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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of the Protein Kinase A-Regulated Proteome of <italic>Cryptococcus neoformans</italic> Identifies a Role for the Ubiquitin-Proteasome Pathway in Capsule Formation</title><author><name sortKey="Geddes, J M H" sort="Geddes, J M H" uniqKey="Geddes J" first="J. M. H." last="Geddes">J. M. H. Geddes</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Caza, M" sort="Caza, M" uniqKey="Caza M" first="M." last="Caza">M. Caza</name><affiliation><nlm:aff id="aff2"><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Croll, D" sort="Croll, D" uniqKey="Croll D" first="D." last="Croll">D. Croll</name><affiliation><nlm:aff id="aff2"><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Stoynov, N" sort="Stoynov, N" uniqKey="Stoynov N" first="N." last="Stoynov">N. Stoynov</name><affiliation><nlm:aff id="aff3"><addr-line>Centre for High-throughput Biology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Foster, L J" sort="Foster, L J" uniqKey="Foster L" first="L. J." last="Foster">L. J. Foster</name><affiliation><nlm:aff id="aff3"><addr-line>Centre for High-throughput Biology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kronstad, J W" sort="Kronstad, J W" uniqKey="Kronstad J" first="J. W." last="Kronstad">J. W. Kronstad</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26758180</idno><idno type="pmc">4725006</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725006</idno><idno type="RBID">PMC:4725006</idno><idno type="doi">10.1128/mBio.01862-15</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000163</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000163</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Analysis of the Protein Kinase A-Regulated Proteome of <italic>Cryptococcus neoformans</italic> Identifies a Role for the Ubiquitin-Proteasome Pathway in Capsule Formation</title><author><name sortKey="Geddes, J M H" sort="Geddes, J M H" uniqKey="Geddes J" first="J. M. H." last="Geddes">J. M. H. Geddes</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Caza, M" sort="Caza, M" uniqKey="Caza M" first="M." last="Caza">M. Caza</name><affiliation><nlm:aff id="aff2"><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Croll, D" sort="Croll, D" uniqKey="Croll D" first="D." last="Croll">D. Croll</name><affiliation><nlm:aff id="aff2"><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Stoynov, N" sort="Stoynov, N" uniqKey="Stoynov N" first="N." last="Stoynov">N. Stoynov</name><affiliation><nlm:aff id="aff3"><addr-line>Centre for High-throughput Biology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Foster, L J" sort="Foster, L J" uniqKey="Foster L" first="L. J." last="Foster">L. J. Foster</name><affiliation><nlm:aff id="aff3"><addr-line>Centre for High-throughput Biology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kronstad, J W" sort="Kronstad, J W" uniqKey="Kronstad J" first="J. W." last="Kronstad">J. W. Kronstad</name><affiliation><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">mBio</title><idno type="eISSN">2150-7511</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p>The opportunistic fungal pathogen <italic>Cryptococcus neoformans</italic> causes life-threatening meningitis in immunocompromised individuals. The expression of virulence factors, including capsule and melanin, is in part regulated by the cyclic-AMP/protein kinase A (cAMP/PKA) signal transduction pathway. In this study, we investigated the influence of PKA on the composition of the intracellular proteome to obtain a comprehensive understanding of the regulation that underpins virulence. Through quantitative proteomics, enrichment and bioinformatic analyses, and an interactome study, we uncovered a pattern of PKA regulation for proteins associated with translation, the proteasome, metabolism, amino acid biosynthesis, and virulence-related functions. PKA regulation of the ubiquitin-proteasome pathway in <italic>C. neoformans</italic> showed a striking parallel with connections between PKA and protein degradation in chronic neurodegenerative disorders and other human diseases. Further investigation of proteasome function with the inhibitor bortezomib revealed an impact on capsule production as well as hypersusceptibility for strains with altered expression or activity of PKA. Parallel studies with tunicamycin also linked endoplasmic reticulum stress with capsule production and PKA. Taken together, the data suggest a model whereby expression of PKA regulatory and catalytic subunits and the activation of PKA influence proteostasis and the function of the endoplasmic reticulum to control the elaboration of the polysaccharide capsule. Overall, this study revealed both broad and conserved influences of the cAMP/PKA pathway on the proteome and identified proteostasis as a potential therapeutic target for the treatment of cryptococcosis.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Mitchell, Tg" uniqKey="Mitchell T">TG Mitchell</name></author><author><name sortKey="Perfect, Jr" uniqKey="Perfect J">JR Perfect</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Bj" uniqKey="Park B">BJ Park</name></author><author><name sortKey="Wannemuehler, Ka" uniqKey="Wannemuehler K">KA Wannemuehler</name></author><author><name sortKey="Marston, Bj" uniqKey="Marston B">BJ Marston</name></author><author><name sortKey="Govender, N" uniqKey="Govender N">N Govender</name></author><author><name sortKey="Pappas, Pg" uniqKey="Pappas P">PG Pappas</name></author><author><name sortKey="Chiller, Tm" uniqKey="Chiller T">TM 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<front><journal-meta><journal-id journal-id-type="nlm-ta">mBio</journal-id><journal-id journal-id-type="iso-abbrev">MBio</journal-id><journal-id journal-id-type="hwp">mbio</journal-id><journal-id journal-id-type="pmc">mbio</journal-id><journal-id journal-id-type="publisher-id">mBio</journal-id><journal-title-group><journal-title>mBio</journal-title></journal-title-group><issn pub-type="epub">2150-7511</issn><publisher><publisher-name>American Society of Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26758180</article-id><article-id pub-id-type="pmc">4725006</article-id><article-id pub-id-type="publisher-id">mBio01862-15</article-id><article-id pub-id-type="doi">10.1128/mBio.01862-15</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Analysis of the Protein Kinase A-Regulated Proteome of <italic>Cryptococcus neoformans</italic> Identifies a Role for the Ubiquitin-Proteasome Pathway in Capsule Formation</article-title><alt-title alt-title-type="running-head">Regulation of the Proteome of <italic>C</italic>. <italic>neoformans</italic> by PKA</alt-title><alt-title alt-title-type="short-authors">Geddes et al.</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Geddes</surname><given-names>J. M. H.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Caza</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Croll</surname><given-names>D.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="author-notes" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Stoynov</surname><given-names>N.</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Foster</surname><given-names>L. J.</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Kronstad</surname><given-names>J. W.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib></contrib-group><aff id="aff1"><label>a</label><addr-line>Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada</addr-line></aff><aff id="aff2"><label>b</label><addr-line>Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada</addr-line></aff><aff id="aff3"><label>c</label><addr-line>Centre for High-throughput Biology, University of British Columbia, Vancouver, BC, Canada</addr-line></aff><author-notes><corresp id="cor1">Address correspondence to J. W. Kronstad, <email>kronstad@msl.ubc.ca</email>.</corresp><fn id="fn1" fn-type="present-address"><label>*</label><p>Present address: D. Croll, Institute of Integrative Biology, ETH Zürich, Zürich, Switzerland.</p></fn><fn fn-type="edited-by"><p><bold>Invited Editor</bold> Andrew Alspaugh, Duke University Medical Center <bold>Editor</bold> Françoise Dromer, Institut Pasteur</p></fn><fn fn-type="other"><p>This article is a direct contribution from a Fellow of the American Academy of Microbiology.</p></fn></author-notes><pub-date pub-type="epub"><day>12</day><month>1</month><year>2016</year></pub-date><pub-date pub-type="collection"><season>Jan-Feb</season><year>2016</year></pub-date><volume>7</volume><issue>1</issue><elocation-id>e01862-15</elocation-id><history><date date-type="received"><day>27</day><month>10</month><year>2015</year></date><date date-type="accepted"><day>9</day><month>11</month><year>2015</year></date></history><permissions><copyright-statement>Copyright © 2016 Geddes et al.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Geddes et al.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/"><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license</ext-link>, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="mbo001162588001.pdf"></self-uri><abstract><title>ABSTRACT</title><p>The opportunistic fungal pathogen <italic>Cryptococcus neoformans</italic> causes life-threatening meningitis in immunocompromised individuals. The expression of virulence factors, including capsule and melanin, is in part regulated by the cyclic-AMP/protein kinase A (cAMP/PKA) signal transduction pathway. In this study, we investigated the influence of PKA on the composition of the intracellular proteome to obtain a comprehensive understanding of the regulation that underpins virulence. Through quantitative proteomics, enrichment and bioinformatic analyses, and an interactome study, we uncovered a pattern of PKA regulation for proteins associated with translation, the proteasome, metabolism, amino acid biosynthesis, and virulence-related functions. PKA regulation of the ubiquitin-proteasome pathway in <italic>C. neoformans</italic> showed a striking parallel with connections between PKA and protein degradation in chronic neurodegenerative disorders and other human diseases. Further investigation of proteasome function with the inhibitor bortezomib revealed an impact on capsule production as well as hypersusceptibility for strains with altered expression or activity of PKA. Parallel studies with tunicamycin also linked endoplasmic reticulum stress with capsule production and PKA. Taken together, the data suggest a model whereby expression of PKA regulatory and catalytic subunits and the activation of PKA influence proteostasis and the function of the endoplasmic reticulum to control the elaboration of the polysaccharide capsule. Overall, this study revealed both broad and conserved influences of the cAMP/PKA pathway on the proteome and identified proteostasis as a potential therapeutic target for the treatment of cryptococcosis.</p></abstract><abstract abstract-type="executive-summary"><title>IMPORTANCE</title><p>Fungi cause life-threatening diseases, but very few drugs are available to effectively treat fungal infections. The pathogenic fungus <italic>Cryptococcus neoformans</italic> causes a substantial global burden of life-threatening meningitis in patients suffering from HIV/AIDS. An understanding of the mechanisms by which fungi deploy virulence factors to cause disease is critical for developing new therapeutic approaches. We employed a quantitative proteomic approach to define the changes in the protein complement that occur upon modulating the cAMP signaling pathway that regulates virulence in <italic>C. neoformans</italic>. This approach identified a conserved role for cAMP signaling in the regulation of the ubiquitin-proteasome pathway and revealed a link between this pathway and elaboration of a major virulence determinant, the polysaccharide capsule. Targeting the ubiquitin-proteasome pathway opens new therapeutic options for the treatment of cryptococcosis.</p></abstract><counts><count count-type="supplementary-material" count="9"></count><fig-count count="6"></fig-count><table-count count="3"></table-count><equation-count count="0"></equation-count><ref-count count="81"></ref-count><page-count count="15"></page-count><word-count count="12414"></word-count></counts><custom-meta-group><custom-meta><meta-name>cover-date</meta-name><meta-value>January/February 2016</meta-value></custom-meta></custom-meta-group></article-meta></front><body><sec sec-type="intro" id="h0.0"><title>INTRODUCTION</title><p>The opportunistic fungal pathogen <italic>Cryptococcus neoformans</italic> causes cryptococcal meningitis in immunocompromised individuals, with a high level of morbidity and mortality in patients suffering from HIV/AIDS (<xref rid="B1" ref-type="bibr">1</xref>, <xref rid="B2" ref-type="bibr">2</xref>). The virulence of the fungus is attributed to its ability to grow at 37°C, to produce a polysaccharide capsule and melanin, and to secrete extracellular enzymes and proteins that facilitate proliferation within the host (<xref rid="B3" ref-type="bibr">3</xref><xref ref-type="bibr" rid="B4">–</xref><xref rid="B8" ref-type="bibr">8</xref>). The capsule is a major virulence factor, and the constituent polysaccharides are synthesized intracellularly, exported to the cell surface, and attached to the cell wall to contribute immunomodulatory and antiphagocytic properties during infection (<xref rid="B9" ref-type="bibr">9</xref><xref ref-type="bibr" rid="B10">–</xref><xref rid="B15" ref-type="bibr">15</xref>). In <italic>C. neoformans</italic>, the cyclic-AMP/protein kinase A (cAMP/PKA) signal transduction pathway is a key regulator of virulence factor elaboration and may also regulate the trafficking of virulence components to the cell surface (<xref rid="B16" ref-type="bibr">16</xref>). In particular, PKA activity is known to regulate capsule production and melanin formation (<xref rid="B17" ref-type="bibr">17</xref><xref ref-type="bibr" rid="B18">–</xref><xref rid="B21" ref-type="bibr">21</xref>). Environmental signals, such as exogenous methionine and nutrient starvation, are capable of activating the pathway via a G-protein coupled receptor, Gpr4 (<xref rid="B22" ref-type="bibr">22</xref><xref ref-type="bibr" rid="B23">–</xref><xref rid="B24" ref-type="bibr">24</xref>). Mutants lacking Pka1, the catalytic subunit of PKA, show a reduction in levels of capsule and melanin formation, along with sterility and virulence attenuation, in a mouse model of cryptococcosis (<xref rid="B19" ref-type="bibr">19</xref>, <xref rid="B25" ref-type="bibr">25</xref>). In contrast, loss of Pkr1, the regulatory subunit of PKA, results in cells with an enlarged-capsule phenotype and hypervirulence (<xref rid="B19" ref-type="bibr">19</xref>).</p><p>In general, the underlying mechanisms and targets of cAMP/PKA regulation of virulence in <italic>C. neoformans</italic> remain to be characterized in detail. The influence of the cAMP pathway on the transcriptome was initially characterized by profiling a mutant defective in the Gpa1 Gα protein that activates adenylate cyclase (<xref rid="B26" ref-type="bibr">26</xref>). That work revealed pathway regulation of genes for capsule assembly and the genes encoding laccases for melanin formation. The influence of PKA on the transcriptome was also studied with <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants (<xref rid="B16" ref-type="bibr">16</xref>). That analysis revealed an influence of PKA on transcript levels for genes related to cell wall synthesis, transport, metabolism, and glycolysis, as well as for genes encoding ribosomal proteins, stress and chaperone functions, and components of the secretory pathway. Importantly, PKA has been shown to influence capsule attachment to the cell wall via regulation of the pH-responsive transcription factor Rim101, a key regulator of cell wall functions (<xref rid="B27" ref-type="bibr">27</xref>). The recent construction and characterization of galactose-inducible and glucose-repressible versions of <italic>PKA1</italic> in <italic>C. neoformans</italic> by inserting the <italic>GAL7</italic> promoter upstream of the gene provides a further opportunity to investigate the influence of PKA activity on virulence traits (<xref rid="B28" ref-type="bibr">28</xref>). The initial use of the regulated strains showed that galactose induction of <italic>PKA1</italic> influences capsule thickness, cell size, ploidy, vacuole enlargement, and melaninization and laccase activity, as well as the secretion of proteases and urease. The galactose-inducible and glucose-repressible <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain was also used recently to characterize changes in the secretome of <italic>C. neoformans</italic> in response to modulation of <italic>PKA1</italic> expression (<xref rid="B29" ref-type="bibr">29</xref>).</p><p>In the present study, we used the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain to investigate the influence of <italic>PKA1</italic> modulation on the intracellular proteome. Specifically, we employed quantitative proteomics to analyze protein abundance, and we identified 302 proteins that were regulated by Pka1 and that covered a broad spectrum of biological processes. Through enrichment and bioinformatic analyses and an interactome study, we discovered a conserved pattern of regulation by Pka1 for proteins associated with translation, the ubiquitin-proteasome pathway (UPP), metabolism, amino acid biosynthesis, and virulence-related functions. The Pka1 influence on the UPP in <italic>C. neoformans</italic> was particularly striking because of the established connection between this pathway, PKA activity, and protein degradation in chronic neurodegenerative disorders and other human diseases. These diseases are associated with impaired protein turnover and the accumulation of intracellular ubiquitin-protein aggregates, which are linked to changes in PKA activity (<xref rid="B30" ref-type="bibr">30</xref>, <xref rid="B31" ref-type="bibr">31</xref>). We investigated the role of the proteasome further in <italic>C. neoformans</italic> by using the inhibitor bortezomib to confirm the connection with PKA and to demonstrate a reduction in both capsule production and fungal growth. Bortezomib is an FDA-approved, boronic acid-based proteasome inhibitor that is widely used as an anticancer drug for the treatment of multiple myeloma (<xref rid="B32" ref-type="bibr">32</xref>). In parallel, we also established that strains with altered PKA expression or activity have altered susceptibility to tunicamycin, thereby revealing a connection with endoplasmic reticulum (ER) stress. Overall, this study advanced our understanding of the influence of the cAMP/PKA pathway on the cryptococcal proteome and identified proteostasis as a potential therapeutic avenue for the treatment of cryptococcosis.</p></sec><sec sec-type="results" id="h1"><title>RESULTS</title><sec id="s1.1"><title>Quantitative profiling of the proteome upon modulation of <italic>PKA1</italic> expression.</title><p>Given the virulence defect of a <italic>pka1</italic> mutant and Pka1 regulation of secreted virulence factors in <italic>C. neoformans</italic>, we hypothesized that Pka1 influences the abundance of proteins associated with these functions as well as general processes such as translation and metabolism (<xref rid="B19" ref-type="bibr">19</xref>). We therefore evaluated the effect of Pka1 regulation on the proteome by collecting cells of the wild-type (WT) and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains grown under Pka1-repressed (glucose) and Pka1-induced (galactose) conditions at 16 h postinoculation (hpi). To account for any changes associated with the use of different carbon sources (glucose versus galactose) to repress or induce <italic>PKA1</italic> expression, the WT strain was grown under the same conditions and comparisons were performed between strains from the same carbon source. We analyzed the samples using quantitative mass spectrometry and identified 3,222 proteins representing 48.1% of the 6,692 predicted proteins from the genome sequence (<xref rid="B33" ref-type="bibr">33</xref>). A total of 1,453 and 1,435 proteins were identified and quantified by dimethyl labeling in two or more replicates under Pka1-repressed and Pka1-induced conditions, respectively; 1,176 proteins were found under both conditions. A comparison of Gene Ontology (GO) biological-classification terms for the unique cellular proteins identified under either repressed conditions (277 proteins) or induced conditions (259 proteins) revealed changes in the proteome profiles in the strains responding to modulation of Pka1 activity (<xref ref-type="fig" rid="fig1">Fig. 1</xref>). The majority of proteins under both repressed and induced conditions (61% and 63%, respectively) were associated with metabolic, catabolic, and biosynthetic processes or were unknown or unclassified (including hypothetical proteins) (<xref ref-type="fig" rid="fig1">Fig. 1</xref>). Overall, the analysis of protein abundance in the <italic>PKA1</italic>-modulated strain revealed an impact of Pka1 on a diverse array of cellular processes.</p><fig id="fig1" orientation="portrait" position="float"><label>FIG 1 </label><caption><p>Modulation of Pka1 expression changes the proteome profile of <italic>C. neoformans</italic>. The pie charts indicate unique proteins identified and quantified under Pka1-repressed conditions (glucose-containing medium) and Pka1-induced conditions (galactose-containing medium) and categorized according to their GO term biological classifications (shown on the right).</p></caption><graphic xlink:href="mbo0061525880001"></graphic></fig></sec><sec id="s1.2"><title>Identification of Pka1-regulated proteins.</title><p>We next identified the proteins regulated by Pka1 within the set of 1,176 proteins that were quantified and shared under the repressed and induced conditions. We examined the list of shared proteins and found 302 that passed a nonadjusted Student’s <italic>t</italic> test with regard to altered abundance in response to Pka1 regulation (see <xref ref-type="supplementary-material" rid="tabS1">Table S1</xref> in the supplemental material). We also adjusted the significance levels to account for the multiple-testing hypothesis, and this more stringent analysis revealed 40 Pka1-regulated proteins with the highest statistical significance; these proteins are indicated with asterisks in the tables in this article. The broader set of 302 Pka1-regulated proteins identified in our analysis covered a diverse spectrum of GO term biological classifications (14 categories), including metabolic, catabolic, biosynthetic, and cellular processes, transcription and translation, proteolysis and protein folding, oxidation-reduction, response to stress, transport, phosphorylation/dephosphorylation, signaling and signal transduction, hypothetical proteins, and unknown/unclassified proteins. A density plot comparing the proteins identified under Pka1-repressed and Pka1-induced conditions highlighted the influence of modulation of Pka1 activity. Remarkably, this approach revealed that the majority of regulated proteins decreased in abundance upon induction of <italic>PKA1</italic> (<xref ref-type="fig" rid="fig2">Fig. 2A</xref>). A closer inspection of the GO term biological processes confirmed that the majority of the proteins in all 14 categories showed a decrease in abundance upon <italic>PKA1</italic> induction (<xref ref-type="fig" rid="fig2">Fig. 2B</xref>). In contrast, induction of <italic>PKA1</italic> expression caused an increase in protein abundance for only a small number of proteins in the same categories of metabolic and biosynthetic processes, proteolysis and protein folding, and phosphorylation/dephosphorylation, as well as for hypothetical, unknown, or unclassified proteins (<xref ref-type="fig" rid="fig2">Fig. 2B</xref>). The overall patterns were supported by additional analyses performed using GO term classifications and enrichment, as well as KEGG pathway analysis (see <xref ref-type="supplementary-material" rid="figS1">Fig. S1</xref> and <xref ref-type="supplementary-material" rid="tabS1">Tables S1</xref>, <xref ref-type="supplementary-material" rid="tabS2">S2</xref>, and <xref ref-type="supplementary-material" rid="tabS3">S3</xref> in the supplemental material).</p><fig id="fig2" orientation="portrait" position="float"><label>FIG 2 </label><caption><p>Modulation of Pka1 expression influences the abundance of 302 proteins. (A) Density scatterplot of proteins whose abundance changed upon repression or induction of <italic>PKA1</italic> expression. Proteins were identified in two or more replicates under Pka1-repressed (glucose) conditions and Pka1-induced (galactose) conditions. Normalized fold change values for the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain versus the WT strain are presented. Statistical analysis performed using a Student’s <italic>t</italic> test identified 302 proteins whose results were significantly different (<italic>P</italic> < 0.05) under the two sets of conditions (green labels). A subset of 40 of these proteins showed a significant difference following correction for multiple-hypothesis testing using the Benjamini-Hochberg method (FDR = 0.05) (red labels). (B) Numbers of Pka1-regulated proteins in different GO term categories that showed changes in abundance upon induction of <italic>PKA1</italic> expression<italic>.</italic> The proteins were classified based on GO terms for biological processes.</p></caption><graphic xlink:href="mbo0061525880002"></graphic></fig></sec><sec id="s1.3"><title>Several clusters of interactions are predicted by network mapping of Pka1-regulated proteins.</title><p>Given the diverse impacts of Pka1 on the <italic>C. neoformans</italic> proteome, including components of several metabolic pathways and cellular processes, we next evaluated the predicted interaction network of Pka1-regulated proteins using the STRING (Search Tool for Retrieval of Interacting Genes/Proteins) database. We mapped the identified Pka1-regulated proteins and found that 1,509 protein-protein interactions were predicted (<xref ref-type="fig" rid="fig3">Fig. 3</xref>). Several clusters of protein-protein interactions were prominent in the interaction network map, with the greatest number occurring among proteins associated with the ribosome and translation (<xref ref-type="table" rid="tab1">Table 1</xref>; see also <xref ref-type="supplementary-material" rid="tabS3">Table S3</xref> in the supplemental material). A second interaction cluster included proteins associated with the ubiquitin-proteasome pathway (UPP). Although this category was noted in the KEGG analysis, the network mapping highlighted the prominence of these proteins and revealed their association in the network with the proteins for translation (<xref ref-type="table" rid="tab2">Table 2</xref>). Additional clusters in the network were more diverse and included interactions between proteins associated with metabolic and biosynthetic processes, phosphorylation/dephosphorylation, oxidation-reduction, and other functions. Interactions were also predicted for proteins in categories associated with stress, signaling, secretion, and virulence (<xref ref-type="table" rid="tab3">Table 3</xref>). This group also included proteins with functions in protein sorting and folding, e.g., an ortholog of the chaperone GrpE, a prefoldin alpha subunit, and a peptidyl-prolyl <italic>cis</italic>-<italic>trans</italic>-isomerase. With regard to virulence functions, our analysis revealed PKA regulation of the Cig1 protein (heme acquisition) and an acid phosphatase, both of which were previously observed in the PKA-regulated secretome, as well as chaperones, catalase, urease, and a superoxide dismutase (<xref rid="B29" ref-type="bibr">29</xref>, <xref rid="B34" ref-type="bibr">34</xref><xref ref-type="bibr" rid="B35">–</xref><xref rid="B37" ref-type="bibr">37</xref>). Assays of the latter two enzymes and immunoblot analysis performed with anti-Hsp70 antibody supported the observed differences in the proteome data (see <xref ref-type="supplementary-material" rid="figS2">Fig. S2A</xref>, <xref ref-type="supplementary-material" rid="figS2">B</xref>, and <xref ref-type="supplementary-material" rid="figS2">C</xref> in the supplemental material). Similarly, our previous finding (that Pka1 induction reduced secreted urease activity) was consistent with the lower protein level for the enzyme observed in the current proteome analysis (<xref ref-type="table" rid="tab3">Table 3</xref>) (<xref rid="B28" ref-type="bibr">28</xref>). Protein-protein interactions were not predicted for ~85 proteins in the data set. In general, the protein-protein interaction network for Pka1-regulated proteins emphasized the significant impact of Pka1 on the regulation of ribosomal proteins and translation, the UPP, and metabolism. Furthermore, the diverse classification of hypothetical proteins provided an opportunity for novel protein discovery in the context of PKA regulation of the proteome.</p><fig id="fig3" orientation="portrait" position="float"><label>FIG 3 </label><caption><p>STRING analysis reveals an impact of Pka1 regulation on a diverse set of proteins as well as a prominent influence on translation and on the ubiquitin proteasome pathway. STRING was used to visualize predicted protein-protein interactions for the identified 300 Pka1-regulated proteins (<ext-link ext-link-type="uri" xlink:href="http://string-db.org">http://string-db.org</ext-link>) using the corresponding proteins from <italic>C. neoformans</italic> strain JEC21 in the database (<xref rid="B79" ref-type="bibr">79</xref>). Several clusters were identified from the network mapping, and examples include (i) proteins associated with ribosomes and translation, including small-subunit ribosomal protein S13e (CNAG_01153) and eukaryotic translation initiation factor 3 subunit F (CNAG_06563); (ii) proteins associated with the proteasome, including 26S proteasome regulatory subunit N2 (CNAG_06175) and nascent polypeptide-associated complex subunit α (CNAG_04985); and (iii) diverse proteins associated with metabolism, phosphorylation, and virulence, including urease (CNAG_05540) and acetyl-coenzyme A (CoA) C-acetyltransferase (CNAG_02918). Nodes directly linked to the input are indicated with colors other than white, and nodes of a higher iteration/depth are indicated with white. Edges or predicted functional links consist of up to eight lines, with a different color representing each type of evidence (e.g., neighborhood, gene fusion, cooccurrence, coexpression, experiments, databases, text mining, and homology).</p></caption><graphic xlink:href="mbo0061525880003"></graphic></fig><table-wrap id="tab1" orientation="portrait" position="float"><label>TABLE 1 </label><caption><p>Proteins associated with translation and RNA processing in the Pka1-regulated proteome of <italic>C. neoformans</italic></p></caption><table frame="hsides" rules="groups"><colgroup span="1"><col span="1"></col><col span="1"></col><col span="1"></col><col span="1"></col><col span="1"></col></colgroup><thead><tr><th rowspan="2" colspan="1">Gene<xref ref-type="table-fn" rid="ngtab1.1"><sup>a</sup></xref></th><th rowspan="2" colspan="1">Protein identification</th><th colspan="2" rowspan="1">Fold change<xref ref-type="table-fn" rid="ngtab1.2"><sup>b</sup></xref><hr></hr></th><th rowspan="2" colspan="1"><italic>P</italic> value<xref ref-type="table-fn" rid="ngtab1.3"><sup>c</sup></xref></th></tr><tr><th rowspan="1" colspan="1">Pka1 repression</th><th rowspan="1" colspan="1">Pka1 induction</th></tr></thead><tbody><tr><td rowspan="1" colspan="1">CNAG_00034</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L9e</td><td rowspan="1" colspan="1">0.363</td><td rowspan="1" colspan="1">−1.034</td><td rowspan="1" colspan="1">0.021</td></tr><tr><td rowspan="1" colspan="1">CNAG_00116</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S3</td><td rowspan="1" colspan="1">0.694</td><td rowspan="1" colspan="1">−1.646</td><td rowspan="1" colspan="1">0.010</td></tr><tr><td rowspan="1" colspan="1">CNAG_00640</td><td rowspan="1" colspan="1">40S ribosomal protein S4</td><td rowspan="1" colspan="1">1.011</td><td rowspan="1" colspan="1">−2.183</td><td rowspan="1" colspan="1">0.013</td></tr><tr><td rowspan="1" colspan="1">CNAG_00656</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L7e</td><td rowspan="1" colspan="1">0.728</td><td rowspan="1" colspan="1">−2.201</td><td rowspan="1" colspan="1">0.049</td></tr><tr><td rowspan="1" colspan="1">CNAG_00779</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L27e</td><td rowspan="1" colspan="1">2.050</td><td rowspan="1" colspan="1">−0.797</td><td rowspan="1" colspan="1">0.048</td></tr><tr><td rowspan="1" colspan="1">CNAG_00819</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S30</td><td rowspan="1" colspan="1">1.667</td><td rowspan="1" colspan="1">−3.881</td><td rowspan="1" colspan="1">0.034</td></tr><tr><td rowspan="1" colspan="1">CNAG_01152</td><td rowspan="1" colspan="1">40S ribosomal protein s6</td><td rowspan="1" colspan="1">0.869</td><td rowspan="1" colspan="1">−1.200</td><td rowspan="1" colspan="1">0.008</td></tr><tr><td rowspan="1" colspan="1">CNAG_01153*</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S13e</td><td rowspan="1" colspan="1">0.950</td><td rowspan="1" colspan="1">−1.257</td><td rowspan="1" colspan="1">0.002</td></tr><tr><td rowspan="1" colspan="1">CNAG_01181</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S27Ae</td><td rowspan="1" colspan="1">2.252</td><td rowspan="1" colspan="1">−0.510</td><td rowspan="1" colspan="1">0.025</td></tr><tr><td rowspan="1" colspan="1">CNAG_01300</td><td rowspan="1" colspan="1">40S ribosomal protein S21</td><td rowspan="1" colspan="1">0.966</td><td rowspan="1" colspan="1">−2.924</td><td rowspan="1" colspan="1">0.045</td></tr><tr><td rowspan="1" colspan="1">CNAG_01332</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S24e</td><td rowspan="1" colspan="1">1.318</td><td rowspan="1" colspan="1">−2.812</td><td rowspan="1" colspan="1">0.040</td></tr><tr><td rowspan="1" colspan="1">CNAG_01455</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L39</td><td rowspan="1" colspan="1">1.359</td><td rowspan="1" colspan="1">−2.756</td><td rowspan="1" colspan="1">0.027</td></tr><tr><td rowspan="1" colspan="1">CNAG_01480</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L12</td><td rowspan="1" colspan="1">0.259</td><td rowspan="1" colspan="1">−1.889</td><td rowspan="1" colspan="1">0.048</td></tr><tr><td rowspan="1" colspan="1">CNAG_01843</td><td rowspan="1" colspan="1">Elongation factor Ts; mitochondrial</td><td rowspan="1" colspan="1">1.066</td><td rowspan="1" colspan="1">−1.523</td><td rowspan="1" colspan="1">0.027</td></tr><tr><td rowspan="1" colspan="1">CNAG_01884</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L3</td><td rowspan="1" colspan="1">1.305</td><td rowspan="1" colspan="1">−1.408</td><td rowspan="1" colspan="1">0.019</td></tr><tr><td rowspan="1" colspan="1">CNAG_01897</td><td rowspan="1" colspan="1">Bromodomain-containing factor 1</td><td rowspan="1" colspan="1">0.102</td><td rowspan="1" colspan="1">−0.604</td><td rowspan="1" colspan="1">0.012</td></tr><tr><td rowspan="1" colspan="1">CNAG_01951</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S22-A</td><td rowspan="1" colspan="1">1.879</td><td rowspan="1" colspan="1">−1.756</td><td rowspan="1" colspan="1">0.027</td></tr><tr><td rowspan="1" colspan="1">CNAG_01990</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S5</td><td rowspan="1" colspan="1">0.584</td><td rowspan="1" colspan="1">−1.195</td><td rowspan="1" colspan="1">0.032</td></tr><tr><td rowspan="1" colspan="1">CNAG_02145</td><td rowspan="1" colspan="1">Uncharacterized protein</td><td rowspan="1" colspan="1">0.848</td><td rowspan="1" colspan="1">−2.701</td><td rowspan="1" colspan="1">0.008</td></tr><tr><td rowspan="1" colspan="1">CNAG_02209</td><td rowspan="1" colspan="1">Nucleolar protein 56</td><td rowspan="1" colspan="1">0.291</td><td rowspan="1" colspan="1">−1.459</td><td rowspan="1" colspan="1">0.049</td></tr><tr><td rowspan="1" colspan="1">CNAG_02234</td><td rowspan="1" colspan="1">60S ribosomal protein L6</td><td rowspan="1" colspan="1">0.984</td><td rowspan="1" colspan="1">−2.161</td><td rowspan="1" colspan="1">0.041</td></tr><tr><td rowspan="1" colspan="1">CNAG_02330</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L21e</td><td rowspan="1" colspan="1">1.433</td><td rowspan="1" colspan="1">−1.945</td><td rowspan="1" colspan="1">0.017</td></tr><tr><td rowspan="1" colspan="1">CNAG_02331</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S9</td><td rowspan="1" colspan="1">1.492</td><td rowspan="1" colspan="1">−1.643</td><td rowspan="1" colspan="1">0.047</td></tr><tr><td rowspan="1" colspan="1">CNAG_02671</td><td rowspan="1" colspan="1">Pre-mRNA splicing factor CEF1</td><td rowspan="1" colspan="1">1.137</td><td rowspan="1" colspan="1">−2.223</td><td rowspan="1" colspan="1">0.012</td></tr><tr><td rowspan="1" colspan="1">CNAG_03198</td><td rowspan="1" colspan="1">40S ribosomal protein S8</td><td rowspan="1" colspan="1">0.949</td><td rowspan="1" colspan="1">−1.396</td><td rowspan="1" colspan="1">0.016</td></tr><tr><td rowspan="1" colspan="1">CNAG_03283</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L24e</td><td rowspan="1" colspan="1">1.577</td><td rowspan="1" colspan="1">−4.493</td><td rowspan="1" colspan="1">0.032</td></tr><tr><td rowspan="1" colspan="1">CNAG_03577</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein LP0</td><td rowspan="1" colspan="1">0.332</td><td rowspan="1" colspan="1">−1.031</td><td rowspan="1" colspan="1">0.019</td></tr><tr><td rowspan="1" colspan="1">CNAG_03739</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L10-like</td><td rowspan="1" colspan="1">2.030</td><td rowspan="1" colspan="1">−3.596</td><td rowspan="1" colspan="1">0.027</td></tr><tr><td rowspan="1" colspan="1">CNAG_03747</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L27Ae</td><td rowspan="1" colspan="1">1.055</td><td rowspan="1" colspan="1">−2.462</td><td rowspan="1" colspan="1">0.046</td></tr><tr><td rowspan="1" colspan="1">CNAG_04004</td><td rowspan="1" colspan="1">40S ribosomal protein S1</td><td rowspan="1" colspan="1">1.442</td><td rowspan="1" colspan="1">−2.205</td><td rowspan="1" colspan="1">0.010</td></tr><tr><td rowspan="1" colspan="1">CNAG_04011</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L37a</td><td rowspan="1" colspan="1">1.441</td><td rowspan="1" colspan="1">−1.462</td><td rowspan="1" colspan="1">0.024</td></tr><tr><td rowspan="1" colspan="1">CNAG_04068</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L28e</td><td rowspan="1" colspan="1">1.210</td><td rowspan="1" colspan="1">−2.524</td><td rowspan="1" colspan="1">0.029</td></tr><tr><td rowspan="1" colspan="1">CNAG_04082</td><td rowspan="1" colspan="1">Proline-tRNA ligase</td><td rowspan="1" colspan="1">0.391</td><td rowspan="1" colspan="1">−0.988</td><td rowspan="1" colspan="1">0.026</td></tr><tr><td rowspan="1" colspan="1">CNAG_04445</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S7e</td><td rowspan="1" colspan="1">0.544</td><td rowspan="1" colspan="1">−2.111</td><td rowspan="1" colspan="1">0.048</td></tr><tr><td rowspan="1" colspan="1">CNAG_04448</td><td rowspan="1" colspan="1">Ribosomal protein L19</td><td rowspan="1" colspan="1">1.106</td><td rowspan="1" colspan="1">−2.674</td><td rowspan="1" colspan="1">0.014</td></tr><tr><td rowspan="1" colspan="1">CNAG_04609</td><td rowspan="1" colspan="1">Argonaute</td><td rowspan="1" colspan="1">0.864</td><td rowspan="1" colspan="1">−0.811</td><td rowspan="1" colspan="1">0.050</td></tr><tr><td rowspan="1" colspan="1">CNAG_04628</td><td rowspan="1" colspan="1">Eukaryotic translation initiation factor 6</td><td rowspan="1" colspan="1">1.085</td><td rowspan="1" colspan="1">−0.212</td><td rowspan="1" colspan="1">0.045</td></tr><tr><td rowspan="1" colspan="1">CNAG_04726</td><td rowspan="1" colspan="1">60S ribosomal protein L20</td><td rowspan="1" colspan="1">2.692</td><td rowspan="1" colspan="1">−2.196</td><td rowspan="1" colspan="1">0.022</td></tr><tr><td rowspan="1" colspan="1">CNAG_04762</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L4e</td><td rowspan="1" colspan="1">1.531</td><td rowspan="1" colspan="1">−2.273</td><td rowspan="1" colspan="1">0.034</td></tr><tr><td rowspan="1" colspan="1">CNAG_04883</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S18</td><td rowspan="1" colspan="1">0.760</td><td rowspan="1" colspan="1">−2.768</td><td rowspan="1" colspan="1">0.042</td></tr><tr><td rowspan="1" colspan="1">CNAG_05232</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L8</td><td rowspan="1" colspan="1">1.362</td><td rowspan="1" colspan="1">−2.096</td><td rowspan="1" colspan="1">0.031</td></tr><tr><td rowspan="1" colspan="1">CNAG_05416</td><td rowspan="1" colspan="1">Pre-mRNA processing protein 45</td><td rowspan="1" colspan="1">1.998</td><td rowspan="1" colspan="1">−1.781</td><td rowspan="1" colspan="1">0.020</td></tr><tr><td rowspan="1" colspan="1">CNAG_05525</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S26</td><td rowspan="1" colspan="1">2.900</td><td rowspan="1" colspan="1">−3.011</td><td rowspan="1" colspan="1">0.049</td></tr><tr><td rowspan="1" colspan="1">CNAG_05689</td><td rowspan="1" colspan="1">Pre-mRNA splicing factor SPF27</td><td rowspan="1" colspan="1">1.115</td><td rowspan="1" colspan="1">−1.774</td><td rowspan="1" colspan="1">0.034</td></tr><tr><td rowspan="1" colspan="1">CNAG_05762</td><td rowspan="1" colspan="1">Large-subunit acidic ribosomal protein P2</td><td rowspan="1" colspan="1">1.026</td><td rowspan="1" colspan="1">−0.994</td><td rowspan="1" colspan="1">0.021</td></tr><tr><td rowspan="1" colspan="1">CNAG_05904</td><td rowspan="1" colspan="1">Small-subunit ribosomal protein S14</td><td rowspan="1" colspan="1">2.288</td><td rowspan="1" colspan="1">−1.705</td><td rowspan="1" colspan="1">0.019</td></tr><tr><td rowspan="1" colspan="1">CNAG_06123</td><td rowspan="1" colspan="1">Leucine-tRNA ligase</td><td rowspan="1" colspan="1">0.352</td><td rowspan="1" colspan="1">−0.757</td><td rowspan="1" colspan="1">0.026</td></tr><tr><td rowspan="1" colspan="1">CNAG_06231</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L13</td><td rowspan="1" colspan="1">1.762</td><td rowspan="1" colspan="1">−2.044</td><td rowspan="1" colspan="1">0.045</td></tr><tr><td rowspan="1" colspan="1">CNAG_06563*</td><td rowspan="1" colspan="1">Eukaryotic translation initiation factor 3 subunit F</td><td rowspan="1" colspan="1">0.978</td><td rowspan="1" colspan="1">−1.397</td><td rowspan="1" colspan="1">0.001</td></tr><tr><td rowspan="1" colspan="1">CNAG_07839</td><td rowspan="1" colspan="1">Large-subunit ribosomal protein L11</td><td rowspan="1" colspan="1">2.105</td><td rowspan="1" colspan="1">−1.925</td><td rowspan="1" colspan="1">0.044</td></tr></tbody></table><table-wrap-foot><fn id="ngtab1.1"><label><sup>a</sup></label><p>Gene designations labeled with an asterisk (*) represent genes that encoded proteins that gave significant results after multiple hypothesis testing (FDR, <0.05).</p></fn><fn id="ngtab1.2"><label><sup>b</sup></label><p>Data represent <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain/WT strain normalized log<sub>2</sub> average fold change values for three replicates determined under the respective glucose (repression) or galactose (induction) conditions.</p></fn><fn id="ngtab1.3"><label><sup>c</sup></label><p>Statistical analysis of Pka1 repression and Pka1 induction values was performed using Student’s <italic>t</italic> test (<italic>P</italic> value, <0.05).</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab2" orientation="portrait" position="float"><label>TABLE 2 </label><caption><p>Proteins associated with the proteasome and ubiquitin pathways in the Pka1-regulated proteome of <italic>C. neoformans</italic></p></caption><table frame="hsides" rules="groups"><colgroup span="1"><col span="1"></col><col span="1"></col><col span="1"></col><col span="1"></col><col span="1"></col></colgroup><thead><tr><th rowspan="2" colspan="1">Gene</th><th rowspan="2" colspan="1">Protein identification</th><th colspan="2" rowspan="1">Fold change<xref ref-type="table-fn" rid="ngtab2.1"><sup>a</sup></xref><hr></hr></th><th rowspan="2" colspan="1"><italic>P</italic> value<xref ref-type="table-fn" rid="ngtab2.2"><sup>b</sup></xref></th></tr><tr><th rowspan="1" colspan="1">Pka1 repression</th><th rowspan="1" colspan="1">Pka1 induction</th></tr></thead><tbody><tr><td rowspan="1" colspan="1">CNAG_00136</td><td rowspan="1" colspan="1">Ubiquitin-activating enzyme E1</td><td rowspan="1" colspan="1">0.177</td><td rowspan="1" colspan="1">−1.216</td><td rowspan="1" colspan="1">0.006</td></tr><tr><td rowspan="1" colspan="1">CNAG_00180</td><td rowspan="1" colspan="1">Ubiquitin carboxyl-terminal hydrolase</td><td rowspan="1" colspan="1">1.090</td><td rowspan="1" colspan="1">−2.260</td><td rowspan="1" colspan="1">0.004</td></tr><tr><td rowspan="1" colspan="1">CNAG_00482</td><td rowspan="1" colspan="1">26S proteasome regulatory subunit N10</td><td rowspan="1" colspan="1">0.715</td><td rowspan="1" colspan="1">−1.531</td><td rowspan="1" colspan="1">0.028</td></tr><tr><td rowspan="1" colspan="1">CNAG_01861</td><td rowspan="1" colspan="1">26S proteasome non-ATPase regulatory subunit 10</td><td rowspan="1" colspan="1">0.569</td><td rowspan="1" colspan="1">−1.739</td><td rowspan="1" colspan="1">0.026</td></tr><tr><td rowspan="1" colspan="1">CNAG_01881</td><td rowspan="1" colspan="1">Molecular chaperone GrpE</td><td rowspan="1" colspan="1">1.557</td><td rowspan="1" colspan="1">−2.879</td><td rowspan="1" colspan="1">0.005</td></tr><tr><td rowspan="1" colspan="1">CNAG_01899</td><td rowspan="1" colspan="1">Prefoldin alpha subunit</td><td rowspan="1" colspan="1">1.574</td><td rowspan="1" colspan="1">−2.132</td><td rowspan="1" colspan="1">0.031</td></tr><tr><td rowspan="1" colspan="1">CNAG_02239</td><td rowspan="1" colspan="1">26S protease regulatory subunit 4</td><td rowspan="1" colspan="1">0.714</td><td rowspan="1" colspan="1">−2.185</td><td rowspan="1" colspan="1">0.035</td></tr><tr><td rowspan="1" colspan="1">CNAG_02725</td><td rowspan="1" colspan="1">20S proteasome subunit beta 2</td><td rowspan="1" colspan="1">0.594</td><td rowspan="1" colspan="1">−1.365</td><td rowspan="1" colspan="1">0.045</td></tr><tr><td rowspan="1" colspan="1">CNAG_02827</td><td rowspan="1" colspan="1">Ubiquitin-like protein Nedd8</td><td rowspan="1" colspan="1">0.764</td><td rowspan="1" colspan="1">−0.761</td><td rowspan="1" colspan="1">0.028</td></tr><tr><td rowspan="1" colspan="1">CNAG_03627</td><td rowspan="1" colspan="1">Peptidyl-prolyl <italic>cis</italic>-<italic>trans</italic> isomerase</td><td rowspan="1" colspan="1">0.648</td><td rowspan="1" colspan="1">−1.725</td><td rowspan="1" colspan="1">0.049</td></tr><tr><td rowspan="1" colspan="1">CNAG_03721</td><td rowspan="1" colspan="1">26S proteasome regulatory subunit N12</td><td rowspan="1" colspan="1">1.433</td><td rowspan="1" colspan="1">−2.933</td><td rowspan="1" colspan="1">0.049</td></tr><tr><td rowspan="1" colspan="1">CNAG_04014</td><td rowspan="1" colspan="1">26S proteasome regulatory subunit N9</td><td rowspan="1" colspan="1">0.379</td><td rowspan="1" colspan="1">−1.537</td><td rowspan="1" colspan="1">0.021</td></tr><tr><td rowspan="1" colspan="1">CNAG_04071</td><td rowspan="1" colspan="1">Proteasome subunit alpha type</td><td rowspan="1" colspan="1">1.093</td><td rowspan="1" colspan="1">−1.295</td><td rowspan="1" colspan="1">0.029</td></tr><tr><td rowspan="1" colspan="1">CNAG_04906</td><td rowspan="1" colspan="1">26S protease regulatory subunit 10B</td><td rowspan="1" colspan="1">0.519</td><td rowspan="1" colspan="1">−0.929</td><td rowspan="1" colspan="1">0.033</td></tr><tr><td rowspan="1" colspan="1">CNAG_06106</td><td rowspan="1" colspan="1">Chaperone regulator</td><td rowspan="1" colspan="1">−0.603</td><td rowspan="1" colspan="1">0.152</td><td rowspan="1" colspan="1">0.006</td></tr><tr><td rowspan="1" colspan="1">CNAG_06175</td><td rowspan="1" colspan="1">26S proteasome regulatory subunit N2</td><td rowspan="1" colspan="1">0.228</td><td rowspan="1" colspan="1">−2.166</td><td rowspan="1" colspan="1">0.031</td></tr><tr><td rowspan="1" colspan="1">CNAG_06602</td><td rowspan="1" colspan="1">Cysteine-type peptidase</td><td rowspan="1" colspan="1">1.580</td><td rowspan="1" colspan="1">−2.278</td><td rowspan="1" colspan="1">0.001</td></tr><tr><td rowspan="1" colspan="1">CNAG_07717</td><td rowspan="1" colspan="1">Ubiquitin carboxyl-terminal hydrolase</td><td rowspan="1" colspan="1">0.606</td><td rowspan="1" colspan="1">−0.705</td><td rowspan="1" colspan="1">0.003</td></tr><tr><td rowspan="1" colspan="1">CNAG_07719</td><td rowspan="1" colspan="1">26S protease regulatory subunit 7</td><td rowspan="1" colspan="1">0.168</td><td rowspan="1" colspan="1">−1.619</td><td rowspan="1" colspan="1">0.021</td></tr></tbody></table><table-wrap-foot><fn id="ngtab2.1"><label><sup>a</sup></label><p>Data represent <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain/WT strain normalized log<sub>2</sub> average fold change values for three replicates determined under the respective glucose (repression) or galactose (induction) conditions.</p></fn><fn id="ngtab2.2"><label><sup>b</sup></label><p>Statistical analysis of Pka1 repression and Pka1 induction values was performed using Student’s <italic>t</italic> test (<italic>P</italic> value, <0.05).</p></fn></table-wrap-foot></table-wrap><table-wrap id="tab3" orientation="portrait" position="float"><label>TABLE 3 </label><caption><p>Identification of proteins associated with response to stress, chaperone function, signaling, and virulence in the Pka1-regulated proteome of <italic>C. neoformans</italic></p></caption><table frame="hsides" rules="groups"><colgroup span="1"><col span="1"></col><col span="1"></col><col span="1"></col><col span="1"></col><col span="1"></col></colgroup><thead><tr><th rowspan="2" colspan="1">Gene<xref ref-type="table-fn" rid="ngtab3.1"><sup>a</sup></xref></th><th rowspan="2" colspan="1">Protein identification</th><th colspan="2" rowspan="1">Fold change<xref ref-type="table-fn" rid="ngtab3.2"><sup>b</sup></xref><hr></hr></th><th rowspan="2" colspan="1"><italic>P</italic> value<xref ref-type="table-fn" rid="ngtab3.3"><sup>c</sup></xref></th></tr><tr><th rowspan="1" colspan="1">Pka1 repression</th><th rowspan="1" colspan="1">Pka1 induction</th></tr></thead><tbody><tr><td rowspan="1" colspan="1">CNAG_01404</td><td rowspan="1" colspan="1">Hsp71-like protein</td><td rowspan="1" colspan="1">0.680</td><td rowspan="1" colspan="1">−1.014</td><td rowspan="1" colspan="1">0.007</td></tr><tr><td rowspan="1" colspan="1">CNAG_01446</td><td rowspan="1" colspan="1">Uncharacterized protein</td><td rowspan="1" colspan="1">1.639</td><td rowspan="1" colspan="1">−3.295</td><td rowspan="1" colspan="1">0.014</td></tr><tr><td rowspan="1" colspan="1">CNAG_01653*</td><td rowspan="1" colspan="1">Cytokine-inducing glycoprotein</td><td rowspan="1" colspan="1">−4.515</td><td rowspan="1" colspan="1">3.130</td><td rowspan="1" colspan="1">0.002</td></tr><tr><td rowspan="1" colspan="1">CNAG_01744</td><td rowspan="1" colspan="1">Phosphatase</td><td rowspan="1" colspan="1">0.934</td><td rowspan="1" colspan="1">−1.785</td><td rowspan="1" colspan="1">0.011</td></tr><tr><td rowspan="1" colspan="1">CNAG_01817*</td><td rowspan="1" colspan="1">Signal recognition particle receptor subunit alpha</td><td rowspan="1" colspan="1">0.967</td><td rowspan="1" colspan="1">−0.574</td><td rowspan="1" colspan="1">0.003</td></tr><tr><td rowspan="1" colspan="1">CNAG_02817</td><td rowspan="1" colspan="1">GTP-binding protein ypt2</td><td rowspan="1" colspan="1">0.872</td><td rowspan="1" colspan="1">−0.783</td><td rowspan="1" colspan="1">0.015</td></tr><tr><td rowspan="1" colspan="1">CNAG_03143*</td><td rowspan="1" colspan="1">Uncharacterized protein</td><td rowspan="1" colspan="1">1.239</td><td rowspan="1" colspan="1">−1.371</td><td rowspan="1" colspan="1">0.000</td></tr><tr><td rowspan="1" colspan="1">CNAG_03891</td><td rowspan="1" colspan="1">Hsp60-like protein</td><td rowspan="1" colspan="1">0.697</td><td rowspan="1" colspan="1">−0.770</td><td rowspan="1" colspan="1">0.021</td></tr><tr><td rowspan="1" colspan="1">CNAG_03985</td><td rowspan="1" colspan="1">Glutaredoxin</td><td rowspan="1" colspan="1">1.215</td><td rowspan="1" colspan="1">−2.359</td><td rowspan="1" colspan="1">0.009</td></tr><tr><td rowspan="1" colspan="1">CNAG_05218</td><td rowspan="1" colspan="1">Adenylyl cyclase-associated protein</td><td rowspan="1" colspan="1">1.294</td><td rowspan="1" colspan="1">−0.075</td><td rowspan="1" colspan="1">0.045</td></tr><tr><td rowspan="1" colspan="1">CNAG_05540</td><td rowspan="1" colspan="1">Urease</td><td rowspan="1" colspan="1">0.476</td><td rowspan="1" colspan="1">−1.777</td><td rowspan="1" colspan="1">0.033</td></tr><tr><td rowspan="1" colspan="1">CNAG_06208</td><td rowspan="1" colspan="1">Heat shock 70-kDa protein 4</td><td rowspan="1" colspan="1">0.844</td><td rowspan="1" colspan="1">−0.931</td><td rowspan="1" colspan="1">0.021</td></tr><tr><td rowspan="1" colspan="1">CNAG_06287</td><td rowspan="1" colspan="1">Glutathione peroxidase</td><td rowspan="1" colspan="1">0.746</td><td rowspan="1" colspan="1">−0.330</td><td rowspan="1" colspan="1">0.044</td></tr></tbody></table><table-wrap-foot><fn id="ngtab3.1"><label><sup>a</sup></label><p>Gene designations labeled with an asterisk (*) represent genes that encoded proteins that gave significant results after multiple hypothesis testing (FDR, <0.05).</p></fn><fn id="ngtab3.2"><label><sup>b</sup></label><p>Data represent <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain/WT strain normalized log<sub>2</sub> average fold change values for three replicates determined under the respective glucose (repression) or galactose (induction) conditions.</p></fn><fn id="ngtab3.3"><label><sup>c</sup></label><p>Statistical analysis of Pka1 repression and Pka1 induction values was performed using Student’s <italic>t</italic> test (<italic>P</italic> value, <0.05).</p></fn></table-wrap-foot></table-wrap></sec><sec id="s1.4"><title>Characterization of Pka1-regulated novel proteins.</title><p>Our quantitative proteomic analysis revealed that 64 (21%) of the 302 candidates for Pka1-regulated proteins were conserved hypothetical proteins. To better understand the potential functions of these proteins, we used bioinformatic analyses to predict subcellular location, secretion, protein function, and possible enzymatic classification and to identify homologues (see <xref ref-type="supplementary-material" rid="tabS4">Table S4</xref> in the supplemental material). The subcellular location prediction tools TargetP and TMHMM identified 14 proteins associated with the mitochondrion, along with one protein containing a transmembrane helix. We noted that mitochondrial functions also appeared prominently among the metabolic and biosynthetic proteins. None of the hypothetical proteins contained a predicted glycosylphosphatidylinositol (GPI) anchor, and only one contained an N-terminal signal peptide for conventional secretion. ProtFun identified proteins with cellular roles related to central intermediary metabolism, energy metabolism, amino acid biosynthesis, biosynthesis of cofactors, translation, regulation and transcription, regulatory functions, transport and binding, purines and pyrimidines, and the cell envelope. Forty candidate enzymes were identified, and eight were classifiable as lyases, three as ligases, and three as isomerases. Additional GO term categories were associated with growth factors, hormones, transcription and transcriptional regulation, immune response, voltage-gated ion channels, and structural proteins. Lastly, HMMER identified protein homologues, including NAD-, RNA-, and carbohydrate-binding proteins, enzymes (methyltransferase, glutathione-S-transferase, phosphatases, and a protein tyrosine kinase), a heat shock protein, translation/ribosomal proteins, and proteins associated with transcription and transcriptional repression. These results revealed a diverse classification of hypothetical proteins, along with the potential for novel protein discovery in the context of PKA regulation of the proteome. Interestingly, the two Hsp12 homologues that we identified are known to influence growth on amphotericin B and to be regulated by the cAMP and high-osmolarity glycerol (HOG) signaling pathways in <italic>C. neoformans</italic> (<xref rid="B16" ref-type="bibr">16</xref>, <xref rid="B38" ref-type="bibr">38</xref>). Some of the identified proteins may also contribute to virulence and proliferation in vertebrate hosts. For example, this may be the case for CNAG_03007, which is a homologue of the CipC protein, because the corresponding transcript was previously found to be highly abundant in cryptococcal cells obtained from the central nervous system of infected rabbits (<xref rid="B39" ref-type="bibr">39</xref>).</p></sec><sec id="s1.5"><title>Connections between inhibition of the proteasome, PKA, and capsule production.</title><p>As mentioned above, we found that induction of Pka1 expression decreased the abundance of multiple components of the UPP in <italic>C. neoformans</italic>, perhaps as part of a PKA-regulated remodeling of proteostasis. Given that one of the most dramatic consequences of elevated PKA activity is the enlargement of the polysaccharide capsule, we investigated the impact of a proteasome inhibitor, bortezomib, on capsule production in the WT strain, the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants, and the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain. This analysis revealed a reduction in the diameter of cell-associated capsule for the WT strain, the <italic>pkr1</italic>Δ mutant, and the induced <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain in the presence of the inhibitor (<xref ref-type="fig" rid="fig4">Fig. 4A</xref> and <xref ref-type="fig" rid="fig4">B</xref>). The cell sizes of the <italic>pkr1</italic>Δ mutant and the induced <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain were also decreased relative to the sizes of the WT cells upon treatment. No clear differences in capsule or cell size were observed in the absence of <italic>PKA1</italic> expression using the <italic>pka1</italic>Δ mutant strain and the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain grown under the repressed condition. These findings are consistent with the previously observed changes in cell size upon modulation of <italic>PKA1</italic> expression (<xref rid="B28" ref-type="bibr">28</xref>). We also grew the WT strain, the <italic>pkr1</italic>Δ mutant, and the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain in the presence of a range of bortezomib concentrations to identify the levels that were inhibitory for capsule formation (<xref ref-type="fig" rid="fig4">Fig. 4C</xref>). We found that capsule thickness was first noticeably impacted at 10 µM bortezomib for the WT strain grown under both glucose and galactose capsule-inducing conditions. Conversely, capsule thickness and cell size remained unchanged for the <italic>pkr1</italic>Δ mutant and the induced <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain with a 10 µM concentration of the inhibitor. A reduction in capsule and cell size was noted for these strains once the concentration of inhibitor reached 15 to 20 µM, and a concentration of 25 µM completely eliminated capsule production. Overall, these results are compatible with the conclusion that the regulatory connection between the cAMP/PKA signaling pathway and capsule formation includes a role for the UPP.</p><fig id="fig4" orientation="portrait" position="float"><label>FIG 4 </label><caption><p>Inhibitors of the proteasome or of translation or glycosylation reduce capsule formation. Capsule diameter was examined with differential interference contrast (DIC) microscopy and India ink staining. (A) Capsule formation in the WT strain and the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain grown in low-iron medium (LIM) {either with glucose [LIM (D)] or with galactose [LIM (G)]} in the absence or presence of bortezomib (BTZ; 50 µM). (B) Capsule formation in the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants in LIM (D) in the absence or presence of bortezomib (50 µM). (C) Capsule formation was examined in the WT strain, the <italic>pkr1</italic>Δ mutant, and the induced <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain grown in LIM (D) or LIM (G) in the absence or presence of a range of bortezomib concentrations: 0, 1, 5, 10, 15, 20, and 25 µM. Cells were incubated at 30°C for 48 h, mixed with India ink, and examined by DIC microscopy to visualize the capsule. (D) The WT strain was grown in LIM with or without 50 µM bortezomib (New England Biolabs) and cycloheximide (CHX; 1 µg/ml) for the times indicated. (E) The WT strain was grown in defined LIM in the absence or presence of tunicamycin (TM; 0.1 µg/ml) after incubation for 24 h at 30°C. The bar indicates 10 µm (×100 magnification).</p></caption><graphic xlink:href="mbo0061525880004"></graphic></fig><p>It is interesting that proteasome inhibition reduced capsule formation whereas PKA activation causes an enlarged capsule. These results suggest that a balance in the levels of expression of the UPP must be achieved by regulated PKA activity, perhaps in conjunction with regulation of translation, to properly orchestrate capsule production. To explore this possibility, we tested the translation inhibitor cycloheximide for an influence on capsule formation and found that although cell growth and capsule formation were inhibited, the cells eventually recovered their ability to make capsule (<xref ref-type="fig" rid="fig4">Fig. 4D</xref>). Interestingly, the observed recovery of capsule formation occurred even in the presence of a concentration of bortezomib that otherwise prevented capsule formation. Although additional biochemical studies are needed, these observations support the conclusion that a balance between protein synthesis and degradation has an impact on capsule formation.</p></sec><sec id="s1.6"><title>The proteasome inhibitor bortezomib influences the growth of <italic>C. neoformans</italic> strains with altered PKA expression or activity.</title><p>We next determined the influence of bortezomib on the growth of the WT and the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains, and on the growth of the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants, in liquid media in the presence or absence of exogenous cAMP. Initially, we observed that the regulated <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain showed slower growth than the WT strain in galactose medium without bortezomib but that it eventually reached a similar culture density by 72 h (<xref ref-type="fig" rid="fig5">Fig. 5A</xref>). This result suggests that the activation of <italic>PKA1</italic> expression negatively influences growth, possibly because of the reduction in the abundance of translation functions observed in the proteome analysis (<xref ref-type="table" rid="tab1">Table 1</xref>). The addition of bortezomib negatively impacted the growth of both the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> and WT strains, although substantial growth still occurred. In contrast, addition of both bortezomib and cAMP completely blocked the growth of both strains. These results indicated that activation of PKA by addition of cAMP enhanced susceptibility to bortezomib even in the regulated strain upon galactose-induced expression of <italic>PKA1</italic>. Similar results were observed when the two strains were grown with glucose as the carbon source (<xref ref-type="fig" rid="fig5">Fig. 5B</xref>). The two strains had the same growth profile in the absence of bortezomib (with or without cAMP), but addition of the drug again extended the lag phase and allowed eventual growth at the level seen with the untreated cultures. The inclusion of cAMP with bortezomib again eliminated the growth of both strains. Addition of cAMP alone did not influence the growth of any of the strains (data not shown).</p><fig id="fig5" orientation="portrait" position="float"><label>FIG 5 </label><caption><p>The proteasome inhibitor bortezomib impairs the growth of strains with altered PKA expression or activity. Growth assays were performed for the WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains (A and B) and the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants (C and D) in liquid minimal medium with galactose (MM + G) (A and C) or glucose (MM + D) (B and D) at 37°C. Where indicated, bortezomib was added at 50 µM and cAMP was added at 5 mM. Each experiment was performed a minimum of three times in triplicate. The averages and standard errors of data from an experiment representative of three replicates are shown for each time point.</p></caption><graphic xlink:href="mbo0061525880005"></graphic></fig><p>The influence of bortezomib was examined further by comparing the growth of a <italic>pka1Δ</italic> mutant lacking the main catalytic subunit to that of a <italic>pkr1Δ</italic> mutant lacking the regulatory (cAMP-binding) subunit of PKA (<xref ref-type="fig" rid="fig5">Fig. 5C</xref> and <xref ref-type="fig" rid="fig5">D</xref>). The levels of growth of these strains were similar on galactose (<xref ref-type="fig" rid="fig5">Fig. 5C</xref>) and glucose (<xref ref-type="fig" rid="fig5">Fig. 5D</xref>) without additions, but treatment with bortezomib alone or in combination with cAMP almost completely eliminated the growth of the <italic>pkr1Δ</italic> strain on either carbon source. In contrast, the <italic>pka1Δ</italic> strain in galactose or glucose media showed delayed growth on bortezomib, and the inclusion of cAMP caused a further delay. We note that the Pkr1 regulatory subunit and a second catalytic subunit, Pka2, are still present in the <italic>pka1Δ</italic> mutant. The responsiveness of the mutant may therefore indicate a contribution of Pka2 to the observed response to cAMP and bortezomib. Overall, these experiments demonstrated that bortezomib impairs the growth of <italic>C. neoformans</italic> and that activation of PKA by addition of exogenous cAMP or loss of the regulatory subunit of PKA increases susceptibility. In general, the regulated <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain may not be fully activated for PKA in galactose or fully repressed by glucose because addition of cAMP caused further growth inhibition by bortezomib. Additionally, this strain showed poorer growth with bortezomib in galactose medium versus glucose medium, suggesting that activation of <italic>PKA1</italic> expression increases susceptibility.</p></sec><sec id="s1.7"><title>ER stress caused by tunicamycin impacts the growth of <italic>C. neoformans</italic> strains with altered PKA expression or activity.</title><p>We next hypothesized that the observed influence of PKA on the abundance of proteins for translation and the UPP might be part of a larger impact of PKA activity on proteostasis and protein trafficking. We tested this theory by examining susceptibility to tunicamycin, an antibiotic that provokes ER stress by inhibiting the first step in the lipid-linked oligosaccharide pathway (<xref rid="B40" ref-type="bibr">40</xref>). Specifically, we tested the WT and the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains, as well as the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants, in liquid media in the presence and absence of exogenous cAMP. Initially, we found that the regulated <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain again showed slower growth than the WT strain on galactose medium (<xref ref-type="fig" rid="fig6">Fig. 6A</xref>) as shown in <xref ref-type="fig" rid="fig5">Fig. 5A</xref>. The influence of growth on galactose appeared more pronounced in this experiment, perhaps due to the lower growth temperature (30°C) that was employed. The lower temperature was used because we noted that tunicamycin had a more pronounced influence on susceptibility at 30°C whereas the impact of bortezomib was greater at 37°C. Tunicamycin impaired the growth of both the WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains on galactose, and addition of cAMP did not have a notable influence, although the growth of the strains was already minimal (<xref ref-type="fig" rid="fig6">Fig. 6A</xref>).</p><fig id="fig6" orientation="portrait" position="float"><label>FIG 6 </label><caption><p>The glycosylation inhibitor tunicamycin impairs the growth of strains with altered PKA expression or activity. Growth assays were performed for the WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains (A and B) and the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants (C and D) in liquid minimal medium with galactose (MM + G) (A and C) or glucose (MM + D) (B and D) at 30°C. Where indicated, tunicamycin was added at 0.5 µg/ml and cAMP was added at 1 mM. Each experiment was performed a minimum of three times in triplicate. The averages and standard errors of data from an experiment representative of three replicates are shown for each time point.</p></caption><graphic xlink:href="mbo0061525880006"></graphic></fig><p>The WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains grew to similar levels on glucose, and addition of tunicamycin (with or without cAMP) had only a slight negative impact on the growth of the regulated <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain but severely inhibited the WT strain (<xref ref-type="fig" rid="fig6">Fig. 6B</xref>). That is, a reduction in the expression of <italic>PKA1</italic> under conditions of growth of the regulated strain in glucose eliminated the susceptibility to tunicamycin, suggesting that a low level of Pka1, perhaps resulting in low PKA activity, abrogated the ER stress provoked by the drug. In contrast, the growth of the WT strain was markedly impaired by addition of tunicamycin with or without cAMP. An influence of exogenous cAMP was not observed in this experiment, compared to similar treatment with bortezomib, and this may be because the growth of the WT strain was already quite impaired by treatment with tunicamycin. Importantly, a comparison of the levels of growth of the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain on galactose (<xref ref-type="fig" rid="fig6">Fig. 6A</xref>) versus glucose (<xref ref-type="fig" rid="fig6">Fig. 6B</xref>) revealed a much greater inhibitory influence of tunicamycin under the inducing condition with galactose. We also observed that tunicamycin treatment reduced capsule elaboration, as also seen with bortezomib and cycloheximide (<xref ref-type="fig" rid="fig4">Fig. 4E</xref>).</p><p>We also tested the growth of the <italic>pka1Δ</italic> and <italic>pkr1Δ</italic> mutants with or without tunicamycin and exogenous cAMP (<xref ref-type="fig" rid="fig6">Fig. 6C</xref> and <xref ref-type="fig" rid="fig6">D</xref>). Most notably, the <italic>pkr1Δ</italic> mutant was quite susceptible to tunicamycin, with similar levels of impairment seen with and without cAMP on galactose or glucose medium. The <italic>pka1Δ</italic> strain showed slightly impaired growth in the presence of tunicamycin, and addition of cAMP actually improved the growth of the strain on galactose but not on glucose (compare <xref ref-type="fig" rid="fig6">Fig. 6C</xref> to <xref ref-type="fig" rid="fig6">D</xref>). This result could again indicate a potential role for Pka2 and/or an influence of Pkr1. It was noteworthy that added cAMP had little influence on the susceptibility of the <italic>pka1Δ</italic> mutant compared with its influence on bortezomib susceptibility for this strain, while the <italic>pkr1Δ</italic> mutant was highly susceptible, as seen with bortezomib. In general, a reduction in PKA activity, in either the <italic>pka1Δ</italic> mutant or the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain grown on glucose, protected the strains against ER stress provoked by tunicamycin. These results are consistent with the observed high susceptibility of the <italic>pkr1Δ</italic> mutant to tunicamycin. Taken together, our results revealed that connections between ER stress caused by tunicamycin and the cAMP/PKA pathway exist through the influence of the Pkr1 regulatory subunit on PKA activity or as a consequence of an additional function of Pkr1 and through the expression or activity of the Pka1 protein. Finally, we tested whether there was synergy between tunicamycin and bortezomib by using checkerboard assays to examine drug susceptibility (<xref rid="B41" ref-type="bibr">41</xref>). We did not observe synergy for these drugs or for bortezomib and fluconazole, an antifungal drug known to provoke ER stress (42 and data not shown).</p></sec><sec id="s1.8"><title>Phenotypes of deletion mutants encoding PKA-regulated proteins.</title><p>We compared our list of Pka1-regulated proteins with the <italic>C. neoformans</italic> gene deletion set to gain a more in-depth appreciation of the impact of <italic>PKA1</italic> modulation on the proteome (<xref rid="B43" ref-type="bibr">43</xref>, <xref rid="B44" ref-type="bibr">44</xref>). In total, we linked our Pka1-regulated proteins with 59 deletion mutants that had previously been assessed for the production of virulence factors and growth at 37°C and that had been recently tested by chemical genetic analysis for sensitivity or resistance to small molecules (see <xref ref-type="supplementary-material" rid="tabS5">Table S5</xref> in the supplemental material) (<xref rid="B43" ref-type="bibr">43</xref>, <xref rid="B44" ref-type="bibr">44</xref>). Of these 59 genes, the mutants encoded by 3 displayed a defect in melanin production, and the corresponding genes encoded a ubiquitin-like protein (CNAG_02827), a class E vacuolar protein-sorting machinery protein, HSE1 (CNAG_05882), and an uncharacterized protein (CNAG_01644) (<xref rid="B43" ref-type="bibr">43</xref>). These results are consistent with the known regulation of melanin production by PKA (<xref rid="B19" ref-type="bibr">19</xref>). Twenty-nine deletion mutants showed growth phenotypes, including sensitivity to H<sub>2</sub>O<sub>2</sub>, FeCl<sub>3</sub>, sirolimus, and amphotericin B, upon challenge by small molecules. Several deletion strains displayed sensitivity to cell wall stressors, including SDS, Congo red, and caffeine, suggesting a role in cell wall integrity and potential capsule attachment. Mutants for several genes showed resistance to H<sub>2</sub>O<sub>2</sub> or azole drugs such as fluconazole, suggesting roles for the proteins in protection against oxidative stress or ergosterol biosynthesis. These observations are interesting given the impact of redox activity on disulfide bond formation in the ER. Interestingly, three mutants showed an impact of ER stress triggered by tunicamycin. These mutants had defects in a translation-associated argonaute protein (CNAG_04609) or a glycosyl hydrolase (CNAG_05077) that enhanced susceptibility and in a phosphoketolase (CNAG_02230) that led to resistance. These observations further support the idea of a connection between PKA and ER function as well as of other potential links between PKA regulation and specific proteins that warrant further study.</p></sec></sec><sec sec-type="discussion" id="h2"><title>DISCUSSION</title><p>The cAMP/PKA pathway plays a critical role in the virulence of <italic>C. neoformans</italic> because it regulates capsule and melanin formation (<xref rid="B16" ref-type="bibr">16</xref>, <xref rid="B17" ref-type="bibr">17</xref>, <xref rid="B19" ref-type="bibr">19</xref>). In this study, we used a quantitative proteomics approach to investigate the influence of <italic>PKA1</italic> expression on the proteome, and we identified 48.1% of the 6,692 predicted proteins from the genome sequence, including 1,453 and 1,435 under Pka1-repressed and Pka1-induced conditions, respectively. This level of protein identification is consistent with other proteome studies, including a recent examination of biofilm formation in <italic>C. neoformans</italic> (<xref rid="B45" ref-type="bibr">45</xref>). Remarkably, the major pattern of regulation observed upon induction of <italic>PKA1</italic> expression was a decrease in abundance for ribosomal and translational proteins, proteins associated with the UPP, and proteins associated with metabolism and biosynthesis. This regulatory pattern reflects an impact on proteostasis, and we found that strains with elevated PKA activity or expression were more susceptible to the anticancer drug and proteasome inhibitor bortezomib than the WT strain or strains with low PKA activity. These results are consistent with the identification of a PKA mutant in a screen for mutations causing synthetic lethality with bortezomib in <italic>Schizosaccharomyces pombe</italic> (<xref rid="B46" ref-type="bibr">46</xref>).</p><sec id="s2.1"><title>The impact of Pka1 regulation on translation.</title><p>A key observation from this study was that induction of Pka1 suppressed the abundance of components of the translational machinery. Our previous transcript profiling of <italic>pka1</italic> and <italic>pkr1</italic> deletion mutants in <italic>C. neoformans</italic> also revealed a connection between Pka1 and expression of the translational machinery (<xref rid="B16" ref-type="bibr">16</xref>). We note, however, that other comparisons between our observed changes in protein abundance and transcript levels did not always reveal a correlation, perhaps because of differences in growth conditions and the strains (i.e., the <italic>PKA1</italic>-regulated strain versus the <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants). The idea of the significance of the connection between cAMP/PKA signaling and translation in <italic>C. neoformans</italic> was recently bolstered by the characterization of Gib2, a protein that regulates cAMP levels via interaction with Ras1 and adenylyl cyclase (Cac1) and that is part of an interaction network with ribosomal proteins (<xref rid="B47" ref-type="bibr">47</xref>). The relationship between PKA and translation is interesting in the context of interactions between fungal pathogens and the phagocytic cells of vertebrate hosts. It is known, for example, that the phagocytosis of <italic>C. neoformans</italic> and <italic>Candida albicans</italic> by mammalian macrophages results in downregulation of transcripts for the translational machinery (<xref rid="B48" ref-type="bibr">48</xref>, <xref rid="B49" ref-type="bibr">49</xref>). More broadly, an influence of PKA on the expression of proteins involved in translation occurs in a number of fungi, including <italic>C. albicans</italic>, <italic>Ustilago maydis</italic>, and <italic>Saccharomyces cerevisiae</italic> (<xref rid="B50" ref-type="bibr">50</xref><xref ref-type="bibr" rid="B51">–</xref><xref rid="B54" ref-type="bibr">54</xref>). This regulation is best understood in <italic>S. cerevisiae</italic>, where nutritional signals influence the transcription of rRNA, as well as of genes for ribosome biogenesis and ribosomal proteins, via transcription factors controlled by the cAMP/PKA and target of rapamycin (TOR) pathways (<xref rid="B55" ref-type="bibr">55</xref>). Extensive studies in <italic>S. cerevisiae</italic> document PKA regulation of the translation machinery during the transient response to glucose as well as the unfolded protein response, the environmental stress response, autophagy, and yeast versus pseudohyphal/filamentous growth (<xref rid="B50" ref-type="bibr">50</xref>, <xref rid="B54" ref-type="bibr">54</xref>, <xref rid="B55" ref-type="bibr">55</xref><xref ref-type="bibr" rid="B56">–</xref><xref rid="B58" ref-type="bibr">58</xref>). The latter process may reflect a role for PKA on a longer time scale than that associated with its participation in the transient response to glucose (<xref rid="B55" ref-type="bibr">55</xref>). These other influences of PKA in <italic>S. cerevisiae</italic> may therefore be more relevant to the sustained activation expected from induction of <italic>PKA1</italic> expression in our experiments with <italic>C. neoformans</italic>. In particular, autophagy and the response to stress warrant further study.</p></sec><sec id="s2.2"><title>Connections between PKA, translation, the UPP, and ER stress.</title><p>Our observation of an influence of Pka1 expression on the abundance of UPP components is similar to an observed connection between PKA and proteostasis found in chronic neurodegenerative disorders and other diseases in humans. In this context, the UPP is a potential pharmacological target for the prevention and treatment of Alzheimer disease (AD), Parkinson disease (PD), Huntington disease (HD), and amyotropic lateral sclerosis (ALS), as well as cardiovascular conditions such as hypertrophic and dilated cardiomyopathies and ischemic heart disease (<xref rid="B30" ref-type="bibr">30</xref>, <xref rid="B31" ref-type="bibr">31</xref>, <xref rid="B59" ref-type="bibr">59</xref>). All of these conditions are associated with impaired protein turnover and the accumulation of intracellular ubiquitin-protein aggregates (<xref rid="B30" ref-type="bibr">30</xref>, <xref rid="B31" ref-type="bibr">31</xref>). For example, proteasome impairment is associated with lower PKA activity leading to progression of HD, and regulation based on positive feedback between PKA and the proteasome appears to be critical for HD pathogenesis (<xref rid="B60" ref-type="bibr">60</xref>, <xref rid="B61" ref-type="bibr">61</xref>). Ribosomes also play important roles in cotranslational ubiquitination and quality control since newly synthesized polypeptides must be properly folded to avoid aggregation (<xref rid="B60" ref-type="bibr">60</xref>, <xref rid="B61" ref-type="bibr">61</xref>). Specifically, a tiered system of quality control at the ribosome is responsible for proteostasis during protein synthesis that operates by sensing the nature of nascent protein chains, recruiting protein folding and translocation components, and integrating mRNA and nascent-chain quality control (<xref rid="B61" ref-type="bibr">61</xref>). In this regard, we observed a reduction in the abundance of a number of chaperones (e.g., GrpE), proteins for sorting (e.g., prefoldin), a peptidyl-prolyl <italic>cis</italic>-<italic>trans</italic> isomerase, a subunit of the signal recognition particle receptor, and proteins for RNA processing. These observations suggest that a key role of Pka1 in <italic>C. neoformans</italic> is to regulate translation as well as functions for protein folding/sorting and the UPP machinery for degradation of misfolded proteins.</p><p>Given the observations described above, we hypothesize that the elevated Pka1 activity that occurs upon galactose induction of <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> expression may invoke ER stress and the unfolded protein response and, as a consequence, may change the requirements for proteasome activity and other processes related to proteostasis (<xref rid="B62" ref-type="bibr">62</xref>, <xref rid="B63" ref-type="bibr">63</xref>). PKA activation in <italic>C. neoformans</italic> appears to suppress the abundance of proteins for these processes, perhaps as a mechanism to favor specific functions for the export of a large amount of capsule polysaccharide to the cell surface. It is curious, however, that galactose activation of <italic>PKA1</italic> expression in the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain results in a large capsule and reduces the abundance of UPP proteins but inhibition of UPP activity with bortezomib impairs capsule formation. These observations suggest that a balanced regulation of PKA activity (and/or expression of <italic>PKA1</italic>) is needed for proper capsule production. The observation that cycloheximide also inhibits capsule formation but can allow production of a smaller capsule in the presence of bortezomib is consistent with the requirement for a balance. Moreover, the levels of free ubiquitin in the cell may be a component of the balance because studies in <italic>S. cerevisiae</italic> reveal that cycloheximide and proteasome inhibitors deplete ubiquitin by reducing translation and by promoting conjugation through the accumulation of substrates, respectively (<xref rid="B64" ref-type="bibr">64</xref>). Support for the idea of a connection with ubiquitin in <italic>C. neoformans</italic> comes from the observation that the effect of bortezomib treatment mimics the effect of a defect in the E3 ligase Fbp1 with regard to membrane integrity (<xref rid="B65" ref-type="bibr">65</xref>).</p><p>Our experiments with tunicamycin and bortezomib revealed an impact of both ER stress and proteasome inhibition on the growth of <italic>C. neoformans</italic> strains with altered PKA activity or expression. This was particularly prominent for the <italic>pkr1</italic>Δ mutant, which is expected to have high PKA activity due to loss of the regulatory subunit of the enzyme. Both tunicamycin and bortezomib effectively shut down the growth of this mutant at concentrations that allowed growth of the WT strain. However, the addition of exogenous cAMP to ensure activation of PKA in the strains revealed two key differences with regard to susceptibility to bortezomib versus tunicamycin. First, the addition of cAMP to cultures of the WT or the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains completely inhibited growth in the presence of bortezomib on media with galactose or glucose. Presumably, these conditions resulted in high PKA activity in the WT strain, leading to increased bortezomib susceptibility. The impact of cAMP on the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain may reflect partial or eventual relief from glucose repression, leading to <italic>PKA1</italic> expression and/or potential activation of the second catalytic subunit, Pka2. In contrast, the <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strain on glucose (with or without cAMP) showed greater resistance to tunicamycin than the WT strain (compare <xref ref-type="fig" rid="fig5">Fig. 5B</xref> to <xref ref-type="fig" rid="fig6">6B</xref>). This result may indicate an alternative mechanism in which reduction in the level of Pka1 protein is the key factor in determining susceptibility to tunicamycin. Second, the addition of cAMP increased the susceptibility of the <italic>pka1</italic>Δ mutant to bortezomib on galactose and glucose while reducing and not influencing susceptibility to tunicamycin on galactose and glucose, respectively. In the latter case, it is possible that addition of cAMP increased the activity of Pka2 in the <italic>pka1</italic>Δ mutant and caused increased susceptibility to bortezomib. The contributions of the Pka2 subunit are currently not well understood, although it was previous found that it did not contribute to virulence-related phenotypes (<xref rid="B19" ref-type="bibr">19</xref>, <xref rid="B25" ref-type="bibr">25</xref>).</p><p>The inhibitor experiments also suggest that induction of <italic>PKA1</italic> expression in the regulated strain by galactose may not completely override the influence of Pkr1 and the requirement for elevated cAMP levels for full activation. Additionally, a clear distinction must be made between the influence of overexpressing Pka1 by galactose induction and activation of cAMP signaling leading to higher PKA activity. Pka1 overexpression might sequester Pkr1 and change its influence on downstream targets. We previously observed an increase in <italic>PKR1</italic> transcript levels upon galactose induction of <italic>PKA1</italic> expression, thus indicating further levels of regulation (<xref rid="B16" ref-type="bibr">16</xref>, <xref rid="B28" ref-type="bibr">28</xref>). It is also possible that the Pka1 or Pkr1 proteins themselves influence ER function, as well as capsule synthesis and trafficking, and these may be independent of PKA activity. Together, these observations may explain phenotypic dissimilarities between the regulated strain and the <italic>pkr1Δ</italic> mutant beyond possible differences in the extent or duration of PKA activation in the two strains.</p><p>Bortezomib is known to display synergy with other chemotherapeutic agents, and it has been used to restore sensitivity to chemotherapy for resistant cancer cells (<xref rid="B32" ref-type="bibr">32</xref>, <xref rid="B66" ref-type="bibr">66</xref>). In this context, it is interesting that a synergistic interaction of bortezomib with fluconazole was previously reported for <italic>C. albicans</italic> (<xref rid="B67" ref-type="bibr">67</xref>). Although we did not observe similar synergy for <italic>C. neoformans</italic>, the effect of bortezomib on growth and capsule formation suggests that further investigation of combinations with other drugs is warranted. We also did not observe synergy between bortezomib and tunicamycin, although both of these drugs interact with PKA. The impact of tunicamycin on capsule formation suggests that it may be fruitful to test combinations of agents that provoke ER stress and modulators of cAMP signaling such as cAMP derivatives and phosphodiesterase inhibitors. In particular, ER function and aspects of ER stress may be useful targets for antifungal drug development given recent associations with virulence and drug susceptibility in <italic>Aspergillus fumigatus</italic> and <italic>Candida glabrata</italic> (<xref rid="B68" ref-type="bibr">68</xref>, <xref rid="B69" ref-type="bibr">69</xref>). More generally, our proteome analysis, in combination with the inhibitor studies, revealed that proteostasis is a promising therapeutic target for the treatment of cryptococcosis.</p></sec></sec><sec sec-type="materials|methods" id="h3"><title>MATERIALS AND METHODS</title><sec id="s3.1"><title>Fungal strains and culture conditions.</title><p><italic>Cryptococcus neoformans</italic> var. <italic>grubii</italic> wild-type strain H99 (WT), H99 <italic>pka1</italic>Δ and <italic>pkr1</italic>Δ mutants, and galactose-inducible <italic>PKA1</italic> strain <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> (<xref rid="B19" ref-type="bibr">19</xref>, <xref rid="B28" ref-type="bibr">28</xref>) were used for the analyses. For regulation of <italic>PKA1</italic> expression, cells were grown in minimal medium (MM) containing either 0.27% glucose (MM + D) or 0.27% galactose (MM + G).</p></sec><sec id="s3.2"><title>Preparation of protein extracts and peptides.</title><p>Cellular fractions were processed in triplicate for total protein extraction as previously described (<xref rid="B70" ref-type="bibr">70</xref>). Protein concentration was determined using the BCA protein assay (Pierce), and an in-solution trypsin digestion was performed on the total cellular extracts. Digested peptides from cellular fractions were subjected to C18 STop and Go Extraction (STAGE) tips (<xref rid="B71" ref-type="bibr">71</xref>). Reductive dimethylation was performed for differential peptide labeling (<xref rid="B72" ref-type="bibr">72</xref>). Digested and purified peptides were further fractioned by strong cation exchange (SCX) chromatography.</p></sec><sec id="s3.3"><title>Protein identification by LC-tandem MS (LC-MS/MS) and MS data analysis.</title><p>Purified and SCX-fractionated peptides were analyzed using a linear-trapping quadrupole–orbitrap mass spectrometer (LTQ-Orbitrap Velos; Thermo Fisher Scientific) online coupled to an Agilent 1290 Series high-performance liquid chromatography (HPLC) system using a nanospray ionization source (Thermo Fisher Scientific). MaxQuant 1.3.0.25 was used for the analysis and quantification of mass spectrometry data, with statistical analysis and data visualization performed using Perseus 1/3/09 (<xref rid="B73" ref-type="bibr">73</xref><xref ref-type="bibr" rid="B74">–</xref><xref rid="B75" ref-type="bibr">75</xref>). The search was performed against a database comprised of 6,692 protein sequences from the <italic>C. neoformans</italic> WT source organism (<italic>Cryptococcus neoformans</italic> var. <italic>grubii</italic> H99; <ext-link ext-link-type="uri" xlink:href="http://www.uniprot.org">http://www.uniprot.org</ext-link>). Data corresponding to experimentally determined fold changes for WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains grown under conditions of Pka1 repression (glucose-containing medium) and Pka1 induction (galactose-containing medium) were normalized and converted to a log<sub>2</sub> scale, and the average fold change values and standard deviations were used for analysis. A Student’s <italic>t</italic> test (<italic>P</italic> value, <0.05) was performed for cellular proteins identified under both Pka1-repressed and Pka1-induced conditions to evaluate the statistical significance of the data. Multiple-hypothesis testing correction was performed on the proteome data using the Benjamini and Hochberg method (false-discovery rate [FDR], <0.05) (<xref rid="B76" ref-type="bibr">76</xref>). Proteins were characterized with Gene Ontology (GO) terms using a local installation of Blast2GO (<xref rid="B77" ref-type="bibr">77</xref>). Of the 302 Pka1-regulated proteins identified, 300 proteins could be mapped to the <italic>C. neoformans</italic> JEC21 database for data analysis and visualization (<xref rid="B78" ref-type="bibr">78</xref>). STRING (Search Tool for Retrieval of Interacting Genes/Proteins; <ext-link ext-link-type="uri" xlink:href="http://string-db.org">http://string-db.org</ext-link>) was used to visualize predicted protein-protein interactions for the 300 identified Pka1-regulated proteins (<xref rid="B79" ref-type="bibr">79</xref>). The mass spectrometry (MS) proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with data set identifier PXD002727 (<xref rid="B80" ref-type="bibr">80</xref>).</p></sec><sec id="s3.4"><title>Enzyme and inhibitor assays and immunoblot analysis.</title><p>Enzymatic assays were performed in triplicate according to the protocol of the manufacturer (BioVision Inc.). Hsp70 was detected by immunoblot analysis using a monoclonal anti-mouse antibody (Thermo Scientific Pierce; 1:3,000 dilution) followed by enhanced chemiluminescence (ECL; Amersham) for visualization. The impact of bortezomib (BTZ), cycloheximide (CHX), and tunicamycin on capsule elaboration was investigated using visualization with India ink. The WT, <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic>, <italic>pka1</italic>Δ, and <italic>pkr1</italic>Δ strains were grown overnight in 5 ml of yeast extract-peptone-dextrose (YPD) at 30°C. Subsequently, 1 × 10<sup>5</sup> cells/ml were inoculated into 200 µl of low-iron medium (LIM) with glucose [LIM (D)] or LIM with galactose [LIM (G)] with or without 50 µM bortezomib (New England Biolabs) and/or 1 µg/ml of cycloheximide. Cultures were incubated at 37°C for up to 72 h. For tunicamycin, 1 × 10<sup>5</sup> cells/ml were inoculated in 5 ml of low-iron medium (LIM glucose with or without 0.1 µg/ml of the drug) and incubated at 30°C for 24 h. LIM was prepared with 20 mM HEPES and 22 mM NaHCO<sub>3</sub> and glucose or galactose as the carbon source for the respective strains as previously described (<xref rid="B16" ref-type="bibr">16</xref>). After incubation, the capsule was visualized with India ink and examined by differential interference microscopy (DIC). A titration of bortezomib was also used to assess the lowest concentration capable of influencing capsule production. The WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains and the <italic>pkr1</italic>Δ mutant were grown overnight at 30°C in 5 ml of YPD, and 1 × 10<sup>5</sup> cells were then used to inoculate 200 µl of LIM (D) or LIM (G) with or without bortezomib (1 to 25 µM). The cells were incubated at 30°C for 48 h, and the capsule was visualized with India ink using differential interference microscopy (DIC). All assays were performed a minimum of three times.</p><p>The influence of proteasome inhibition and ER stress on the growth of the WT, <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic>, <italic>pka1</italic>Δ, and <italic>pkr1</italic>Δ strains was tested by adding bortezomib and tunicamycin, respectively, to growth medium. Briefly, strains were grown overnight at 30°C in 5 ml of YPD and the cells from 50 µl of the overnight cultures were then washed and transferred to 5 ml of MM + D or MM + G and grown overnight at 30°C. Following the initial incubation, 1 × 10<sup>5</sup> cells/ml were used to inoculate 12 ml of MM + D or MM + G with or without 50 µM bortezomib or 0.5 µg/ml of tunicamycin. Growth assays were performed in triplicate at 37°C for bortezomib and 30°C for tunicamycin with optical density measurements at 600 nm (OD<sub>600</sub>) every 24 h. Where indicated, adenosine 3′,5′-cyclic monophosphate (cAMP; Sigma) was added to the growth medium at concentrations of 5 mM for the bortezomib assays and 1 mM for the tunicamycin assays. All assays were performed a minimum of three times in triplicate. Potential synergy between drugs was assessed by a checkerboard assay based on standardized methods proposed by the National Committee for Clinical Laboratory Standards (NCCLS) for broth microdilution antifungal susceptibility testing and as previously described (<xref rid="B41" ref-type="bibr">41</xref>, <xref rid="B81" ref-type="bibr">81</xref>).</p><p>Additional details of the materials and methods used are available in the supplemental material.</p></sec></sec><sec sec-type="supplementary-material" id="sm1"><title>SUPPLEMENTAL MATERIAL</title><supplementary-material id="textS1"><label>Text S1 </label><caption><p>Supplemental methods. Download </p></caption><media xlink:href="mbo006152588s1.docx"><caption><p>Text S1, DOCX file, 0.01 MB</p></caption></media></supplementary-material><supplementary-material id="figS1"><label>Figure S1 </label><caption><p>Enrichment of genes represented in the proteome analysis upon modulation of <italic>PKA1</italic> expression compared to all genes present in the genome. (A to C) The enrichment was based on GO terms associated with biological processes (A), cellular components (B), and molecular functions (C). (D) Additionally, enrichment of Pka1-regulated genes was determined based on the biological classifications represented in the proteome analysis. Strains were grown under Pka1-repressed conditions (glucose-containing medium) or Pka1-induced conditions (galactose-containing medium). Statistical analysis of the data set was performed using the Benjamini and Hochberg false-discovery-rate multiple-testing correction (<italic>P</italic> value, <0.05). Download </p></caption><media xlink:href="mbo006152588sf1.pdf"><caption><p>Figure S1, PDF file, 0.3 MB</p></caption></media></supplementary-material><supplementary-material id="figS2"><label>Figure S2 </label><caption><p>Validation of proteomic profiling. Enzyme activities were measured for phosphatase (A) and superoxide dismutase (SOD) (B) in the WT and <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> strains under Pka1-repressed conditions (glucose-containing medium [D]) and Pka1-induced conditions (galactose-containing medium [G]). Values are reported as averages ± standard deviations (SD) of the results from three independent replicates. Statistical analysis was performed using a Student’s <italic>t</italic> test (<italic>P</italic> value, <0.05). An asterisk (*) denotes a significant difference between the results seen under the indicated conditions. (C) An immunoblot was performed for Hsp70 to validate the proteomic results. Cellular proteins were extracted from <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> cells grown under conditions of Pka1 repression (glucose-containing medium [D]) and Pka1 induction (galactose-containing medium [G]) for 16 h. β-tubulin was used as a control, and Hsp70 protein abundance was assessed under the different growth conditions. The results show a decrease in the abundance of Hsp70 under the Pka1-induced conditions. Download </p></caption><media xlink:href="mbo006152588sf2.pdf"><caption><p>Figure S2, PDF file, 0.2 MB</p></caption></media></supplementary-material><supplementary-material id="figS3"><label>Figure S3 </label><caption><p>RNA expression levels for the <italic>PKA1</italic> strain. (A) Expression levels at 16 hpi were measured for the <italic>PKA1</italic> strain by quantitative reverse transcription-PCR (qRT-PCR) in <italic>P</italic><sub>GAL7</sub>::<italic>PKA1</italic> and WT strains grown under conditions of Pka1 repression (glucose-containing medium) and Pka1 induction (galactose-containing medium). Samples were evaluated in triplicate, and the values are reported as average log<sub>2</sub> fold change ± standard deviation. Statistical significance was determined using a Student’s <italic>t</italic> test (<italic>P</italic> value, <0.05), and the line denotes a significant difference between the conditions. (B) Northern blot analysis was performed to validate the qRT-PCR results. RNA was extracted from <italic>P</italic><sub>GAL7</sub>::<italic>Pka1</italic> cells grown under conditions of Pka1 repression (glucose-containing medium) and Pka1 induction (galactose-containing medium) for 16 h. Actin was used as a loading control, and <italic>PKA1</italic> expression was assessed under the different growth conditions. The results show an upregulation of <italic>PKA1</italic> mRNA under Pka1-inducting conditions. Download </p></caption><media xlink:href="mbo006152588sf3.pdf"><caption><p>Figure S3, PDF file, 0.2 MB</p></caption></media></supplementary-material><supplementary-material id="tabS1"><label>Table S1 </label><caption><p>Identification of Pka1-regulated proteins by quantitative proteomics in <italic>C. neoformans</italic></p></caption><media xlink:href="mbo006152588st1.pdf"><caption><p>Table S1, PDF file, 0.1 MB</p></caption></media></supplementary-material><supplementary-material id="tabS2"><label>Table S2 </label><caption><p>Enrichment of Pka1-regulated genes compared to all genes present in the WT strain.</p></caption><media xlink:href="mbo006152588st2.pdf"><caption><p>Table S2, PDF file, 0.1 MB</p></caption></media></supplementary-material><supplementary-material id="tabS3"><label>Table S3 </label><caption><p>Detailed KEGG pathways impacted by Pka1-regulated proteins in <italic>C. neoformans</italic></p></caption><media xlink:href="mbo006152588st3.pdf"><caption><p>Table S3, PDF file, 0.1 MB</p></caption></media></supplementary-material><supplementary-material id="tabS4"><label>Table S4 </label><caption><p>Characterization and classification of hypothetical and unclassified proteins in the proteome of <italic>C. neoformans</italic> under conditions of regulation of Pka1.</p></caption><media xlink:href="mbo006152588st4.pdf"><caption><p>Table S4, PDF file, 0.1 MB</p></caption></media></supplementary-material><supplementary-material id="tabS5"><label>Table S5 </label><caption><p>Pka1-regulated proteins from <italic>C. neoformans</italic> present in the ATCC gene deletion set.</p></caption><media xlink:href="mbo006152588st5.pdf"><caption><p>Table S5, PDF file, 0.1 MB</p></caption></media></supplementary-material></sec></body><back><fn-group><fn fn-type="other"><p><bold>Citation</bold> Geddes JMH, Caza M, Croll D, Stoynov N, Foster LJ, Kronstad JW. 2016. Analysis of the protein kinase A-regulated proteome of <italic>Cryptococcus neoformans</italic> identifies a role for the ubiquitin-proteasome pathway in capsule formation. mBio 7(1):e01862-15. doi:10.1128/mBio.01862-15.</p></fn></fn-group><ack><title>ACKNOWLEDGMENTS</title><p>We thank J. Choi for strain construction and G. Hu, M. Kretschmer, S. Michaud, N. Scott, and J. Moon for helpful discussions and technical assistance.</p><p>This work was supported by an <funding-source id="gs1">NSERC</funding-source> fellowship to J.M.H.G., <funding-source id="gs2">CIHR</funding-source><award-id>open operating grants</award-id> to J.W.K. and L.J.F., and a <funding-source id="gs4">Burroughs Wellcome Fund</funding-source> Scholar Award <award-id rid="gs4">in Molecular Pathogenic Mycology</award-id> (J.W.K.). 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