The nematode Caenorhabditis elegans: a versatile model for the study of proteotoxicity and aging.
Identifieur interne : 000629 ( PubMed/Checkpoint ); précédent : 000628; suivant : 000630The nematode Caenorhabditis elegans: a versatile model for the study of proteotoxicity and aging.
Auteurs : Yuli Volovik [Israël] ; Filipa Carvalhal Marques [Portugal] ; Ehud Cohen [Israël]Source :
- Methods (San Diego, Calif.) [ 1095-9130 ] ; 2014.
English descriptors
- KwdEn :
- Aging (genetics), Aging (pathology), Alzheimer Disease (genetics), Animals, Caenorhabditis elegans (genetics), Caenorhabditis elegans (physiology), Humans, Models, Animal, Nerve Degeneration (genetics), Nerve Degeneration (pathology), Parkinson Disease (genetics), Parkinson Disease (pathology), Signal Transduction (genetics).
- MESH :
- genetics : Aging, Alzheimer Disease, Caenorhabditis elegans, Nerve Degeneration, Parkinson Disease, Signal Transduction.
- pathology : Aging, Nerve Degeneration, Parkinson Disease.
- physiology : Caenorhabditis elegans.
- Animals, Humans, Models, Animal.
Abstract
Toxicity arising from protein misfolding and aggregation (proteotoxicity) is tightly mechanistically linked to the emergence of late-onset neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Why these maladies manifest in late stages of life and what mechanisms protect the young organism from disease are key enigmas. The nematode Caenorhabditis elegans offers key advantages that enable systematic exploration of many cell biological and functional aspects of neurodegeneration-linked proteotoxicity. Here we review the abundantly used nematode-based proteotoxicity models and delineate common techniques for the measurement of protein aggregation and rate of proteotoxicity. We also discuss the advantages offered by the worm for genetic screening, drug development and for the exploration of the links between proteotoxicity and the aging process.
DOI: 10.1016/j.ymeth.2014.04.014
PubMed: 24794346
Affiliations:
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Why these maladies manifest in late stages of life and what mechanisms protect the young organism from disease are key enigmas. The nematode Caenorhabditis elegans offers key advantages that enable systematic exploration of many cell biological and functional aspects of neurodegeneration-linked proteotoxicity. Here we review the abundantly used nematode-based proteotoxicity models and delineate common techniques for the measurement of protein aggregation and rate of proteotoxicity. We also discuss the advantages offered by the worm for genetic screening, drug development and for the exploration of the links between proteotoxicity and the aging process.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24794346</PMID><DateCreated><Year>2014</Year><Month>07</Month><Day>28</Day></DateCreated><DateCompleted><Year>2015</Year><Month>04</Month><Day>03</Day></DateCompleted><DateRevised><Year>2014</Year><Month>07</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9130</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Aug</Month><Day>01</Day></PubDate></JournalIssue><Title>Methods (San Diego, Calif.)</Title><ISOAbbreviation>Methods</ISOAbbreviation></Journal><ArticleTitle>The nematode Caenorhabditis elegans: a versatile model for the study of proteotoxicity and aging.</ArticleTitle><Pagination><MedlinePgn>458-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ymeth.2014.04.014</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1046-2023(14)00167-4</ELocationID><Abstract><AbstractText>Toxicity arising from protein misfolding and aggregation (proteotoxicity) is tightly mechanistically linked to the emergence of late-onset neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Why these maladies manifest in late stages of life and what mechanisms protect the young organism from disease are key enigmas. The nematode Caenorhabditis elegans offers key advantages that enable systematic exploration of many cell biological and functional aspects of neurodegeneration-linked proteotoxicity. Here we review the abundantly used nematode-based proteotoxicity models and delineate common techniques for the measurement of protein aggregation and rate of proteotoxicity. We also discuss the advantages offered by the worm for genetic screening, drug development and for the exploration of the links between proteotoxicity and the aging process.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Inc. 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