Mural granulosa cell gene expression associated with oocyte developmental competence
Identifieur interne : 000A29 ( Pmc/Checkpoint ); précédent : 000A28; suivant : 000A30Mural granulosa cell gene expression associated with oocyte developmental competence
Auteurs : Jin-Yi Jiang [Canada] ; Huiling Xiong [Canada] ; Mingju Cao [Canada] ; Xuhua Xia [Canada] ; Marc-Andre Sirard [Canada] ; Benjamin K. Tsang [Canada, Corée du Sud]Source :
- Journal of Ovarian Research [ 1757-2215 ] ; 2010.
Abstract
Ovarian follicle development is a complex process. Paracrine interactions between somatic and germ cells are critical for normal follicular development and oocyte maturation. Studies have suggested that the health and function of the granulosa and cumulus cells may be reflective of the health status of the enclosed oocyte. The objective of the present study is to assess, using an
Immature rats were injected with eCG and 24 h thereafter with anti-eCG antibody to induce follicular atresia or with pre-immune serum to stimulate follicle development. A high percentage (30-50%, normal developmental competence, NDC) of oocytes from eCG/pre-immune serum group developed to term after embryo transfer compared to those from eCG/anti-eCG (0%, poor developmental competence, PDC). Gene expression profiles of mural granulosa cells from the above oocyte-collected follicles were assessed by Affymetrix rat whole genome array.
The result showed that twelve genes were up-regulated, while one gene was down-regulated more than 1.5 folds in the NDC group compared with those in the PDC group. Gene ontology classification showed that the up-regulated genes included lysyl oxidase (
The data in the present study demonstrate a close association between specific gene expression in mural granulosa cells and the developmental competence of oocytes. This finding suggests that the most differentially expressed gene, lysyl oxidase, may be a candidate biomarker of oocyte health and useful for the selection of good quality oocytes for assisted reproduction.
Url:
DOI: 10.1186/1757-2215-3-6
PubMed: 20205929
PubMed Central: 2845131
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:2845131Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mural granulosa cell gene expression associated with oocyte developmental competence</title><author><name sortKey="Jiang, Jin Yi" sort="Jiang, Jin Yi" uniqKey="Jiang J" first="Jin-Yi" last="Jiang">Jin-Yi Jiang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9</wicri:regionArea><wicri:noRegion>ON K1Y 4E9</wicri:noRegion></affiliation></author><author><name sortKey="Xiong, Huiling" sort="Xiong, Huiling" uniqKey="Xiong H" first="Huiling" last="Xiong">Huiling Xiong</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5</wicri:regionArea><wicri:noRegion>ON K1N 6N5</wicri:noRegion></affiliation></author><author><name sortKey="Cao, Mingju" sort="Cao, Mingju" uniqKey="Cao M" first="Mingju" last="Cao">Mingju Cao</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9</wicri:regionArea><wicri:noRegion>ON K1Y 4E9</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Xuhua" sort="Xia, Xuhua" uniqKey="Xia X" first="Xuhua" last="Xia">Xuhua Xia</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5</wicri:regionArea><wicri:noRegion>ON K1N 6N5</wicri:noRegion></affiliation></author><author><name sortKey="Sirard, Marc Andre" sort="Sirard, Marc Andre" uniqKey="Sirard M" first="Marc-Andre" last="Sirard">Marc-Andre Sirard</name><affiliation wicri:level="1"><nlm:aff id="I3">Centre de Recherche en Biologie de la Reproduction, Département de Sciences Animales, Université Laval, Ste-Foy, QuébecG1K 7P4, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de Recherche en Biologie de la Reproduction, Département de Sciences Animales, Université Laval, Ste-Foy, QuébecG1K 7P4</wicri:regionArea><wicri:noRegion>QuébecG1K 7P4</wicri:noRegion></affiliation></author><author><name sortKey="Tsang, Benjamin K" sort="Tsang, Benjamin K" uniqKey="Tsang B" first="Benjamin K" last="Tsang">Benjamin K. Tsang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9</wicri:regionArea><wicri:noRegion>ON K1Y 4E9</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="I4">WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><settlement type="city">Séoul</settlement></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20205929</idno><idno type="pmc">2845131</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845131</idno><idno type="RBID">PMC:2845131</idno><idno type="doi">10.1186/1757-2215-3-6</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000A25</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A25</idno><idno type="wicri:Area/Pmc/Curation">000A25</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A25</idno><idno type="wicri:Area/Pmc/Checkpoint">000A29</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A29</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Mural granulosa cell gene expression associated with oocyte developmental competence</title><author><name sortKey="Jiang, Jin Yi" sort="Jiang, Jin Yi" uniqKey="Jiang J" first="Jin-Yi" last="Jiang">Jin-Yi Jiang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9</wicri:regionArea><wicri:noRegion>ON K1Y 4E9</wicri:noRegion></affiliation></author><author><name sortKey="Xiong, Huiling" sort="Xiong, Huiling" uniqKey="Xiong H" first="Huiling" last="Xiong">Huiling Xiong</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5</wicri:regionArea><wicri:noRegion>ON K1N 6N5</wicri:noRegion></affiliation></author><author><name sortKey="Cao, Mingju" sort="Cao, Mingju" uniqKey="Cao M" first="Mingju" last="Cao">Mingju Cao</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9</wicri:regionArea><wicri:noRegion>ON K1Y 4E9</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Xuhua" sort="Xia, Xuhua" uniqKey="Xia X" first="Xuhua" last="Xia">Xuhua Xia</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5</wicri:regionArea><wicri:noRegion>ON K1N 6N5</wicri:noRegion></affiliation></author><author><name sortKey="Sirard, Marc Andre" sort="Sirard, Marc Andre" uniqKey="Sirard M" first="Marc-Andre" last="Sirard">Marc-Andre Sirard</name><affiliation wicri:level="1"><nlm:aff id="I3">Centre de Recherche en Biologie de la Reproduction, Département de Sciences Animales, Université Laval, Ste-Foy, QuébecG1K 7P4, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de Recherche en Biologie de la Reproduction, Département de Sciences Animales, Université Laval, Ste-Foy, QuébecG1K 7P4</wicri:regionArea><wicri:noRegion>QuébecG1K 7P4</wicri:noRegion></affiliation></author><author><name sortKey="Tsang, Benjamin K" sort="Tsang, Benjamin K" uniqKey="Tsang B" first="Benjamin K" last="Tsang">Benjamin K. Tsang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9</wicri:regionArea><wicri:noRegion>ON K1Y 4E9</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="I4">WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><settlement type="city">Séoul</settlement></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author></analytic><series><title level="j">Journal of Ovarian Research</title><idno type="eISSN">1757-2215</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Ovarian follicle development is a complex process. Paracrine interactions between somatic and germ cells are critical for normal follicular development and oocyte maturation. Studies have suggested that the health and function of the granulosa and cumulus cells may be reflective of the health status of the enclosed oocyte. The objective of the present study is to assess, using an <italic>in vivo </italic>immature rat model, gene expression profile in granulosa cells, which may be linked to the developmental competence of the oocyte. We hypothesized that expression of specific genes in granulosa cells may be correlated with the developmental competence of the oocyte.</p></sec><sec><title>Methods</title><p>Immature rats were injected with eCG and 24 h thereafter with anti-eCG antibody to induce follicular atresia or with pre-immune serum to stimulate follicle development. A high percentage (30-50%, normal developmental competence, NDC) of oocytes from eCG/pre-immune serum group developed to term after embryo transfer compared to those from eCG/anti-eCG (0%, poor developmental competence, PDC). Gene expression profiles of mural granulosa cells from the above oocyte-collected follicles were assessed by Affymetrix rat whole genome array.</p></sec><sec><title>Results</title><p>The result showed that twelve genes were up-regulated, while one gene was down-regulated more than 1.5 folds in the NDC group compared with those in the PDC group. Gene ontology classification showed that the up-regulated genes included lysyl oxidase (<italic>Lox</italic>) and nerve growth factor receptor associated protein 1 (<italic>Ngfrap1</italic>), which are important in the regulation of protein-lysine 6-oxidase activity, and in apoptosis induction, respectively. The down-regulated genes included glycoprotein-4-beta galactosyltransferase 2 (<italic>Ggbt2</italic>), which is involved in the regulation of extracellular matrix organization and biogenesis.</p></sec><sec><title>Conclusions</title><p>The data in the present study demonstrate a close association between specific gene expression in mural granulosa cells and the developmental competence of oocytes. This finding suggests that the most differentially expressed gene, lysyl oxidase, may be a candidate biomarker of oocyte health and useful for the selection of good quality oocytes for assisted reproduction.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carabatsos, Mj" uniqKey="Carabatsos M">MJ Carabatsos</name></author><author><name sortKey="Sellitto, C" uniqKey="Sellitto C">C Sellitto</name></author><author><name sortKey="Goodenough, Da" uniqKey="Goodenough D">DA Goodenough</name></author><author><name sortKey="Albertini, Df" uniqKey="Albertini D">DF Albertini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brower, Pt" uniqKey="Brower P">PT Brower</name></author><author><name sortKey="Schultz, Rm" uniqKey="Schultz R">RM Schultz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomas, Fh" uniqKey="Thomas F">FH Thomas</name></author><author><name sortKey="Vanderhyden, Bc" uniqKey="Vanderhyden B">BC Vanderhyden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chesnel, F" uniqKey="Chesnel F">F Chesnel</name></author><author><name sortKey="Wigglesworth, K" uniqKey="Wigglesworth K">K Wigglesworth</name></author><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De La Fuente, R" uniqKey="De La Fuente R">R de la Fuente</name></author><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matzuk, Mm" uniqKey="Matzuk M">MM Matzuk</name></author><author><name sortKey="Burns, Kh" uniqKey="Burns K">KH Burns</name></author><author><name sortKey="Viveiros, Mm" uniqKey="Viveiros M">MM Viveiros</name></author><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vanderhyden, Bc" uniqKey="Vanderhyden B">BC Vanderhyden</name></author><author><name sortKey="Telfer, Ee" uniqKey="Telfer E">EE Telfer</name></author><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vanderhyden, Bc" uniqKey="Vanderhyden B">BC Vanderhyden</name></author><author><name sortKey="Caron, Pj" uniqKey="Caron P">PJ Caron</name></author><author><name sortKey="Buccione, R" uniqKey="Buccione R">R Buccione</name></author><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buccione, R" uniqKey="Buccione R">R Buccione</name></author><author><name sortKey="Vanderhyden, Bc" uniqKey="Vanderhyden B">BC Vanderhyden</name></author><author><name sortKey="Caron, Pj" uniqKey="Caron P">PJ Caron</name></author><author><name sortKey="Eppig, Jj" uniqKey="Eppig J">JJ Eppig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otsuka, F" uniqKey="Otsuka F">F Otsuka</name></author><author><name sortKey="Yamamoto, S" uniqKey="Yamamoto S">S Yamamoto</name></author><author><name sortKey="Erickson, Gf" uniqKey="Erickson G">GF Erickson</name></author><author><name sortKey="Shimasaki, S" uniqKey="Shimasaki S">S Shimasaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Orisaka, M" uniqKey="Orisaka M">M Orisaka</name></author><author><name sortKey="Orisaka, S" uniqKey="Orisaka S">S Orisaka</name></author><author><name sortKey="Jiang, Jy" uniqKey="Jiang J">JY Jiang</name></author><author><name sortKey="Craig, J" uniqKey="Craig J">J Craig</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Kotsuji, F" uniqKey="Kotsuji F">F Kotsuji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hunter, Mg" uniqKey="Hunter M">MG Hunter</name></author><author><name sortKey="Brankin, V" uniqKey="Brankin V">V Brankin</name></author><author><name sortKey="Quinn, Rl" uniqKey="Quinn R">RL Quinn</name></author><author><name sortKey="Ferguson, Em" uniqKey="Ferguson E">EM Ferguson</name></author><author><name sortKey="Edwards, Sa" uniqKey="Edwards S">SA Edwards</name></author><author><name sortKey="Ashworth, Cj" uniqKey="Ashworth C">CJ Ashworth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mckenzie, Lj" uniqKey="Mckenzie L">LJ McKenzie</name></author><author><name sortKey="Pangas, Sa" uniqKey="Pangas S">SA Pangas</name></author><author><name sortKey="Carson, Sa" uniqKey="Carson S">SA Carson</name></author><author><name sortKey="Kovanci, E" uniqKey="Kovanci E">E Kovanci</name></author><author><name sortKey="Cisneros, P" uniqKey="Cisneros P">P Cisneros</name></author><author><name sortKey="Buster, Je" uniqKey="Buster J">JE Buster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rhodes, Tl" uniqKey="Rhodes T">TL Rhodes</name></author><author><name sortKey="Mccoy, Tp" uniqKey="Mccoy T">TP McCoy</name></author><author><name sortKey="Higdon, Hl" uniqKey="Higdon H">HL Higdon</name></author><author><name sortKey="Boone, Wr" uniqKey="Boone W">WR Boone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Racowsky, C" uniqKey="Racowsky C">C Racowsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, Jy" uniqKey="Jiang J">JY Jiang</name></author><author><name sortKey="Umezu, M" uniqKey="Umezu M">M Umezu</name></author><author><name sortKey="Sato, E" uniqKey="Sato E">E Sato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, Jy" uniqKey="Jiang J">JY Jiang</name></author><author><name sortKey="Miyoshi, K" uniqKey="Miyoshi K">K Miyoshi</name></author><author><name sortKey="Umezu, M" uniqKey="Umezu M">M Umezu</name></author><author><name sortKey="Sato, E" uniqKey="Sato E">E Sato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mihm, M" uniqKey="Mihm M">M Mihm</name></author><author><name sortKey="Baker, Pj" uniqKey="Baker P">PJ Baker</name></author><author><name sortKey="Ireland, Jl" uniqKey="Ireland J">JL Ireland</name></author><author><name sortKey="Smith, Gw" uniqKey="Smith G">GW Smith</name></author><author><name sortKey="Coussens, Pm" uniqKey="Coussens P">PM Coussens</name></author><author><name sortKey="Evans, Ac" uniqKey="Evans A">AC Evans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boone, Dl" uniqKey="Boone D">DL Boone</name></author><author><name sortKey="Carnegie, Ja" uniqKey="Carnegie J">JA Carnegie</name></author><author><name sortKey="Rippstein, Pu" uniqKey="Rippstein P">PU Rippstein</name></author><author><name sortKey="Tsang, Bk" uniqKey="Tsang B">BK Tsang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Jm" uniqKey="Kim J">JM Kim</name></author><author><name sortKey="Boone, Dl" uniqKey="Boone D">DL Boone</name></author><author><name sortKey="Auyeung, A" uniqKey="Auyeung A">A Auyeung</name></author><author><name sortKey="Tsang, Bk" uniqKey="Tsang B">BK Tsang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Jiang, Jy" uniqKey="Jiang J">JY Jiang</name></author><author><name sortKey="Zhu, C" uniqKey="Zhu C">C Zhu</name></author><author><name sortKey="Peng, C" uniqKey="Peng C">C Peng</name></author><author><name sortKey="Tsang, Bk" uniqKey="Tsang B">BK Tsang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, Jy" uniqKey="Jiang J">JY Jiang</name></author><author><name sortKey="Tsang, Bk" uniqKey="Tsang B">BK Tsang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bolstad, Bm" uniqKey="Bolstad B">BM Bolstad</name></author><author><name sortKey="Irizarry, Ra" uniqKey="Irizarry R">RA Irizarry</name></author><author><name sortKey="Astrand, M" uniqKey="Astrand M">M Astrand</name></author><author><name sortKey="Speed, Tp" uniqKey="Speed T">TP Speed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Irizarry, Ra" uniqKey="Irizarry R">RA Irizarry</name></author><author><name sortKey="Bolstad, Bm" uniqKey="Bolstad B">BM Bolstad</name></author><author><name sortKey="Collin, F" uniqKey="Collin F">F Collin</name></author><author><name sortKey="Cope, Lm" uniqKey="Cope L">LM Cope</name></author><author><name sortKey="Hobbs, B" uniqKey="Hobbs B">B Hobbs</name></author><author><name sortKey="Speed, Tp" uniqKey="Speed T">TP Speed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pelizzola, M" uniqKey="Pelizzola M">M Pelizzola</name></author><author><name sortKey="Pavelka, N" uniqKey="Pavelka N">N Pavelka</name></author><author><name sortKey="Foti, M" uniqKey="Foti M">M Foti</name></author><author><name sortKey="Ricciardi Castagnoli, P" uniqKey="Ricciardi Castagnoli P">P Ricciardi-Castagnoli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saeed, Ai" uniqKey="Saeed A">AI Saeed</name></author><author><name sortKey="Sharov, V" uniqKey="Sharov V">V Sharov</name></author><author><name sortKey="White, J" uniqKey="White J">J White</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Liang, W" uniqKey="Liang W">W Liang</name></author><author><name sortKey="Bhagabati, N" uniqKey="Bhagabati N">N Bhagabati</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dennis, G" uniqKey="Dennis G">G Dennis</name></author><author><name sortKey="Sherman, Bt" uniqKey="Sherman B">BT Sherman</name></author><author><name sortKey="Hosack, Da" uniqKey="Hosack D">DA Hosack</name></author><author><name sortKey="Yang, J" uniqKey="Yang J">J Yang</name></author><author><name sortKey="Gao, W" uniqKey="Gao W">W Gao</name></author><author><name sortKey="Lane, Hc" uniqKey="Lane H">HC Lane</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Livak, Kj" uniqKey="Livak K">KJ Livak</name></author><author><name sortKey="Schmittgen, Td" uniqKey="Schmittgen T">TD Schmittgen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kendall, Nr" uniqKey="Kendall N">NR Kendall</name></author><author><name sortKey="Marsters, P" uniqKey="Marsters P">P Marsters</name></author><author><name sortKey="Scaramuzzi, Rj" uniqKey="Scaramuzzi R">RJ Scaramuzzi</name></author><author><name sortKey="Campbell, Bk" uniqKey="Campbell B">BK Campbell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nocentini, G" uniqKey="Nocentini G">G Nocentini</name></author><author><name sortKey="Giunchi, L" uniqKey="Giunchi L">L Giunchi</name></author><author><name sortKey="Ronchetti, S" uniqKey="Ronchetti S">S Ronchetti</name></author><author><name sortKey="Krausz, Lt" uniqKey="Krausz L">LT Krausz</name></author><author><name sortKey="Bartoli, A" uniqKey="Bartoli A">A Bartoli</name></author><author><name sortKey="Moraca, R" uniqKey="Moraca R">R Moraca</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asano, M" uniqKey="Asano M">M Asano</name></author><author><name sortKey="Furukawa, K" uniqKey="Furukawa K">K Furukawa</name></author><author><name sortKey="Kido, M" uniqKey="Kido M">M Kido</name></author><author><name sortKey="Matsumoto, S" uniqKey="Matsumoto S">S Matsumoto</name></author><author><name sortKey="Umesaki, Y" uniqKey="Umesaki Y">Y Umesaki</name></author><author><name sortKey="Kochibe, N" uniqKey="Kochibe N">N Kochibe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harlow, Cr" uniqKey="Harlow C">CR Harlow</name></author><author><name sortKey="Rae, M" uniqKey="Rae M">M Rae</name></author><author><name sortKey="Davidson, L" uniqKey="Davidson L">L Davidson</name></author><author><name sortKey="Trackman, Pc" uniqKey="Trackman P">PC Trackman</name></author><author><name sortKey="Hillier, Sg" uniqKey="Hillier S">SG Hillier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Nellaiappan, K" uniqKey="Nellaiappan K">K Nellaiappan</name></author><author><name sortKey="Strassmaier, T" uniqKey="Strassmaier T">T Strassmaier</name></author><author><name sortKey="Graham, L" uniqKey="Graham L">L Graham</name></author><author><name sortKey="Thomas, Km" uniqKey="Thomas K">KM Thomas</name></author><author><name sortKey="Kagan, Hm" uniqKey="Kagan H">HM Kagan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kagan, Hm" uniqKey="Kagan H">HM Kagan</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mello, Ml" uniqKey="Mello M">ML Mello</name></author><author><name sortKey="Contente, S" uniqKey="Contente S">S Contente</name></author><author><name sortKey="Vidal, Bc" uniqKey="Vidal B">BC Vidal</name></author><author><name sortKey="Planding, W" uniqKey="Planding W">W Planding</name></author><author><name sortKey="Schenck, U" uniqKey="Schenck U">U Schenck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grattarola, M" uniqKey="Grattarola M">M Grattarola</name></author><author><name sortKey="Borghi, C" uniqKey="Borghi C">C Borghi</name></author><author><name sortKey="Emionite, L" uniqKey="Emionite L">L Emionite</name></author><author><name sortKey="Lulli, P" uniqKey="Lulli P">P Lulli</name></author><author><name sortKey="Chessa, L" uniqKey="Chessa L">L Chessa</name></author><author><name sortKey="Vergani, L" uniqKey="Vergani L">L Vergani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slee, Rb" uniqKey="Slee R">RB Slee</name></author><author><name sortKey="Hillier, Sg" uniqKey="Hillier S">SG Hillier</name></author><author><name sortKey="Largue, P" uniqKey="Largue P">P Largue</name></author><author><name sortKey="Harlow, Cr" uniqKey="Harlow C">CR Harlow</name></author><author><name sortKey="Miele, G" uniqKey="Miele G">G Miele</name></author><author><name sortKey="Clinton, M" uniqKey="Clinton M">M Clinton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Langenau, Dm" uniqKey="Langenau D">DM Langenau</name></author><author><name sortKey="Goetz, Fw" uniqKey="Goetz F">FW Goetz</name></author><author><name sortKey="Roberts, Sb" uniqKey="Roberts S">SB Roberts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roy, R" uniqKey="Roy R">R Roy</name></author><author><name sortKey="Polgar, P" uniqKey="Polgar P">P Polgar</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Goldstein, Rh" uniqKey="Goldstein R">RH Goldstein</name></author><author><name sortKey="Taylor, L" uniqKey="Taylor L">L Taylor</name></author><author><name sortKey="Kagan, Hm" uniqKey="Kagan H">HM Kagan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turano, C" uniqKey="Turano C">C Turano</name></author><author><name sortKey="Coppari, S" uniqKey="Coppari S">S Coppari</name></author><author><name sortKey="Altieri, F" uniqKey="Altieri F">F Altieri</name></author><author><name sortKey="Ferraro, A" uniqKey="Ferraro A">A Ferraro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knizetova, P" uniqKey="Knizetova P">P Knizetova</name></author><author><name sortKey="Vancova, I" uniqKey="Vancova I">I Vancova</name></author><author><name sortKey="Kocakova, P" uniqKey="Kocakova P">P Kocakova</name></author><author><name sortKey="Slovak, M" uniqKey="Slovak M">M Slovak</name></author><author><name sortKey="Proost, P" uniqKey="Proost P">P Proost</name></author><author><name sortKey="Kopacek, J" uniqKey="Kopacek J">J Kopacek</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Ovarian Res</journal-id><journal-title-group><journal-title>Journal of Ovarian Research</journal-title></journal-title-group><issn pub-type="epub">1757-2215</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20205929</article-id><article-id pub-id-type="pmc">2845131</article-id><article-id pub-id-type="publisher-id">1757-2215-3-6</article-id><article-id pub-id-type="doi">10.1186/1757-2215-3-6</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Mural granulosa cell gene expression associated with oocyte developmental competence</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Jiang</surname><given-names>Jin-Yi</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>jjiang@ohri.ca</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Xiong</surname><given-names>Huiling</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>hlxiong@gmail.com</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Cao</surname><given-names>Mingju</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>mcao@ohri.ca</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Xia</surname><given-names>Xuhua</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>Xuhua.Xia@uottawa.ca</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Sirard</surname><given-names>Marc-Andre</given-names></name><xref ref-type="aff" rid="I3">3</xref><email>Marc-Andre.Sirard@crbr.ulaval.ca</email></contrib><contrib contrib-type="author" corresp="yes" id="A6"><name><surname>Tsang</surname><given-names>Benjamin K</given-names></name><xref ref-type="aff" rid="I1">1</xref><xref ref-type="aff" rid="I4">4</xref><email>btsang@ohri.ca</email></contrib></contrib-group><aff id="I1"><label>1</label>Department of Obstetrics & Gynecology and Cellular & Molecular Medicine, University of Ottawa, Ottawa Hospital Research Institute, Ottawa, ON K1Y 4E9, Canada</aff><aff id="I2"><label>2</label>Department of Biology and Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON K1N 6N5, Canada</aff><aff id="I3"><label>3</label>Centre de Recherche en Biologie de la Reproduction, Département de Sciences Animales, Université Laval, Ste-Foy, QuébecG1K 7P4, Canada</aff><aff id="I4"><label>4</label>WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea</aff><pub-date pub-type="collection"><year>2010</year></pub-date><pub-date pub-type="epub"><day>6</day><month>3</month><year>2010</year></pub-date><volume>3</volume><fpage>6</fpage><lpage>6</lpage><history><date date-type="received"><day>3</day><month>1</month><year>2010</year></date><date date-type="accepted"><day>6</day><month>3</month><year>2010</year></date></history><permissions><copyright-statement>Copyright ©2010 Jiang et al; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2010</copyright-year><copyright-holder>Jiang et al; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.ovarianresearch.com/content/3/1/6"></self-uri><abstract><sec><title>Background</title><p>Ovarian follicle development is a complex process. Paracrine interactions between somatic and germ cells are critical for normal follicular development and oocyte maturation. Studies have suggested that the health and function of the granulosa and cumulus cells may be reflective of the health status of the enclosed oocyte. The objective of the present study is to assess, using an <italic>in vivo </italic>immature rat model, gene expression profile in granulosa cells, which may be linked to the developmental competence of the oocyte. We hypothesized that expression of specific genes in granulosa cells may be correlated with the developmental competence of the oocyte.</p></sec><sec><title>Methods</title><p>Immature rats were injected with eCG and 24 h thereafter with anti-eCG antibody to induce follicular atresia or with pre-immune serum to stimulate follicle development. A high percentage (30-50%, normal developmental competence, NDC) of oocytes from eCG/pre-immune serum group developed to term after embryo transfer compared to those from eCG/anti-eCG (0%, poor developmental competence, PDC). Gene expression profiles of mural granulosa cells from the above oocyte-collected follicles were assessed by Affymetrix rat whole genome array.</p></sec><sec><title>Results</title><p>The result showed that twelve genes were up-regulated, while one gene was down-regulated more than 1.5 folds in the NDC group compared with those in the PDC group. Gene ontology classification showed that the up-regulated genes included lysyl oxidase (<italic>Lox</italic>) and nerve growth factor receptor associated protein 1 (<italic>Ngfrap1</italic>), which are important in the regulation of protein-lysine 6-oxidase activity, and in apoptosis induction, respectively. The down-regulated genes included glycoprotein-4-beta galactosyltransferase 2 (<italic>Ggbt2</italic>), which is involved in the regulation of extracellular matrix organization and biogenesis.</p></sec><sec><title>Conclusions</title><p>The data in the present study demonstrate a close association between specific gene expression in mural granulosa cells and the developmental competence of oocytes. This finding suggests that the most differentially expressed gene, lysyl oxidase, may be a candidate biomarker of oocyte health and useful for the selection of good quality oocytes for assisted reproduction.</p></sec></abstract></article-meta></front></pmc><affiliations><list><country><li>Canada</li><li>Corée du Sud</li></country><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Canada"><noRegion><name sortKey="Jiang, Jin Yi" sort="Jiang, Jin Yi" uniqKey="Jiang J" first="Jin-Yi" last="Jiang">Jin-Yi Jiang</name></noRegion><name sortKey="Cao, Mingju" sort="Cao, Mingju" uniqKey="Cao M" first="Mingju" last="Cao">Mingju Cao</name><name sortKey="Sirard, Marc Andre" sort="Sirard, Marc Andre" uniqKey="Sirard M" first="Marc-Andre" last="Sirard">Marc-Andre Sirard</name><name sortKey="Tsang, Benjamin K" sort="Tsang, Benjamin K" uniqKey="Tsang B" first="Benjamin K" last="Tsang">Benjamin K. Tsang</name><name sortKey="Xia, Xuhua" sort="Xia, Xuhua" uniqKey="Xia X" first="Xuhua" last="Xia">Xuhua Xia</name><name sortKey="Xiong, Huiling" sort="Xiong, Huiling" uniqKey="Xiong H" first="Huiling" last="Xiong">Huiling Xiong</name></country><country name="Corée du Sud"><noRegion><name sortKey="Tsang, Benjamin K" sort="Tsang, Benjamin K" uniqKey="Tsang B" first="Benjamin K" last="Tsang">Benjamin K. Tsang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A29 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000A29 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:2845131
|texte= Mural granulosa cell gene expression associated with oocyte developmental competence
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:20205929" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a ParkinsonCanadaV1
| This area was generated with Dilib version V0.6.29. |  |