Stoilova">Snejana Stoilova</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Havugimana, Pierre C" sort="Havugimana, Pierre C" uniqKey="Havugimana P" first="Pierre C" last="Havugimana">Pierre C. Havugimana</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Xinghua" sort="Guo, Xinghua" uniqKey="Guo X" first="Xinghua" last="Guo">Xinghua Guo</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Malty, Ramy H" sort="Malty, Ramy H" uniqKey="Malty R" first="Ramy H" last="Malty">Ramy H. Malty</name><affiliation><nlm:affiliation>Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Sarov, Mihail" sort="Sarov, Mihail" uniqKey="Sarov M" first="Mihail" last="Sarov">Mihail Sarov</name><affiliation><nlm:affiliation>Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Greenblatt, Jack" sort="Greenblatt, Jack" uniqKey="Greenblatt J" first="Jack" last="Greenblatt">Jack Greenblatt</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Babu, Mohan" sort="Babu, Mohan" uniqKey="Babu M" first="Mohan" last="Babu">Mohan Babu</name><affiliation><nlm:affiliation>Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Derry, W Brent" sort="Derry, W Brent" uniqKey="Derry W" first="W Brent" last="Derry">W Brent Derry</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Tillier, Elisabeth R" sort="Tillier, Elisabeth R" uniqKey="Tillier E" first="Elisabeth R" last="Tillier">Elisabeth R. Tillier</name><affiliation><nlm:affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Wallingford, John B" sort="Wallingford, John B" uniqKey="Wallingford J" first="John B" last="Wallingford">John B. Wallingford</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Marcotte, Edward M" sort="Marcotte, Edward M" uniqKey="Marcotte E" first="Edward M" last="Marcotte">Edward M. Marcotte</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Emili, Andrew" sort="Emili, Andrew" uniqKey="Emili A" first="Andrew" last="Emili">Andrew Emili</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26344197</idno><idno type="pmid">26344197</idno><idno type="doi">10.1038/nature14877</idno><idno type="wicri:Area/PubMed/Corpus">000415</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000415</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Panorama of ancient metazoan macromolecular complexes.</title><author><name sortKey="Wan, Cuihong" sort="Wan, Cuihong" uniqKey="Wan C" first="Cuihong" last="Wan">Cuihong Wan</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Borgeson, Blake" sort="Borgeson, Blake" uniqKey="Borgeson B" first="Blake" last="Borgeson">Blake Borgeson</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Phanse, Sadhna" sort="Phanse, Sadhna" uniqKey="Phanse S" first="Sadhna" last="Phanse">Sadhna Phanse</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Tu, Fan" sort="Tu, Fan" uniqKey="Tu F" first="Fan" last="Tu">Fan Tu</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Drew, Kevin" sort="Drew, Kevin" uniqKey="Drew K" first="Kevin" last="Drew">Kevin Drew</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Clark, Greg" sort="Clark, Greg" uniqKey="Clark G" first="Greg" last="Clark">Greg Clark</name><affiliation><nlm:affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Xiong, Xuejian" sort="Xiong, Xuejian" uniqKey="Xiong X" first="Xuejian" last="Xiong">Xuejian Xiong</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Kagan, Olga" sort="Kagan, Olga" uniqKey="Kagan O" first="Olga" last="Kagan">Olga Kagan</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Kwan, Julian" sort="Kwan, Julian" uniqKey="Kwan J" first="Julian" last="Kwan">Julian Kwan</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Bezginov, Alexandr" sort="Bezginov, Alexandr" uniqKey="Bezginov A" first="Alexandr" last="Bezginov">Alexandr Bezginov</name><affiliation><nlm:affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Chessman, Kyle" sort="Chessman, Kyle" uniqKey="Chessman K" first="Kyle" last="Chessman">Kyle Chessman</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Pal, Swati" sort="Pal, Swati" uniqKey="Pal S" first="Swati" last="Pal">Swati Pal</name><affiliation><nlm:affiliation>Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Cromar, Graham" sort="Cromar, Graham" uniqKey="Cromar G" first="Graham" last="Cromar">Graham Cromar</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Papoulas, Ophelia" sort="Papoulas, Ophelia" uniqKey="Papoulas O" first="Ophelia" last="Papoulas">Ophelia Papoulas</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ni, Zuyao" sort="Ni, Zuyao" uniqKey="Ni Z" first="Zuyao" last="Ni">Zuyao Ni</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Boutz, Daniel R" sort="Boutz, Daniel R" uniqKey="Boutz D" first="Daniel R" last="Boutz">Daniel R. Boutz</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Stoilova, Snejana" sort="Stoilova, Snejana" uniqKey="Stoilova S" first="Snejana" last="Stoilova">Snejana Stoilova</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Havugimana, Pierre C" sort="Havugimana, Pierre C" uniqKey="Havugimana P" first="Pierre C" last="Havugimana">Pierre C. Havugimana</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Xinghua" sort="Guo, Xinghua" uniqKey="Guo X" first="Xinghua" last="Guo">Xinghua Guo</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Malty, Ramy H" sort="Malty, Ramy H" uniqKey="Malty R" first="Ramy H" last="Malty">Ramy H. Malty</name><affiliation><nlm:affiliation>Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Sarov, Mihail" sort="Sarov, Mihail" uniqKey="Sarov M" first="Mihail" last="Sarov">Mihail Sarov</name><affiliation><nlm:affiliation>Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Greenblatt, Jack" sort="Greenblatt, Jack" uniqKey="Greenblatt J" first="Jack" last="Greenblatt">Jack Greenblatt</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Babu, Mohan" sort="Babu, Mohan" uniqKey="Babu M" first="Mohan" last="Babu">Mohan Babu</name><affiliation><nlm:affiliation>Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Derry, W Brent" sort="Derry, W Brent" uniqKey="Derry W" first="W Brent" last="Derry">W Brent Derry</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Tillier, Elisabeth R" sort="Tillier, Elisabeth R" uniqKey="Tillier E" first="Elisabeth R" last="Tillier">Elisabeth R. Tillier</name><affiliation><nlm:affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Wallingford, John B" sort="Wallingford, John B" uniqKey="Wallingford J" first="John B" last="Wallingford">John B. Wallingford</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Marcotte, Edward M" sort="Marcotte, Edward M" uniqKey="Marcotte E" first="Edward M" last="Marcotte">Edward M. Marcotte</name><affiliation><nlm:affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Emili, Andrew" sort="Emili, Andrew" uniqKey="Emili A" first="Andrew" last="Emili">Andrew Emili</name><affiliation><nlm:affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Nature</title><idno type="eISSN">1476-4687</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Datasets as Topic</term><term>Evolution, Molecular</term><term>Humans</term><term>Multiprotein Complexes (chemistry)</term><term>Multiprotein Complexes (metabolism)</term><term>Protein Interaction Mapping</term><term>Protein Interaction Maps</term><term>Reproducibility of Results</term><term>Systems Biology</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Multiprotein Complexes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Multiprotein Complexes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Datasets as Topic</term><term>Evolution, Molecular</term><term>Humans</term><term>Protein Interaction Mapping</term><term>Protein Interaction Maps</term><term>Reproducibility of Results</term><term>Systems Biology</term><term>Tandem Mass Spectrometry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26344197</PMID><DateCreated><Year>2015</Year><Month>09</Month><Day>19</Day></DateCreated><DateCompleted><Year>2015</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1476-4687</ISSN><JournalIssue CitedMedium="Internet"><Volume>525</Volume><Issue>7569</Issue><PubDate><Year>2015</Year><Month>Sep</Month><Day>17</Day></PubDate></JournalIssue><Title>Nature</Title><ISOAbbreviation>Nature</ISOAbbreviation></Journal><ArticleTitle>Panorama of ancient metazoan macromolecular complexes.</ArticleTitle><Pagination><MedlinePgn>339-44</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/nature14877</ELocationID><Abstract><AbstractText>Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wan</LastName><ForeName>Cuihong</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borgeson</LastName><ForeName>Blake</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Phanse</LastName><ForeName>Sadhna</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tu</LastName><ForeName>Fan</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Drew</LastName><ForeName>Kevin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Clark</LastName><ForeName>Greg</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Xuejian</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kagan</LastName><ForeName>Olga</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kwan</LastName><ForeName>Julian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bezginov</LastName><ForeName>Alexandr</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chessman</LastName><ForeName>Kyle</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hospital for Sick Children, Toronto, Ontario M5G 1X8, 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and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>F32GM112495</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 GM076536</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>DP1 GM106408</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 GM067779</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant><Grant><GrantID>F32 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RefType="CommentIn"><RefSource>Nat Methods. 2015 Nov;12(11):1011</RefSource><PMID Version="1">26824107</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Trends Biochem Sci. 2016 Jan;41(1):4-6</RefSource><PMID Version="1">26682499</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Biol. 2006;7(11):120</RefSource><PMID Version="1">17147767</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Trends Biotechnol. 2007 Oct;25(10):448-54</RefSource><PMID Version="1">17825444</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Psychiatry. 2012 Jan;17(1):4-7</RefSource><PMID Version="1">21826058</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Chem Biol. 2007 Sep;3(9):570-5</RefSource><PMID Version="1">17643112</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2011 Oct 28;147(3):690-703</RefSource><PMID Version="1">22036573</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phys Rev Lett. 2003 Sep 26;91(13):138701</RefSource><PMID Version="1">14525344</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2014 Jan;42(Database issue):D472-7</RefSource><PMID Version="1">24243840</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2004 Jan 23;303(5657):540-3</RefSource><PMID Version="1">14704431</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2015 Jan 23;347(6220):1260419</RefSource><PMID Version="1">25613900</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2006 Mar 30;440(7084):637-43</RefSource><PMID Version="1">16554755</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Cell Proteomics. 2013 Dec;12(12):3851-73</RefSource><PMID Version="1">24043423</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2006 Jan 1;34(Database issue):D535-9</RefSource><PMID Version="1">16381927</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Biotechnol. 2010 Dec;28(12):1248-50</RefSource><PMID Version="1">21139605</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Dev Cell. 2014 Feb 10;28(3):282-94</RefSource><PMID Version="1">24462187</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2005 Jun 10;280(23):22222-32</RefSource><PMID Version="1">15799966</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Res. 2004 Jun;14(6):1107-18</RefSource><PMID Version="1">15173116</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1974-9</RefSource><PMID Version="1">15687504</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 1998 Feb 6;92(3):291-4</RefSource><PMID Version="1">9476889</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2012 Aug 31;150(5):1068-81</RefSource><PMID Version="1">22939629</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Genet. 2006 Mar;38(3):285-93</RefSource><PMID Version="1">16501559</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2008 May 13;105(19):6959-64</RefSource><PMID Version="1">18474861</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Hum Genet. 2015 May;23(5):633-8</RefSource><PMID Version="1">24916641</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Syst Biol. 2012 Jan 17;8:565</RefSource><PMID Version="1">22252388</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2009 Feb;37(3):825-31</RefSource><PMID Version="1">19095691</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12763-8</RefSource><PMID Version="1">18725631</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2014 Nov 20;159(5):1212-26</RefSource><PMID Version="1">25416956</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Hum Mol Genet. 2002 Jan 15;11(2):165-73</RefSource><PMID Version="1">11809725</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2010 Jul;38(Web Server issue):W214-20</RefSource><PMID Version="1">20576703</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2000 Mar 24;287(5461):2204-15</RefSource><PMID Version="1">10731134</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 1992 May 1;256(5057):622-7</RefSource><PMID Version="1">1585174</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biol Evol. 2013 Feb;30(2):332-46</RefSource><PMID Version="1">22977115</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biol Cell. 2015 Jan 1;26(1):91-103</RefSource><PMID Version="1">25355947</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2002 May 23;417(6887):399-403</RefSource><PMID Version="1">12000970</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2011 May 27;145(5):787-99</RefSource><PMID Version="1">21620140</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Database (Oxford). 2010 Oct 12;2010:baq023</RefSource><PMID Version="1">20940177</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1283-8</RefSource><PMID Version="1">17227853</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2005 Sep 23;122(6):957-68</RefSource><PMID Version="1">16169070</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2013 Jan;41(Database issue):D808-15</RefSource><PMID Version="1">23203871</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Dev Cell. 2011 Apr 19;20(4):483-96</RefSource><PMID Version="1">21497761</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 1999 Dec 2;402(6761 Suppl):C47-52</RefSource><PMID Version="1">10591225</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2010 Jan;38(Database issue):D497-501</RefSource><PMID Version="1">19884131</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2014 May 29;509(7502):575-81</RefSource><PMID Version="1">24870542</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2005 Feb 3;433(7025):531-7</RefSource><PMID Version="1">15690043</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Physiol Cell Physiol. 2013 Jul 15;305(2):C129-30</RefSource><PMID Version="1">23677795</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Res. 2011 Jul;21(7):1109-21</RefSource><PMID Version="1">21536720</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Biol. 2009 Apr 28;7(4):e96</RefSource><PMID Version="1">19402753</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochem J. 2006 Aug 15;398(1):63-71</RefSource><PMID Version="1">16573520</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D066264" 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