Calpain-Cleavage of α-Synuclein
Identifieur interne : 000C19 ( Pmc/Checkpoint ); précédent : 000C18; suivant : 000C20Calpain-Cleavage of α-Synuclein
Auteurs : Brian M. Dufty ; Lisa R. Warner ; Sheng T. Hou ; Susan X. Jiang ; Teresa Gomez-Isla ; Kristen M. Leenhouts ; Julia T. Oxford ; Mel B. Feany ; Eliezer Masliah ; Troy T. RohnSource :
- The American Journal of Pathology [ 0002-9440 ] ; 2007.
Abstract
Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) are both characterized pathologically by the presence of neuronal inclusions termed Lewy bodies (LBs). A common feature found in LBs are aggregates of α-synuclein (α-Syn), and although it is now recognized that α-Syn is the major building block for these toxic filaments, the mechanism of how this occurs remains unknown. In the present study, we demonstrate that proteolytic processing of α-Syn by the protease calpain I leads to the formation of aggregated high-molecular weight species and adoption of a β-sheet structure. To determine whether calpain-cleavage of α-Syn occurs in PD and DLB, we designed site-directed calpain-cleavage antibodies to α-Syn and tested their utility in several animal model systems. Detection of calpain-cleaved α-Syn was evident in mouse models of cerebral ischemia and PD and in a
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DOI: 10.2353/ajpath.2007.061232
PubMed: 17456777
PubMed Central: 1854966
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Calpain-Cleavage of α-Synuclein </title><author><name sortKey="Dufty, Brian M" sort="Dufty, Brian M" uniqKey="Dufty B" first="Brian M." last="Dufty">Brian M. Dufty</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Warner, Lisa R" sort="Warner, Lisa R" uniqKey="Warner L" first="Lisa R." last="Warner">Lisa R. Warner</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Hou, Sheng T" sort="Hou, Sheng T" uniqKey="Hou S" first="Sheng T." last="Hou">Sheng T. Hou</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Jiang, Susan X" sort="Jiang, Susan X" uniqKey="Jiang S" first="Susan X." last="Jiang">Susan X. Jiang</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Gomez Isla, Teresa" sort="Gomez Isla, Teresa" uniqKey="Gomez Isla T" first="Teresa" last="Gomez-Isla">Teresa Gomez-Isla</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Leenhouts, Kristen M" sort="Leenhouts, Kristen M" uniqKey="Leenhouts K" first="Kristen M." last="Leenhouts">Kristen M. Leenhouts</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Oxford, Julia T" sort="Oxford, Julia T" uniqKey="Oxford J" first="Julia T." last="Oxford">Julia T. Oxford</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Feany, Mel B" sort="Feany, Mel B" uniqKey="Feany M" first="Mel B." last="Feany">Mel B. Feany</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Masliah, Eliezer" sort="Masliah, Eliezer" uniqKey="Masliah E" first="Eliezer" last="Masliah">Eliezer Masliah</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Rohn, Troy T" sort="Rohn, Troy T" uniqKey="Rohn T" first="Troy T." last="Rohn">Troy T. Rohn</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">17456777</idno><idno type="pmc">1854966</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1854966</idno><idno type="RBID">PMC:1854966</idno><idno type="doi">10.2353/ajpath.2007.061232</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">000210</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000210</idno><idno type="wicri:Area/Pmc/Curation">000210</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000210</idno><idno type="wicri:Area/Pmc/Checkpoint">000C19</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000C19</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Calpain-Cleavage of α-Synuclein </title><author><name sortKey="Dufty, Brian M" sort="Dufty, Brian M" uniqKey="Dufty B" first="Brian M." last="Dufty">Brian M. Dufty</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Warner, Lisa R" sort="Warner, Lisa R" uniqKey="Warner L" first="Lisa R." last="Warner">Lisa R. Warner</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Hou, Sheng T" sort="Hou, Sheng T" uniqKey="Hou S" first="Sheng T." last="Hou">Sheng T. Hou</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Jiang, Susan X" sort="Jiang, Susan X" uniqKey="Jiang S" first="Susan X." last="Jiang">Susan X. Jiang</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Gomez Isla, Teresa" sort="Gomez Isla, Teresa" uniqKey="Gomez Isla T" first="Teresa" last="Gomez-Isla">Teresa Gomez-Isla</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Leenhouts, Kristen M" sort="Leenhouts, Kristen M" uniqKey="Leenhouts K" first="Kristen M." last="Leenhouts">Kristen M. Leenhouts</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Oxford, Julia T" sort="Oxford, Julia T" uniqKey="Oxford J" first="Julia T." last="Oxford">Julia T. Oxford</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Feany, Mel B" sort="Feany, Mel B" uniqKey="Feany M" first="Mel B." last="Feany">Mel B. Feany</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Masliah, Eliezer" sort="Masliah, Eliezer" uniqKey="Masliah E" first="Eliezer" last="Masliah">Eliezer Masliah</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author><author><name sortKey="Rohn, Troy T" sort="Rohn, Troy T" uniqKey="Rohn T" first="Troy T." last="Rohn">Troy T. Rohn</name><affiliation><nlm:aff id="N0x2dec680N0x36db630"></nlm:aff></affiliation></author></analytic><series><title level="j">The American Journal of Pathology</title><idno type="ISSN">0002-9440</idno><idno type="eISSN">1525-2191</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) are both characterized pathologically by the presence of neuronal inclusions termed Lewy bodies (LBs). A common feature found in LBs are aggregates of α-synuclein (α-Syn), and although it is now recognized that α-Syn is the major building block for these toxic filaments, the mechanism of how this occurs remains unknown. In the present study, we demonstrate that proteolytic processing of α-Syn by the protease calpain I leads to the formation of aggregated high-molecular weight species and adoption of a β-sheet structure. To determine whether calpain-cleavage of α-Syn occurs in PD and DLB, we designed site-directed calpain-cleavage antibodies to α-Syn and tested their utility in several animal model systems. Detection of calpain-cleaved α-Syn was evident in mouse models of cerebral ischemia and PD and in a <bold><italic>Drosophila</italic></bold> model of PD. In the human PD and DLB brain, calpain-cleaved α-Syn antibodies immunolabeled LBs and neurites in the substantia nigra. Moreover, calpain-cleaved α-Syn fragments identified within LBs colocalized with activated calpain in neurons of the PD and DLB brains. These findings suggest that calpain I may participate in the disease-linked aggregation of α-Syn in various α-synucleinopathies.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Am J Pathol</journal-id><journal-title>The American Journal of Pathology</journal-title><issn pub-type="ppub">0002-9440</issn><issn pub-type="epub">1525-2191</issn><publisher><publisher-name>American Society for Investigative Pathology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">17456777</article-id><article-id pub-id-type="pmc">1854966</article-id><article-id pub-id-type="doi">10.2353/ajpath.2007.061232</article-id><article-categories><subj-group subj-group-type="heading"><subject>Regular Articles</subject><subj-group><subject>Neurobiology</subject></subj-group></subj-group></article-categories><title-group><article-title>Calpain-Cleavage of α-Synuclein </article-title><subtitle>Connecting Proteolytic Processing to Disease-Linked Aggregation</subtitle></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dufty</surname><given-names>Brian M.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">*</xref></contrib><contrib contrib-type="author"><name><surname>Warner</surname><given-names>Lisa R.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">*</xref></contrib><contrib contrib-type="author"><name><surname>Hou</surname><given-names>Sheng T.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">†</xref></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Susan X.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">†</xref></contrib><contrib contrib-type="author"><name><surname>Gomez-Isla</surname><given-names>Teresa</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">‡</xref></contrib><contrib contrib-type="author"><name><surname>Leenhouts</surname><given-names>Kristen M.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">*</xref></contrib><contrib contrib-type="author"><name><surname>Oxford</surname><given-names>Julia T.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">*</xref></contrib><contrib contrib-type="author"><name><surname>Feany</surname><given-names>Mel B.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">§</xref></contrib><contrib contrib-type="author"><name><surname>Masliah</surname><given-names>Eliezer</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">¶</xref></contrib><contrib contrib-type="author"><name><surname>Rohn</surname><given-names>Troy T.</given-names></name><xref ref-type="aff" rid="N0x2dec680N0x36db630">*</xref></contrib><aff id="N0x2dec680N0x36db630">From the Department of Biology,<label>*</label> Boise State University, Boise, Idaho; the Experimental NeuroTherapeutics Laboratory,<label>†</label> National Research Council Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada; the Unidad de Memoria,<label>‡</label> Servicio de Neurologia, Hospital Santa Creu i Sant Pau, Barcelona, Spain; the Department of Pathology,<label>§</label> Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts; and the Department of Neurosciences and Neuropathology,<label>¶</label> University of California, San Diego, La Jolla, California</aff></contrib-group><pub-date pub-type="ppub"><month>5</month><year>2007</year></pub-date><volume>170</volume><issue>5</issue><fpage>1725</fpage><lpage>1738</lpage><history><date date-type="accepted"><day>6</day><month>2</month><year>2007</year></date></history><permissions><copyright-statement>Copyright © American Society for Investigative Pathology</copyright-statement><copyright-year>2007</copyright-year></permissions><self-uri xlink:title="pdf" xlink:href="zjh00507001725.pdf"></self-uri><abstract><p>Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) are both characterized pathologically by the presence of neuronal inclusions termed Lewy bodies (LBs). A common feature found in LBs are aggregates of α-synuclein (α-Syn), and although it is now recognized that α-Syn is the major building block for these toxic filaments, the mechanism of how this occurs remains unknown. In the present study, we demonstrate that proteolytic processing of α-Syn by the protease calpain I leads to the formation of aggregated high-molecular weight species and adoption of a β-sheet structure. To determine whether calpain-cleavage of α-Syn occurs in PD and DLB, we designed site-directed calpain-cleavage antibodies to α-Syn and tested their utility in several animal model systems. Detection of calpain-cleaved α-Syn was evident in mouse models of cerebral ischemia and PD and in a <bold><italic>Drosophila</italic></bold> model of PD. In the human PD and DLB brain, calpain-cleaved α-Syn antibodies immunolabeled LBs and neurites in the substantia nigra. Moreover, calpain-cleaved α-Syn fragments identified within LBs colocalized with activated calpain in neurons of the PD and DLB brains. These findings suggest that calpain I may participate in the disease-linked aggregation of α-Syn in various α-synucleinopathies.</p></abstract></article-meta></front><floats-wrap><fig position="float" id="F1-7229"><label>Figure 1</label><caption><p>Peptide sequences used to generate site-directed calpain-cleavage Abs and locations of the epitopes for the full-length anti-α-Syn Abs used in the present study. Sequences between amino acids 10 and 18 or 117 and 122 were used to synthesize peptides and generate polyclonal Abs as described in Materials and Methods. The N-terminal site was chosen based on sequence analysis after digestion of recombinant α-Syn with calpain I, whereas the C-terminal site was chosen based on a previous report demonstrating the cleavage of α-Syn between amino acids 122 and 123.<xref rid="B19-7229" ref-type="bibr">19</xref> Reported epitopes for the two full-length anti-α-Syn Abs (LB509 and AB5038) used in this study are also shown.</p></caption><graphic xlink:href="zjh0050772290001"></graphic></fig><fig position="float" id="F2-7229"><label>Figure 2</label><caption><p>Calpain cleavage of α-synuclein results in aggregation and adoption of a β-sheet configuration. <bold>A:</bold> After incubation of α-Syn with calpain I for various periods of time in a cell-free assay, samples were subjected to gel electrophoresis, transferred to nitrocellulose filters, and probed with an antibody to FL α-Syn (AB5038). Over time, the appearance of low- and high-molecular weight bands were evident in addition to the disappearance of FL α-Syn (14 kd). <bold>B:</bold> Analytical ultracentrifugation analysis after incubation of α-Syn alone (line on the left) or after digestion with calpain I (line on the right) for 30 minutes indicated the presence of high-molecular weight species. <bold>Insets</bold> represent the 30 raw data scans used for data analysis. <bold>C:</bold> CD spectra after incubation of α-Syn with calpain I indicated a change in structure from random coil to a β-sheet configuration. CD spectra of α-Syn alone (top line) and mixture of α-Syn incubated with calpain I with the calpain spectrum subtracted (bottom line).</p></caption><graphic xlink:href="zjh0050772290002"></graphic></fig><fig position="float" id="F3-7229"><label>Figure 3</label><caption><p>Calpain cleavage of α-Syn results in aggregation and formation of high-molecular weight species. <bold>A:</bold> After incubation of α-Syn with calpain I for various periods of time in a cell-free assay, samples were subjected to gel electrophoresis, transferred to polyvinylidene difluoride filters, and stained with Coomassie blue. Over time, the appearance of low- and high-molecular weight bands were evident in addition to the disappearance of full-length α-Syn (14 kd). <bold>B:</bold> Western blot analysis reveals the presence of high-molecular weight bands after digestion of α-Syn with calpain I in a cell-free system. α-Syn was incubated with calpain I for the indicated times, and samples representing the soluble fractions were immunoblotted using the α-SynCCP Ab. The <bold>arrowhead</bold> indicates the immunoreactivity of the α-SynCCP Ab with a 10-kd fragment of α-Syn. <bold>C:</bold> Western blot analysis using the α-SynCCP Ab demonstrated the presence of calpain-cleaved aggregates of α-Syn after treatment of SY5Y neuroblastoma cells with the calcium ionophore A23187 (<bold>C</bold>, lanes marked 2 and 4 hours), which were prevented after pretreatment of cells with a calpain I inhibitor (<bold>D</bold>, far right lane). <bold>E:</bold> Detection of numerous high-molecular weight calpain-cleavage aggregates of α-Syn in the DLB brain by Western blot analysis. Human brain lysates from age-matched controls or DLB cases were probed with the α-SynCCP antibody. <bold>Arrowheads</bold> above 10 kd indicate the presence of bands between 30 and 80 kd.</p></caption><graphic xlink:href="zjh0050772290003"></graphic></fig><fig position="float" id="F4-7229"><label>Figure 4</label><caption><p>Detection of calpain-cleaved α-Syn in an animal model of cerebral ischemia. Mice were subjected to 1 hour of MCAO, followed by reperfusion for 24 hours. Sections at 50 μm were subjected to either IF or IH analysis using the α-SynCCP Ab (1:100) to detect calpain-cleaved α-Syn. Staining of shrunken, damaged neurons in the cortex of MCAO mice was evident only in ischemic infarct areas (<bold>B</bold>, fluorescence, and <bold>D</bold>, bright-field DAB labeling) and was not evident on the contralateral side of the brain (<bold>A</bold> and <bold>C</bold>). Scale bars = 10 μm.</p></caption><graphic xlink:href="zjh0050772290004"></graphic></fig><fig position="float" id="F5-7229"><label>Figure 5</label><caption><p>Detection of calpain-cleaved α-Syn in a transgenic mouse model of PD. Fixed-tissue brain sections from age-matched non-Tg controls in the hippocamus (<bold>A</bold>) or cortex (<bold>B</bold>) immunolabeled with the α-SynCCP Ab (1:100). <bold>C</bold> and <bold>D:</bold> Cortical staining from 4-month-old asymptomatic PD Tg mice (<bold>C</bold>) or 12-month-old PD Tg mice (<bold>D</bold>) was immunolabeled with the α-SynCCP Ab (1:100). Scale bars = 10 μm.</p></caption><graphic xlink:href="zjh0050772290005"></graphic></fig><fig position="float" id="F6-7229"><label>Figure 6</label><caption><p>Detection of calpain-cleaved species of α-Syn in PD and DLB. <bold>A</bold> and <bold>B:</bold> Representative immunohistochemical single-label staining in the substantia nigra of a DLB (<bold>A</bold>) and PD (<bold>B</bold>) case after application of the α-SynCCP Ab demonstrates the presence of calpain-cleaved α-Syn within Lewy bodies. <bold>C</bold> and <bold>D:</bold> Double-label immunohistochemical analysis with α-SynCCP Ab (black) together with a marker for Lewy bodies (LB509, orange) reveals colocalization in Lewy bodies from a representative DLB (<bold>C</bold>) or PD (<bold>D</bold>) case. Colocalization was also observed in Lewy neurites in DLB (<bold>E</bold>, <bold>arrows</bold>) or PD (<bold>F</bold>) cases. <bold>G</bold> and <bold>H:</bold> Graphical representations depicting the relationship between calpain-cleaved α-Syn and the number of LBs/neurites in five different DLB (<bold>left</bold>) or PD (<bold>right</bold>) cases. Data indicate a high degree of association between the total number of LBs/neurites and the presence of calpain-cleaved α-Syn. <bold>I–N:</bold> Double-label immunofluorescence images for α-SynCCP (green) and an antibody to full-length α-Syn (red) confirmed the colocalization of calpain-cleaved α-Syn within Lewy bodies in a representative DLB (<bold>I–K</bold>) or PD (<bold>L–N</bold>) case. Yellow/orange coloring in <bold>K</bold> and <bold>N</bold> indicate areas where markers are overlapping. <bold>O–T:</bold> Double-label immunofluorescence images for α-SynCCP (green) and calpain I (red) demonstrating the presence of active fragments of the enzyme surrounding Lewy bodies containing calpain-cleaved α-Syn. <bold>O–Q</bold> and <bold>R–T:</bold> Representative staining of a DLB or PD case, respectively. Yellow/orange coloring in <bold>Q</bold> and <bold>T</bold> indicate areas where markers are overlapping. <bold>Asterisks</bold> in <bold>N</bold> denote regions containing neuromelanin. All scale bars = 10 μm.</p></caption><graphic xlink:href="zjh0050772290006"></graphic></fig><fig position="float" id="F7-7229"><label>Figure 7</label><caption><p>Evidence for C-terminal truncation of α-Syn in the DLB brain. <bold>A</bold> and <bold>B:</bold> Characterization of the C-terminal α-SynCCP Ab by Western blot analysis. Recombinant human α-Syn (20 μg) was incubated for various periods of time with calpain I (2 U), separated by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels, transferred to nitrocellulose, and probed with an antibody directed toward a predicted C-terminal calpain-cleavage site (PVDPDN) of α-Syn (<bold>A</bold>). The antibody immunolabeled the predicted 10-kd fragment of α-Syn. <bold>B:</bold> Representative immunohistochemical single-label staining of LBs in the substantia nigra of two DLB cases after application of the C-terminal α-SynCCP Ab. <bold>C:</bold> Identical to <bold>B</bold>, showing labeling with a single Lewy neurite. <bold>D</bold> and <bold>E:</bold> Double-label immunohistochemical analysis in a representative DLB case showing localization of the C-terminal α-SynCCP Ab within LBs and neurites (nickel DAB), which colocalized with a marker for LBs (Nova Red). <bold>F–K:</bold> Immunofluorescence with the C-terminal α-SynCCP Ab shown in green (<bold>G</bold> and <bold>J</bold>), FL α-Syn Ab (LB509) in red (<bold>F</bold> and <bold>I</bold>), and the overlap image (<bold>H</bold> and <bold>K</bold>). All scale bars = 10 μm.</p></caption><graphic xlink:href="zjh0050772290007"></graphic></fig><fig position="float" id="F8-7229"><label>Figure 8</label><caption><p>Comparison of C-terminal calpain-cleaved α-Syn immunoreactivity from several different animal models of PD. <bold>A–F:</bold> Panels are from vibratome sections from the brains of mice immunostained with either the C-terminal α-SynCCP Ab (<bold>A–C</bold>) or a commercially available FL α-Syn Ab from Chemicon AB5038 (<bold>D–F</bold>). After application of the C-terminal α-SynCCP Ab, immunoreactivity within neurons of the neocortex was observed in two different transgenic mice expressing wild-type α-Syn under a PDGF promoter (<bold>B</bold>) or in a line expressing α-Syn under Thy-1 promoter (<bold>C</bold>). <bold>E</bold> and <bold>F:</bold> A similar staining profile was observed using the FL α-Syn Ab. <bold>A</bold> and <bold>D:</bold> No staining with either antibody was observed in non-Tg control mice. <bold>G</bold> and <bold>H:</bold> Detection of the C-terminal calpain-cleavage fragment of α-Syn in a <italic>Drosophila</italic> model of PD. Paraffin-embedded frontal brain sections at the level of the mushroom bodies from 20-day-old flies were immunostained with the C-terminal α-SynCCP Ab (DAB staining) and counterstained with hematoxylin. <bold>G:</bold> The control genotype (<italic>elav-GAL</italic>/+). <bold>H:</bold> α-Syn expressed in a pan-neural pattern under the control of the <italic>elav-GAL</italic> driver. <bold>Arrows</bold>, the peripheral cortex; <bold>asterisks</bold>, the inner neuropil.</p></caption><graphic xlink:href="zjh0050772290008"></graphic></fig><fig position="float" id="F9-7229"><label>Figure 9</label><caption><p>Detection of the C-terminal calpain-cleavage fragment of α-Syn by Western blot analysis. Cytosolic and membrane fractions were prepared from nonTg mice (<bold>lanes 1</bold> and <bold>2</bold>), Tg mice overexpressing wild-type or mutant A53T α-Syn under the PDGF promoter (<bold>lanes 3</bold> and <bold>4</bold>), or from two representative DLB cases (<bold>lanes 5</bold> and <bold>6</bold>). Blots were probed either with the C-terminal α-SynCCP Ab (<bold>A</bold>) or with mAb LB509 (<bold>B</bold>). The C-terminal fragment of α-Syn was detected by α-SynCCP Ab in soluble fractions but not by LB509 (band running just under full-length, 14 kd). <bold>C:</bold> IP experiments with the C-terminal α-SynCCP Ab incubated with control α-Syn (<bold>lane 1</bold>) or digested with calpain I (<bold>lane 2</bold>) under nondenaturing or denaturing conditions. Data suggest the C-terminal α-SynCCP Ab is a conformational-specific Ab whose epitope is lost after denaturation of α-Syn.</p></caption><graphic xlink:href="zjh0050772290009"></graphic></fig><table-wrap position="float" id="T1-7229"><label>Table 1</label><caption><p>Case Demographics for Immunohistochemistry and Western Blot Analyses</p></caption><table frame="hsides" rules="groups"><thead><tr><th colspan="1" rowspan="1" align="center" valign="bottom">Case</th><th colspan="1" rowspan="1" align="center" valign="bottom">Neuropathological diagnosis</th><th colspan="1" rowspan="1" align="center" valign="bottom">Sex</th><th colspan="1" rowspan="1" align="center" valign="bottom">Age (years)</th><th colspan="1" rowspan="1" align="center" valign="bottom">PMI (hours)</th></tr></thead><tbody><tr><td>1 (IH)</td><td>DLB</td><td>M</td><td>88</td><td>2.0</td></tr><tr><td>2 (IH)</td><td>DLB</td><td>M</td><td>75</td><td>6.0</td></tr><tr><td>3 (IH)</td><td>DLB</td><td>M</td><td>67</td><td>2.5</td></tr><tr><td>4 (IH)</td><td>DLB</td><td>M</td><td>72</td><td>5.5</td></tr><tr><td>5 (IH)</td><td>DLB</td><td>F</td><td>77</td><td>2.8</td></tr><tr><td>6 (WB)</td><td>DLB/AD</td><td>F</td><td>77</td><td>5.4</td></tr><tr><td>7 (WB)</td><td>DLB</td><td>M</td><td>85</td><td>4.9</td></tr><tr><td>8 (WB)</td><td>DLB</td><td>M</td><td>71</td><td>2.6</td></tr><tr><td>9 (WB)</td><td>DLB</td><td>M</td><td>70</td><td>3.6</td></tr><tr><td>10 (WB)</td><td>DLB</td><td>M</td><td>83</td><td>9.5</td></tr><tr><td>Mean</td><td></td><td></td><td>76.5 ± 6.93</td><td>4.48 ± 2.28</td></tr><tr><td>1 (IH)</td><td>PD/AD</td><td>F</td><td>88</td><td>3.0</td></tr><tr><td>2 (IH)</td><td>PD</td><td>F</td><td>86</td><td>13.5</td></tr><tr><td>3 (IH)</td><td>PD/AD</td><td>M</td><td>64</td><td>2.3</td></tr><tr><td>4 (IH)</td><td>PD</td><td>M</td><td>69</td><td>4.5</td></tr><tr><td>5 (IH)</td><td>PD</td><td>M</td><td>71</td><td>3.5</td></tr><tr><td>Mean</td><td></td><td></td><td>75.6 ± 10.7</td><td>5.36 ± 4.62</td></tr><tr><td>1 (WB)</td><td>CTL</td><td>F</td><td>73</td><td>4.0</td></tr><tr><td>2 (WB)</td><td>CTL</td><td>M</td><td>64</td><td>7.0</td></tr><tr><td>3 (WB)</td><td>CTL</td><td>M</td><td>77</td><td>6.5</td></tr><tr><td>4 (WB)</td><td>CTL</td><td>F</td><td>74</td><td>2.8</td></tr><tr><td>Mean</td><td></td><td></td><td>72 ± 5.59</td><td>5.08 ± 2.00</td></tr></tbody></table><table-wrap-foot><fn><p>DLB cases 1 to 5 were used for IH analysis, whereas cases 6 to 10 and CTL cases were used for Western blot analysis (WB). PMI, postmortem interval; M, male; F, female; AD, Alzheimer’s disease; CTL, age-matched control cases. </p></fn></table-wrap-foot></table-wrap></floats-wrap></pmc><affiliations><list></list><tree><noCountry><name sortKey="Dufty, Brian M" sort="Dufty, Brian M" uniqKey="Dufty B" first="Brian M." last="Dufty">Brian M. Dufty</name><name sortKey="Feany, Mel B" sort="Feany, Mel B" uniqKey="Feany M" first="Mel B." last="Feany">Mel B. Feany</name><name sortKey="Gomez Isla, Teresa" sort="Gomez Isla, Teresa" uniqKey="Gomez Isla T" first="Teresa" last="Gomez-Isla">Teresa Gomez-Isla</name><name sortKey="Hou, Sheng T" sort="Hou, Sheng T" uniqKey="Hou S" first="Sheng T." last="Hou">Sheng T. Hou</name><name sortKey="Jiang, Susan X" sort="Jiang, Susan X" uniqKey="Jiang S" first="Susan X." last="Jiang">Susan X. Jiang</name><name sortKey="Leenhouts, Kristen M" sort="Leenhouts, Kristen M" uniqKey="Leenhouts K" first="Kristen M." last="Leenhouts">Kristen M. Leenhouts</name><name sortKey="Masliah, Eliezer" sort="Masliah, Eliezer" uniqKey="Masliah E" first="Eliezer" last="Masliah">Eliezer Masliah</name><name sortKey="Oxford, Julia T" sort="Oxford, Julia T" uniqKey="Oxford J" first="Julia T." last="Oxford">Julia T. Oxford</name><name sortKey="Rohn, Troy T" sort="Rohn, Troy T" uniqKey="Rohn T" first="Troy T." last="Rohn">Troy T. Rohn</name><name sortKey="Warner, Lisa R" sort="Warner, Lisa R" uniqKey="Warner L" first="Lisa R." last="Warner">Lisa R. Warner</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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